Search Results (4,015)

To evaluate the impact of different roughages on the intestinal microbiota of yak calves, we fed them oat hay in substitution of alfalfa hay, in addition to milk replacer and starter powder. Twenty-one 45-day-old male yak calves were selected and randomly assigned to three groups: the milk replacer + starter + alfalfa hay group (AH), the milk replacer + starter + oat hay group (OH), and the milk replacer + starter + mixed hay group (AO), in which the alfalfa hay and oat hay were administered in a 1:1 ratio. All calves in the three groups were fed the same milk replacer and an equivalent amount of dry matter. The formal experiment commenced after a 21-day pre-test period and lasted for 120 days. Following the experiment, the contents of the jejunum and colon were collected to investigate the intestinal microbiota and metabolites using 16S rRNA sequencing and LC-MS metabolomics. The result showed that the AO group had greater final body weights overall than the AH group and OH group (p < 0.05). The AH group and OH group had considerably greater feed-to-gain ratios than the AO group (p < 0.05). At the phylum level, the OH group exhibited an increased relative abundance of Bacteroidota and Spirochaetota in the jejunum (p < 0.05). The relative abundance of Actinobacteriota in the colon was increased in the AO group (p < 0.05). At the genus level, the AO group exhibited a decreased abundance of Clostridium sensu_stricto_1 (p < 0.05), and the OH group showed an increased abundance UCG-005 and Alistipes in the jejunum. There were many differential metabolites in the OH group and AO group compared to the AH group, and the different metabolites of the OH group were associated with the metabolic pathways of the nervous system, sensory system, amino acid metabolism, and lipid metabolism in the jejunum and with lipid metabolism, amino acid metabolism, and the nervous system in the colon. In the AO group, these metabolites were associated with the digestive system and the translation and metabolism of cofactors in the jejunum and with the metabolism of cofactors and vitamins in the colon. In summary, it is feasible to replace alfalfa hay with oat hay based on milk replacer and starter. The combination of the two forages enhanced nutrient absorption, improved immune function, maintained the internal homeostasis of yak calves, and was more beneficial to their growth and development. Full article

The spatial distribution and seasonal variation in heavy metal pollution in road dust in Liuzhou, China, were investigated. Road dust samples were collected during both drought and wet periods, and the concentrations of nine heavy metals—Cr, Ni, Cu, Pb, Zn, Cd, As, Sb, and Hg—were analyzed. The analysis showed that all nine heavy metals were higher than the background values of Chinese urban soils in both the drought and wet periods, and the mean heavy metal contents of road dust in the dry period were higher than those in the wet period, except for Cd. In the assessment of the pollution of heavy metals in road dust, the results of Igeo showed that Cd and Hg were significant; the results of NI showed that Cr and Ni were more significant. The spatial analysis shows that the industrial concentration areas in the north and west of Liuzhou City are concentration areas of heavy metal pollution. A Pearson correlation analysis showed high correlation between Cd in road dust heavy metals and Cd in the surrounding soil. Source identification via PCA revealed four main contributors: metallurgical and coal-fired industries, mechanical manufacturing, green belt maintenance, and waste treatment facilities. A quantitative analysis via APCS-MLR modeling confirmed that metallurgical and coal-fired industrial sources are the most widespread and important pollution sources in Liuzhou. There is a significant increase in the contribution of waste treatment sources to Hg contamination during wet periods. Full article

This study investigates the phenotypic diversity of spring-flowering Crocus species native to Serbia by analyzing their morphological traits and flower color variations. Detailed phenotypic characterization was performed on seven species: C. alexandri, C. chrysanthus, C. heuffelianus, C. randjeloviciorum, C. rujanensis, C. variegatus, and C. weldenii. The study examines how morphological parameters of tested species diverged from average values during a hot and dry winter, resulting in smaller plants, earlier and shorter flowering periods, and reduced flower size, regardless of species. Hierarchical clustering based on morphological traits grouped the species into three clusters: the first cluster included C. alexandri, C. chrysanthus, C. rujanensis, and C. weldenii, characterized by the highest number of leaves and the lowest number of flowers; the second consisted of C. randjeloviciorum, distinguished by shorter plant height and the greatest number of flowers; and the third cluster comprised C. heuffelianus and C. variegatus, notable for the tallest plants, widest corms and leaves, and longest perigon segments. Grouping based on petal color components (L*, a*, b*, chroma, and hue) formed five distinct clusters, demonstrating differences in flower color. These findings are valuable for taxonomic classification, conservation measures, and breeding programs, which ultimately support the conservation of biodiversity and the promotion of ornamental plant cultivation. Flower color in particular has proven to be a reliable species indicator, as it remains constant despite the year and/or habitat. The use of a colorimeter can speed up the identification of species in the field and provides researchers and conservationists with a practical tool. Full article

Drought has become an important factor affecting the environment and socio-economic sustainable development in northern China due to climate change. This study utilized the Standardized Precipitation Index (SPI) as a drought metric to investigate the correlation between drought characteristics and different grades of precipitation and rain days. The analysis was based on a long-term time series of precipitation data obtained from 116 meteorological stations located in Inner Mongolia, spanning 1960 to 2019. To achieve the objectives of the current research, the daily precipitation was categorized into four grades based on the “24-h Precipitation Classification Standard”, and the frequency of rain days for each grade was determined. Subsequently, the SPI was calculated for 1 and 12 months, enabling the identification of drought events. The results revealed pronounced spatiotemporal regional variations and complexities in the dry–wet climatic patterns of Inner Mongolia, with significant decreases in precipitation emerging as the primary driver of drought occurrences. Approximately 6% of the entire study period experienced short-term drought, while long-term drought periods ranged from 23% to 38%. Regarding multi-year trends, precipitation exhibited a weak increasing trend, while rain days exhibited a weak decreasing trend. Drought exhibited an alleviating trend, with 92% of stations displaying coefficients > 0 for SPI_Month and over 62% of stations displaying coefficients > 0 for SPI_Year. At the monthly scale, drought was most correlated with light rainfall trends and least correlated with moderate rainfall trends. At the annual scale, drought was relatively highly correlated with moderate and heavy rainfall distributions but poorly correlated with light rainfall. The results suggested that achieving the precise monitoring and mitigation of drought disasters in Inner Mongolia in the future will require a combined analysis of indicators, including agricultural drought, hydrological drought, and socio-economic drought. Such an approach will enable a comprehensive analysis of drought characteristics under different underlying surface conditions in Inner Mongolia. Full article

Paddy fields are important anthropogenic emission sources of methane (CH₄). However, it is not clear how rice root development and rhizosphere soil properties affect CH₄ emissions. Therefore, we selected rice varieties with similar growth periods but different root traits in the local area. We measured CH₄ emission fluxes, cumulative CH₄ emissions, root dry weight, root length, and the dissolved organic carbon (DOC), microbial biomass carbon (MBC), redox potential (Eh), ammonium nitrogen (${\mathrm{N}\mathrm{H}}_4^{+}$–N), and nitrate nitrogen (${\mathrm{N}\mathrm{O}}_3^{-}$–N) contents in rhizosphere soil. Methanogens and methanotrophs are crucial factors influencing CH₄ emissions; thus, their abundance and community composition were also assessed. The result showed that CH₄ fluxes of each rice variety reached the peak at tillering stage and jointing-booting stage. The CH₄ emissions in tillering stage were the largest in each growth period. CH₄ emissions had negative correlations with root length, root dry weight, Eh ${\mathrm{N}\mathrm{O}}_3^{-}$–N, methanotroph abundance, and the pmoA/mcrA ratio, and positive correlations with ${\mathrm{N}\mathrm{H}}_4^{+}$–N, MBC, DOC, and methanogen abundance. Path analysis confirmed methanogens and methanotrophs as direct influences on CH₄ emissions. Root development and rhizosphere soil properties affect CH₄ emissions indirectly through these microbes. This study suggests that choosing rice varieties with good root systems and managing the rhizosphere soil can effectively reduce CH₄ emissions. Full article

The Meteorological Optical Range (MOR) is a measurement of atmospheric visibility. Visibility impairment has been linked to increased aerosol levels in the air. This study conducted statistical analyses using meteorological, air pollutant concentration, and MOR data collected in Mexico City from August 2014 to December 2015 to determine the factors contributing to haze occurrence (periods when MOR < 10,000 m), defined using a light scatter sensor (PWS100). The outcomes revealed seasonal patterns in PM_2.5 and relative humidity (RH) for haze occurrence along the year. PM_2.5 levels during hazy periods in the dry season were higher compared to the wet season, aligning with periods of poor air quality (PM_2.5 > 45 μg/m³). Pollutant-to-CO ratios suggested that secondary aerosols’ production, led by SO₂ conversion to sulfate particles, mainly impacts haze occurrence during the dry season. Meanwhile, during the rainy season, the PWS100 registered haze events even with PM_2.5 values close to 15 μg/m³ (considered good air quality). The broadened distribution of extinction efficiency during the wet period and its correlation with RH suggest that aerosol water vapor uptake significantly impacts visibility during this season. Therefore, attributing poor visibility strictly to poor air quality may not be appropriate for all times and locations. Full article

This research presents an experimental analysis of the mechanical behavior of masonry mortars incorporating disposable face masks (FMs) cut into two different sizes. The objective is to provide experimental data contributing to the consolidation of recycling FMs in mortar mixtures. To achieve this, two types of mixtures were prepared: one with strips of 3 × 3 mm and another with strips of 3 × 10 mm. These FM strips were added in different proportions by the volume of mortar (0%, 0.2%, 0.5%, 0.8%, 1.0%, and 1.5%). In all mortars, the dry bulk density, volume of permeable voids, and water absorption, as well as compressive, flexural, and tensile strengths, were evaluated after a 28-day water immersion curing period. Additionally, two essential properties in masonry mortars were analyzed: air content and shear bond strength. The results indicated that, for both strip sizes, adding FMs up to 0.2% positively affected the flexural and tensile strengths; concerning control mortar, increases of 6% and 1.4%, were recorded, respectively, for the longer strips. At this percentage, the density, air content, and compressive and shear bond strengths are not significantly affected. The results demonstrated that incorporating FMs into mortar mixtures is a promising avenue for sustainable recycling and helps reduce microplastic environmental contamination. Full article

This study investigated the microbiological and parasitic quality of sewage sludge treated in drying beds in Algeria, aiming to contribute to a better understanding of the factors influencing sludge safety for potential agricultural applications in the Algerian context. The research focused on various sludge types (liquid, semi-solid, and solid) and their behavior across different seasons. Standard microbiological methods were employed to quantify total coliforms, fecal streptococci, E. coli, and Clostridium. Controls were implemented to ensure accuracy, with positive controls validating detection methods using known quantities of microorganisms and parasites, while negative controls confirmed the absence of contamination in the testing environment. Parasitic contamination was assessed through microscopic examination for protozoa and helminths. Results revealed substantial variation in microbial concentrations across sludge types and seasons. Liquid sludge, particularly during summer, exhibited the highest levels of total coliforms (up to 7.021 log10) and E. coli (up to 6.049 log10), while solid sludge showed lower counts. Seasonal trends indicated increased microbial levels during warmer months. Parasitic contamination was prevalent in 81% of samples, with protozoan cysts (e.g., Giardia intestinalis and Endolimax nanus) and helminth eggs detected. Despite reducing microbial loads, drying processes alone were insufficient, leaving significant contamination. Enhanced protocols are needed, such as longer drying periods, chemical disinfectants, or advanced technologies like anaerobic digestion or composting. This highlights the need for locally adapted treatment strategies. Furthermore, this research provides specific recommendations for improving sewage sludge management practices in Algeria, taking into account the unique environmental and agricultural context of the country. Full article

The solar drying of sewage sludge in greenhouses is one of the most used solutions in wastewater treatment plants (WWTPs). However, it presents challenges, particularly in terms of efficiency and drying time. In this context, the present study explores the drying performances of an innovative Combined Solar Greenhouse Dryer (CSGD) for sewage sludge. The system integrates rock bed storage (RBS), a solar air collector (SAC), and a solar greenhouse dryer (SGD). A numerical model, developed using TRNSYS software, predicts the drying kinetics of sewage sludge through hourly dynamic simulations based on the climatic conditions of Marrakesh, Morocco. Experimental validation confirmed the accuracy of the model. The results reveal that integrating the SAC with the SGD during the day and the RBS with the SGD at night significantly enhances the drying efficiency of the sewage sludge. During daylight hours, the SAC generates hot air, reaching maximum temperatures of 64 °C in January and 109 °C in July. Concurrently, the outlet air temperature of the RBS rises notably during the day, corresponding to the charging phase of the storage unit. Moreover, during the night, the RBS air temperature exceeds ambient temperatures by approximately 7–16 °C in January and 11–37 °C in July. This integration leads to a substantial reduction in drying time. The reduction in sewage sludge water content from 4 kg/kg of dry solid (20% dry solid content) to 0.24 kg/kg of dry solid (80% dry solid content) is related to a decrease in the drying time from 121 h to 79 h in cold periods and from 47 h to 27 h in warm periods. The drying process is significantly enhanced within the greenhouse, both during daylight and nocturnal periods. The CSGD system proves to be energy-efficient, offering an effective, high-performance solution for sewage sludge management, while also lowering operational costs for WWTPs. This innovative solar drying system combines a thermal storage bed and a solar collector to enhance drying efficiency, even in the absence of sunlight. Full article

Dry socket, also known as alveolar osteitis, presents significant challenges in oral surgery because of severe pain and delayed wound healing. This study aims to address these challenges by developing and evaluating a lidocaine-loaded polyelectrolyte complex thermoresponsive gel (LG) designed to enhance wound healing and provide effective pain management in oral wounds. The thermoresponsive gel transitions from a liquid to a gel at body temperature, ensuring sustained contact with the wound site and prolonged release of lidocaine. The in vitro assessments, including cytotoxicity and wound scratch assays, demonstrated the biocompatibility and therapeutic potential of the LG formulation. Following this, palatal wounds were induced in rats, with healing monitored over a 14-days period. Histological analyses were conducted to assess tissue regeneration and inflammation. The results indicated that the LG formulation significantly improved wound closure rates, reduced inflammation, and accelerated epithelialization compared with control groups, primarily because of the high content of hyaluronic acid (HA). The synergistic effects of HA combined with the thermoresponsive properties of the gel facilitated faster healing. These findings suggest that LG is a promising therapeutic option for enhancing oral wound healing and effectively managing pain, particularly in conditions such as dry socket. Full article

A pilot study was conducted with the aim of adapting the processing of “Kraški pršut”, dry-cured ham, for thighs from heavy pigs. The focus was on the effect of ham weight and salting duration on the quality of dry-cured ham. From a pool of thighs harvested from heavy pigs, a total of 32 green hams were selected (from 16 carcasses) based on weight (two classes; L—lighter, H—heavier) and we used left and right ham for either the standard or a shortened salting phase. Salting duration consisted of phase 1 (7 days for all hams) and phase 2 (7 or 14 days for L, 10 or 17 days for H, in the case of shortened and standard salting, respectively). Equivalent conditions for all hams were maintained during the remaining phases, with a total maturation period of 18 months. The analysis focused on chemical, physical and rheological properties, sensory attributes, and consumer perceptions. The H hams had lower processing losses, resulting in higher moisture and water activity, lower salt content in internal biceps femoris muscle, and a softer texture (instrumental and sensory) than L hams. The salting duration mainly affected weight losses in the salting phase and, consequently, salt content, which was lower in the shortened salting phase, while no effects were observed on texture. The sensory panel perceived weight’s effect on hardness, with L hams being perceived as harder, and salting’s effect on sourness, with hams submitted to longer salting perceived as sourer than H hams. Consumer testing indicated a general preference for softer and less salty hams. Overall, the results show that the applied reduction in salting duration was not substantial, and future trials should explore further optimization in terms of salting and resting phases. Full article

Ultrasonic vibration-assisted grinding (UVAG) has proven to be beneficial for grinding difficult-to-machine materials. This work attempts to enhance the grinding performance of Inconel 718 through a comprehensive study of UVAG characteristics. Grinding experiments were performed in both dry and Minimum Quantity Lubrication (MQL) environments, and assessment of the grinding forces, specific energy, residual stress, and surface topography was done. A substantial reduction of both surface roughness and grinding force components was observed in UVAG compared to conventional grinding (CG). Utilizing UVAG with MQL at the maximum vibration amplitude led to a 64% reduction in tangential grinding force and a 51% decrease in roughness parameter, R_a, when compared to CG conducted in a dry environment. The high-frequency indentations of the abrasives in UVAG generated compressive residual stresses on the ground surface. Surface parameters pointed to uniform texture and SEM images showed widening of abrasive grain tracks on the workpiece surface during UVAG. The utilization of UVAG under MQL produced a synergistic impact and resulted in the lowest grinding forces, specific energy, and optimal surface quality among all the grinding conditions investigated. Overall analysis of the results indicated that the axial configuration of the vibration set-up is favorable for UVAG, and the high-frequency periodic separation-cutting characteristic of the process improves lubricating efficiency and grinding performance. Full article

Sustainable development is one of the main directions of modern agriculture. First of all, sustainability in the agricultural sector can be achieved through the possible abandonment of traditional mineral fertilizers. Many decades of using these fertilizers have led to the degradation of arable soils and to soil and environmental pollution. As a result, this causes reductions in yields and the environmental quality of agricultural products and affects the health of the population. An alternative to traditional mineral fertilizers may be the use of innovative organomineral fertilizers obtained from local resources. These include manure, humus, compost, sediments, etc. In recent years, fertilizers obtained from the sapropels of the bottom sediments of lakes have become widespread. Their distinctive feature is the environmental friendliness and completeness of the content of chemical elements and substances necessary for the development and growth of plants. In addition, the methods of obtaining and applying these fertilizers allow us to talk about their effectiveness in use. The range of applications of these fertilizers is diverse, from use in the form of a dry extract applied directly to the soil to the use of liquid suspensions used at various stages of processing and from pre-sowing seed treatment to watering and spraying plants at different periods of vegetation. Moreover, an important aspect is the research work on the variational use of sapropel fertilizers on different crops, with different methods of production and concentrations and at different stages of processing. This publication contains the results of a study of the effect of the obtained innovative sapropel fertilizer on productivity, wheat grain quality, and economic efficiency (Triticum aestivum L.). To identify the optimal concentration of sapropel extract, laboratory studies were carried out to determine the germination energy and germination of wheat seeds of different varieties when they were soaked in various concentrations: 0.4, 0.8, 1.2, 1.6, and 2.0 g/L. The best indicators of germination energy and germination of wheat seeds during treatment with the extract were obtained at a concentration of 1.2 g/L. The research was conducted at an accredited variety testing laboratory. A field experiment was conducted in the fields of the agrobiological station of North Kazakhstan University named after Manash Kozybayev. The treatment of the seeds was carried out by soaking them in sapropel extract to evenly distribute the substance. The scheme of the field experiment included the option of using foliar treatment with a solution of sapropel extract at the tillering stage. As a result of the application of the obtained extract in the field, environmental and socio-economic efficiency was noted. The conducted field studies note its positive effect and effectiveness on the morphological, qualitative, and quantitative indicators of the wheat harvest. In the areas where wheat seeds were pretreated, as well as where foliar treatment with the resulting sapropel suspension was carried out, the best yield indicators were revealed. In these variants of the experiment with pre-sowing and pre-sowing and foliar treatment with the sapropel extract solution, the yield was 3.63 and 3.81 tons per hectare, respectively. The introduction of sapropel extract at the stage of seed treatment before sowing, as well as foliar processing of wheat at the tillering stage, will increase the efficiency and profitability of the agricultural industry and obtain a synergistic effect in the form of socio-economic efficiency and environmental safety of production. In our opinion, this will contribute to the development of sustainable agriculture and the production of environmental products. Full article

One-month-old lettuce seedlings were planted in the field with and without prior hairy vetch (Vicia villosa L.) incorporation (HV and Fallow, respectively). The periods between hairy vetch incorporation and lettuce planting were 1 day, 8 days and 15 days. The inorganic nitrogen concentration in the soil was higher after hairy vetch incorporation, as well as the nitrogen concentration in the lettuce leaves from the HV plot compared to the Fallow plot, at any planting period. When lettuce seedlings were planted 8 days after hairy vetch incorporation, the leaf dry weight in the HV plots was 11% lower than that in the Fallow plots 4 days after planting; however, leaf growth recovered and the dry weight was 24% higher 12 days after planting, which could be due to enhanced leaf growth as the result of additional accumulated nitrogen provided by the hairy vetch. In the incubation experiment, it was suggested that the inhibitory effect of hairy vetch decomposition was not due to allelopathic substances. Therefore, in the hairy-vetch-incorporated field, growth suppression in the lettuce plants occurred early and continued for a short period after hairy vetch incorporation, but the damage due to the growth suppression effect was minor. More importantly, nitrogen supplied from the hairy vetch promoted lettuce growth after the inhibition period. Full article

A better understanding of climate change impact on dry/wet conditions is crucial for agricultural planning and the use of renewable energy, in terms of sustainable development and preservation of natural resources for future generations. The objective of this study was to investigate the impact of climate change on temporal fluctuations of dry/wet conditions in Serbia on multiple temporal scales through multifractal analysis of the standardized precipitation evapotranspiration index (SPEI). We used the well-known method of multifractal detrended fluctuation analysis (MFDFA), which is suitable for the analysis of scaling properties of nonstationary temporal series. The complexity of the underlying stochastic process was evaluated through the parameters of the multifractal spectrum: position of maximum ${\alpha }_0$ (persistence), spectrum width $W$ (degree of multifractality) and skew parameter $r$ dominance of large/small fluctuations). MFDFA was applied on SPEI time series for the accumulation time scale of 1, 3, 6 and 12 months that were calculated using the high-resolution meteorological gridded dataset E-OBS for the period from 1961 to 2020. The impact of climate change was investigated by comparing two standard climatic periods (1961–1990 and 1991–2020). We found that all the SPEI series show multifractal properties with the dominant contribution of small fluctuations. The short and medium dry/wet conditions described by SPEI-1, SPEI-3, and SPEI-6 are persistent ($0.5<{\alpha }_0<1$); stronger persistence is found at higher accumulation time scales, while the SPEI-12 time series is antipersistent ($0<{\alpha }_0-1<0.5$). The degree of multifractality increases from SPEI-1 to SPEI-6 and decreases for SPEI-12. In the second period, the SPEI-1, SPEI-3, and SPEI-6 series become more persistent with weaker multifractality, indicating that short and medium dry/wet conditions (which are related to soil moisture and crop stress) become easier to predict, while SPEI-12 changed toward a more random regime and stronger multifractality in the eastern and central parts of the country, indicating that long-term dry/wet conditions (related to streamflow, reservoir levels, and groundwater levels) become more difficult for modeling and prediction. These results indicate that the complexity of dry/wet conditions, in this case described by the multifractal properties of the SPEI temporal series, is affected by climate change. Full article