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Search Results (12,369)

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19 pages, 9096 KiB  
Article
Speech Enhancement Based on Unidirectional Interactive Noise Modeling Assistance
by Yuewei Zhang, Huanbin Zou and Jie Zhu
Appl. Sci. 2025, 15(6), 2919; https://doi.org/10.3390/app15062919 - 7 Mar 2025
Viewed by 166
Abstract
It has been demonstrated that interactive speech and noise modeling outperforms traditional speech modeling-only methods for speech enhancement (SE). With a dual-branch topology that simultaneously predicts target speech and noise signals and employs bidirectional information communication between the two branches, the quality of [...] Read more.
It has been demonstrated that interactive speech and noise modeling outperforms traditional speech modeling-only methods for speech enhancement (SE). With a dual-branch topology that simultaneously predicts target speech and noise signals and employs bidirectional information communication between the two branches, the quality of the enhanced speech is significantly improved. However, the dual-branch topology greatly increases the model complexity and deployment cost, thus limiting its practicality. In this paper, we propose UniInterNet, a unidirectional information interaction-based dual-branch network to achieve noise modeling-assisted SE without any increase in complexity. Specifically, the noise branch still receives information from the speech branch to achieve more accurate noise modeling. Subsequently, the noise modeling results are utilized to assist the learning of the speech branch during backpropagation, while the speech branch no longer receives the auxiliary information from the noise branch, so only the speech branch is required during model deployment. Experimental results demonstrate that under the causal inference condition, the performance of UniInterNet only marginally decreases compared to the corresponding bidirectional information interaction scheme, while the model inference complexity is reduced by about 75%. With comparable overall performance, UniInterNet also outperforms previous interactive speech and noise modeling-based benchmarks in terms of causal inference and model complexity. Furthermore, UniInterNet surpasses other existing competitive methods. Full article
(This article belongs to the Section Computing and Artificial Intelligence)
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<p>Overall architecture of unidirectional information interaction-based dual-branch network (UniInterNet).</p>
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<p>(<b>a</b>) The detail of the two-dimensional convolutional (Conv2d) block. (<b>b</b>) The detail of the two-dimensional deconvolutional (DeConv2d) block.</p>
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<p>The diagram of time–frequency sequence modeling (TFSM) block. During temporal sequence modeling, a causal gated recurrent unit (GRU) layer is employed in the speech branch, while a non-causal bidirectional GRU (BiGRU) layer is utilized in the noise branch.</p>
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<p>Structure of the unidirectional interaction module.</p>
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<p>Visualization of the spectrum of the following: (<b>a</b>) noisy speech; (<b>b</b>) clean speech; (<b>c</b>) enhanced speech by SiNet; (<b>d</b>) enhanced speech by BiInterNet; (<b>e</b>) enhanced speech by UniInterNet-CausalNoise; (<b>f</b>) enhanced speech by UniInterNet. The noise type is open area cafeteria noise.</p>
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<p>Visualization of the spectrum of the following: (<b>a</b>) noisy speech; (<b>b</b>) clean speech; (<b>c</b>) enhanced speech by UniInterNet w/o EncInter; (<b>d</b>) enhanced speech by UniInterNet w/o RecInter; (<b>e</b>) enhanced speech by UniInterNet w/o DecInter; (<b>f</b>) enhanced speech by UniInterNet. The noise type is public square noise.</p>
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21 pages, 1835 KiB  
Article
Leaf to Root Morphological and Anatomical Indicators of Drought Resistance in Coffea canephora After Two Stress Cycles
by Guilherme A. R. de Souza, Danilo F. Baroni, Wallace de P. Bernado, Anne R. Santos, Larissa C. de S. Barcellos, Letícia F. T. Barcelos, Laísa Z. Correia, Claudio M. de Almeida, Abraão C. Verdin Filho, Weverton P. Rodrigues, José C. Ramalho, Miroslava Rakočević and Eliemar Campostrini
Agriculture 2025, 15(6), 574; https://doi.org/10.3390/agriculture15060574 - 7 Mar 2025
Viewed by 92
Abstract
Coffea canephora genotypes adopt distinct strategies to cope with drought and rehydration. We hypothesized that the greater drought tolerance of genotype ‘3V’ compared to ‘A1’, previously reflected in physiological and anatomical leaf traits after two water-stress (WS) cycles, could also be observed in [...] Read more.
Coffea canephora genotypes adopt distinct strategies to cope with drought and rehydration. We hypothesized that the greater drought tolerance of genotype ‘3V’ compared to ‘A1’, previously reflected in physiological and anatomical leaf traits after two water-stress (WS) cycles, could also be observed in P–V curve responses, root and branch anatomy, leaf midrib elongation (CVL), and root distribution. The ‘3V’ and ‘A1’ plants were grown under well-watered (WW) conditions and two cycles of water stress (WS). The ‘3V’ was more sensitive to WS, with reduced branch xylem vessel density (BXVD), while ‘A1’ demonstrated increased BXVD. Root xylem vessel area (RXVA) decreased to a greater extent in ‘3V’ than in ‘A1’, and both genotypes showed increased bulk elastic modulus. Regardless of water conditions, ‘A1’ maintained a higher relative leaf water content at the turgor loss point (RWCTLP). Morphological acclimation did not occur in the second WS cycle. The ‘3V’ plants developed greater root mass in deeper soil layers than ‘A1’ under the WS condition. These findings suggest that ‘A1’ follows a conservative drought-avoidance strategy with lower physio-morphological plasticity, while ‘3V’ exhibits greater drought tolerance. Such responses highlighted coordinated physiological, morphological, and anatomical adaptations of the above- and below-ground organs for resource acquisition and conservation under WS. Full article
(This article belongs to the Section Crop Production)
15 pages, 2475 KiB  
Article
Silicon Deposition and Phytolith Morphological Variation in Culm Sheaths of Dendrocalamus brandisii at Different Growth Stages
by Siyuan Duan, Maobiao Li, Dongbo Xie, Rui Xu, Shuguang Wang, Changming Wang and Hui Zhan
Plants 2025, 14(6), 841; https://doi.org/10.3390/plants14060841 - 7 Mar 2025
Viewed by 200
Abstract
Bamboo is an efficient silicon accumulator with diverse phytolith morphotypes and composition. The bamboo culm sheath, traditionally considered as a modified leaf, plays a key role in bamboo taxonomy and provides significant mechanical and physiological support for shoot development, but its silicon deposition [...] Read more.
Bamboo is an efficient silicon accumulator with diverse phytolith morphotypes and composition. The bamboo culm sheath, traditionally considered as a modified leaf, plays a key role in bamboo taxonomy and provides significant mechanical and physiological support for shoot development, but its silicon deposition and phytolith morphological variation remain underexplored. We investigated silicon variation and phytolith morphology in D. brandisii culm sheaths at different growth stages. The results showed that silicon deposition in D. brandisii culm sheaths at different growth stages was comparable to foliage leaves but significantly greater than branches as in previous research. Phytolith concentration in the culm sheath blades of D. brandisii was higher, associated with their greater silicon content than the sheath bodies. Silicon precipitated and phytoliths were produced as the culm sheath matured. Silicon and phytolith contents were significantly greater in upper culm sheath bodies. D. brandisii culm sheaths were characterized by a high proportion of rondel phytoliths, whereas bilobate and bulliform flabellate phytoliths were not observed. Circular and saddle phytoliths accounted for a very low proportion. Stomata phytoliths were abundant in culm sheaths at the shooting stage and increased with sheath maturation, which supported the transpiration, respiration and photosynthesis in culm sheaths of the shoots. Elongate and acute phytoliths were extremely abundant in D. brandisii culm sheaths and increased with sheath maturation, which enhanced the mechanical and protective role of the culm. The phytolith morphotypes in D. brandisii culm sheaths did not demonstrate a specific trend with sheath position. Saddle phytoliths showed insignificant variation in D. brandisii culm sheaths. Full article
(This article belongs to the Section Plant Development and Morphogenesis)
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<p>Culm sheath of <span class="html-italic">Dendrocalamus brandisii</span> (bar = 10 cm).</p>
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<p>The phytolith morphotypes in the <span class="html-italic">D. brandisii</span> culm sheath blades (bars = 10 μm). 1. Saddle. 2. Ruffle top rondel. 3. Two-spiked rondels. 4. Three-spiked rondels. 5. Four-spiked rondels. 6 and 7. Stomata. 8. Acute. 9. Echinate acute. 10 and 11. Acute. 12. Extended acute. 13. Granulate extended acute. 14. Blocky. 15. Echinate blocky. 16. Plicate blocky. 17. Blocky (rectangular). 18. Circular. 19. Entire elongate. 20. Scrobiculate elongate. 21. Echinate elongate. 22. Bulbous elongate. 23. Scrobiculate elongate. 24. Tuberculate elongate.</p>
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<p>The proportion of phytolith morphotypes in the <span class="html-italic">D. brandisii</span> culm sheath blades.</p>
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<p>The phytolith morphotypes in the <span class="html-italic">D. brandisii</span> culm sheath bodies (Bars = 10 μm). 1. Saddle. 2. Ruffle top rondel. 3. Two-spiked rondel. 4. Three-spiked rondel. 5. Echinate acute. 6. Stomata. 7. Extended acute. 8. Blocky. 9. Echinate blocky. 10. Circular. 11. Echinate elongate. 12. Scrobiculate elongate.</p>
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<p>The proportion of phytolith morphotypes in the <span class="html-italic">D. brandisii</span> culm sheath bodies.</p>
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<p>Culm sheath of <span class="html-italic">Dendrocalamus brandisii</span> (bar = 6 cm).</p>
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10 pages, 4363 KiB  
Article
Temperature-Dependent Compensation Points in GdxFe1−x Ferrimagnets
by Chao Chen, Cuixiu Zheng, Shanshan Hu, Jianwei Zhang and Yaowen Liu
Materials 2025, 18(6), 1193; https://doi.org/10.3390/ma18061193 - 7 Mar 2025
Viewed by 102
Abstract
Recent experiments have reported distinct handedness of spin waves across the compensation temperatures of ferrimagnets, offering promising functionalities for ferrimagnet-based magnonic applications with two distinct polarizations. This paper investigates the effects of various factors on the compensation points of GdFe ferrimagnets through atomistic-level [...] Read more.
Recent experiments have reported distinct handedness of spin waves across the compensation temperatures of ferrimagnets, offering promising functionalities for ferrimagnet-based magnonic applications with two distinct polarizations. This paper investigates the effects of various factors on the compensation points of GdFe ferrimagnets through atomistic-level spin dynamics simulations. The results show that as the Gd composition increases, both the magnetization compensation temperature and the angular momentum compensation temperature of the GdFe alloy increase, with a linear relationship observed between the two compensation temperatures. Furthermore, we show that external magnetic fields and antiferromagnetic exchange strength can also modulate the compensation temperatures. Moreover, the antiferromagnetic exchange strength also affects the resonance frequency of ferrimagnetic materials. In the absence of an external field, the resonance frequency of GdFe is divided into two branches and both increase linearly with the increase in antiferromagnetic exchange strength. This study may stimulate fundamental research on compensated ferrimagnets, which may be useful for building chirality-based spintronics. Full article
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<p>(<b>a</b>) Illustration of atomistic simulation model for the Gd<sub>x</sub>Fe<sub>1−x</sub> ferrimagnetic alloy. The magnetic moments of Gd and Fe atoms are antiparallel in the ground state. <span class="html-italic">B</span> is the external magnetic field applied in <span class="html-italic">z</span>-axis direction. (<b>b</b>) The calculated magnetic moments of sublattice Gd (red solid squares with line) and Fe sublattice (blue solid squares with line) as functions of temperature for a given concentration of <span class="html-italic">x</span> = 0.24. The open colored squares with lines show the corresponding angular momenta of the two sublattices. The two crossing points between the curves indicate the magnetization compensation temperature (T<sub>M</sub>) and angular momentum compensation temperature (T<sub>A</sub>), respectively.</p>
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<p>(<b>a</b>) The calculated magnetization compensation temperature T<sub>M</sub> and angular momentum compensation temperature T<sub>A</sub> as functions of different Gd proportions. The inset plot shows the comparison of the T<sub>M</sub> between our simulation and experimental data taken from Ref. [<a href="#B6-materials-18-01193" class="html-bibr">6</a>]. (<b>b</b>) Linear relationship between T<sub>M</sub> and T<sub>A</sub>, fitted by a linear function of T<sub>A</sub> = 0.85TM + C.</p>
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<p>(<b>a</b>) The calculated magnetization compensation temperature (T<sub>M</sub>) and angular momentum compensation temperature (T<sub>A</sub>) as functions of external magnetic field for a fixed concentration of Gd<sub>0.24</sub>Fe<sub>0.76</sub>. (<b>b</b>,<b>c</b>) The observed precession trajectories of the resonance mode at external magnetic fields of 3T and 7T, respectively. The trajectories of Fe and Gd sublattices are projected onto the xy-phase plane. Where red represents the trajectory of Gd sublattices and black represents the trajectory of Fe sublattices.</p>
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<p>The calculated magnetization compensation temperature (T<sub>M</sub>) as a function of sublattice exchange coupling strength J<sub>Fe-Gd</sub> between the Fe and Gd atoms.</p>
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<p>(<b>a</b>) The resonance frequency as a function of sublattice exchange coupling strength J<sub>Fe-Gd</sub> between the Fe and Gd atoms. (<b>b</b>) The magnetization precession trajectory of low-frequency mode when J<sub>Fe-Gd</sub> = 5 meV, with right-handed chirality (<b>c</b>) The magnetization precession trajectory of high-frequency mode when J<sub>Fe-Gd</sub> = 5 meV, with left-handed chirality.</p>
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23 pages, 10348 KiB  
Article
Genome-Wide Identification of the SWEET Gene Family and Functional Analysis of BraSWEET10 in Winter B. rapa (Brassica rapa L.) Under Low-Temperature Stress
by Jinli Yue, Shunjie Yuan, Lijun Liu, Zaoxia Niu, Li Ma, Yuanyuan Pu, Junyan Wu, Yan Fang and Wancang Sun
Int. J. Mol. Sci. 2025, 26(6), 2398; https://doi.org/10.3390/ijms26062398 - 7 Mar 2025
Viewed by 40
Abstract
Sugars will eventually be exported transporter (SWEET), a class of glucose transport proteins, is crucial in plants for glucose transport by redistribution of sugars and regulates growth, development, and stress tolerance. Although the SWEET family has been studied in many plants, little is [...] Read more.
Sugars will eventually be exported transporter (SWEET), a class of glucose transport proteins, is crucial in plants for glucose transport by redistribution of sugars and regulates growth, development, and stress tolerance. Although the SWEET family has been studied in many plants, little is known about its function in winter B. rapa (Brassica rapa L.). Bioinformatics approaches were adopted to identify the SWEET gene (BraSWEETs) family in B. rapa to investigate its role during overwintering. From the whole-genome data, 31 BraSWEET genes were identified. Gene expansion was realized by tandem and fragment duplication, and the 31 genes were classified into four branches by phylogenetic analysis. As indicated by exon–intron structure, cis-acting elements, MEME (Multiple EM for Motif Elicitation) motifs, and protein structure, BraSWEETs were evolutionarily conserved. According to the heat map, 23 BraSWEET genes were differentially expressed during overwintering, revealing their potential functions in response to low-temperature stress and involvement in the overwintering memory-formation mechanism. BraSWEET10 is mainly associated with plant reproductive growth and may be crucial in the formation of overwintering memory in B. rapa. The BraSWEET10 gene was cloned into B. rapa (Longyou-7, L7). The BraSWEET10 protein contained seven transmembrane structural domains. Real-time fluorescence quantitative PCR (qRT-PCR) showed that the BraSWEET10 gene responded to low-temperature stress. BraSWEET10 was localized to the cell membrane. The root length of overexpressing transgenic A. thaliana was significantly higher than that of wild-type (WT) A. thaliana under low temperatures. Our findings suggest that this gene may be important for the adaptation of winter B. rapa to low-temperature stress. Overall, the findings are expected to contribute to understanding the evolutionary links of the BraSWEET family and lay the foundation for future studies on the functional characteristics of BraSWEET genes. Full article
(This article belongs to the Collection Advances in Molecular Plant Sciences)
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<p>The transmembrane domain of BraSWEET proteins. The blue lines signify the intracellular region. The thick purple line denotes the transmembrane region. Yellow lines indicate the extracellular region.</p>
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<p>Gene structure and motifs of the <span class="html-italic">BraSWEET</span> genes. (<b>A</b>) The phylogenetic tree of BraSWEET proteins. (<b>B</b>) The exon–intron structure of 31 <span class="html-italic">BraSWEET</span> genes. Exons and introns are represented by rose boxes and blue lines, respectively. (<b>C</b>) The motif composition of BraSWEET proteins. The seven motifs are represented by differently colored rectangles.</p>
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<p>Phylogenetic tree of SWEET proteins in <span class="html-italic">Brassica rapa</span> L. (L7) and <span class="html-italic">A. thaliana</span>. The numbers on the branches indicate the bootstrap percentage values calculated from 1000 replicates. The genes in the pink, yellow, blue, and green clades are clubbed in Group1, Group2, Group3, and Group4, respectively. The clades containing only <span class="html-italic">AtSWEET</span> genes are marked with a red star. The clade containing only one MtN3 motif is indicated using a green triangle.</p>
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<p>Chromosomal locations of <span class="html-italic">BraSWEET</span> genes. Black lines represent the gene position on the chromosome. Tandemly duplicated genes are indicated with orange boxes.</p>
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<p>Synteny analysis for the SWEET family in <span class="html-italic">B. rapa</span> (L7). Gray lines indicate all synteny blocks in the genome of <span class="html-italic">B. rapa</span> (L7). Red lines indicate the duplication of <span class="html-italic">BraSWEET</span> gene pairs.</p>
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<p>Synteny analysis of SWEET genes in <span class="html-italic">B. rapa</span> (L7), Arabidopsis, and Chinese cabbage. The gray lines in the background represent collinear blocks in genomes of <span class="html-italic">B. rapa</span> (BrapaL7), A. thaliana (ATH), and Chinese cabbage (rapa), and the red lines highlight collinear SWEET gene pairs.</p>
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<p>Predicted tertiary structure of BraSWEET proteins.</p>
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<p>Cis-acting elements in the promoter regions of <span class="html-italic">BraSWEETs</span>. Cis-acting elements were identified by PlantCARE using upstream 1500 bp sequences of the <span class="html-italic">BraSWEETs</span>. Red inverted triangle, green inverted triangle, brown square, blue triangle, light blue square, orange inverted triangle, purple square, dark green square, dark red triangle, and red inverted triangle represent <span class="html-italic">ABRE</span>, <span class="html-italic">ARE</span>, <span class="html-italic">DRE</span>, <span class="html-italic">ERE</span>, <span class="html-italic">LTR</span>, <span class="html-italic">MBS</span>, <span class="html-italic">MYB</span>, <span class="html-italic">MYC</span>, and <span class="html-italic">W-Box</span>, respectively. The scale bar on the bottom indicates the length of promoter sequences.</p>
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<p>Predicted protein–protein interaction network for BraSWEET proteins. The network nodes represent proteins. The line width indicates the reliability of the interaction. The node size represents the number of proteins that interact with each other.</p>
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<p>Expression profiles of 23 <span class="html-italic">BraSWEWTs</span> genes in different overwintering periods. (<b>A</b>) Heat map of <span class="html-italic">BraSWEWTs</span> genes in six periods of overwintering (S1–S6). (<b>B</b>) Plant growth map in different wintering periods (S1–S6).</p>
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<p>Subcellular localization of BraSWEET10 in tobacco. Treatment: 20% sucrose, 5–10 min. (<b>A</b>) Fluorescence image for BraSWEET10-GFP. (<b>B</b>) Bright field. (<b>C</b>) Merger of the first two images.</p>
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<p>Expression level of <span class="html-italic">BraSWEET10</span> in <span class="html-italic">transgenic A. thaliana.</span> WT: wild type, 1#/2#/3#: <span class="html-italic">BraSWEET10</span> transgenic <span class="html-italic">A. thaliana</span>. <sup>a</sup> <span class="html-italic">p</span> &lt; 0.01 vs. WT group, <sup>b</sup> <span class="html-italic">p</span> &lt; 0.05 vs. WT group.</p>
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<p>Root length of transgenic <span class="html-italic">A. thaliana</span> after low-temperature stress. WT: wild type, 3#: <span class="html-italic">BraSWEET10</span> transgenic <span class="html-italic">A. thaliana.</span> (<b>A</b>) Normal condition culture, (<b>B</b>) low-temperature (4 °C) treatment, (<b>C</b>) root length of <span class="html-italic">A. thaliana</span> plants after low-temperature treatment. <sup>a</sup> <span class="html-italic">p</span> &lt; 0.01, 3# group vs. WT group.</p>
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18 pages, 3781 KiB  
Article
Aging Gut-Brain Interactions: Pro-Inflammatory Gut Bacteria Are Elevated in Fecal Samples from Individuals Living with Alzheimer’s Dementia
by Alison I. C. Donaldson, Claire L. Fyfe, Jennifer C. Martin, Ellen E. Smith, Graham W. Horgan, Phyo K. Myint, Alexandra M. Johnstone and Karen P. Scott
Geriatrics 2025, 10(2), 37; https://doi.org/10.3390/geriatrics10020037 - 7 Mar 2025
Viewed by 110
Abstract
Background/Objectives: Alzheimer’s disease (AD) is the most common form of dementia, characterized by an irreversible decline in cognitive function. The pathogenesis of several neurodegenerative disorders has been linked to changes in the gut microbiota, transmitted through the gut-brain axis. Methods: We [...] Read more.
Background/Objectives: Alzheimer’s disease (AD) is the most common form of dementia, characterized by an irreversible decline in cognitive function. The pathogenesis of several neurodegenerative disorders has been linked to changes in the gut microbiota, transmitted through the gut-brain axis. Methods: We set out to establish by case-control study methodology whether there were any differences in the composition and/or function of the gut microbiota between older resident adults in care homes with or without an AD diagnosis via analysis of the microbial composition from fecal samples. Results: The microbial composition, determined by 16S rRNA gene profiling, indicated that AD sufferers had significantly increased proportions of Escherichia/Shigella and Clostridium_sensu_stricto_1, and significantly decreased proportions of Bacteroides, Faecalibacterium, Blautia, and Roseburia species. The increase in potentially pro-inflammatory bacteria was consistent with slightly higher concentrations of calprotectin, a biomarker of gut inflammation. Fecal concentrations of most microbial metabolites measured were similar across groups, although participants with AD had significantly increased proportions of the branched-chain fatty acid, iso-butyrate, and lower overall concentrations of total short chain fatty acids. Conclusions: Participants with Alzheimer’s disease have several key differences within their gut microbiota profile, in contrast to care home residents without Alzheimer’s disease. The altered microbiome included both compositional and functional changes linked to poorer health and gut inflammation. Full article
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<p>Diagram illustrating the hypothesized bidirectional signaling between the gut and the brain, and the potential factors affecting this.</p>
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<p>Percentages of specific fatty acids as proportions of total fatty acids detected in fecal samples from the AGE-GB study cohorts (Controls—Blue; AD—Grey) compared to a healthy adult (yellow). (<b>A</b>) SCFA; (<b>B</b>) BCFA. Healthy adults are values from 78 free-living healthy individuals [<a href="#B41-geriatrics-10-00037" class="html-bibr">41</a>]. Mean values (+/− standard error of the mean) and individual values are plotted. Superscripts indicate significant differences (<span class="html-italic">p</span> &lt; 0.05) between values. Note the <span class="html-italic">y</span>-axis scale is different between panels (<b>A</b>,<b>B</b>).</p>
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<p>Different alpha diversity metrics showing no significant difference in the microbial diveryes tsity between control (blue) and those with AD (grey).</p>
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<p>Principle coordinate analysis to illustrate grouping of samples based on Bray–Curtis dissimilarity showing replicate samples from the same volunteer (same numbers) clustered closely together.</p>
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<p>(<b>A</b>,<b>B</b>) Relative abundance of most abundant taxa showing data for each individual sample on the left and combined study groups C and AD on the right. (<b>A</b>) Phylum level; (<b>B</b>) genus level. The most abundant 17 genera across the entire sample set are shown in order of average abundance (top to bottom).</p>
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<p>Relative abundance of the 23 most prevalent bacterial genera present at &gt;1% of total in Controls (<span class="html-italic">n</span> = 19, Blue), compared to the combined AD groups (<span class="html-italic">n</span> = 24, Grey). Mean values (+/− standard error of the mean) are plotted. Significant differences between groups are indicated by asterisks (*, **, *** correspond to <span class="html-italic">p</span> &lt; 0.05, <span class="html-italic">p</span> &lt; 0.01, <span class="html-italic">p</span> &lt; 0.001 respectively).</p>
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<p>Principal component analysis investigating correlations between bacteria and fecal metabolites revealing the clear separation between the control (blue) and AD cohorts (grey).</p>
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17 pages, 5534 KiB  
Article
The Pole-to-Ground Fault Current Calculation Method and Impact Factor Investigation for Monopole DC Grids
by Liang Chen, Wei Yi, Pan Deng, Shen Ma, Da Kuang and Hongyu Cai
Electronics 2025, 14(6), 1067; https://doi.org/10.3390/electronics14061067 - 7 Mar 2025
Viewed by 57
Abstract
Flexible DC grids are an important technological means for optimizing power supply structures and promoting energy transition. However, as a system with low inertia and weak damping, the flexible DC grid inherently faces challenges, such as rapid rising of fault currents, vulnerability to [...] Read more.
Flexible DC grids are an important technological means for optimizing power supply structures and promoting energy transition. However, as a system with low inertia and weak damping, the flexible DC grid inherently faces challenges, such as rapid rising of fault currents, vulnerability to significant damage, difficulty in fault interruption, and with regard to the poor overcurrent-withstanding capabilities of power electronic devices. To address these issues, this paper proposes a method for calculating the single-pole ground fault current in a symmetrical monopolar DC grid, and further introduces a matrix exponential calculation method. This method enables quantitative analysis of the influence of various component parameters on the fault current, taking into account the dynamic characteristics of both the faulted and healthy poles in the DC system. The results demonstrate the high accuracy of this calculation method. The analysis reveals that the inductance of the faulted branch has the greatest impact on the fault current, while the inductances of the adjacent outgoing lines also have a certain influence. In contrast, the inductances of lines not adjacent to the faulted branch have minimal impacts on the fault current. Furthermore, the grounding electrode parameters of the converter station connected to the faulted branch exert the most significant influence on the fault current, with the grounding electrode parameters of neighboring converter stations also showing a notable effect. This indicates that the fault current is impacted by the topology of the nearby DC grid, but is not affected by the fault currents at remote converter stations. Full article
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<p>The different grounding types for a monopole DC system.</p>
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<p>The detailed and equivalent symmetrical monopole MMC model for analysis.</p>
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<p>DC grid model for pole-to-ground fault analysis.</p>
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<p>Four-terminal symmetrical monopole DC grid model.</p>
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<p>The fault current results comparison for positive converters.</p>
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<p>The fault current results comparison for negative converters.</p>
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<p>The fault currents <span class="html-italic">i</span><sub>10</sub> influenced by line inductances.</p>
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<p>The <span class="html-italic">i</span><sub>10</sub> fault currents, influenced by grounding electrode resistances.</p>
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<p>The <span class="html-italic">i</span><sub>10</sub> fault currents, influenced by grounding electrode inductances.</p>
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<p>The <span class="html-italic">i</span><sub>10</sub> fault currents, influenced by arm resistances in a positive pole in the fault.</p>
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<p>The <span class="html-italic">i</span><sub>10</sub> fault currents, influenced by arm inductances in a positive pole in the fault.</p>
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<p>The <span class="html-italic">i</span><sub>10</sub> fault currents, influenced by arm inductances in negative healthy poles.</p>
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26 pages, 6237 KiB  
Article
Generative AI in Education: Perspectives Through an Academic Lens
by Iulian Întorsureanu, Simona-Vasilica Oprea, Adela Bâra and Dragoș Vespan
Electronics 2025, 14(5), 1053; https://doi.org/10.3390/electronics14051053 - 6 Mar 2025
Viewed by 98
Abstract
In this paper, we investigated the role of generative AI in education in academic publications extracted from Web of Science (3506 records; 2019–2024). The proposed methodology included three main streams: (1) Monthly analysis trends; top-ranking research areas, keywords and universities; frequency of keywords [...] Read more.
In this paper, we investigated the role of generative AI in education in academic publications extracted from Web of Science (3506 records; 2019–2024). The proposed methodology included three main streams: (1) Monthly analysis trends; top-ranking research areas, keywords and universities; frequency of keywords over time; a keyword co-occurrence map; collaboration networks; and a Sankey diagram illustrating the relationship between AI-related terms, publication years and research areas; (2) Sentiment analysis using a custom list of words, VADER and TextBlob; (3) Topic modeling using Latent Dirichlet Allocation (LDA). Terms such as “artificial intelligence” and “generative artificial intelligence” were predominant, but they diverged and evolved over time. By 2024, AI applications had branched into specialized fields, including education and educational research, computer science, engineering, psychology, medical informatics, healthcare sciences, general medicine and surgery. The sentiment analysis reveals a growing optimism in academic publications regarding generative AI in education, with a steady increase in positive sentiment from 2023 to 2024, while maintaining a predominantly neutral tone. Five main topics were derived from AI applications in education, based on an analysis of the most relevant terms extracted by LDA: (1) Gen-AI’s impact in education and research; (2) ChatGPT as a tool for university students and teachers; (3) Large language models (LLMs) and prompting in computing education; (4) Applications of ChatGPT in patient education; (5) ChatGPT’s performance in medical examinations. The research identified several emerging topics: discipline-specific application of LLMs, multimodal gen-AI, personalized learning, AI as a peer or tutor and cross-cultural and multilingual tools aimed at developing culturally relevant educational content and supporting the teaching of lesser-known languages. Further, gamification with generative AI involves designing interactive storytelling and adaptive educational games to enhance engagement and hybrid human–AI classrooms explore co-teaching dynamics, teacher–student relationships and the impact on classroom authority. Full article
(This article belongs to the Special Issue Techniques and Applications in Prompt Engineering and Generative AI)
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<p>Flowchart diagram.</p>
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<p>Number of publications by year (2019–2025) and by month (December 2022–May 2025).</p>
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<p>Top ten research areas and subject categories with the most publications.</p>
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<p>Top ten most frequent keywords.</p>
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<p>Keyword frequency over time for the period January 2023–December 2024.</p>
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<p>Keyword co-occurrence map.</p>
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<p>Sankey diagram mapping keywords, publication year and research area.</p>
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<p>Top ten affiliations by publication count.</p>
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<p>Collaboration network for the top ten institutions.</p>
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<p>Top ten most cited publications.</p>
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<p>Sentiment in publication abstracts over time using the custom method.</p>
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<p>Sentiment in publication abstracts over time using the VADER method.</p>
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<p>Sentiment in publication abstracts over time using the TextBlob method.</p>
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<p>LDA visualization for Topic 1.</p>
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16 pages, 12999 KiB  
Article
One-Pot Synthesis of Amphiphilic Linear and Hyperbranched Polyelectrolytes and Their Stimuli-Responsive Self-Assembly in Aqueous Solutions
by Angelica Maria Gerardos, Aleksander Forys, Barbara Trzebicka and Stergios Pispas
Polymers 2025, 17(5), 701; https://doi.org/10.3390/polym17050701 - 6 Mar 2025
Viewed by 107
Abstract
Stimuli-responsive polymeric nanostructures are compelling vectors for a wide range of application opportunities. The objective we sought was to broaden the array of self-assembling amphiphilic copolymers with stimuli-responsive characteristics by introducing a hydrophilic tunable monomer, (2-dimethylamino)ethyl methacrylate (DMAEMA), together with a hydrophilic one, [...] Read more.
Stimuli-responsive polymeric nanostructures are compelling vectors for a wide range of application opportunities. The objective we sought was to broaden the array of self-assembling amphiphilic copolymers with stimuli-responsive characteristics by introducing a hydrophilic tunable monomer, (2-dimethylamino)ethyl methacrylate (DMAEMA), together with a hydrophilic one, lauryl methacrylate (LMA), within linear and branched copolymer topologies. Size exclusion chromatography was used to evaluate the resultant linear and hyperbranched copolymers’ molecular weight and dispersity, and FT-IR and 1H-NMR spectroscopy techniques were used to delineate their chemical structure. The structural changes in the obtained self-organized supramolecular structures were thoroughly investigated using aqueous media with varying pH and salinity by dynamic light scattering (DLS), fluorescence spectroscopy (FS), and transmission electron microscopy (TEM). The nanoscale assemblies formed by the amphiphiles indicate significant potential for applications within the field of nanotechnology. Full article
(This article belongs to the Special Issue Advances and Applications of Block Copolymers II)
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<p>Synthesis route of hyperbranched H-P(LMA-co-DMAEMA) copolymer.</p>
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<p>SEC curves of the hyperbranched (<b>a</b>) and linear (<b>b</b>) LMA/DMAEMA copolymers.</p>
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<p><sup>1</sup>H-NMR spectrum of P2 in CDCl<sub>3</sub>. Letters above the spectra peaks correspond to the relevant H nuclei shown in the chemical structure of the copolymer in the inset.</p>
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<p>ATR-FTIR spectra of the hyperbranched copolymers (in the solid state).</p>
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<p>DLS size distributions for P1 (<b>a</b>) and P2 (<b>b</b>) linear copolymer aqueous solutions, C<sub>polymer</sub> = 10<sup>−3</sup> g/mL.</p>
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<p>DLS size distributions for H1 (<b>a</b>) and H2 (<b>b</b>) hyperbranched copolymer aqueous solutions.</p>
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<p>Collective DLS data as a function of salinity. (<b>a</b>) P1 and (<b>b</b>) P2 linear copolymers, C<sub>polymer</sub> = 10<sup>−3</sup> g/mL, pH = 7.</p>
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<p>Collective DLS data as a function of salinity. (<b>a</b>) H1 and (<b>b</b>) H2 hyperbranched copolymers, C<sub>polymer</sub> = 10<sup>−3</sup> g/mL, pH = 7.</p>
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<p>I<sub>1</sub>/I<sub>3</sub> vs. polymer concentration plots for CAC determination regarding (<b>a</b>) P1 and (<b>b</b>) P2 copolymer aqueous solutions, C<sub>pyrene</sub> = 1 μM. (Green lines refer to the two tangent lines described in the text).</p>
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<p>I<sub>1</sub>/I<sub>3</sub> vs. polymer concentration plots for CAC determination regarding (<b>a</b>) H1 and (<b>b</b>) H2 copolymer aqueous solutions. (Green lines refer to the two tangent lines described in the text).</p>
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<p>TEM images from (<b>a</b>) P1 and (<b>b</b>) P2 copolymer solutions.</p>
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<p>TEM images from (<b>a</b>) H1 and (<b>b</b>) H2 copolymer solutions.</p>
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21 pages, 4097 KiB  
Article
Biomass Allometries for Urban Trees: A Case Study in Athens, Greece
by Magdalini Dapsopoulou and Dimitris Zianis
Forests 2025, 16(3), 466; https://doi.org/10.3390/f16030466 - 6 Mar 2025
Viewed by 131
Abstract
Urban street trees often exhibit distinct architectural characteristics compared to their counterparts in natural forests. Allometric equations for the stem (MS), branches (MB), and total dry aboveground biomass of urban trees (MT) were developed, [...] Read more.
Urban street trees often exhibit distinct architectural characteristics compared to their counterparts in natural forests. Allometric equations for the stem (MS), branches (MB), and total dry aboveground biomass of urban trees (MT) were developed, based on 52 destructively sampled specimens, belonging to 10 different species, growing in the Municipality of Athens, Greece. Linear, log-linear, and nonlinear regression analyses were applied, and fit statistics were used to select the most appropriate model. The results indicated that diameter at breast height (D1.3) and tree height (H) are needed for accurately predicting MS, while MB may be estimated based on D1.3. To circumvent the caveat of the additivity property for estimating the biomass of different tree component, nonlinear seemingly unrelated regression (NSUR) was implemented. The 95% prediction intervals for MS, MB, and MT efficiently captured the variability of the sampled trees. Finally, the predictions were compared with estimates from i-Tree, the most widely used model suite for urban and rural forestry analysis, and a mean deviation of 134% (ranging from 3% to 520%) was reported. Therefore, in the absence of urban-specific allometries, the obtained empirical models are proposed for estimating biomass in street trees, particularly in cities with Mediterranean-like climatic influences. Full article
(This article belongs to the Special Issue Urban Green Infrastructure and Urban Landscape Ecology)
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<p>Summary scatterplots for <span class="html-italic">M</span><sub>T</sub>, <span class="html-italic">M</span><sub>S</sub>, <span class="html-italic">M</span><sub>B</sub>, <span class="html-italic">D</span><sub>0.30</sub>, <span class="html-italic">D</span><sub>1.30</sub>, <span class="html-italic">D</span><sub>C</sub>, and <span class="html-italic">H</span> across the pooled dataset <span class="html-italic">(M</span><sub>T</sub>: total aboveground dry biomass, <span class="html-italic">M</span><sub>S</sub>: stem dry biomass, <span class="html-italic">M</span><sub>B</sub>: branches dry biomass, <span class="html-italic">D</span><sub>0.30</sub>: tree diameter at a height of 0.30 m above the ground in centimeters (cm), <span class="html-italic">D</span><sub>1.30</sub>: diameter at breast height, at a height of 1.30 m above the ground in centimeters (cm), <span class="html-italic">D</span><sub>C</sub>: the diameter at the base of the crown in centimeters (cm), <span class="html-italic">H</span>: the total height of the tree in meters (m).</p>
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<p>Linear regression statistics from 52 sampled trees across 10 different species, showing observed versus predicted values for (<b>a</b>) stem and (<b>b</b>) branch biomass.</p>
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<p>Expected biomass values (closed circles) for the following tree component: (<b>a</b>) stem biomass (<span class="html-italic">M</span><sub>S</sub>); (<b>b</b>) branch biomass (<span class="html-italic">M</span><sub>B</sub>); (<b>c</b>) total biomass (<span class="html-italic">M</span><sub>T</sub>) along with the associated 95% prediction intervals (grey squares), calculated using Equation (4a,b).</p>
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<p>Predicted biomass values for the following tree component: (<b>a</b>) stem biomass (<span class="html-italic">M</span><sub>S</sub>); (<b>b</b>) branch biomass (<span class="html-italic">M</span><sub>B</sub>); (<b>c</b>) total biomass (<span class="html-italic">M</span><sub>T</sub>), using NSUR approach based on nonlinear regressions for a sample tree (<span class="html-italic">D</span><sub>1.3</sub> = 30.24 cm, <span class="html-italic">H</span> = 4.7 m), highlighting the consistency of the Probability Density Function (PDF) from NSUR estimates (closed circles represent the mean predicted value) and the observed mean values (open circles).</p>
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18 pages, 7968 KiB  
Article
Stages and Evolution of Strike-Slip Faults of the Ultra-Deep-Burial Ordovician Strata in Fuman Oilfield, Tarim Basin: Evidence from U-Pb Geochronology of Siliceous Minerals
by Chao Yao, Zhanfeng Qiao, Xiao Luo, Tianfu Zhang, Bing Li, Shaoying Chang, Zhenyu Zhang and Jiajun Chen
Minerals 2025, 15(3), 270; https://doi.org/10.3390/min15030270 - 6 Mar 2025
Viewed by 212
Abstract
Siliceous minerals with the property of resistance to diagenetic alteration precipitate during the migration of hydrothermal fluids through strike-slip faults and the interaction of these fluids with host rocks during fault activity. Based on petrological analyses and U-Pb dating of siliceous minerals, the [...] Read more.
Siliceous minerals with the property of resistance to diagenetic alteration precipitate during the migration of hydrothermal fluids through strike-slip faults and the interaction of these fluids with host rocks during fault activity. Based on petrological analyses and U-Pb dating of siliceous minerals, the stages of strike-slip faulting of the ultra-deep-burial Ordovician in the Fuman oilfield were subdivided and their evolutionary process was discussed in combination with seismic interpretation. The results reveal the following: (1) the strike-slip faults contain hydrothermal siliceous minerals, including cryptocrystalline silica, crystalline silica, and radial silica. (2) Based on the twelve U-Pb ages of siliceous minerals (ranging from 458 ± 78 Ma to 174 ± 35 Ma) and five U-Pb ages of calcite, the activity of the strike-slip faults was divided into six stages: the Middle Caledonian, Late Caledonian, Early Hercynian, Middle Hercynian, Late Hercynian, and Yanshanian, corresponding to twelve siliceous U-Pb ages ranging from 458 ± 78 Ma to 174 ± 35 Ma, and five calcitic U-Pb ages. The Late Caledonian and Early Hercynian were the main periods of strike-slip fault activity, while the Late Hercynian period marked the final period of the fault system. (3) Later-stage faults inherited and developed from pre-existing faults. Steep linear strike-slip faults formed during the Middle and Late Caledonian movements. During the Late Hercynian and Yanshanian movements, mid-shallow faults, branch faults, and shallow echelon faults developed on the foundation of these linear faults. The methods and results of this study can guide future hydrocarbon exploration in the Fuman oilfield and can be applied to areas with similar tectonic backgrounds. Full article
(This article belongs to the Special Issue Deformation, Diagenesis, and Reservoir in Fault Damage Zone)
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<p>Distribution of the Ordovician strike-slip faults and drilled samples (<b>a</b>), locations of the Fuman oilfield and YAB outcrop (<b>b</b>), location of the Tarim basin (<b>c</b>), and lithological column (<b>d</b>). F<sub>I</sub> and F<sub>II</sub> mentioned in <a href="#minerals-15-00270-f001" class="html-fig">Figure 1</a>a indicate first-order faults and second-order faults, respectively.</p>
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<p>Photo of experimental core samples: (<b>a</b>) GL1, (<b>b</b>) GL2, (<b>c</b>) GL3, (<b>d</b>) GL3-H, (<b>e</b>) YM5, (<b>f</b>) YM6, (<b>g</b>) YM703, (<b>h</b>) MS5, and (<b>i</b>) MS711. Siliceous minerals occurred in fractures with three colors including dark gray (<b>a</b>–<b>e</b>), grayish white (<b>f</b>–<b>i</b>), and colorless (<b>c</b>–<b>e</b>).</p>
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<p>Optical microscope images under plane-polarized light of samples, except for c with cross-polarized light. (<b>a</b>) GL1, grain limestone with grains replaced by cryptocrystalline silica and cemented by microcrystalline silica. (<b>b</b>) GL2, same description as (<b>a</b>). (<b>c</b>) GL3, same description as (<b>a</b>) except for the radial silica filling in the center of siliceous component. (<b>d</b>–<b>f</b>) GL3-H, two stages of siliceous minerals with cryptocrystalline silica on the outside and radial silica at the core. (<b>g</b>) YM5, cryptocrystalline silica replacing grains and becoming cloudy, and chemically homogeneous radial silica. (<b>h</b>) YM6, silica replacing grains along fracture. (<b>i</b>) YM703, cryptocrystalline silica replacing grains and crystalline silica filling in the fractures. Silica cut by late fractures filled with sparry calcite. (<b>j</b>–<b>k</b>) MS5, bioclastic grain and matrix replaced by cryptocrystalline silica, intergranular and dissolved pores filled by crystalline silica, and residual calcite located inside and at the edges of the bioclastic grain. (<b>l</b>) MS711, same description as (<b>a</b>). Red dots in the figure represent siliceous U–Pb dating targets, while blue dots represent calcareous U–Pb dating targets.</p>
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<p>BSE images and chemical analysis points of the siliceous minerals for GL3 (<b>a</b>,<b>b</b>), GL3-H (<b>c</b>–<b>e</b>), and YM5 (<b>f</b>). Red dots correspond to the points in <a href="#minerals-15-00270-t002" class="html-table">Table 2</a>. Black represents fracture or pores, dark gray represents silica, grayish white represents calcite, and white represents pyrite. The picture in the lower-left corners of (<b>a</b>,<b>c</b>,<b>d</b>,<b>f</b>) is the energy spectrum. Square points in (<b>b</b>) are the laser ablation points for U–Pb dating.</p>
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<p>Comparison of δ<sup>30</sup>Si of experimental samples with different geological reservoirs (Revised from Wang et al. [<a href="#B43-minerals-15-00270" class="html-bibr">43</a>]; Zhang et al. [<a href="#B44-minerals-15-00270" class="html-bibr">44</a>]; Deng et al. [<a href="#B45-minerals-15-00270" class="html-bibr">45</a>]; and Savage et al. [<a href="#B46-minerals-15-00270" class="html-bibr">46</a>,<a href="#B47-minerals-15-00270" class="html-bibr">47</a>]).</p>
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<p>U–Pb dating results of silica (red) and calcite (blue) in sampled fractures by LA-MC-ICP-MS. (<b>a</b>–<b>k</b>) Silica U–Pb dating of GL1, GL2, GL3, GL3-H (cryptocrystalline silica), GL3-H (radial silica), YM5, YM6, YM703, MS5 (cryptocrystalline silica of bioclast), MS5 (cryptocrystalline silica of matrix), and MS711, respectively; (<b>l</b>–<b>p</b>) Calcite U–Pb dating of GL2, GL3-H, YM703, MS5, and MS711; more details shown in <a href="#minerals-15-00270-t001" class="html-table">Table 1</a> and <a href="#minerals-15-00270-f003" class="html-fig">Figure 3</a>.</p>
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<p>The petrological characteristics and U–Pb age of siliceous rocks in the siliceous streak, 20 cm away from diabase intrusion of the Penglaiba Formation in the Yong’anba outcrop. (<b>a</b>) Outcrop photo; (<b>b</b>) sample photo of YAB; (<b>c</b>) optical microscope photo of YAB; (<b>d</b>) U–Pb dating result of YAB.</p>
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<p>The developments of strike-slip faults in the Fuman oilfield based on U–Pb dating [<a href="#B31-minerals-15-00270" class="html-bibr">31</a>,<a href="#B51-minerals-15-00270" class="html-bibr">51</a>].</p>
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<p>Seismic profiles and strike-slip fault interpretation of GL3-H well in F<sub>I</sub>5 (<b>a</b>), YM5 well in F<sub>I</sub>7 (<b>b</b>), and MS5 well in F<sub>I</sub>17 (<b>c</b>).</p>
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<p>Seismic profile (<b>a</b>) and evolution process at different periods (<b>b</b>–<b>f</b>) of F<sub>I</sub>17.</p>
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11 pages, 481 KiB  
Communication
Setting the Stage for Branched-Chain Amino Acids Use in Neurological Pathologies: Does a Single Oral Dose Provide Hours of Elevated Systemic Levels?
by Ezek Mathew, Nathan Jones, McKinley Dews, Dominique Neal and Anders Cohen
Diseases 2025, 13(3), 76; https://doi.org/10.3390/diseases13030076 - 6 Mar 2025
Viewed by 139
Abstract
Background: Recent studies have demonstrated that branched-chain amino acids are neuroprotective and neurorestorative. Branched-chain amino acid supplements are now being recommended to be taken before contact sports to reduce concussions. While peaks and troughs in branched-chain amino acids have previously been reported in [...] Read more.
Background: Recent studies have demonstrated that branched-chain amino acids are neuroprotective and neurorestorative. Branched-chain amino acid supplements are now being recommended to be taken before contact sports to reduce concussions. While peaks and troughs in branched-chain amino acids have previously been reported in hospital settings, the metabolism of a single recommended dose of over-the-counter branched-chain amino acids has yet to be elucidated. Methods: We analyzed a patented branched-chain amino acid product to assess its metabolism in 10 healthy adults. Results: Over the defined time points, measured levels of branched-chain amino acids remained significantly elevated when compared to the physiological baseline. The elevations in measured plasma levels indicate that a single oral dose is a viable intake option for increasing levels of branched-chain amino acids. Conclusions: This information can be leveraged to better plan branched-chain amino acid-based treatment doses in order to treat pathologies such as brain injury. Full article
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<p>Graph of BCAA concentration over time, for the amino acids leucine, isoleucine, and valine. The 0 min time point indicates BCAA concentration at baseline. Additionally, BCAA concentration at 30 min post-oral intake and 180 min post-consumption are included. “Norm” refers to the physiological ranges of amino acids. Paired <span class="html-italic">t</span>-tests were used, with Bonferroni correction applied for two comparisons at α = 0.05. This resulted in a threshold for statistical significance of <span class="html-italic">p</span> &lt; 0.025. Note: *** indicates <span class="html-italic">p</span> &lt; 0.001; **** indicates <span class="html-italic">p</span> &lt; 0.0001.</p>
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17 pages, 12823 KiB  
Article
Remote Sensing Small Object Detection Network Based on Multi-Scale Feature Extraction and Information Fusion
by Junsuo Qu, Tong Liu, Zongbing Tang, Yifei Duan, Heng Yao and Jiyuan Hu
Remote Sens. 2025, 17(5), 913; https://doi.org/10.3390/rs17050913 - 5 Mar 2025
Viewed by 223
Abstract
Nowadays, object detection algorithms are widely used in various scenarios. However, there are further small object detection requirements in some special scenarios. Due to the problems related to small objects, such as their less available features, unbalanced samples, higher positioning accuracy requirements, and [...] Read more.
Nowadays, object detection algorithms are widely used in various scenarios. However, there are further small object detection requirements in some special scenarios. Due to the problems related to small objects, such as their less available features, unbalanced samples, higher positioning accuracy requirements, and fewer data sets, a small object detection algorithm is more complex than a general object detection algorithm. The detection effect of the model for small objects is not ideal. Therefore, this paper takes YOLOXs as the benchmark network and enhances the feature information on small objects by improving the network’s structure so as to improve the detection effect of the model for small objects. This specific research is presented as follows: Aiming at the problem of a neck network based on an FPN and its variants being prone to information loss in the feature fusion of non-adjacent layers, this paper proposes a feature fusion and distribution module, which replaces the information transmission path, from deep to shallow, in the neck network of YOLOXs. This method first fuses and extracts the feature layers used by the backbone network for prediction to obtain global feature information containing multiple-size objects. Then, the global feature information is distributed to each prediction branch to ensure that the high-level semantic and fine-grained information are more efficiently integrated so as to help the model effectively learn the discriminative information on small objects and classify them correctly. Finally, after testing on the VisDrone2021 dataset, which corresponds to a standard image size of 1080p (1920 × 1080), the resolution of each image is high and the video frame rate contained in the dataset is usually 30 frames/second (fps), with a high resolution in time, it can be used to detect objects of various sizes and for dynamic object detection tasks. And when we integrated the module into a YOLOXs network (named the FE-YOLO network) with the three improvement points of the feature layer, channel number, and maximum pool, the mAP and APs were increased by 1.0% and 0.8%, respectively. Compared with YOLOV5m, YOLOV7-Tiny, FCOS, and other advanced models, it can obtain the best performance. Full article
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<p>Two common feature fusion diagrams.</p>
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<p>Convolution and deconvolution diagram.</p>
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<p>PANet information fusion diagram.</p>
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<p>The schematic diagram of the improved neck network.</p>
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<p>Schematic diagram of FFDN module.</p>
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<p>Map of mAP0.5 during the training of Fe-YOLO and FFDN-YOLO.</p>
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<p>Comparison between FE-YOLO and FFDN-YOLO models.</p>
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<p>Comparison diagram of model detection and manual labeling.</p>
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<p>Comparison chart of detection results of different models.</p>
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<p>Variation diagram of loss value during model training.</p>
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<p>Dataset test diagram.</p>
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15 pages, 9988 KiB  
Article
Geometry-Aware 3D Hand–Object Pose Estimation Under Occlusion via Hierarchical Feature Decoupling
by Yuting Cai, Huimin Pan, Jiayi Yang, Yichen Liu, Quanli Gao and Xihan Wang
Electronics 2025, 14(5), 1029; https://doi.org/10.3390/electronics14051029 - 5 Mar 2025
Viewed by 100
Abstract
Hand–object occlusion poses a significant challenge in 3D pose estimation. During hand–object interactions, parts of the hand or object are frequently occluded by the other, making it difficult to extract discriminative features for accurate pose estimation. Traditional methods typically extract features for both [...] Read more.
Hand–object occlusion poses a significant challenge in 3D pose estimation. During hand–object interactions, parts of the hand or object are frequently occluded by the other, making it difficult to extract discriminative features for accurate pose estimation. Traditional methods typically extract features for both the hand and object from a single image using a shared backbone network. However, this approach often results in feature contamination, where hand and object features are mixed, especially in occluded regions. To address these issues, we propose a novel 3D hand–object pose estimation framework that explicitly tackles the problem of occlusion through two key innovations. While existing methods rely on a single backbone for feature extraction, our framework introduces a feature decoupling strategy that shares low-level features (using ResNet-50) to capture interaction contexts, while separating high-level features into two independent branches. This design ensures that hand-specific features and object-specific features are processed separately, reducing feature contamination and improving pose estimation accuracy under occlusion. Recognizing the correlation between the hand’s occluded regions and the object’s geometry, we introduce the Hand–Object Cross-Attention Transformer (HOCAT) module. Unlike traditional attention mechanisms that focus solely on feature correlations, the HOCAT leverages the geometric stability of the object as prior knowledge to guide the reconstruction of occluded hand regions. Specifically, the object features (key/value) provide contextual information to enhance the hand features (query), enabling the model to infer the positions of occluded hand joints based on the object’s known structure. This approach significantly improves the model’s ability to handle complex occlusion scenarios. The experimental results demonstrate that our method achieves significant improvements in hand–object pose estimation tasks on publicly available datasets such as HO3D V2 and Dex-YCB. On the HO3D V2 dataset, the PAMPJPE reaches 9.1 mm, the PAMPVPE is 9.0 mm, and the F-score reaches 95.8%. Full article
(This article belongs to the Special Issue Deep Learning for Computer Vision Application)
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<p>Overview of the framework: The framework consists of backbone, HOCAT, and decoders for hand and object. Initially, the RGB image is processed by the backbone, which separates and optimizes the features of the hand and object. Subsequently, the hand features are fused using HOCAT. Finally, the enhanced hand and object features are input into their respective decoders to estimate the pose.</p>
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<p>Heatmap illustration of the feature maps for the hand and object generated by our backbone network.</p>
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<p>Structure of the Hand–Object Cross-Attention Transformer module.</p>
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<p>Dataset image. (<b>a</b>) Image of HO3D V2 dataset; (<b>b</b>) Image of Dex-YCB dataset.</p>
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<p>Qualitative analysis of the hand pose estimation results on HO3D v2. ‘w/o’ represents the removal of a specific module from the model.</p>
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<p>Qualitative analysis of the hand pose estimation of the proposed method on HO3D v2.</p>
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<p>Qualitative analysis of the object pose estimation of the proposed method on HO3D v2.</p>
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19 pages, 9076 KiB  
Article
Functional Study of GbSMXL8-Mediated Strigolactone Signaling Pathway in Regulating Cotton Fiber Elongation and Plant Growth
by Lingyu Chen, Wennuo Xu, Lingyu Zhang, Qin Chen, Yongsheng Cai, Quanjia Chen and Kai Zheng
Int. J. Mol. Sci. 2025, 26(5), 2293; https://doi.org/10.3390/ijms26052293 - 5 Mar 2025
Viewed by 198
Abstract
The novel plant hormone strigolactones (SL) are involved significantly in plant growth and development. Its key members SMXL6, 7, 8 can modulate SL signal reception and response negatively and can regulate plant branching remarkably. There are relatively scarce studies of cotton [...] Read more.
The novel plant hormone strigolactones (SL) are involved significantly in plant growth and development. Its key members SMXL6, 7, 8 can modulate SL signal reception and response negatively and can regulate plant branching remarkably. There are relatively scarce studies of cotton SMXL gene family, and this study was carried out to clarify the role of GbSMXL8 in cotton fiber development. Phylogenetic analysis identified 48 cotton SMXL genes, which were divided into SMXL-I (SMXL 1, 2), SMXL-II (SMXL 3) and SMXL-III (SMXL6, 7, 8) groups. The results of the cis-element analysis indicated that the SMXL gene could respond to hormones and the environment to modulate cotton growth process. A candidate gene GbSMXL8 was screened out based on the expression difference in extreme varieties of Gossypium barbadense. Tissue-specific analysis indicated that GbSMXL8 was mainly expressed in roots, 20D, 25D, and 35D and was involved in SL signaling pathways. In vitro ovule culture experiments showed that exogenous SLs (GR24) could promote the fiber elongation of G. barbadense, and GbSMXL8 expression was increased after GR24 treatment, indicating that GbSMXL8 was specifically responsive to GR24 in regulating fiber growth. GbSMXL8 knockout resulted in creased length and number of epidermal hairs and the length of fiber, indicating the interference role of GbSMXL8 gene with the development of cotton fiber. The GbSMXL8 transgenic plant was detected with a higher chlorophyll content and photosynthetic rate than those of the control plant, producing a direct impact on plant growth, yield, and biomass accumulation. GbSMXL8 gene knockout could increase plant height, accelerate growth rate, and lengthen fiber length. Intervening GbSMXL8 may mediate cotton growth, plant type formation and fiber elongation. In conclusion, the present study uncovers the function of GbSMXL8-mediated SL signal in cotton, providing theoretical insight for future breeding of new cotton varieties. Full article
(This article belongs to the Special Issue Research on Plant Genomics and Breeding: 2nd Edition)
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<p>Phylogenetic tree of SMXL protein in <span class="html-italic">G. arboreum</span>, <span class="html-italic">G. barbadense</span>, <span class="html-italic">G. raymond</span>, <span class="html-italic">G. hirsutum</span>, <span class="html-italic">Arabidopsis thaliana</span>, and <span class="html-italic">Oryza sativa</span> L. Alignment of SMXL amino acid sequences was completed using ClustalW, and the phylogenetic tree was constructed by MEGA 7.0 using adjacent linkage method, with 1000 repeats. Gh, Gb, Ga, Gr, Os, and At represent <span class="html-italic">G. hirsutum</span>, <span class="html-italic">G. barbadense</span>, <span class="html-italic">G. raymond</span>, <span class="html-italic">G. raymond</span>, <span class="html-italic">Oryza sativa</span> L., and <span class="html-italic">Arabidopsis thaliana</span>.</p>
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<p>Genetic structure and conserved domains of SMXL protein in four cotton species.</p>
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<p>Analysis of cis-acting elements of SMXL in <span class="html-italic">G. hirsutum</span>, <span class="html-italic">G. barbadense</span>, <span class="html-italic">G. Raymond</span>, and <span class="html-italic">G. arboreum</span>.</p>
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<p>Expression analysis of SMXL during fiber development of sea island cotton. (<b>A</b>): Transcriptome expression heat map of different varieties of <span class="html-italic">G. barbadense</span>. (<b>B</b>): Relative expression level of <span class="html-italic">GbSMXL8</span> in different tissues of <span class="html-italic">G. barbadense</span>.</p>
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<p>Positive regulation of polygalactone on the fiber development of <span class="html-italic">G. barbadense</span>. (<b>A</b>): Cotton fiber phenotype (collected at 1 DPA) cultured in vitro for 5, 10, 15, 20, 25, 30 days in a medium containing 15 μM SL synthetic analog GR24, 15 μM SL biosynthesis inhibitor Tis 108, and controls; (<b>B</b>): Average fiber length; (<b>C</b>): SL epi-5DS content in ovules at varied growth stages; (<b>D</b>): relative expression of GbSMXL8 in cotton fibers treated with 15 μM mGR24 or 15 μM Tis 108 for 5, 10, 15, 20, 25, and 30 days; (<b>E</b>): total fresh weight of ovule; (<b>F</b>): total ovule dry weight. * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01; *** <span class="html-italic">p</span> &lt; 0.001; **** <span class="html-italic">p</span> &lt; 0.0001. Wild type (A130768).</p>
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<p>Gene editing SMXL8 target analysis and identification. (<b>A</b>): Cloning of double target sgRNA of <span class="html-italic">GbSMXL8</span> gene; (<b>B</b>): <span class="html-italic">GbSMXL8</span> gene; (<b>C</b>): PCR product of double target sgRNA Agrobacterium; (<b>D</b>): PCR product identification of transgenic plant; (<b>E</b>): Two sgRNA positions of <span class="html-italic">GbSMXL8</span> (<b>F</b>): Sequencing of SmXL8 edited plant.</p>
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<p><span class="html-italic">GbSMXL8</span> inhibited stem epidermal hair growth (<b>A</b>): <span class="html-italic">GbSMXL8</span> edited cotton stem and leaf margin epidermal hair; (<b>B</b>): <span class="html-italic">GbSMXL8</span> edited cotton epidermal hair average length; (<b>C</b>): <span class="html-italic">GbSMXL8</span> edited cotton epidermal hair number. * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01;. WT (ZM 49).</p>
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<p>Phenotypic and physiological characterization of transgenic cotton plants. (<b>A</b>) Net photosynthetic rate (Pn) in leaves of wild-type (WT) and transgenic cotton lines. (<b>B</b>) Stomatal conductance (Gs) in leaves of WT and transgenic lines. (<b>C</b>) Transpiration rate (Tr) in leaves of WT and transgenic lines. (<b>D</b>) Intercellular CO<sub>2</sub> concentration (Ci) in leaves of WT and transgenic lines. (<b>E</b>) Chlorophyll content in leaves of WT and transgenic lines. Notes:WT: Wild-type control (cultivar ZM 49). Statistical significance: * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01; *** <span class="html-italic">p</span> &lt; 0.001 (Student’s <span class="html-italic">t</span>-test). All data represent mean ± SD (<span class="html-italic">n</span> = 3 biological replicates).</p>
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<p>Effect of transgenic plants on fiber development. (<b>A</b>): WT, smxl8 transgenic plant fibers; (<b>B</b>): smxl8 transgenic cotton leaf mature fiber length. * <span class="html-italic">p</span> &lt; 0.05; WT (ZM 49).</p>
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