Search Results (4,213)

Heat stress can disrupt the balance between the heat poultry release into the environment and the heat they generate. Pequi oil has antioxidant properties, which may mitigate the heat stress effects. This study aimed to investigate the response of laying hens to pequi oil supplementation under heat stress using a proteomic approach. A total of 96 Lohmann White laying hens with 26 weeks old were housed in a completely randomized design with a 2 × 2 factorial arrangement. They were housed in two climate chambers, thermal comfort temperature ± 24.04 °C with the relative humidity ± 66.35 and heat stress (HS) ± 31.26 °C with the relative humidity ± 60.62. They were fed two diets: a control diet (CON), basal diet (BD) without additives, and with Pequi oil (PO), BD + 0.6% PO. After 84 days, plasma samples were analyzed using Shotgun and LC-MS/MS. Proteins related to anti-inflammation, transport, and the immune system were differentially expressed in hens fed PO and CON under heat stress compared to those in thermoneutral environments. This helps protect against oxidative stress and may support the body’s ability to manage heat-induced damage, stabilizing protein expression under stress conditions. The ovotransferrin proteins, fibrinogen isoforms, apolipoprotein A-I, Proteasome activator subunit 4, Transthyretin, and the enzyme serine Peptidase Inhibitor_Kazal Type 5, which presented Upregulated (Up) equal to 1, present characteristics that may be crucial for enhancing the adaptive responses of hens to thermal stress, thereby increasing their tolerance and minimizing the negative effects of heat on egg production. The data presented in this manuscript provides new insights into the plasma proteome alterations of laying hens fed a diet supplemented with pequi oil during heat stress challenges. Full article

In recent years, skin-mounted devices have gained prominence in personal wellness and remote patient care. However, the rigid components of many wearables often cause discomfort due to their mechanical mismatch with the skin. To address this, we extend the use of the solderable stretchable sensing system (S4) to develop a wireless skin temperature-sensing smart adhesive. This work introduces two novel types of progress in wearables: the first demonstration of Bluetooth-integration and development of a thin-film-based stretchable inverted-F antenna (SIFA). Characterized through RF simulations, vector network analysis under deformation, and anechoic chamber tests, SIFA demonstrated potential as a low-profile, on-body Bluetooth antenna with a resonant frequency of 2.45 GHz that helps S4 retain its thin overall profile. The final S4 system achieved high correlation (R = 0.95, p < 0.001, mean standard error = 0.04 °C) with commercial sensors during daily activities. These findings suggest that S4-based smart adhesives integrated with SIFAs could offer a promising platform for comfortable, efficient, and functional skin-integrated wearables, supporting a range of health monitoring applications. Full article

Priestia megaterium (P. megaterium PH3) is an endophytic bacterium isolated from peanuts. It has natural resveratrol production ability and shows potential application value. This study analyzed its genetic function and metabolic mechanism through whole-genome sequencing and found that the genome size is 5,960,365 bp, the GC content is 37.62%, and 6132 genes are annotated. Functional analysis showed that this strain contained 149 carbohydrate active enzyme genes, 7 secondary metabolite synthesis gene clusters, 509 virulence genes, and 273 drug-resistance genes. At the same time, this strain has the ability to regulate salt stress, low temperature, and hypoxia. Genomic analysis reveals a stilbene-synthase-containing type III polyketide synthase gene cluster that contributes to resveratrol synthesis. A safety assessment showed that the strain is non-hemolytic, does not produce amino acid decarboxylase, and is not resistant to multiple antibiotics. In the mouse model, P. megaterium PH3 did not have significant effects on body weight, behavior, or physiological indicators. These results provide important basic data and theoretical support for its industrial application and the research and development of plant protection agents. Full article

Refractory superalloys (RSAs) are promising candidates for high-temperature, high-strength applications. Two-phase RSAs containing body-centered cubic (BCC) and ordered B2 phases are among the more promising candidates. Systems containing Ru-based B2 precipitates exhibit stable two-phase microstructures at temperatures in excess of 1600 °C. The present study experimentally investigated one potential foundational ternary system for these alloys, Nb-Ti-Ru. Two alloys, (Nb₃Ti)_0.85Ru_0.15 and (Nb₄Ti)_0.85Ru_0.15, were studied to determine phase equilibria and properties at temperatures between 900 °C and 1300 °C. The B2 phase was found to be dominated by RuTi ordering, although considerable Nb solubility was observed up to 18 mol %. The Nb-rich BCC matrix contained up to 15 mol % Ru and 20 mol % Ti. Although a two-phase microstructure of B2 precipitates in a BCC matrix was confirmed, the distribution of elements in the two phases resulted in a larger lattice misfit than expected. The results obtained in this investigation provide valuable information for the future development of RSAs utilizing Ru-based B2 strengthening precipitates. Full article

This study evaluated the productive performance and carcass traits of broiler chickens during the warm season in dry tropical conditions. Two hundred, 1-day-old chicks were used. Birds were fed on reduced (RED) and standard (STD) diets, and two essential oils (EOs) levels, 0 and 200 ppm. The RED diets were formulated with 10% less energy and 10% less protein than STD diets. In the starter phase, weight gain was greater (p < 0.01) in birds fed STD than birds fed RED. In the starter phase, productive variables were not affected (p ≥ 0.14) by EOs. In the finisher phase, greater feed intake (p = 0.02) and higher weight gain (p = 0.04) were observed in chickens on STD. Feed conversion ratio was similar (p = 0.97) for STD and RED. Throughout this study (1–42 d), greater feed intake (p = 0.02) and higher weight gain (p < 0.01) were found in chickens on STD. Feed conversion ratio was similar (p = 0.51) for STD and RED. Broiler chickens on EO-supplemented diets had better feed conversion (p ≥ 0.08). Hot carcass weight was greater (p < 0.01) in birds on STD. Greater yields of leg-thigh (p = 0.01), back (p = 0.01), and wings (p < 0.01) were observed in RED. Carcass yields, breast yield, pH, and meat temperature were not influenced (p ≥ 0.14) by nutrient concentrations in the diet. Carcass evaluations were not affected (p ≥ 0.11) by EOs. Body temperature was lower (p = 0.03) in birds receiving RED, however EOs had no effect (p ≥ 0.22) on body temperature. For the length of the study, at 20:00 h, broiler chickens fed on RED diets showed lower (p = 0.04) body temperatures. In conclusion, broiler chickens were under heat stress during the study, and body temperatures were reduced in chickens fed on RED diets or with EOs. Diet modification or EO-supplementation may improve feed conversion, though RED diets may have a negative effect on weight gain or carcass values. Full article

Hydroxyapatite (HAP) displays a high degree of similarity to the inorganic components that make up roughly 70% of human hard tissue, and it possesses exceptional biological activity and biocompatibility. It is currently internationally recognized as the most biologically active hard tissue implant material. However, its substandard mechanical properties have significantly limited the application of HAP in areas requiring load bearing or in the repair of large bone defects. In this study, HAP/45S5 bioglass laminated ceramic composites were consolidated using the spark plasma sintering (SPS) technique. The grain growth and phase transformation of HAP and 45S5 bioglass were examined at various sintering temperatures. The mechanical properties of the laminated composites were investigated. At 950 °C, the flexural strength and fracture work of the sintered body were (153.22 ± 7.7) MPa and (2049 ± 34) J·m⁻², respectively. These results corresponded to the load–displacement curves and showed that the composites met the mechanical performance requirements of the support material. Full article

Thermal stress is a health and welfare concern in the poultry industry. Poultry have specific thermoregulation strategies for heat stress (i.e., vasodilatation) or cold stress (i.e., vasoconstriction). Infrared thermal (IRT) analysis is a non-invasive temperature assessment technology with significant benefits compared to conventional temperature measurements, which are invasive and time-consuming. However, a wide range of IRT methodologies and equipment are used for temperature assessment in poultry. The aim of this study was to perform a systematic review and meta-analysis of IRT applications in poultry undergoing thermal stress. The bibliographic search yielded 17 records for qualitative synthesis and 10 for quantitative analysis. The results showed IRT is more commonly studied during heat stress than cold stress, and more research is being conducted on laying hens than other poultry species. Also, four body areas (parts of the head, body, face, and leg) were identified as common areas of interest for body surface temperature measurement. There is a clear thermoregulation response to thermal stress in poultry, with marked differences between featherless and feather-covered areas. IRT in poultry undergoing thermal stress has a good diagnostic value and represents an important welfare assessment tool for future research, particularly when combined with other welfare assessment methods. Full article

Temperature detection is particularly important in the medical and scientific fields. Although there are various temperature detection methods, most of them focus on broad temperature detection, and basic research in specific fields, especially the detection of subtle temperature changes (32–34 °C) during wound infection, is still insufficient. For this purpose, a novel colorimetric temperature sensing probe is designed in this paper, which can quickly and intuitively respond to small temperature changes within a specific range through color changes. In this paper, hyperbranched polyethyleneimine (HPEI) was modified by isobutyrylation to prepare hyperbranched temperature-sensitive polymer (HPEI-IBAm). And it was combined with gold nanoparticles (AuNPs) prepared by a sodium citrate reduction method to construct an HPEI-IBAm-AuNP colorimetric probe. The probe exhibits excellent stability, even at salt concentrations of up to 12 g/L, thanks to the abundant amino functional groups and the large steric hindrance effect unique to HPEI-IBAm. In particular, the temperature detection range of the probe is precisely locked within 32–34 °C, enabling it to respond quickly and accurately to small temperature changes of only 2 °C. This feature is perfectly suited to the practical needs of temperature detection in infected wounds. The linear fitting coefficient of the temperature response is as high as 0.9929, ensuring the accuracy of the test results. The detection performance of the probe remained highly consistent over 10 cycles, fully proving its excellent reusability and durability. In addition, a flexible colorimetric sensor was prepared by combining the probe with polydimethylsiloxane (PDMS) film. This sensor is capable of rapidly detecting human skin temperature in real time, achieving an accuracy of 99.07% to 100.61%. It can provide a possible solution to the challenges of delayed and difficult temperature detection caused by different body parts and uneven surfaces, among others. This demonstrates its extensive practical value and potential, and it is expected to be further applied in the monitoring of wound infections. Full article

Moringa oleifera is renowned for its high antioxidant activity. However, few studies have been conducted on its effects on aquatic animals. The aim of this experiment was to investigate the optimal fermentation process of M. oleifera leaves and to evaluate the effects of fermented M. oleifera leaves on crayfish (9.11 ± 0.3 g) in terms of growth performance, antioxidant capacity, and gut microbiological parameters. By optimizing the fermenting material/water ratio, fermentation time, temperature, and strain, the optimal fermentation conditions of a 10% water ratio + 48 h + 30 °C + inoculation with 2% B. amyloliquefaciens (10⁷ CFU mL⁻¹) were obtained. These conditions resulted in notable increases in the contents of the total protein, total phenols, flavonoids, and amino acids (p < 0.05) while also leading to a notable decrease in the content of tannins in contrast to those of unfermented M. oleifera leaves (p < 0.05). The fermented M. oleifera (FMO) leaves were incorporated at five concentrations, including 0% (control (CT)), 0.25% (0.25FMO), 0.5% (0.5FMO), 1% (1FMO), and 2% (2FMO). The results showed that the 1FMO group performed better in terms of the final body weight (FBW), weight gain rate (WGR), and specific weight gain rate (SGR) compared with the CT group (p < 0.05). In addition, amylase and lipase activities were significantly higher in the 1FMO and 2FMO groups compared with the other groups (p < 0.05). The fermented M. oleifera leaves significantly increased the catalase (CAT) activity in the crayfish (p < 0.05). The superoxide dismutase (SOD) activity was significantly increased in the 0.25FMO, 1FMO, and 2FMO groups, and the malondialdehyde (MDA) content was significantly decreased while the glutathione peroxidase (GSH-PX) content was significantly increased in the 0.5FMO, 1FMO, and 2FMO groups (p < 0.05). Furthermore, the 1FMO group was observed to significantly increase the abundance of Firmicutes while simultaneously reducing the abundance of Aeromonas (p < 0.05) and adjusting the structure of the intestinal microbiome. In conclusion, this study established the optimal fermentation conditions for M. oleifera and obtained a product with high nutrient and low tannin contents. Furthermore, the incorporation of 1% FMO was demonstrated to facilitate growth, enhance the antioxidant capacity, and optimize the gut microbiology in crayfish. Full article

Background/Objectives: Hypothermia can affect multiple organ systems and reduce patient comfort and is an independent predictor of mortality in trauma patients. Early prevention and management prehospitally is critical and can be approached by “warming” patients using active and/or passive measures. Therefore, this systematic review aimed to determine the efficacy of prehospital active warming (AW) in trauma patients. Methods: MEDLINE, Embase, Web of Science and CENTRAL were searched up to June 2024 for studies meeting our inclusion and exclusion criteria. Six studies were identified: four randomised controlled trials (RCTs), a non-randomised trial and an observational study. A risk-of-bias assessment was conducted using either the Cochrane Risk of Bias 2 tool or the ROBINS-I tool. Results: For the primary outcome of end core body temperature (CBT), our meta-analysis calculated a statistically significant mean difference of 0.62 °C ([95%CI: 0.17, 1.07], p = 0.007), favouring AW. For the secondary outcomes of end heart rate and end systolic blood pressure, the mean differences favoured the AW and control groups, respectively, but were not statistically significant (p = 0.45 and p = 0.64). Two of the four RCTs had an overall moderate to high risk of bias, whilst the two observational studies had a high risk of bias. Conclusions: Overall, our results suggest that AW may be effective at managing CBT, but our results are limited by a small sample size, a serious/high overall risk of bias and variable study characteristics. Larger, high-quality studies are needed to inform clinical practice and guidelines. Full article

The suspension system of a commercial vehicle cab plays a crucial role in enhancing ride comfort by mitigating vibrations. However, conventional rubber suspension systems have relatively fixed stiffness and damping properties, rendering them inflexible to load variations and resulting in suboptimal ride comfort under extreme road conditions. Shape memory alloys (SMAs) represent an innovative class of intelligent materials characterized by superelasticity, shape memory effects, and high damping properties. Recent advancements in materials science and engineering technology have focused on rubber-based SMA composite dampers due to their adjustable stiffness and damping through temperature or strain rate. This paper investigates how various structural parameters affect the stiffness and damping characteristics of sleeve-type rubber-based SMA composite vibration dampers. We developed a six-degree-of-freedom vibration differential equation and an Adams multi-body dynamics model for the rubber-based SMA suspension system in commercial vehicle cabins. We validated the model’s reliability through theoretical analysis and simulation comparisons. To achieve a 45% increase in stiffness and a 64.5% increase in damping, we optimized the suspension system’s z-axis stiffness and damping parameters under different operating conditions. This optimization aimed to minimize the z-axis vibration acceleration at the driver’s seat. We employed response surface methodology to design the composite shock absorber structure and then conducted a comparative analysis of the vibration reduction performance of the optimized front and rear suspension systems. This study provides significant theoretical foundations and practical guidelines for enhancing the performance of commercial vehicle cab suspension systems. Full article

Background/Objectives: Global warming and the rise in the average temperature in recent years have increased the risk of heat stroke and also deteriorated performance among athletes. Kendo, a traditional Japanese martial art and also a competitive sport, is reported to have high incidents of heat stroke and related mortality. However, there is no heat management guideline for this specific sport to date and research on its heat management practices and risk factors for heat stroke are limited. The present study conducted a scoping review on studies focused on heat stress and the heat management practices of Kendo players. Methods: A literature search was conducted using five databases (PubMed, SCOPUS, Ichu-shi Web, CiNii, and Google Scholar) and also manually from the references of searched articles. Results: Overall, the studies that have investigated the heat stress of Kendo players are scarce and outdated. Of the 15 references that met screening criteria, 11 studies were conducted in a field setting. The vast majority were conducted on male university students and there was a lack of research on females and on different age groups. Common measures of heat management practices used in previous studies were weight changes before and after training (n = 14), body temperature (n = 9), and heart rate (n = 8). Only a few studies used multiple measures to determine heat stress. Conclusions: Considering the continuation of global warming and the increasing risk of heat stroke, further investigation on heat stress, its association with health and performance, and current heat management practices in Kendo players are warranted. Full article

The resilience and resistance of vegetation are important indicators of the vegetation’s response to droughts. Owing to the uniqueness of the environment in humid karst areas, results from studies on other climatic zones may not necessarily present the status of vegetation resilience and resistance in humid karst areas. Herein, We calculated vegetation resilience and resistance by autoregressive modeling using Enhanced Vegetation Index (EVI), Total Water Storage Anomaly (TWSA), temperature (TA), precipitation (PRE) data, An analysis of variance (ANOVA) was then conducted to compare the differences in resilience and resistance of different vegetation types in the study area, as well as the differences in resilience and resistance of vegetation in different sub-geomorphic zones. Finally, natural factors affecting vegetation resilience and resistance were quantified using partial least squares structural equation modeling (PLS-SEM). The results demonstrate the following points. First, vegetation resilience, total-water-storage anomaly resistance, and vegetation resistance against precipitation anomalies were lower in karst areas of the study area than in non-karst areas of the study area (except for vegetation resistance against temperature anomalies). Second, vegetation resilience was the lowest in some sub-geomorphic zones within karst areas, and it was still comparable to that in semiarid areas. Third, precipitation and temperature were important factors that affected the resilience and resistance of vegetation in karst areas, and the geochemical indicators (CaO, MgO, and SiO₂) of soil parent material were major factors that affected the resistance and resilience of vegetation in non-karst areas. In summary, this study was undertaken to reveal the natural characteristics of vegetation resilience and resistance in humid karst regions. Our findings complement and expand the existing body of knowledge on vegetation resilience and resistance in other ecologically fragile zones limited by moisture. Full article

Heat stress can impair organismal growth by inducing ubiquitination, proteasome-mediated degradation, and subsequent cellular damage. Vitamin C (VC) has been shown to potentially mitigate the detrimental effects of abiotic stresses on cells. Nevertheless, the impact of heat stress on growth plate chondrocytes remains unclear, and the underlying protective mechanisms of VC in these cells warrant further investigation. In this study, we focused on pig thoracic vertebral chondrocytes (PTVCs) that are crucial for promoting the body’s longitudinal elongation and treated them with 41 °C heat stress for 24 h, under varying concentrations of VC. Our findings reveal that, while oxidative stress induced by heat triggers apoptosis and inhibits the ubiquitin-mediated proteolysis pathway, the addition of VC alleviates heat-stress-induced oxidative stress and apoptosis, mitigates cell cycle arrest, and promotes cellular viability. Furthermore, we demonstrate that VC enhances the ubiquitin-proteasome proteolysis pathway by promoting the expression of ubiquitin protein ligase E3A, which thereby stabilizes the ubiquitin-mediated degradation machinery, alleviates the apoptosis, and enhances cell proliferation. Our results suggest the involvement of the ubiquitin-mediated proteolysis pathway in the effects of VC on PTVCs under heat stress, and offer a potential strategy to make use of VC to ensure the skeletal growth of animals under high temperature pressures in summer or in tropical regions. Full article

The biological products used in orthopedics include musculoskeletal allografts—such as bones, tendons, ligaments, and cartilage—as well as biological therapies. Musculoskeletal allografts support the body’s healing process by utilizing preserved and sterilized donor tissue. These allografts are becoming increasingly common in surgical practice, allowing patients to avoid more invasive procedures and the risks associated with donor site morbidity. Bone grafting is one of the most frequently used procedures in orthopedics and traumatology. Biologic approaches aim to improve clinical outcomes by enhancing the body’s natural healing capacity and reducing inflammation. They serve as an alternative to surgical interventions. While preliminary results from animal studies and small-scale clinical trials have been promising, the field of biologics still lacks robust clinical evidence supporting their efficacy. Biological therapies include PRP (platelet-rich plasma), mesenchymal stem cells (MSCs)/stromal cells/progenitor cells, bone marrow stem/stromal cells (BMSCs), adipose stem/stromal cells/progenitor cells (ASCs), cord blood (CB), and extracellular vesicles (EVs), including exosomes. The proper preservation and storage of these cellular therapies are essential for future use. Preservation techniques include cryopreservation, vitrification, lyophilization, and the use of cryoprotective agents (CPAs). The most commonly used CPA is DMSO (dimethyl sulfoxide). The highest success rates and post-thaw viability have been achieved by preserving PRP with a rate-controlled freezer using 6% DMSO and storing other cellular treatments using a rate-controlled freezer with 5% or 10% DMSO as the CPA. Extracellular vesicles (EVs) have shown the best results when lyophilized with 50 mM or 4% trehalose to prevent aggregation and stored at room temperature. Full article