Nothing Special   »   [go: up one dir, main page]

You seem to have javascript disabled. Please note that many of the page functionalities won't work as expected without javascript enabled.
 
 
Sign in to use this feature.

Years

Between: -

Subjects

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Journals

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Article Types

Countries / Regions

remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline
remove_circle_outline

Search Results (2,742)

Search Parameters:
Keywords = ablation study

Order results
Result details
Results per page
Select all
Export citation of selected articles as:
22 pages, 3402 KiB  
Article
Enhancing Wheat Spike Counting and Disease Detection Using a Probability Density Attention Mechanism in Deep Learning Models for Precision Agriculture
by Ruiheng Li, Wenjie Hong, Ruiming Wu, Yan Wang, Xiaohan Wu, Zhongtian Shi, Yifei Xu, Zixu Han and Chunli Lv
Plants 2024, 13(24), 3462; https://doi.org/10.3390/plants13243462 (registering DOI) - 11 Dec 2024
Abstract
This study aims to improve the precision of wheat spike counting and disease detection, exploring the application of deep learning in the agricultural sector. Addressing the shortcomings of traditional detection methods, we propose an advanced feature extraction strategy and a model based on [...] Read more.
This study aims to improve the precision of wheat spike counting and disease detection, exploring the application of deep learning in the agricultural sector. Addressing the shortcomings of traditional detection methods, we propose an advanced feature extraction strategy and a model based on the probability density attention mechanism, designed to more effectively handle feature extraction in complex backgrounds and dense areas. Through comparative experiments with various advanced models, we comprehensively evaluate the performance of our model. In the disease detection task, our model performs excellently, achieving a precision of 0.93, a recall of 0.89, an accuracy of 0.91, and an mAP of 0.90. By introducing the density loss function, we are able to effectively improve the detection accuracy when dealing with high-density regions. In the wheat spike counting task, the model similarly demonstrates a strong performance, with a precision of 0.91, a recall of 0.88, an accuracy of 0.90, and an mAP of 0.90, further validating its effectiveness. Furthermore, this paper also conducts ablation experiments on different loss functions. The results of this research provide a new method for wheat spike counting and disease detection, fully reflecting the application value of deep learning in precision agriculture. By combining the probability density attention mechanism and the density loss function, the proposed model significantly improves the detection accuracy and efficiency, offering important references for future related research. Full article
Show Figures

Figure 1

Figure 1
<p>Dataset samples. (<b>a</b>) is loose smut, (<b>b</b>) is fusarium head blight, (<b>c</b>) is powdery mildew, (<b>d</b>) is leaf spot, (<b>e</b>) is glume blotch, (<b>f</b>) is rust.</p>
Full article ">Figure 2
<p>Dataset augmentation. (<b>a</b>) is CutOut, (<b>b</b>) is CutMix, (<b>c</b>) is Mosaic.</p>
Full article ">Figure 3
<p>The diagram illustrates the workflow of an object detection task using a Transformer model. Starting from the input image, it details the three main stages: data preprocessing, encoder processing, and decoder processing.</p>
Full article ">Figure 4
<p>The diagram shows the detailed design of the object detection network structure. It starts with feature processing, extracts and aligns feature maps through the ROI Align module, and then processes them in different feature channels, including the setup of fully connected layers. N Boxes: refers to the number of proposed regions or bounding boxes in an image; <math display="inline"><semantics> <msub> <mi>x</mi> <mi>i</mi> </msub> </semantics></math>: refers to elements in weithts.</p>
Full article ">Figure 5
<p>This schematic shows the specific implementation structure of the attention mechanism based on probability density. Each module in the diagram represents the various computational steps from input to output, including convolution layers, global average pooling, and final output processing.</p>
Full article ">
19 pages, 4786 KiB  
Article
RT-DETR-Tea: A Multi-Species Tea Bud Detection Model for Unstructured Environments
by Yiyong Chen, Yang Guo, Jianlong Li, Bo Zhou, Jiaming Chen, Man Zhang, Yingying Cui and Jinchi Tang
Agriculture 2024, 14(12), 2256; https://doi.org/10.3390/agriculture14122256 - 10 Dec 2024
Viewed by 55
Abstract
Accurate bud detection is a prerequisite for automatic tea picking and yield statistics; however, current research suffers from missed detection due to the variety of singleness and false detection under complex backgrounds. Traditional target detection models are mainly based on CNN, but CNN [...] Read more.
Accurate bud detection is a prerequisite for automatic tea picking and yield statistics; however, current research suffers from missed detection due to the variety of singleness and false detection under complex backgrounds. Traditional target detection models are mainly based on CNN, but CNN can only achieve the extraction of local feature information, which is a lack of advantages for the accurate identification of targets in complex environments, and Transformer can be a good solution to the problem. Therefore, based on a multi-variety tea bud dataset, this study proposes RT-DETR-Tea, an improved object detection model under the real-time detection Transformer (RT-DETR) framework. This model uses cascaded group attention to replace the multi-head self-attention (MHSA) mechanism in the attention-based intra-scale feature interaction (AIFI) module, effectively optimizing deep features and enriching the semantic information of features. The original cross-scale feature-fusion module (CCFM) mechanism is improved to establish the gather-and-distribute-Tea (GD-Tea) mechanism for multi-level feature fusion, which can effectively fuse low-level and high-level semantic information and large and small tea bud features in natural environments. The submodule of DilatedReparamBlock in UniRepLKNet was employed to improve RepC3 to achieve an efficient fusion of tea bud feature information and ensure the accuracy of the detection head. Ablation experiments show that the precision and mean average precision of the proposed RT-DETR-Tea model are 96.1% and 79.7%, respectively, which are increased by 5.2% and 2.4% compared to those of the original model, indicating the model’s effectiveness. The model also shows good detection performance on the newly constructed tea bud dataset. Compared with other detection algorithms, the improved RT-DETR-Tea model demonstrates superior tea bud detection performance, providing effective technical support for smart tea garden management and production. Full article
Show Figures

Figure 1

Figure 1
<p>Tea tree bud images. (<b>a</b>) Schematic representation of multi-species tea buds. (<b>b</b>) Schematic diagrams of tea buds in different natural environments.</p>
Full article ">Figure 2
<p>Structure diagram of RT-DETR.</p>
Full article ">Figure 3
<p>RT-DETR-Tea network structure.</p>
Full article ">Figure 4
<p>Cascaded Group Attention module architecture.</p>
Full article ">Figure 5
<p>Diagram of the low-GD module structure.</p>
Full article ">Figure 6
<p>High-GD module structure diagram.</p>
Full article ">Figure 7
<p>Structure diagram of the Inject module.</p>
Full article ">Figure 8
<p>DilatedReparamBlock module structure diagram.</p>
Full article ">Figure 9
<p>The result of Grad-CAM and detection results.</p>
Full article ">Figure 9 Cont.
<p>The result of Grad-CAM and detection results.</p>
Full article ">Figure 10
<p>Detection effects of different models.</p>
Full article ">Figure 11
<p>The results of RT-DETR-Tea detection.</p>
Full article ">Figure 12
<p>Comparison of the detection results for different tea bud sizes.</p>
Full article ">Figure 13
<p>Comparison of the detection results for different light intensities.</p>
Full article ">
11 pages, 4199 KiB  
Article
Experimental Study on the Propulsion Performance of Laser Ablation Induced Pulsed Plasma
by Hang Song, Jifei Ye, Ming Wen, Haichao Cui and Wentao Zhao
Aerospace 2024, 11(12), 1013; https://doi.org/10.3390/aerospace11121013 - 9 Dec 2024
Viewed by 316
Abstract
This study investigates the influence of electromagnetic fields on the propulsion performance of laser plasma propulsion. Based on the principle of pulsed plasma thrusters, an electromagnetic field is utilized to accelerate laser plasma, achieving enhanced propulsion performance. This approach represents a novel method [...] Read more.
This study investigates the influence of electromagnetic fields on the propulsion performance of laser plasma propulsion. Based on the principle of pulsed plasma thrusters, an electromagnetic field is utilized to accelerate laser plasma, achieving enhanced propulsion performance. This approach represents a novel method for the electromagnetic enhancement of laser propulsion performance. In this paper, pulsed plasma thrusters induced by laser ablation are employed. The generated plasma is subjected to the Lorentz force under the influence of an electromagnetic field to obtain higher speed, thus increasing impulse and specific impulse. An experimental platform for laser-ablation plasma electromagnetic acceleration was constructed to explore the enhancement effect of discharge characteristics and propulsion performance. The results demonstrate that increased laser energy has little effect on discharge characteristics, while the trend of propulsion performance parameters initially rises and then declines. After coupling the electromagnetic field, the propulsion performance is significantly enhanced, with stronger electromagnetic fields yielding more pronounced effects. Full article
Show Figures

Figure 1

Figure 1
<p>Schematic diagram.</p>
Full article ">Figure 2
<p>Schematic diagram of the experimental apparatus.</p>
Full article ">Figure 3
<p>Laser (<b>left</b>) and high-voltage power supply (<b>right</b>).</p>
Full article ">Figure 4
<p>Physical diagram of the micro impulse measuring device based on the torsion pendulum test bench.</p>
Full article ">Figure 5
<p>Torsion pendulum displacement change curve.</p>
Full article ">Figure 6
<p>Discharge current change curve: (<b>a</b>) the laser energy E = 730 mJ; (<b>b</b>) the laser energy E = 358 mJ.</p>
Full article ">Figure 7
<p>(<b>a</b>) Impulse change curve with charging voltage; (<b>b</b>) impulse change curve with laser energy.</p>
Full article ">Figure 8
<p>(<b>a</b>) Impulse coefficient change curve with charging voltage; (<b>b</b>) impulse coefficient change curve with laser energy.</p>
Full article ">Figure 9
<p>(<b>a</b>) Specific impulse change curve with charging voltage; (<b>b</b>) specific impulse change curve with laser energy.</p>
Full article ">Figure 10
<p>(<b>a</b>) Efficiency change curve with charging voltage; (<b>b</b>) efficiency change curve with laser energy.</p>
Full article ">
25 pages, 2726 KiB  
Article
HybridGNN: A Self-Supervised Graph Neural Network for Efficient Maximum Matching in Bipartite Graphs
by Chun-Hu Pan, Yi Qu, Yao Yao and Mu-Jiang-Shan Wang
Symmetry 2024, 16(12), 1631; https://doi.org/10.3390/sym16121631 - 9 Dec 2024
Viewed by 310
Abstract
Solving maximum matching problems in bipartite graphs is critical in fields such as computational biology and social network analysis. This study introduces HybridGNN, a novel Graph Neural Network model designed to efficiently address complex matching problems at scale. HybridGNN leverages a combination of [...] Read more.
Solving maximum matching problems in bipartite graphs is critical in fields such as computational biology and social network analysis. This study introduces HybridGNN, a novel Graph Neural Network model designed to efficiently address complex matching problems at scale. HybridGNN leverages a combination of Graph Attention Networks (GATv2), Graph SAGE (SAGEConv), and Graph Isomorphism Networks (GIN) layers to enhance computational efficiency and model performance. Through extensive ablation experiments, we identify that while the SAGEConv layer demonstrates suboptimal performance in terms of accuracy and F1-score, configurations incorporating GATv2 and GIN layers show significant improvements. Specifically, in six-layer GNN architectures, the combinations of GATv2 and GIN layers with ratios of 4:2 and 5:1 yield superior accuracy and F1-score. Therefore, we name these GNN configurations HybridGNN1 and HybridGNN2. Additionally, techniques such as mixed precision training, gradient accumulation, and Jumping Knowledge networks are integrated to further optimize performance. Evaluations on an email communication dataset reveal that HybridGNNs outperform traditional algorithms such as the Hopcroft–Karp algorithm, the Hungarian algorithm, and the Blossom/Edmonds’ algorithm, particularly for large and complex graphs. These findings highlight HybridGNN’s robust capability to solve maximum matching problems in bipartite graphs, making it a powerful tool for analyzing large-scale and intricate graph data. Furthermore, our study aligns with the goals of the Symmetry and Asymmetry Study in Graph Theory special issue by exploring the role of symmetry in bipartite graph structures. By leveraging GNNs, we address the challenges related to symmetry and asymmetry in graph properties, thereby improving the reliability and fault tolerance of complex networks. Full article
(This article belongs to the Special Issue Symmetry and Asymmetry Study in Graph Theory)
Show Figures

Figure 1

Figure 1
<p>Comparison of classical algorithm and GNN model for bipartite matching.</p>
Full article ">Figure 2
<p>Visualization of GNN layer operations. The figure shows how nodes aggregate 1-hop and 2-hop neighborhood information across different GNN layers. The highlighted nodes and edges represent the flow of information, illustrating the step-by-step aggregation process in a GNN model.</p>
Full article ">Figure 3
<p>HybridGNN architectural overview.</p>
Full article ">Figure 4
<p>Comparison of training accuracy over 60 epochs for different GNN configurations.</p>
Full article ">Figure 5
<p>Comparison of training F<sub>1</sub>-score over 60 epochs for different GNN configurations.</p>
Full article ">Figure 6
<p>Comparison of training accuracy over 60 epochs for the experiments replacing SAGE with GATv2 and GIN.</p>
Full article ">Figure 7
<p>Comparison of F<sub>1</sub>-score over 60 epochs for the experiments replacing SAGE with GATv2 and GIN.</p>
Full article ">Figure 8
<p>Training accuracy comparison over 60 epochs for different GNN configurations.</p>
Full article ">Figure 9
<p>Training F<sub>1</sub>-score comparison over 60 epochs for different GNN configurations.</p>
Full article ">Figure 10
<p>Training accuracy comparison over 60 epochs for HybridGNN<sub>1</sub>, HybridGNN<sub>2</sub>, and classical algorithms (Hopcroft–Karp, Hungarian, and Blossom).</p>
Full article ">Figure 11
<p>Training F<sub>1</sub>-score comparison over 60 epochs for HybridGNN<sub>1</sub>, HybridGNN<sub>2</sub>, and classical algorithms (Hopcroft–Karp, Hungarian, and Blossom).</p>
Full article ">Figure 12
<p>Test set accuracy and F<sub>1</sub>-score comparison for HybridGNN<sub>1</sub>, HybridGNN<sub>2</sub>, and classical algorithms (Hopcroft–Karp, Hungarian, Blossom).</p>
Full article ">Figure 13
<p>Comparison of memory usage across HybridGNN models and classical algorithms.</p>
Full article ">Figure A1
Full article ">Figure A2
Full article ">Figure A3
Full article ">Figure A4
Full article ">
18 pages, 1071 KiB  
Article
PMHR: Path-Based Multi-Hop Reasoning Incorporating Rule-Enhanced Reinforcement Learning and KG Embeddings
by Ang Ma, Yanhua Yu, Chuan Shi, Shuai Zhen, Liang Pang and Tat-Seng Chua
Electronics 2024, 13(23), 4847; https://doi.org/10.3390/electronics13234847 - 9 Dec 2024
Viewed by 271
Abstract
Multi-hop reasoning provides a means for inferring indirect relationships and missing information from knowledge graphs (KGs). Reinforcement learning (RL) was recently employed for multi-hop reasoning. Although RL-based methods provide explainability, they face challenges such as sparse rewards, spurious paths, large action spaces, and [...] Read more.
Multi-hop reasoning provides a means for inferring indirect relationships and missing information from knowledge graphs (KGs). Reinforcement learning (RL) was recently employed for multi-hop reasoning. Although RL-based methods provide explainability, they face challenges such as sparse rewards, spurious paths, large action spaces, and long training and running times. In this study, we present a novel approach that combines KG embeddings and RL strategies for multi-hop reasoning called path-based multi-hop reasoning (PMHR). We address the issues of sparse rewards and spurious paths by incorporating a well-designed reward function that combines soft rewards with rule-based rewards. The rewards are adjusted based on the target entity and the path to it. Furthermore, we perform action filtering and utilize the vectors of entities and relations acquired through KG embeddings to initialize the environment, thereby significantly reducing the runtime. Experiments involving a comprehensive performance evaluation, efficiency analysis, ablation studies, and a case study were performed. The experimental results on benchmark datasets demonstrate the effectiveness of PMHR in improving KG reasoning accuracy while preserving interpretability. Compared to existing state-of-the-art models, PMHR achieved Hit@1 improvements of 0.63%, 2.02%, and 3.17% on the UMLS, Kinship, and NELL-995 datasets, respectively. PMHR provides not only improved reasoning accuracy and explainability but also optimized computational efficiency, thereby offering a robust solution for multi-hop reasoning. Full article
(This article belongs to the Special Issue Future Technologies for Data Management, Processing and Application)
Show Figures

Figure 1

Figure 1
<p>An example of a KG. Solid edges are observed and the dashed edge is a query.</p>
Full article ">Figure 2
<p>An overview of PMHR model.</p>
Full article ">Figure 3
<p>The distribution of the shortest path lengths.</p>
Full article ">Figure 4
<p>The efficiency comparison between PMHR and path-based models.</p>
Full article ">Figure 5
<p>Ablation study on key parts of PMHR.</p>
Full article ">
27 pages, 1826 KiB  
Article
Backdoor Attack Against Dataset Distillation in Natural Language Processing
by Yuhao Chen, Weida Xu, Sicong Zhang and Yang Xu
Appl. Sci. 2024, 14(23), 11425; https://doi.org/10.3390/app142311425 - 9 Dec 2024
Viewed by 412
Abstract
Dataset distillation has become an important technique for enhancing the efficiency of data when training machine learning models. It finds extensive applications across various fields, including computer vision (CV) and natural language processing (NLP). However, it essentially consists of a deep neural network [...] Read more.
Dataset distillation has become an important technique for enhancing the efficiency of data when training machine learning models. It finds extensive applications across various fields, including computer vision (CV) and natural language processing (NLP). However, it essentially consists of a deep neural network (DNN) model, which remain susceptible to security and privacy vulnerabilities (e.g., backdoor attacks). Existing studies have primarily focused on optimizing the balance between computational efficiency and model performance, overlooking the accompanying security and privacy risks. This study presents the first backdoor attack targeting NLP models trained on distilled datasets. We introduce malicious triggers into synthetic data during the distillation phase to execute a backdoor attack on downstream models trained with these data. We employ several widely used datasets to assess how different architectures and dataset distillation techniques withstand our attack. The experimental findings reveal that the attack achieves strong performance with a high (above 0.9 and up to 1.0) attack success rate (ASR) in most cases. For backdoor attacks, high attack performance often comes at the cost of reduced model utility. Our attack maintains high ASR while maximizing the preservation of downstream model utility, as evidenced by results showing that the clean test accuracy (CTA) of the backdoored model is very close to that of the clean model. Additionally, we performed comprehensive ablation studies to identify key factors affecting attack performance. We tested our attack method against five defense strategies, including NAD, Neural Cleanse, ONION, SCPD, and RAP. The experimental results show that these defense methods are unable to reduce the attack success rate without compromising the model’s performance on normal tasks. Therefore, these methods cannot effectively defend against our attack. Full article
(This article belongs to the Section Computing and Artificial Intelligence)
Show Figures

Figure 1

Figure 1
<p>Overview of dataset distillation.</p>
Full article ">Figure 2
<p>Process of backdoor attack in NLP.</p>
Full article ">Figure 3
<p>Overview of BAMDD-NLP against DwAL.</p>
Full article ">Figure 4
<p>Overview of BAMDD-NLP against DiLM.</p>
Full article ">Figure 5
<p>(<b>a</b>) The <span class="html-italic">ASR</span> scores of BAMDD-NLP across various poisoning ratios and model architectures for DwAL and (<b>b</b>) the <span class="html-italic">CTA</span> scores of BAMDD-NLP across various poisoning ratios and model architectures for DwAL.</p>
Full article ">Figure 6
<p>(<b>a</b>) The <span class="html-italic">ASR</span> scores of BAMDD-NLP across various poisoning ratios and model architectures for DiLM and (<b>b</b>) the <span class="html-italic">CTA</span> scores of BAMDD-NLP across various poisoning ratios and model architectures for DiLM.</p>
Full article ">Figure 7
<p>(<b>a</b>) The <span class="html-italic">ASR</span> scores of BAMDD-NLP with DPC <math display="inline"><semantics> <mrow> <mo>∈</mo> <mo>{</mo> <mn>1</mn> <mo>,</mo> <mn>5</mn> <mo>,</mo> <mn>10</mn> <mo>,</mo> <mn>20</mn> <mo>,</mo> <mn>50</mn> <mo>,</mo> <mn>100</mn> <mo>,</mo> <mn>200</mn> <mo>}</mo> </mrow> </semantics></math> for DiLM and (<b>b</b>) the <span class="html-italic">CTA</span> scores of BAMDD-NLP with DPC <math display="inline"><semantics> <mrow> <mo>∈</mo> <mo>{</mo> <mn>1</mn> <mo>,</mo> <mn>5</mn> <mo>,</mo> <mn>10</mn> <mo>,</mo> <mn>20</mn> <mo>,</mo> <mn>50</mn> <mo>,</mo> <mn>100</mn> <mo>,</mo> <mn>200</mn> <mo>}</mo> </mrow> </semantics></math> for DiLM.</p>
Full article ">
14 pages, 2453 KiB  
Article
A Stable Multi-Object Tracking Method for Unstable and Irregular Maritime Environments
by Young-Suk Han and Jae-Yoon Jung
J. Mar. Sci. Eng. 2024, 12(12), 2252; https://doi.org/10.3390/jmse12122252 - 7 Dec 2024
Viewed by 291
Abstract
In this study, an improved stable multi-object simple online and real-time tracking (StableSORT) algorithm that was specifically designed for maritime environments was proposed to address challenges such as camera instability and irregular object motion. Specifically, StableSORT integrates a buffered IoU (B-IoU) and an [...] Read more.
In this study, an improved stable multi-object simple online and real-time tracking (StableSORT) algorithm that was specifically designed for maritime environments was proposed to address challenges such as camera instability and irregular object motion. Specifically, StableSORT integrates a buffered IoU (B-IoU) and an observation-adaptive Kalman filter (OAKF) into the StrongSORT framework to improve tracking accuracy and robustness. A dataset was collected along the southern coast of Korea using a small autonomous surface vehicle to capture real-world maritime conditions. On this dataset, StableSORT achieved a 2.7% improvement in HOTA, 4.9% in AssA, and 2.6% in IDF1 compared to StrongSORT, and it significantly outperformed ByteTrack and OC-SORT by 84% and 69% in HOTA, respectively. These results underscore StableSORT’s ability to maintain identity consistency and enhance tracking performance under challenging maritime conditions. The ablation studies further validated the contributions of the B-IoU and OAKF modules in maintaining identity consistency and tracking accuracy under challenging maritime conditions. Full article
(This article belongs to the Special Issue Unmanned Marine Vehicles: Navigation, Control and Sensing)
Show Figures

Figure 1

Figure 1
<p>Flow diagram of StableSORT based on StrongSORT with B-IoU and OAKF enhancements.</p>
Full article ">Figure 2
<p>Examples of objects with unstable and irregular motion from the test sequences. The green line shows the trace of the object in the image.</p>
Full article ">Figure 3
<p>YOLOv5x model training and validation results over epochs.</p>
Full article ">Figure 4
<p>Examples of tracking results for StrongSORT and StableSORT.</p>
Full article ">
12 pages, 3245 KiB  
Article
GDE-Pose: A Real-Time Adaptive Compression and Multi-Scale Dynamic Feature Fusion Approach for Pose Estimation
by Kaiian Kuok, Xuan Liu, Jinwei Ye, Yaokang Wang and Wenjian Liu
Electronics 2024, 13(23), 4837; https://doi.org/10.3390/electronics13234837 - 7 Dec 2024
Viewed by 305
Abstract
This paper introduces a novel lightweight pose estimation model, GDE-pose, which addresses the current trade-off between accuracy and computational efficiency in existing models. GDE-pose builds upon the baseline YOLO-pose model by incorporating Ghost Bottleneck, a Dynamic Feature Fusion Module (DFFM), and ECA Attention [...] Read more.
This paper introduces a novel lightweight pose estimation model, GDE-pose, which addresses the current trade-off between accuracy and computational efficiency in existing models. GDE-pose builds upon the baseline YOLO-pose model by incorporating Ghost Bottleneck, a Dynamic Feature Fusion Module (DFFM), and ECA Attention to achieve more effective feature representation and selection. The Ghost Bottleneck reduces computational complexity, DFFM enhances multi-scale feature fusion, and ECA Attention optimizes the selection of key features. GDE-pose improves pose estimation accuracy while preserving real-time performance. Experimental results demonstrate that GDE-pose achieves higher accuracy on the COCO dataset, with a substantial reduction in parameters, over 80% fewer FLOPs, and an increased inference speed of 31 FPS, underscoring its exceptional lightweight and real-time capabilities. Ablation studies confirm the independent contribution of each module to the model’s overall performance. GDE-pose’s design highlights its broad applicability in real-time pose estimation tasks. Full article
Show Figures

Figure 1

Figure 1
<p>Overall architecture of GDE-pose.</p>
Full article ">Figure 2
<p>(<b>a</b>) C3k2_Ghost (<b>b</b>) C3k2_DFFM.</p>
Full article ">Figure 3
<p>Diagram of C3k2_DFFM.</p>
Full article ">Figure 4
<p>Illustration of GDE-pose performance.</p>
Full article ">Figure 5
<p>Illustration of loss results.</p>
Full article ">
26 pages, 11259 KiB  
Article
Axial-UNet++ Power Line Detection Network Based on Gated Axial Attention Mechanism
by Ding Hu, Zihao Zheng, Yafei Liu, Chengkang Liu and Xiaoguo Zhang
Remote Sens. 2024, 16(23), 4585; https://doi.org/10.3390/rs16234585 - 6 Dec 2024
Viewed by 326
Abstract
The segmentation and recognition of power lines are crucial for the UAV-based inspection of overhead power lines. To address the issues of class imbalance, low sample quantity, and long-range dependency in images, a specialized semantic segmentation network for power line segmentation called Axial-UNet++ [...] Read more.
The segmentation and recognition of power lines are crucial for the UAV-based inspection of overhead power lines. To address the issues of class imbalance, low sample quantity, and long-range dependency in images, a specialized semantic segmentation network for power line segmentation called Axial-UNet++ is proposed. Firstly, to tackle the issue of long-range dependencies in images and low sample quantity, a gated axial attention mechanism is introduced to expand the receptive field and improve the capture of relative positional biases in small datasets, thereby proposing a novel feature extraction module termed axial-channel local normalization module. Secondly, to address the imbalance in training samples, a new loss function is developed by combining traditional binary cross-entropy loss with focal loss, enhancing the precision of image semantic segmentation. Lastly, ablation and comparative experiments on the PLDU and Mendeley datasets demonstrate that the proposed model achieves 54.7% IoU and 80.1% recall on the PLDU dataset, and 79.3% IoU and 93.1% recall on the Mendeley dataset, outperforming other listed models. Additionally, robustness experiments show the adaptability of the Axial-UNet++ model under extreme conditions and the augmented image dataset used in this study has been open sourced. Full article
Show Figures

Figure 1

Figure 1
<p>The overall structure of UNet++. The superscript for <math display="inline"><semantics> <msup> <mi>X</mi> <mrow> <mi>i</mi> <mo>,</mo> <mi>j</mi> </mrow> </msup> </semantics></math> denotes the <span class="html-italic">j</span>-th module in the <span class="html-italic">i</span>-th layer.</p>
Full article ">Figure 2
<p>Convolutional block of UNet++.</p>
Full article ">Figure 3
<p>The overall structure of axial-channel local normalization module.</p>
Full article ">Figure 4
<p>Gated axial attention mechanism. The matrices <math display="inline"><semantics> <msub> <mi>W</mi> <mi>Q</mi> </msub> </semantics></math>, <math display="inline"><semantics> <msub> <mi>W</mi> <mi>K</mi> </msub> </semantics></math>, and <math display="inline"><semantics> <msub> <mi>W</mi> <mi>V</mi> </msub> </semantics></math> correspond to the parameter matrices for the query term <math display="inline"><semantics> <msub> <mi>q</mi> <mn>0</mn> </msub> </semantics></math>, the key term <math display="inline"><semantics> <msub> <mi>k</mi> <mn>0</mn> </msub> </semantics></math>, and the value term <math display="inline"><semantics> <msub> <mi>v</mi> <mn>0</mn> </msub> </semantics></math>, respectively.</p>
Full article ">Figure 5
<p>The overall structure of Axial-UNet++. The superscript for <math display="inline"><semantics> <msup> <mi>X</mi> <mrow> <mi>i</mi> <mo>,</mo> <mi>j</mi> </mrow> </msup> </semantics></math> and <math display="inline"><semantics> <msup> <mi>A</mi> <mrow> <mi>i</mi> <mo>,</mo> <mi>j</mi> </mrow> </msup> </semantics></math> denotes the <span class="html-italic">j</span>-th module in the <span class="html-italic">i</span>-th layer.</p>
Full article ">Figure 6
<p>Example images of power lines and their corresponding ground truth annotations from the PLDU and Mendeley datasets.</p>
Full article ">Figure 7
<p>Comparison of experimental results with different attention feature extraction modules.</p>
Full article ">Figure 8
<p>Comparison of experimental results with general semantic segmentation models.</p>
Full article ">Figure 9
<p>Comparison of experimental results with power line segmentation specialized models.</p>
Full article ">Figure 10
<p>Original images.</p>
Full article ">Figure 11
<p>Experimental results in a foggy environment. In the predicted results, FN pixels are represented in red, while FP pixels are indicated in green.</p>
Full article ">Figure 12
<p>Experimental results in a snowy environment. In the predicted results, FN pixels are represented in red, while FP pixels are indicated in green.</p>
Full article ">Figure 13
<p>Experimental results in a strong light environment. In the predicted results, FN pixels are represented in red, while FP pixels are indicated in green.</p>
Full article ">Figure 14
<p>Experimental results in a motion blur environment. In the predicted results, FN pixels are represented in red, while FP pixels are indicated in green.</p>
Full article ">
13 pages, 2174 KiB  
Article
Leveraging Femtosecond Laser Ablation for Tunable Near-Infrared Optical Properties in MoS2-Gold Nanocomposites
by Ilya A. Zavidovskiy, Ilya V. Martynov, Daniil I. Tselikov, Alexander V. Syuy, Anton A. Popov, Sergey M. Novikov, Andrei V. Kabashin, Aleksey V. Arsenin, Gleb I. Tselikov, Valentyn S. Volkov and Alexey D. Bolshakov
Nanomaterials 2024, 14(23), 1961; https://doi.org/10.3390/nano14231961 - 6 Dec 2024
Viewed by 415
Abstract
Transition metal dichalcogenides (TMDCs), particularly molybdenum disulfide (MoS2), have gained significant attention in the field of optoelectronics and photonics due to their unique electronic and optical properties. The integration of TMDCs with plasmonic materials allows to tailor the optical response and [...] Read more.
Transition metal dichalcogenides (TMDCs), particularly molybdenum disulfide (MoS2), have gained significant attention in the field of optoelectronics and photonics due to their unique electronic and optical properties. The integration of TMDCs with plasmonic materials allows to tailor the optical response and offers significant advantages for photonic applications. This study presents a novel approach to synthesize MoS2-Au nanocomposites utilizing femtosecond laser ablation in liquid to achieve tunable optical properties in the near-infrared (NIR) region. By adjusting ablation and fragmentation protocols, we successfully synthesize various core–shell and core–shell–satellite nanoparticle composites, such as MoS2/MoSxOy, MoSxOy/Au, and MoS2/MoSxOy/Au. UV-visible absorption spectroscopy unveils considerable changes in the optical response of the particles depending on the fabrication regime due to structural modifications. Hybrid nanoparticles exhibit enhanced photothermal properties when subjected to NIR-I laser irradiation, demonstrating potential benefits for selective photothermal therapy. Our findings underscore that the engineered nanocomposites not only facilitate green synthesis but also pave the way for tailored therapeutic applications, highlighting their role as promising candidates in the field of nanophotonics and cancer treatment. Full article
(This article belongs to the Special Issue Optical Composites, Nanophotonics and Metamaterials)
Show Figures

Figure 1

Figure 1
<p>Schematic representation of the one-, two-, and three-step processes as well as solution mixing for the synthesis of pristine and hybrid MoS<sub>2</sub>/Au NPs.</p>
Full article ">Figure 2
<p>TEM characterization of one-step synthesized NPs. (<b>a</b>,<b>d</b>) TEM images of the ablated MoS<sub>2</sub> (<b>a</b>) and Au (<b>d</b>) NPs. Scale bar, 50 nm. (<b>b</b>,<b>e</b>) Size distributions of MoS<sub>2</sub> (<b>b</b>) and Au (<b>e</b>) NPs. (<b>c</b>,<b>f</b>) SAED patterns of MoS<sub>2</sub> (<b>c</b>) and Au (<b>f</b>) NPs.</p>
Full article ">Figure 3
<p>Characterization of two- and three-step synthesized NPs and Raman spectroscopy data. TEM images of (<b>a</b>) “Au in MoS<sub>2</sub>”, (<b>b</b>)“MoS<sub>2</sub> in Au”, and (<b>c</b>) “MoS<sub>2</sub>:Au co-fragmented” NPs. Scale bar, 50 nm. Size distributions of (<b>d</b>) “Au in MoS<sub>2</sub>”, (<b>e</b>) “MoS<sub>2</sub> in Au”, and (<b>f</b>) “MoS<sub>2</sub>:Au co-fragmented” NPs. Turquoise bars represent Au NPs, while orange bars represent MoS<sub>2</sub>-based NPs. (<b>g</b>) Raman spectra of the NPs. Violet, brown- and green-colored numbers indicate the positions of the peaks related to MoS<sub>2</sub>, MoS<sub>x</sub>O<sub>y</sub>, and MoO<sub>x</sub>, respectively. Inset shows the magnified low-intensity peaks of the MoS<sub>2</sub> sample.</p>
Full article ">Figure 4
<p>High-angle annular dark-field imaging (upper left panels) and EDX maps of “MoS<sub>2</sub>” (<b>a</b>), “MoS<sub>2</sub> in Au” (<b>b</b>), and “Au in MoS<sub>2</sub>” (<b>c</b>) NPs. Scale bar, 20 nm.</p>
Full article ">Figure 5
<p>Optical absorption and photoheating. (<b>a</b>) UV-visible extinction spectra. Red dotted line indicates the photoheating laser wavelength. (<b>b</b>–<b>g</b>) Photoheating dynamics. ΔT<sub>max</sub> and PCE notations indicate the values of maximum temperature increases observed throughout the heating and photothermal conversion efficiencies. The plots are presented in the following order: (<b>b</b>) MoS2, (<b>c</b>) Au, (<b>d</b>) MoS<sub>2</sub> in Au, (<b>e</b>) Au in MoS<sub>2</sub>, (<b>f</b>) MoS<sub>2</sub>:Au co-fragmented, (<b>g</b>) MoS<sub>2</sub>+Au.</p>
Full article ">
30 pages, 11972 KiB  
Article
Identifying Infant Body Position from Inertial Sensors with Machine Learning: Which Parameters Matter?
by Joanna Duda-Goławska, Aleksander Rogowski, Zuzanna Laudańska, Jarosław Żygierewicz and Przemysław Tomalski
Sensors 2024, 24(23), 7809; https://doi.org/10.3390/s24237809 - 6 Dec 2024
Viewed by 317
Abstract
The efficient classification of body position is crucial for monitoring infants’ motor development. It may fast-track the early detection of developmental issues related not only to the acquisition of motor milestones but also to postural stability and movement patterns. In turn, this may [...] Read more.
The efficient classification of body position is crucial for monitoring infants’ motor development. It may fast-track the early detection of developmental issues related not only to the acquisition of motor milestones but also to postural stability and movement patterns. In turn, this may facilitate and enhance opportunities for early intervention that are crucial for promoting healthy growth and development. The manual classification of human body position based on video recordings is labour-intensive, leading to the adoption of Inertial Motion Unit (IMU) sensors. IMUs measure acceleration, angular velocity, and magnetic field intensity, enabling the automated classification of body position. Many research teams are currently employing supervised machine learning classifiers that utilise hand-crafted features for data segment classification. In this study, we used a longitudinal dataset of IMU recordings made in the lab in three different play activities of infants aged 4–12 months. The classification was conducted based on manually annotated video recordings. We found superior performance of the CatBoost Classifier over the Random Forest Classifier in the task of classifying five positions based on IMU sensor data from infants, yielding excellent classification accuracy of the Supine (97.7%), Sitting (93.5%), and Prone (89.9%) positions. Moreover, using data ablation experiments and analysing the SHAP (SHapley Additive exPlanations) values, the study assessed the importance of various groups of features from both the time and frequency domains. The results highlight that both accelerometer and magnetometer data, especially their statistical characteristics, are critical contributors to improving the accuracy of body position classification. Full article
Show Figures

Figure 1

Figure 1
<p>Placement of all sensors on the infant and caregiver. The Developmental Neurocognition Laboratory Babylab provided the photos at the Institute of Psychology, Polish Academy of Sciences, with written consent from the caregiver for publication.</p>
Full article ">Figure 2
<p>Percentage of total visit duration spent in various positions for each infant at the 4-, 6-, 9-, and 12-month time points. Each coloured dot represents the contribution of an individual infant. The numbers in brackets at the bottom of each plot indicate the number of infants that did not show a given position at a given time point. These plots illustrate changes in the distribution of postural behaviours as infants develop their gross motor skills over time.</p>
Full article ">Figure 3
<p>Illustration of sensor readings from tri-axial accelerometer, gyroscope, and magnetometer positioned on the left leg of a 12-month-old infant. The data are displayed across three axes (X, Y, Z) for each sensor type, showing variations in movement patterns associated with different body positions.</p>
Full article ">Figure 4
<p>Sliding windows of 2 s with a 1-s overlap, showing assigned position fragments. Blue windows represent the Supine class, and teal windows indicate the Prone class. Windows where less than 75% of samples are consistently assigned the same position label were not assigned to any class.</p>
Full article ">Figure 5
<p>Composition of feature groups. Five distinct feature groups were extracted from each type of signal, including XYZ signals.</p>
Full article ">Figure 6
<p>Comparison of F1 scores for Random Forest and CatBoost models across three sensors and five postures. Parallel coordinate plots highlight the performance differences, with green indicating superior performance by CatBoost and red indicating better performance by Random Forest. Each line corresponds to one fold in the cross-validation. Boxplots provide a visual summary of F1 score distributions for each model and posture. Statistical significance is indicated using FDR-adjusted <span class="html-italic">p</span>-values (the asterisks indicate the significance level * <span class="html-italic">p</span> &lt; 0.05) derived from the Friedman test. The colour bar reflects the mean F1 scores for each posture, offering an overview of model performance across various conditions.</p>
Full article ">Figure 7
<p>A confusion matrix was obtained based on the predictions of CatBoost classifiers trained on the combined set of parameters for two pairs of sensors: Trunk and Legs. The percentage of actual classifications is displayed at the top, with average counts across the five folds and their standard error included in parentheses. The colour scale corresponds to the percentage of the actual position, providing a visual representation of classification accuracy.</p>
Full article ">Figure 8
<p>F1 score change for Catboost models using only one feature group at a time relative to models using all feature groups for two pairs of sensors: Trunk and Legs.</p>
Full article ">Figure 9
<p>F1 score change for models excluding one feature group at a time relative to the CatBoost model using all feature groups for two pairs of sensors: Trunk and Legs.</p>
Full article ">Figure 10
<p>The sum of |SHAP| values across five folds illustrates the features with the highest total impact on the model, categorised by different signals and features. This figure highlights the most influential features in the model’s predictions, with larger-sum |SHAP| values indicating a more significant impact.</p>
Full article ">Figure 11
<p>Mean |SHAP| values across five folds illustrate the features with the highest impact on the model, categorised by different signals and features. Presented values are multiplied by 1 × 10<sup>5</sup> for clarity. This figure highlights the most influential features in the model’s predictions, with elevated SHAP values indicating a more significant impact.</p>
Full article ">Figure 12
<p>Illustration of the correlation between the annotated (actual) and predicted time spent in five distinct positions across study sessions. The x-axis represents the real percentage of time spent in each position, while the y-axis shows the corresponding predicted percentage. Each point in the plot corresponds to a specific infant in a position during one study session, with a line of best fit illustrating the correlation between the real and predicted values. The closer the points align with the line, the more accurate the predictions. Based on the CatBoost model for two pairs of sensors, Trunk and Legs.</p>
Full article ">Figure A1
<p>Visual representation of train–test splitting and 5-fold cross-validation techniques for assessing model performance and optimising hyperparameters. Turquoise indicates the training set, and blue is the test set.</p>
Full article ">Figure A2
<p>F1 values averaged across five folds and corresponding standard error of mean, for pairs of sensors placed on the Trunk; Trunk and Legs; and Trunk, Legs, and Arms for CatBoost and Random Forest.</p>
Full article ">Figure A3
<p>Illustration of the correlation between the annotated (actual) and predicted time spent in five distinct positions across study sessions. The x-axis represents the real percentage of time spent in each position, while the y-axis shows the corresponding predicted percentage. Each point in the plot corresponds to a specific infant in a position during one study session, with a line of best fit illustrating the correlation between the real and predicted values. The closer the points align with the line, the more accurate the predictions. Based on the model for two pairs of sensors: Trunk and Legs.</p>
Full article ">
21 pages, 3566 KiB  
Article
Rapid Vehicle Trajectory Prediction Based on Multi-Attention Mechanism for Fusing Multimodal Information
by Likun Ge, Shuting Wang and Guangqi Wang
Electronics 2024, 13(23), 4806; https://doi.org/10.3390/electronics13234806 - 5 Dec 2024
Viewed by 350
Abstract
Trajectory prediction plays a crucial role in autonomous driving tasks, as accurately and rapidly predicting the future trajectories of traffic participants can significantly enhance the safety and robustness of autonomous driving systems. This paper presents a novel trajectory prediction model that follows the [...] Read more.
Trajectory prediction plays a crucial role in autonomous driving tasks, as accurately and rapidly predicting the future trajectories of traffic participants can significantly enhance the safety and robustness of autonomous driving systems. This paper presents a novel trajectory prediction model that follows the encoder–decoder paradigm, achieving precise and rapid predictions of future vehicle trajectories by efficiently aggregating the spatiotemporal and interaction information of agents in traffic scenarios. We propose an agent–agent interaction information extraction module based on a sparse graph attention mechanism, which enables the efficient aggregation of interaction information between agents. Additionally, we introduce a non-autoregressive query generation method that accelerates the model inference speed by generating the decoding queries in parallel. Comparative experiments with the existing advanced algorithms show that our method improves the multimodal trajectory prediction metrics for the Minimum Average Displacement Error (minADE), the Minimum Final Displacement Error (minFDE), and the Miss Rate (MR) by an average of 9.1%, 11.8%, and 14.6%, respectively, while the inference time is only 33.7% of the average time taken by the other algorithms. Finally, we demonstrate the effectiveness of the various modules proposed in this paper through ablation studies. Full article
(This article belongs to the Section Electrical and Autonomous Vehicles)
Show Figures

Figure 1

Figure 1
<p>Algorithm Structure Diagram.</p>
Full article ">Figure 2
<p>Structure Diagram of the local encoder.</p>
Full article ">Figure 3
<p>The impact of different values of <math display="inline"><semantics> <mi>α</mi> </semantics></math> on the formula.</p>
Full article ">Figure 4
<p>Structure of the query generation module.</p>
Full article ">Figure 5
<p>Impact of network architecture parameters on performance and time consumption. (<b>a</b>) Relationship of minADE with the number of layers. (<b>b</b>) Variation of time consumption with the number of layers.</p>
Full article ">Figure 6
<p>Straight driving scenario. (<b>a</b>) Shows the trajectory prediction process for the next 3 s and its zoomed-in view. (<b>b</b>) Presents the prediction results and zoomed-in views for the other straight driving scenarios.</p>
Full article ">Figure 7
<p>Typical intersection scenario. (<b>a</b>) Illustrates the trajectory prediction process for the next 3 s and its zoomed-in view. (<b>b</b>) Displays the prediction results and zoomed-in views for the other intersection driving scenarios.</p>
Full article ">Figure 7 Cont.
<p>Typical intersection scenario. (<b>a</b>) Illustrates the trajectory prediction process for the next 3 s and its zoomed-in view. (<b>b</b>) Displays the prediction results and zoomed-in views for the other intersection driving scenarios.</p>
Full article ">
18 pages, 7414 KiB  
Article
The microRNA-29ab1/Zfp36/AR Axis in the Hypothalamus Regulates Male-Typical Behaviors in Mice
by Jie Ma, Yingying Lin, Wei Xiong, Xiaoxue Liu, Minghui Pan, Jiazeng Sun, Yanan Sun, Yixuan Li, Huiyuan Guo, Guofang Pang, Xiaoyu Wang and Fazheng Ren
Int. J. Mol. Sci. 2024, 25(23), 13089; https://doi.org/10.3390/ijms252313089 - 5 Dec 2024
Viewed by 299
Abstract
Male-typical behaviors such as aggression and mating, which reflect sexual libido in male mice, are regulated by the hypothalamus, a crucial part of the nervous system. Previous studies have demonstrated that microRNAs (miRNAs), especially miR-29, play a vital role in reproduction and [...] Read more.
Male-typical behaviors such as aggression and mating, which reflect sexual libido in male mice, are regulated by the hypothalamus, a crucial part of the nervous system. Previous studies have demonstrated that microRNAs (miRNAs), especially miR-29, play a vital role in reproduction and the neural control of behaviors. However, it remains unclear whether miR-29 affects reproduction through the hypothalamus-mediated regulation of male-typical behaviors. Here, we constructed two mouse knockout models by ablating either the miR-29ab1 or miR-29b2c cluster. Compared to WT, the ablation of miR-29ab1 in male mice significantly reduced the incidence of aggression by 60% and the incidence of mating by 46.15%. Furthermore, the loss of miR-29ab1 in male mice led to the downregulation of androgen receptor (AR) in the ventromedial hypothalamus. Transcriptomic analysis of the hypothalamus of miR-29ab1-deficient mice revealed inflammatory activation and aberrant expression of genes associated with male-typical behaviors, including Ar, Pgr, Htr4, and Htr2c. Using bioinformatics analysis and dual-luciferase reporter assays, we identified zinc finger protein 36 (Zfp36) as a direct downstream target gene of miR-29ab1. We subsequently showed that ZFP36 colocalized with AR in GT1-7 cells. Furthermore, inhibition of Zfp36 or RelB in GT1-7 cells led to an increase in AR expression. Collectively, our results demonstrate that the miR-29ab1/Zfp36/AR axis in the hypothalamus plays a pivotal role in the regulation of aggression and mating in male mice, providing a potential therapeutic target for treating infertility caused by low libido. Full article
(This article belongs to the Special Issue New Horizon for Non-coding RNAs)
Show Figures

Figure 1

Figure 1
<p>The ablation of <span class="html-italic">miR-29ab1</span> leads to infertility, which may be due to impaired aggressive and mating behaviors in male mice. (<b>A</b>) Pregnancy rate (<span class="html-italic">n</span> = 10). (<b>B</b>) Litter size (<span class="html-italic">n</span> = 15). (<b>C</b>) Representative images showing the genital tract of reproductive tissues in male mice. (<b>D</b>,<b>E</b>) H&amp;E staining of the testis (<b>D</b>) and epididymis (<b>E</b>). (<b>F</b>–<b>H</b>) The epithelial thickness of the caput (<b>F</b>), corpus (<b>G</b>), and cauda epididymis (<b>H</b>) (<span class="html-italic">n</span> = 10). (<b>I</b>) Raster plots showing the individual aggression of WT and <span class="html-italic">miR-29ab1</span><sup>−/−</sup> mice towards an intruder of WT male during the aggressive behavior test (15 min). Blue numerals 1–10 denote that the resident mice are male WT mice, while red numerals 11–20 indicate that the resident mice are male <span class="html-italic">miR-29ab1<sup>−/−</sup></span> mice. Each vertical line represents an attack by the resident male mouse on the intruder male mouse (<span class="html-italic">n</span> = 10). (<b>J</b>–<b>M</b>) Quantitative analysis of aggressive animal behavior. Percentage of animals displaying aggressive behavior (<span class="html-italic">n</span> = 10) (<b>J</b>). Frequency of aggressive behavior (<span class="html-italic">n</span> = 10) (<b>K</b>). Amount of time displaying aggressive behavior (<span class="html-italic">n</span> = 10) (<b>L</b>). Latency to aggressive behavior. Each dot represents a mouse (<span class="html-italic">n</span> = 10) (<b>M</b>). (<b>N</b>) Raster plots showing the individual mounting of WT and <span class="html-italic">miR-29ab1</span><sup>−/−</sup> mice towards female WT mice during the mating behavior test (15 h). Blue numerals 1–13 denote the male WT mice, while red numerals 14–26 indicate the male <span class="html-italic">miR-29ab1<sup>−/−</sup></span> mice. Each vertical line represents a mounting attempt by the male mouse on the female mouse (<span class="html-italic">n</span> = 13). (<b>O</b>–<b>R</b>) Quantitative analysis of mating behavior in animals. Percentage of animals displaying mounting (<span class="html-italic">n</span> = 13) (<b>O</b>). Frequency of mating behavior (<span class="html-italic">n</span> = 13) (<b>P</b>). Time of mating (<span class="html-italic">n</span> = 13) (<b>Q</b>). Latency to mating behavior. Each dot represents a mouse (<span class="html-italic">n</span> = 13) (<b>R</b>). Scale bars, 100 μm; * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001, n.s., not significant.</p>
Full article ">Figure 2
<p><span class="html-italic">MiR-29a</span> and <span class="html-italic">miR-29b</span> are enriched in the hypothalamus, and ablation of <span class="html-italic">miR-29ab1</span> impairs expression of AR in the hypothalamus. (<b>A</b>) <span class="html-italic">miR-29</span> expression profiles in adult mice calculated from the mean expression levels across seven tissues in the TissueAtlas database. (<b>B</b>–<b>D</b>) Relative levels of <span class="html-italic">miR-29a</span> (<b>B</b>), <span class="html-italic">miR-29b</span> (<b>C</b>), and <span class="html-italic">miR-29c</span> (<b>D</b>) in the lung, testis, epididymis, and hypothalamus of adult male WT mice (<span class="html-italic">n</span> = 6). (<b>E</b>) FISH analysis showing U6, scramble, <span class="html-italic">miR-29a</span>, <span class="html-italic">miR-29b</span> probes in the hypothalamus (VMH: white dashed circle). (<b>F</b>) Relative expression of <span class="html-italic">miR-29a</span> and <span class="html-italic">miR-29b</span> in the hypothalamus of WT and <span class="html-italic">miR-29ab1</span><sup>−/−</sup> mice (<span class="html-italic">n</span> = 6). (<b>G</b>) Schematic showing how the receptors of sex steroid hormones control male behaviors. (<b>H</b>) Representative western blot showing AR, CYP19, and ERα protein expression levels in the hypothalamus (<span class="html-italic">n</span> = 3). GAPDH served as the loading control. (<b>I</b>–<b>K</b>) Quantification of western blot data. Relative AR (<b>I</b>), CYP19 (<b>J</b>), and ERα (<b>K</b>) expression levels in the hypothalamus were normalized to GAPDH (<span class="html-italic">n</span> = 3). (<b>L</b>) Representative immunohistochemical image showing AR staining in the hypothalamus (VMH: black dashed circle). (<b>M</b>) The number of AR<sup>+</sup> cells in the VMH of WT and <span class="html-italic">miR-29ab1</span><sup>−/−</sup> mice (<span class="html-italic">n</span> = 6). (<b>N</b>) Representative immunohistochemical image showing ERα staining in the hypothalamus (VMH: black dashed circle). (<b>O</b>) The number of ERα<sup>+</sup> cells in the VMH of WT and <span class="html-italic">miR-29ab1</span><sup>−/−</sup> mice (<span class="html-italic">n</span> = 6). Scale bars, 100 μm; MAT, mesenteric adipose tissue, RPM, Reads Per Million; 3V, third ventricle; VMH, ventromedial hypothalamus; AR, androgen receptor; CYP19, Cytochrome P450 19; ERα, estrogen receptor alpha; DAPI, 4′6-diamidino-2-phenylindole. * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001, n.s., not significant.</p>
Full article ">Figure 3
<p>Transcriptomic characteristics of the hypothalamus of WT and <span class="html-italic">miR-29ab1</span><sup>−/−</sup> mice (<span class="html-italic">n</span> = 3). (<b>A</b>) Volcano plot showing upregulated (red), downregulated (blue), and non-significantly changed (grey) DEGs (adjust <span class="html-italic">p</span>-value &lt; 0.05, |log<sub>2</sub>FoldChange| &gt; 2). (<b>B</b>) Number of upregulated, downregulated, and nonsignificant DEGs. (<b>C</b>,<b>D</b>) GO analysis of upregulated (<b>C</b>) and downregulated DEGs (<b>D</b>). (<b>E</b>) KEGG analysis of up-regulated (red) and down-regulated (blue) DEGs. (<b>F</b>) Representative immunofluorescence images showing Iba1<sup>+</sup> microglia in the hypothalamus of WT and <span class="html-italic">miR-29ab1</span><sup>−/−</sup> mice. Scale bars, 50 μm. (<b>G</b>) The number of Iba1<sup>+</sup> cells in the hypothalamus of WT and <span class="html-italic">miR-29ab1</span><sup>−/−</sup> mice (<span class="html-italic">n</span> = 6). (<b>H</b>–<b>J</b>) RT-qPCR analysis of three pro-inflammatory cytokines, TNF-α (<b>H</b>), IL-6 (<b>I</b>), and IL-1β (<b>J</b>) in WT and <span class="html-italic">miR-29ab1</span><sup>−/−</sup> mice (<span class="html-italic">n</span> = 6). (<b>K</b>) Heatmap of genes involved in male-typical behaviors (* adjust <span class="html-italic">p</span>-value &lt; 0.05, ** adjust <span class="html-italic">p</span>-value &lt; 0.01 for heatmap). DEGs, differentially expressed genes; GO, Gene Ontology; KEGG, Kyoto encyclopedia of genes and genomes; KO, <span class="html-italic">miR-29ab1</span><sup>−/−</sup> mice. * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01.</p>
Full article ">Figure 4
<p><span class="html-italic">Zfp36</span> is the target gene of <span class="html-italic">miR-29a/b</span>, and highly expressed in the hypothalamus of <span class="html-italic">miR-29ab1</span><sup>−/−</sup> mice. (<b>A</b>) Venn diagram of overlapping genes among the upregulated DEGs in the hypothalamus, as identified from TargetScanMouse and miRWalk datasets. (<b>B</b>,<b>C</b>) Minimum free energy (mfe) of the <span class="html-italic">miR-29a</span> (<b>B</b>) and <span class="html-italic">miR-29b</span> (<b>C</b>) seed sequence and the 3′-UTR of the predicted target genes from the RNAhybrid dataset. The green strand indicates the predicted miRNA strand (<span class="html-italic">miR-29a</span> or <span class="html-italic">miR-29b</span>), while the red strand indicates the target (<span class="html-italic">Zfp36</span>) in the mouse genome. (<b>D</b>) The top 10 hub genes among the upregulated DEGs in the hypothalamus and calculated by MCC method. Colors indicate the score of the genes. The red color denotes the highest degree, while the yellow color signifies the lowest degree. The linkage represents an interaction between the two proteins. (<b>E</b>) Representative immunofluorescence images showing ZFP36 staining in the hypothalamus of WT and <span class="html-italic">miR-29ab1</span><sup>−/−</sup> mice. The image on the right is a magnified image of the white dashed box on the left. Scale bars, 50 μm. (<b>F</b>) RT-qPCR analysis of <span class="html-italic">Zfp36</span> mRNA levels in WT and <span class="html-italic">miR-29ab1</span><sup>−/−</sup> mice, and each dot represents a sample (<span class="html-italic">n</span> = 6). (<b>G</b>) Representative western blot showing ZFP36 protein expression levels in the hypothalamus of <span class="html-italic">miR-29ab1</span><sup>−/−</sup> mice relative to WT (<span class="html-italic">n</span> = 3). GAPDH served as the loading control. (<b>H</b>) Quantification of western blot data. Relative ZFP36 protein expression levels in the hypothalamus were normalized to GAPDH (<span class="html-italic">n</span> = 6). (<b>I</b>,<b>J</b>) Relative <span class="html-italic">miR-29a</span> (<b>I</b>) and <span class="html-italic">miR-29b</span> (<b>J</b>) expression levels in GT1-7 cells treated with NC mimic/inhibitor, <span class="html-italic">miR-29a/b</span> mimic/inhibitor (<span class="html-italic">n</span> = 6). (<b>K</b>) Representative western blot showing ZFP36 protein expression levels in GT1-7 cells treated with NC mimic/inhibitor, or <span class="html-italic">miR-29a/b</span> mimic/inhibitor (<span class="html-italic">n</span> = 3). GAPDH served as a loading control. The blue number shows the ratio of average intensity of ZFP36/GAPDH. (<b>L</b>) The predicted binding locations for <span class="html-italic">miR-29a-3p</span> and <span class="html-italic">miR-29b-3p</span> on the 3′-UTR of <span class="html-italic">Zfp36</span>. (<b>M</b>) Overexpression of <span class="html-italic">miR-29a/b</span> led to reduced expression of luciferase reporter gene, dependent on the <span class="html-italic">Zfp36</span> 3′-UTR. GT1-7 cells were transferred with luciferase reporter genes regulated by either WT or Mut <span class="html-italic">Zfp36</span> 3′-UTR, along with NC RNA mimic or <span class="html-italic">miR-29a/b</span> mimic duplexes (<span class="html-italic">n</span> = 6/group). 3V, third ventricle; mfe, minimum free energy; MCC, Matthews Correlation Coefficient; ZFP36, zinc finger protein 36; DAPI, 4′6-diamidino-2-phenylindole. * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001.</p>
Full article ">Figure 5
<p><span class="html-italic">Zfp36</span> targets RelB to inhibit AR expression. (<b>A</b>) Heatmap of 11 genes activated in the NF-κB signaling pathway. (<b>B</b>) Correlation analysis of <span class="html-italic">Zfp36</span> and 11 genes involved in the NF-κB signaling pathway. (<b>C</b>) Correlation analysis of <span class="html-italic">Ar</span> and 11 genes involved in the NF-κB signaling pathway. (<b>D</b>) Representative immunofluorescence images showing RelB staining in the hypothalamus of WT and <span class="html-italic">miR-29ab1</span><sup>−/−</sup> mice. Scale bar, 20 μm. (<b>E</b>) The number of RelB<sup>+</sup> cells in the hypothalamus of WT and <span class="html-italic">miR-29ab1</span><sup>−/−</sup> mice. Each dot represents a sample (<span class="html-italic">n</span> = 6). (<b>F</b>) Representative images showing the costaining of ZFP36 and GnRH1 in the hypothalamus. Scale bar, 20 μm. (<b>G</b>) Representative images showing costaining of ZFP36 and AR in GT1-7 cells. White arrows indicate co-localized cells. Scale bar, 20 μm. (<b>H</b>,<b>I</b>) Representative images of western blot showing AR, RelB, and ZFP36 protein expression after treatment of GT1-7 cells with siZfp36 (<b>H</b>) or siRelB (<b>I</b>) for 48 h. siControl samples received scrambled siRNA (siCtrl); 3V, third ventricle; ZFP36, zinc finger protein 36; GnRH1, gonadotropin-releasing hormone 1; AR, androgen receptor; DAPI, 4′6-diamidino-2-phenylindole. * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001.</p>
Full article ">
25 pages, 15227 KiB  
Article
Mechanism of Multi-Physical Fields Coupling in Macro-Area Processing via Laser–Electrochemical Hybrid Machining (LECM)
by Guangxian Li, Zhikun Su, Tingan Zhao, Wei Wei and Songlin Ding
Metals 2024, 14(12), 1390; https://doi.org/10.3390/met14121390 - 4 Dec 2024
Viewed by 384
Abstract
Laser–electrochemical hybrid machining (LECM) is promising in the processing of thin-wall parts, which avoids problems such as the weak stiffness of structures and thermal defects. However, while most studies focus on precision machining via LECM, few investigate the potential of this technique in [...] Read more.
Laser–electrochemical hybrid machining (LECM) is promising in the processing of thin-wall parts, which avoids problems such as the weak stiffness of structures and thermal defects. However, while most studies focus on precision machining via LECM, few investigate the potential of this technique in macro-area processing. In this paper, the synergistic effects on the coupling of thermal field and electrochemical field on bulk material removal mechanisms in the LECM of additively manufactured Ti6Al4V are comprehensively analyzed experimentally and theoretically. According to the experimental results, LECM improved the material removal rate (MRR) by up to 28.6% compared to ECM. The induction of the laser increases local heating, accelerating the temperature rise of the electrolyte, eventually promoting the electrochemical reaction. The hydrogen bubble flow promotes overall heat convection between the electrode and workpiece, which facilitates the removal of the facial precipitates and increases the efficiency of electrochemical dissolution. Higher voltages and laser powers promote the formation of hydrogen bubble flow; meanwhile, they also aggravate laser energy scattering, limiting the overall machining efficiency. Additionally, laser irradiation causes the ablation and rupture of hydrogen bubbles, which weakens the bubble flow effect and ultimately decreases the material removal efficiency. This study reveals the underlying mechanisms of the joint effects of the laser field and electrical field in LECM, and the findings can provide valuable insights for the optimization of LECM parameters in industrial applications. Full article
Show Figures

Figure 1

Figure 1
<p>Schematic diagrams of three categories of LECM: (<b>a</b>) immersion; (<b>b</b>) jet; (<b>c</b>) tool electrode light-guiding.</p>
Full article ">Figure 2
<p>Ti6Al4V workpieces fabricated by selective laser melting.</p>
Full article ">Figure 3
<p>Experimental setups: (<b>a</b>) equipment and devices of the LECM system; (<b>b</b>) slot electrode; (<b>c</b>) electrolytic tank; (<b>d</b>) schematic diagram of the LECM process.</p>
Full article ">Figure 4
<p>Modelling of the LECM system in COMSOL: (<b>a</b>) section I is zone of electrolyte, section II is the electrode, and section III is the workpiece; (<b>b</b>) meshing of the model.</p>
Full article ">Figure 5
<p>Current graphs and turn times. (<b>a</b>) Current at different voltages and laser powers; (<b>b</b>) time required for the breakdown of passivation layers.</p>
Full article ">Figure 6
<p>Currents and their variations at different voltages. (<b>a</b>) Currents at different voltages; (<b>b</b>) increase of currents.</p>
Full article ">Figure 7
<p>MRRs and their variations. (<b>a</b>) MRRs of ECM and LECM at different voltages; (<b>b</b>) variation of MRRs with the induction of the laser at different voltages.</p>
Full article ">Figure 8
<p>Temperature distributions at different machining parameters: (<b>a</b>,<b>d</b>,<b>g</b>) are ECMs; (<b>b</b>,<b>e</b>,<b>h</b>) are LECM at 50 W; (<b>c</b>,<b>f</b>,<b>i</b>) are LECM at 100 W.</p>
Full article ">Figure 9
<p>Dynamic temperatures in the processes of ECM and LECM: (<b>a</b>) 10 V; (<b>b</b>) 15 V; (<b>c</b>) 20 V.</p>
Full article ">Figure 10
<p>Temperatures and their increases at different voltages. (<b>a</b>) Temperature values at different voltages; (<b>b</b>) increase of temperatures.</p>
Full article ">Figure 11
<p>Height distribution of machined surfaces: (<b>a</b>,<b>d</b>,<b>g</b>) are ECMs; (<b>b</b>,<b>e</b>,<b>h</b>) are LECM at 50 W; (<b>c</b>,<b>f</b>,<b>i</b>) are LECM at 100 W.</p>
Full article ">Figure 12
<p>Morphology of machined surfaces: (<b>a</b>,<b>d</b>,<b>g</b>) are ECMs; (<b>b</b>,<b>e</b>,<b>h</b>) are LECM at 50 W; (<b>c</b>,<b>f</b>,<b>i</b>) are LECM at 100 W.</p>
Full article ">Figure 13
<p>Precipitates and bubbles adhered to the surface of the workpiece after processing: (<b>a</b>,<b>d</b>,<b>g</b>) are ECMs; (<b>b</b>,<b>e</b>,<b>h</b>) are LECM at 50 W; (<b>c</b>,<b>f</b>,<b>i</b>) are LECM at 100 W.</p>
Full article ">Figure 14
<p>Surface micromorphology of machined workpieces (SEM 500×): (<b>a</b>) ECM 10 V; (<b>b</b>) ECM 15 V; (<b>c</b>) ECM 20 V; (<b>d</b>) LECM 15 V + 50 W.</p>
Full article ">Figure 15
<p>Electrochemical dissolution pits (SEM 3000×) on the surface of the workpiece after machining: (<b>a</b>) ECM (20 V); (<b>b</b>) LECM (10 V + 50 W).</p>
Full article ">Figure 16
<p>Recast layers (SEM 3000×) produced by LECM: (<b>a</b>) LECM (10 V + 50 W); (<b>b</b>) LECM (10 V + 100 W); (<b>c</b>) LECM (15 V + 50 W); and (<b>d</b>) LECM (15 V + 100 W).</p>
Full article ">Figure 17
<p>Schematic of the synergistic effects of laser and ECM on the enhancement of MRR.</p>
Full article ">Figure 18
<p>Simulation results of the electrolyte current density (time = 5 s). (<b>a</b>) ECM (10 V); (<b>b</b>) LECM (10 V + 50 W); (<b>c</b>) ECM (15 V); (<b>d</b>) LECM (15 V + 50 W); (<b>e</b>) ECM (20 V); (<b>f</b>) LECM (20 V + 50 W).</p>
Full article ">Figure 19
<p>Formation and flow of bubbles at the workpiece/electrode gap captured by a high-speed camera. (<b>a</b>) LECM (10 V + 100 W); (<b>b</b>) LECM (15 V + 100 W); (<b>c</b>) LECM (20 V + 100 W).</p>
Full article ">Figure 20
<p>Height distribution of the machined surface (30 V).</p>
Full article ">Figure 21
<p>Simulation results on temperature distributions of the electrolyte (time = 5 s). (<b>a</b>) ECM (10 V); (<b>b</b>) LECM (10 V + 50 W); (<b>c</b>) ECM (15 V); (<b>d</b>) LECM (15 V + 50 W); (<b>e</b>) ECM (20 V); (<b>f</b>) LECM (20 V + 50 W).</p>
Full article ">Figure 22
<p>Simulation results of the electrolyte current density on the workpiece surface. (<b>a</b>) 10 V; (<b>b</b>) 15 V; (<b>c</b>) 20 V.</p>
Full article ">Figure 23
<p>Elemental content of the recast layer. (<b>a</b>) LECM (10 V + 50 W); (<b>b</b>) LECM (10 V + 100 W); (<b>c</b>) LCM (50 W), (<b>d</b>) LCM (100 W).</p>
Full article ">
22 pages, 842 KiB  
Review
Current Treatment Methods in Hepatocellular Carcinoma
by Kamila Krupa, Marta Fudalej, Anna Cencelewicz-Lesikow, Anna Badowska-Kozakiewicz, Aleksandra Czerw and Andrzej Deptała
Cancers 2024, 16(23), 4059; https://doi.org/10.3390/cancers16234059 - 4 Dec 2024
Viewed by 551
Abstract
Hepatocellular carcinoma (HCC) is a prevalent malignant tumour worldwide. Depending on the stage of the tumour and liver function, a variety of treatment options are indicated. Traditional radiotherapy and chemotherapy are ineffective against HCC; however, the U.S. Food and Drug Administration (FDA) has [...] Read more.
Hepatocellular carcinoma (HCC) is a prevalent malignant tumour worldwide. Depending on the stage of the tumour and liver function, a variety of treatment options are indicated. Traditional radiotherapy and chemotherapy are ineffective against HCC; however, the U.S. Food and Drug Administration (FDA) has approved radiofrequency ablation (RFA), surgical resection, and transarterial chemoembolization (TACE) for advanced HCC. On the other hand, liver transplantation is recommended in the early stages of the disease. Tyrosine kinase inhibitors (TKIs) like lenvatinib and sorafenib, immunotherapy and anti-angiogenesis therapy, including pembrolizumab, bevacizumab, tremelimumab, durvalumab, camrelizumab, and atezolizumab, are other treatment options for advanced HCC. Moreover, to maximize outcomes for patients with HCC, the combination of immune checkpoint inhibitors (ICIs) along with targeted therapies or local ablative therapy is being investigated. This review elaborates on the current status of HCC treatment, outlining the most recent clinical study results and novel approaches. Full article
(This article belongs to the Section Cancer Therapy)
Show Figures

Figure 1

Figure 1
<p>Current treatment methods in hepatocellular carcinoma. Abbreviations: LT—Liver Transplantation, TACE—Transarterial Chemoembolization, TKIs—Tyrosine Kinase Inhibitors, ICIs—Immune Checkpoint Inhibitors, BCLC—Barcelona Clinic Liver Cancer staging system.</p>
Full article ">
Back to TopTop