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23 pages, 5693 KiB  
Article
Sea Surface Wind Speed Retrieval Using Gaofen-3-02 SAR Full Polarization Data
by Kuo Zhang, Yuxin Hu, Junxin Yang and Xiaochen Wang
Remote Sens. 2025, 17(4), 591; https://doi.org/10.3390/rs17040591 - 9 Feb 2025
Viewed by 272
Abstract
The primary payload onboard the Gaofen-3-02 (GF3-02) satellite is a C-band Synthetic Aperture Radar (SAR) capable of achieving a maximum resolution of 1 m. This instrument is critical to monitor the marine environment, particularly for tracking sea surface wind speeds, an important marine [...] Read more.
The primary payload onboard the Gaofen-3-02 (GF3-02) satellite is a C-band Synthetic Aperture Radar (SAR) capable of achieving a maximum resolution of 1 m. This instrument is critical to monitor the marine environment, particularly for tracking sea surface wind speeds, an important marine environmental parameter. In this study, we utilized 192 sets of GF3-02 SAR data, acquired in Quad-Polarization Strip I (QPSI) mode in March 2022, to retrieve sea surface wind speeds. Prior to wind speed retrieval for vertical-vertical (VV) polarization, radiometric calibration accuracy was analyzed, yielding good performance. The results showed a bias and root mean square errors (RMSEs) of 0.02 m/s and 1.36 m/s, respectively, when compared to the European Centre for Medium-Range Weather Forecasts (ECMWF) Reanalysis V5 (ERA5) data. For horizontal–horizontal (HH) polarization, two types of polarization ratio (PR) models were introduced based on the GF3-02 SAR data. Combining these refitted PR models with CMOD5.N, the results for HH polarization exhibited a bias of −0.18 m/s and an RMSE of 1.25 m/s in comparison to the ERA5 data. Regarding vertical–horizontal (VH) polarization, two linear models based on both measured normalized radar cross sections (NRCSs) and denoised NRCSs were developed. The findings indicate that denoising significantly enhances the accuracy of wind speed measurements for VH polarization when dealing with low wind speeds. When compared against buoy data, the wind speed retrieval results demonstrated a bias of 0.23 m/s and an RMSE of 1.77 m/s. Finally, a comparative analysis of the above retrieval results across all three polarizations was conducted to further understand their respective performances. Full article
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<p>The geolocations of the acquired QPSI mode data.</p>
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<p>The flow chart of the comparison process between ERA5 data and SAR data.</p>
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<p>The geolocations of the matched scene–buoy pairs. (<b>a</b>) Station 46002; (<b>b</b>) Station 51000; (<b>c</b>) Station 51004. The buoys are denoted by black dots. The red boxes represent the scenes that match the buoys.</p>
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<p>The flow chart of the comparison process between buoy data and SAR data.</p>
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<p>The relationship between the absolute bias of NRCS at a wind speed bias of 0.5 m/s and wind speed for different incidence angles and relative wind directions.</p>
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<p>The disparity between the simulated NRCSs and the actual measured NRCSs in the subscene. The red horizontal lines indicate the threshold, which is 1.43 dB.</p>
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<p>Evaluating the consistency between the wind speeds derived from VV polarization data and the corresponding ERA5 wind speeds. (<b>a</b>) Scatter diagram; (<b>b</b>) histogram.</p>
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<p>The dependence of the PR in a linear unit on incidence angle and relative wind direction. (<b>a</b>) PR and incidence angle (<b>b</b>) PR and relative wind direction.</p>
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<p>Comparisons of the three refitted models and the GF3-02 SAR data.</p>
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<p>Comparisons of the converted NRCS from HH polarization and the measured NRCS for VV polarization. (<b>a</b>) Refitted Elfouhaily model. (<b>b</b>) Refitted Thompson model. (<b>c</b>) Refitted Mouche model. (<b>d</b>) Refitted Mouche–azimuth model.</p>
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<p>Examinations of the wind speeds obtained from HH polarization against the ERA5 wind speeds. Scatter diagram: (<b>a</b>) Refitted Elfouhaily model. (<b>b</b>) Refitted Thompson model. (<b>c</b>) Refitted Mouche model. (<b>d</b>) Refitted Mouche–azimuth model. Histogram: (<b>e</b>) Refitted Elfouhaily model. (<b>f</b>) Refitted Thompson model. (<b>g</b>) Refitted Mouche model. (<b>h</b>) Refitted Mouche–azimuth model.</p>
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<p>Examinations of the wind speeds obtained from HH polarization against the ERA5 wind speeds. Scatter diagram: (<b>a</b>) Refitted Elfouhaily model. (<b>b</b>) Refitted Thompson model. (<b>c</b>) Refitted Mouche model. (<b>d</b>) Refitted Mouche–azimuth model. Histogram: (<b>e</b>) Refitted Elfouhaily model. (<b>f</b>) Refitted Thompson model. (<b>g</b>) Refitted Mouche model. (<b>h</b>) Refitted Mouche–azimuth model.</p>
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<p>Scatter plots of the NRCS for VH polarization and the matched ERA5 wind speeds. (<b>a</b>) Measured NRCS. (<b>b</b>) Denoised NRCS.</p>
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<p>Examinations of the wind speeds retrieved from VH polarization in comparison with the ERA5 wind speeds. Scatter diagram: (<b>a</b>) Measured NRCS. (<b>b</b>) Denoised NRCS. Histogram: (<b>c</b>) Measured NRCS. (<b>d</b>) Denoised NRCS.</p>
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<p>Examinations of the wind speeds retrieved from VH polarization in comparison with the buoy wind speeds. (<b>a</b>) Measured NRCS. (<b>b</b>) Denoised NRCS.</p>
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<p>The distribution map of retrieved wind speed and wind speed bias compared with ERA5 data for the typhoon Hinnamnor. (<b>a</b>) Retrieved wind speed. (<b>b</b>) Wind speed bias.</p>
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<p>The distribution of wind speed retrieval results of VV polarization, HH polarization and VH polarization.</p>
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<p>The spatial distribution of wind speed retrieval results (<b>a</b>) VV polarization. (<b>b</b>) HH polarization. (<b>c</b>) VH polarization.</p>
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<p>The spatial distribution of retrieved wind speed bias compared with ERA5 wind speed. (<b>a</b>) VV polarization. (<b>b</b>) HH polarization. (<b>c</b>) VH polarization.</p>
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15 pages, 3018 KiB  
Article
Withaferin A Attenuates Muscle Cachexia Induced by Angiotensin II Through Regulating Pathways Activated by Angiotensin II
by Sham S. Kakar, Vasa Vemuri and Mariusz Z. Ratajczak
Cells 2025, 14(4), 244; https://doi.org/10.3390/cells14040244 - 8 Feb 2025
Viewed by 347
Abstract
Cachexia is a multifactorial syndrome characterized by severe muscle wasting and is a debilitating condition frequently associated with cancer. Previous studies from our group revealed that withaferin A (WFA), a steroidal lactone, mitigated muscle cachexia induced by ovarian tumors in NSG mice. However, [...] Read more.
Cachexia is a multifactorial syndrome characterized by severe muscle wasting and is a debilitating condition frequently associated with cancer. Previous studies from our group revealed that withaferin A (WFA), a steroidal lactone, mitigated muscle cachexia induced by ovarian tumors in NSG mice. However, it remains unclear whether WFA’s protective effects are direct or secondary to its antitumor properties. We developed a cachectic model through continuous angiotensin II (Ang II) infusion in C57BL/6 mice to address this issue. Ang II infusion resulted in profound muscle atrophy, evidenced by significant reductions in grip strength and in the TA, GA, and GF muscle mass. Molecular analyses indicated elevated expression of inflammatory cytokines (TNFα, IL-6, MIP-2, IL-18, IL-1β), NLRP3 inflammasome, and genes associated with the UPS (MuRF1, MAFBx) and autophagy pathways (Bacl1, LC3B), along with suppression of anti-inflammatory heme oxygenase-1 (HO-1) and myogenic regulators (Pax7, Myod1). Strikingly, WFA treatment reversed these pathological changes, restoring muscle mass, strength, and molecular markers to near-normal levels. These findings demonstrate that WFA exerts direct anti-cachectic effects by targeting key inflammatory and atrophic pathways in skeletal muscle, highlighting its potential as a novel therapeutic agent for cachexia management. Full article
(This article belongs to the Section Cells of the Cardiovascular System)
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<p>Withaferin A effectively restores grip strength impaired by Ang II. The normalized average grip strength, including (<b>A</b>) forelimb and (<b>B</b>) total limb strength, was assessed across Ang II-infused vehicle-treated groups and saline-infused controls, as well as the WFA-treated group (4 mg/kg) at baseline (week 0) and subsequent time points (week 1, week 2, and week 4) after osmotic pump implantation (N = 5 per group). Statistical analysis utilizing two-way ANOVA and Tukey’s multiple comparison tests revealed significant differences between groups. Statistical significance is indicated as follows: * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, **** <span class="html-italic">p</span> &lt; 0.0001, comparing Ang II-infused vehicle-treated groups to saline-infused controls. Additionally, <sup>@@</sup> <span class="html-italic">p</span> &lt; 0.01, <sup>@@@</sup> <span class="html-italic">p</span> &lt; 0.001 denote significant differences between Ang II-infused WFA-treated groups and Ang II-infused vehicle-treated groups. N = 5/group.</p>
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<p>Withaferin A reduces muscle weight loss caused by Ang II infusion. The graph displays the wet weights of the tibialis anterior (TA), gastrocnemius (GA), and quadriceps femoris (QF) muscles, normalized to initial body weight (IBW) to account for baseline variability among experimental groups (N = 5 per group). Ang II infusion significantly decreased normalized muscle weights compared to the saline-infused vehicle-treated controls. Treatment with withaferin A (WFA) significantly recovered muscle mass in Ang II-infused animals and increased muscle weights in saline-infused animals. Statistical significance was assessed using two-way ANOVA followed by Tukey’s multiple comparison post hoc analysis. Symbols denote: ** <span class="html-italic">p</span> &lt; 0.01; *** <span class="html-italic">p</span> &lt; 0.001; **** <span class="html-italic">p</span> &lt; 0.0001 for comparisons between Ang II-infused vehicle-treated and saline-infused groups, and <sup>@@</sup> <span class="html-italic">p</span> &lt; 0.01; <sup>@@@</sup> <span class="html-italic">p</span> &lt; 0.001; <sup>@@@@</sup> <span class="html-italic">p</span> &lt; 0.0001 for comparisons between Ang II-infused WFA-treated and Ang II-infused vehicle-treated groups.</p>
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<p>Withaferin A restores myofibrillar integrity in skeletal muscle. (<b>A</b>) Representative transverse sections of the tibialis anterior (TA) muscle, stained with hematoxylin and eosin (H&amp;E), highlight structural differences among the treatment groups. The images illustrate how withaferin A affects muscle architecture, showing visible differences in myofiber size and organization. Insets provide magnified views of selected areas from the larger images to effectively showcase the cellular and tissue-level changes. Scale bar = 50 μm. (<b>B</b>) Quantitative analysis of myofiber cross-sectional area (CSA) clarifies the impact of withaferin A treatment on myofiber size. (<b>C</b>) Minimal Feret’s diameter measurements further quantify myofiber integrity, assessing the structural alterations in the TA muscle. Together, these parameters reveal the restorative effects of withaferin A on muscle structure and integrity. Data represent a sample size of N = 5 per group. Statistical analysis indicates significance at ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001, and **** <span class="html-italic">p</span> &lt; 0.0001, marking significant differences between Ang II-infused groups and their saline-infused counterparts. Additionally, <sup>@@@@</sup> <span class="html-italic">p</span> &lt; 0.0001 highlights a significant difference between Ang II-infused, WFA-treated groups and Ang II-infused vehicle-treated groups. Statistical assessments were conducted using two-way ANOVA followed by Tukey’s multiple comparison test for post hoc analysis.</p>
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<p>Withaferin A influences the expression of inflammatory cytokines in gastrocnemius (GA) muscles induced by Ang II. Relative mRNA expression levels of pro-inflammatory cytokines (TNF-α, IL-6, MIP-2, IL-18, IL-1β) and the anti-inflammatory cytokine HO-1 in GA muscles were measured. Data are presented as the mean ± SD, with individual data points shown as black circles (N = 5 per group). Statistical significance was assessed using two-way ANOVA followed by Tukey’s multiple comparison test. * <span class="html-italic">p</span> &lt; 0.05; ** <span class="html-italic">p</span> &lt; 0.01; *** <span class="html-italic">p</span> &lt; 0.001; **** <span class="html-italic">p</span> &lt; 0.0001 indicate significant differences between the Ang II-infused and saline-infused vehicle-treated groups. <sup>@@</sup> <span class="html-italic">p</span> &lt; 0.01, <sup>@@@</sup> <span class="html-italic">p</span> &lt; 0.001, <sup>@@@@</sup> <span class="html-italic">p</span> &lt; 0.0001 denote significant differences between the Ang II-infused WFA-treated group and the Ang II-infused vehicle-treated group.</p>
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<p>Withaferin A reverses Ang II-induced activation of the NLRP3 inflammasome. Mice were infused with Ang II, as outlined in the Materials and Methods section. After 4 weeks of continuous Ang II infusion, gastrocnemius muscle tissues were collected from each of the four experimental groups. The mRNA expression levels of NLRP3 and Caspase-1 were quantified using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Data are presented as mean ± SD (n = 5 per group). Statistical significance was assessed by comparing the Ang II-infused groups to the saline-infused controls, with ** <span class="html-italic">p</span> &lt; 0.01 and **** <span class="html-italic">p</span> &lt; 0.0001. Additionally, <sup>@@@@</sup> <span class="html-italic">p</span> &lt; 0.0001 denotes a significant difference between the Ang II-infused vehicle-treated and Ang II-infused WFA-treated groups.</p>
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<p>Withaferin A downregulates the activation of the ubiquitin–proteasome system (UPS) and autophagy-related genes. This figure displays relative mRNA levels of key markers linked to the UPS and autophagy in gastrocnemius (GA) muscles from both saline-infused and Ang II-infused groups. Data are presented as mean ± SD, with N = 5 per group. Statistical significance was assessed using two-way ANOVA followed by Tukey’s multiple comparison post hoc analysis. * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, **** <span class="html-italic">p</span> &lt; 0.0001 indicate significant differences between Ang II-infused and saline-infused vehicle-treated groups. Additionally, <sup>@@@@</sup> <span class="html-italic">p</span> &lt; 0.0001 shows significant differences from the corresponding values of the Ang II-infused WFA-treated group compared to the Ang II-infused vehicle-treated group.</p>
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<p>Withaferin A (WFA) reduces the expression of satellite cell-related genes in response to Ang II infusion. mRNA levels of Pax7 and Myod1 were measured in gastrocnemius (GA) muscles from saline-infused and Ang II-infused animals, both with and without WFA treatment. N = 5 per group. Data are presented as mean ± SD. * <span class="html-italic">p</span> &lt; 0.05 and **** <span class="html-italic">p</span> &lt; 0.0001 indicate significant differences from the corresponding value of the Ang II-infused vehicle-treated group, as determined by two-way ANOVA followed by Tukey’s multiple comparison test. <sup>@@@@</sup> <span class="html-italic">p</span> &lt; 0.0001 indicates a significant difference comparing the Ang II-infused vehicle-treated group to the Ang II-infused WFA-treated group.</p>
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18 pages, 5396 KiB  
Article
A Road Extraction Algorithm for the Guided Fusion of Spatial and Channel Features from Multi-Spectral Images
by Lin Gao, Yongqi Zhang, Aolin Jiao and Lincong Zhang
Appl. Sci. 2025, 15(4), 1684; https://doi.org/10.3390/app15041684 - 7 Feb 2025
Viewed by 370
Abstract
In the road extraction task, for the problem of low utilization of spectral features in high-resolution remote sensing images, we propose a Multi-spectral image-guided fusion of Spatial and Channel Features for road extraction algorithm (SC-FMNet). The method is designed with a two-branch input [...] Read more.
In the road extraction task, for the problem of low utilization of spectral features in high-resolution remote sensing images, we propose a Multi-spectral image-guided fusion of Spatial and Channel Features for road extraction algorithm (SC-FMNet). The method is designed with a two-branch input network structure including Multi-spectral image and fused image branches. Based on the original MSNet model, the Spatial and Channel Reconstruction Convolution (SCConv) module is introduced in the coding part in each of the two branches. In addition, a Spatially Adaptive Feature Modulation Mechanism (SAFMM) module is introduced into the decoding structure. The experimental results in the GF2-FC and CHN6-CUG road datasets show that the method can better extract the road information and improve the accuracy of road segmentation, which verify the effectiveness of SC-FMNet. Full article
(This article belongs to the Special Issue Intelligent Computing and Remote Sensing—2nd Edition)
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<p>SC-FMNet network structure diagram.</p>
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<p>SCConv structure diagram.</p>
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<p>SRU structure diagram.</p>
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<p>CRU structure diagram.</p>
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<p>SAFMM structure diagram.</p>
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<p>SAFM structure diagram.</p>
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<p>The ablation effect was extracted from the GF2-FC dataset.</p>
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<p>The ablation effect was extracted from the CHN6-CUG dataset.</p>
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<p>Comparison chart of different network test results in the GF2-FC dataset.</p>
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<p>Comparison of network test results in the CHN6-CUG dataset.</p>
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21 pages, 1312 KiB  
Article
Impact of Green Finance on Chinese Urban Land Green Use Efficiency: An Empirical Study Based on a Quasinatural Experiment
by Fen Wang, Haikuo Zhang and Jingjie Zhou
Land 2025, 14(2), 332; https://doi.org/10.3390/land14020332 - 6 Feb 2025
Viewed by 327
Abstract
To examine the impact of green finance (GF) on urban land green use efficiency (LGUE), we treat the Green Finance Reform and Innovation Pilot Zone (GFRIPZ) policy, implemented in 2017, as quasi-natural experiment. The results from a multi-period difference-in-difference model show that GF [...] Read more.
To examine the impact of green finance (GF) on urban land green use efficiency (LGUE), we treat the Green Finance Reform and Innovation Pilot Zone (GFRIPZ) policy, implemented in 2017, as quasi-natural experiment. The results from a multi-period difference-in-difference model show that GF contributes to improving urban LGUE. This conclusion is validated further by a generalized random forest model. The mechanism analysis demonstrates that GF enhances LGUE through the effects of green technological innovation, industrial upgrading, and public green behavior. The moderation analysis further reveals that artificial intelligence can amplify the positive impact of GF on LGUE. The heterogeneity results show that the positive relationship between GF and LGUE is more pronounced in midwestern cities, non-resource-based cities, and cities with a high level of financial development. Therefore, it is essential to expand the GF pilot program in a structured manner and establish a coordinated mechanism to promote LGUE improvement through GF in different regions, thereby enhancing financial service efficiency for the real economy. Full article
(This article belongs to the Section Land Environmental and Policy Impact Assessment)
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<p>Research framework.</p>
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<p>Parallel trend.</p>
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<p>Placebo test.</p>
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23 pages, 6481 KiB  
Article
Nonlinear Quantization Method of SAR Images with SNR Enhancement and Segmentation Strategy Guidance
by Zijian Yao, Linlin Fang, Junxin Yang and Lihua Zhong
Remote Sens. 2025, 17(3), 557; https://doi.org/10.3390/rs17030557 - 6 Feb 2025
Viewed by 339
Abstract
The quantization process of synthetic aperture radar (SAR) images faces significant challenges due to their high dynamic range, resulting in notable quantization distortion. This not only degrades the visual quality of the quantized images but also severely impacts the accuracy of image interpretation. [...] Read more.
The quantization process of synthetic aperture radar (SAR) images faces significant challenges due to their high dynamic range, resulting in notable quantization distortion. This not only degrades the visual quality of the quantized images but also severely impacts the accuracy of image interpretation. To mitigate the distortion caused by uniform quantization and enhance visual quality, this paper introduced a novel nonlinear quantization framework via signal-to-noise ratio (SNR) enhancement and segmentation strategy guidance. This framework introduces guiding information to improve quantization performance in weak scattering regions. A histogram adjustment method is developed to incorporate the spatial information of SAR images into the quantization process to enhance the quantization performance, specifically within weak scattering regions. Additionally, the optimal quantizer is improved by refining the SNR distribution across quantization units, addressing imbalances in their allocation. Experimental results based on Gaofen-3 (GF-3) satellite data demonstrate that the proposed algorithm approaches the global quantization performance of optimal quantizers while achieving superior local quantization performance compared to existing methods. Full article
(This article belongs to the Section Remote Sensing Image Processing)
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<p>Overview of the nonlinear quantization method framework.</p>
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<p>Overall framework of the proposed method.</p>
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<p>Results of the dynamic range of SAR images and histogram proportion. (<b>a</b>) Dynamic range results of SAR images for different land cover types. (<b>b</b>) Histogram proportion of SAR images for different land cover types in the [1:1000] quantization level range.</p>
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<p>Extraction results of sparse strong scattering points. The orange regions indicate the distribution of strong scattering points, while the blue histograms represent the image histogram. (<b>a</b>) Histogram of the land scene. (<b>b</b>) Histogram of the coast scene. (<b>c</b>) Histogram of the ocean scene.</p>
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<p>Segmentation experiment results. (<b>a</b>,<b>d</b>,<b>g</b>) Original quantized images. (<b>b</b>,<b>e</b>,<b>h</b>) Histogram segmentation results. (<b>c</b>,<b>f</b>,<b>i</b>) Morphological transformation processing results.</p>
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<p>Histogram of fine-tuning experimental results. (<b>a</b>–<b>f</b>) Histogram fusion results of six different SAR coast scene images.</p>
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<p>Image quantization experimental results.</p>
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<p>Local effects of nonlinear quantization experiment. (<b>a</b>) Original image, with the selected weak scattering area indicated by a red box. (<b>b</b>) Uniform quantization. (<b>c</b>) Histogram equalization. (<b>d</b>) Logarithmic quantization. (<b>e</b>) Optimal quantization. (<b>f</b>) The algorithm proposed in this paper.</p>
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<p>Quantization distortion experimental results for each quantization levels.</p>
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<p>Q–SNR experimental results for each quantization level.</p>
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<p>Q–SNR experimental results at the quantization levels of [0, 3000].</p>
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<p>Ground truth and clustering results with different methods. (<b>a</b>) Original image. (<b>b</b>) Ground truth label. (<b>c</b>) Histogram equalization clustering. (<b>d</b>) Log quantization clustering. (<b>e</b>) Optimal quantization clustering. (<b>f</b>) Proposed method clustering.</p>
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<p>Comparisons of <math display="inline"><semantics> <msub> <mi>F</mi> <mn>1</mn> </msub> </semantics></math> score in different binary classifications with different methods. (<b>a</b>) <math display="inline"><semantics> <msub> <mi>F</mi> <mn>1</mn> </msub> </semantics></math> score curve of land classification. (<b>b</b>) <math display="inline"><semantics> <msub> <mi>F</mi> <mn>1</mn> </msub> </semantics></math> score curve of ocean classification.</p>
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20 pages, 4669 KiB  
Article
Monitoring Mangrove Phenology Based on Gap Filling and Spatiotemporal Fusion: An Optimized Mangrove Phenology Extraction Approach (OMPEA)
by Yu Hong, Runfa Zhou, Jinfu Liu, Xiang Que, Bo Chen, Ke Chen, Zhongsheng He and Guanmin Huang
Remote Sens. 2025, 17(3), 549; https://doi.org/10.3390/rs17030549 - 6 Feb 2025
Viewed by 386
Abstract
Monitoring mangrove phenology requires frequent, high-resolution remote sensing data, yet satellite imagery often suffers from coarse resolution and cloud interference. Traditional methods, such as denoising and spatiotemporal fusion, faced limitations: denoising algorithms usually enhance temporal resolution without improving spatial quality, while spatiotemporal fusion [...] Read more.
Monitoring mangrove phenology requires frequent, high-resolution remote sensing data, yet satellite imagery often suffers from coarse resolution and cloud interference. Traditional methods, such as denoising and spatiotemporal fusion, faced limitations: denoising algorithms usually enhance temporal resolution without improving spatial quality, while spatiotemporal fusion models struggle with prolonged data gaps and heavy noise. This study proposes an optimized mangrove phenology extraction approach (OMPEA), which integrates Landsat and MODIS data with a denoising algorithm (e.g., Gap Filling and Savitzky–Golay filtering, GF–SG) and a spatiotemporal fusion model (e.g., Enhanced Spatial and Temporal Adaptive Reflectance Fusion Model, ESTARFM). The key of OMPEA is that GF–SG algorithm filled data gaps from cloud cover and satellite transit gaps, providing high-quality input to ESTARFM and improving its accuracy of NDVI imagery reconstruction in mangrove phenology extraction. By conducting experiments on the GEE platform, OMPEA generates 1-day, 30 m NDVI imagery, from which phenological parameters (i.e., the start (SoS), end (EoS), length (LoS), and peak (PoS) of the growing season) are derived using the maximum separation (MS) method. Validation in four mangrove areas along the coastal China shows that OMPEA significantly improves the potential to capture mangrove phenology in the presence of incomplete data. The OMPEA significantly increased usable data, adding 7–33 Landsat images and 318–415 MODIS images per region. The generated NDVI series exhibits strong spatiotemporal consistency with original data (R2: 0.788–0.998, RMSE: 0.007–0.253) and revealed earlier SoS and longer LoS at lower latitudes. Cross-correlation analysis showed a 2–3 month lagged effects of temperature on mangroves’ growth, with precipitation having minimal impact. The proposed OMPEA improves the possibility of capturing mangrove phenology under non-continuous and low-resolution data, providing valuable insights for large-scale and long-term mangrove conservation and management. Full article
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<p>Map of study area: (<b>a</b>) the overall distribution of study area; (<b>b1</b>–<b>b4</b>) Zhangjiangkou National Mangrove Nature Reserve (ZNR) in Fujian Province, Qi’ao Island Provincial Nature Reserve (QPR) in Guangdong Province, Beilun Estuary National Nature Reserve (BNR) in Guangxi Province, and Dongzhaigang National Mangrove Nature Reserve (DNR) in Hainan Province.</p>
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<p>Workflow of mangrove phenology extraction based on OMPEA.</p>
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<p>Landsat 8 NDVI (16-day 30 m) and denoised Landsat NDVI (16-day 30 m) generated by OMPEA. Gray pixel indicates pixel with no data.</p>
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<p>MODIS NDVI (1-day 500 m) and denoised MODIS NDVI (1-day 30 m) generated by OMPEA. Gray pixel indicates pixel with no data.</p>
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<p>The OMPEA-generated fused NDVI imagery. Gray pixel indicates pixel with no data.</p>
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<p>Scatter density plots and marginal histograms of fused NDVI and denoised Landsat NDVI.</p>
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<p>Composite scatter plots and line plots of various NDVI time series.</p>
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<p>Fused NDVI time-series curve and phenological parameters.</p>
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<p>Boxplots of mangrove phenological parameters.</p>
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<p>The time-series curves for fused NDVI, precipitation, temperature, and their lagged time-series curves with corresponding lag days.</p>
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<p>The OMPEA-generated fused NDVI in QPR from 17 January 2020 to 24 March 2021. (<b>a</b>) Description of denoised Landsat 8 NDVI in a full-time range. (<b>b</b>) Description of denoised Landsat 8 NDVI across three different time ranges, (<b>c</b>,<b>d</b>) is fused NDVI that using (<b>a</b>,<b>b</b>) as inputs, respectively. Gray pixel indicates pixel with no data.</p>
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22 pages, 2496 KiB  
Article
Positioning Technology Without Ground Control Points for Spaceborne Synthetic Aperture Radar Images Using Rational Polynomial Coefficient Model Considering Atmospheric Delay
by Doudou Hu, Chunquan Cheng, Shucheng Yang and Chengxi Hu
Appl. Sci. 2025, 15(3), 1615; https://doi.org/10.3390/app15031615 - 5 Feb 2025
Viewed by 361
Abstract
This study addresses the issue of atmospheric delay correction for the rational polynomial coefficient (RPC) model associated with spaceborne synthetic aperture radar (SAR) imagery under conditions lacking ephemeris data, proposing a novel approach to enhance the geometric positioning accuracy of RPC models. A [...] Read more.
This study addresses the issue of atmospheric delay correction for the rational polynomial coefficient (RPC) model associated with spaceborne synthetic aperture radar (SAR) imagery under conditions lacking ephemeris data, proposing a novel approach to enhance the geometric positioning accuracy of RPC models. A satellite position inversion method based on the vector-autonomous intersection technique was developed, incorporating ionospheric delay and neutral atmospheric delay models to derive atmospheric delay errors. Additionally, an RPC model reconstruction approach, which integrates atmospheric correction, is proposed. Validation experiments using GF-3 satellite imagery demonstrated that the atmospheric delay values obtained by this method differed by only 0.0001 m from those derived using the traditional ephemeris-based approach, a negligible difference. The method also exhibited high robustness in long-strip imagery. The reconstructed RPC parameters improved image-space accuracy by 18–44% and object-space accuracy by 19–32%. The results indicate that this approach can fully replace traditional ephemeris-based methods for atmospheric delay extraction under ephemeris-free conditions, significantly enhancing the geometric positioning accuracy of SAR imagery RPC models, with substantial application value and development potential. Full article
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<p>Radar LOS vector inversion.</p>
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<p>Satellite position inversion.</p>
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<p>Ionospheric single-layer model.</p>
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<p>GF-3 data. (<b>a</b>) area1; (<b>b</b>) area2. The red box indicates the data of rail lift and the blue box indicates the data of rail descent.</p>
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<p>Satellite position inversion accuracy. (<b>a</b>) Satellite position error; (<b>b</b>) slant-range error.</p>
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<p>Electron density and specific humidity in the zenith direction. (<b>a</b>) Electron density in the zenith direction; (<b>b</b>) specific humidity in the zenith direction.</p>
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<p>Atmospheric delamination delay. (<b>a</b>) gf1 ionospheric delay; (<b>b</b>) gf7 ionospheric delay; (<b>c</b>) gf1 neutral atmospheric delay; (<b>d</b>) gf7 neutral atmospheric delay.</p>
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<p>Atmospheric delay.</p>
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<p>SAR image positioning accuracy. (<b>a</b>) Image-space accuracy; (<b>b</b>) object-space accuracy.</p>
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24 pages, 8896 KiB  
Article
A Prediction of Estuary Wetland Vegetation with Satellite Images
by Min Yang, Bin Guo, Ning Gao, Yang Yu, Xiaoli Song and Yanfeng Gu
J. Mar. Sci. Eng. 2025, 13(2), 287; https://doi.org/10.3390/jmse13020287 - 4 Feb 2025
Viewed by 416
Abstract
Estuarine wetlands are the transition zone between marine, freshwater, and terrestrial ecosystems and are more ecologically fragile. In recent years, the spread of exotic vegetation, specifically Spartina alterniflora, in the Yellow River estuary wetlands has significantly encroached upon the habitats of native [...] Read more.
Estuarine wetlands are the transition zone between marine, freshwater, and terrestrial ecosystems and are more ecologically fragile. In recent years, the spread of exotic vegetation, specifically Spartina alterniflora, in the Yellow River estuary wetlands has significantly encroached upon the habitats of native species such as Phragmites australis, Suaeda glauca Bunge, and Tamarix chinensis Lour. With advances in land prediction modeling, predicting wetland vegetation distribution can aid management and decision-making for ecological restoration. We selected the core area as the study object and coupled the hydrological model MIKE 21 with the PLUS model to predict the potential future distribution of invasive and dominant species in the region. (1) Based on the fine classification results from satellite images of GF1/G2/G5, we gained an understanding of the changes in wetland vegetation types in the core area of the reserve in 2018 and 2020. (2) Using public data such as ERA5 and GEO as input for basic environmental data, using MIKE 21 to provide high-spatial-resolution hydrodynamic parameters for the PLUS model as an environmental driver, we modeled the spatial distribution of various wetland vegetation in the Yellow River estuary wetland in Dongying under different artificial restoration measures. (3) We predicted the 2022 distribution of typical vegetation in the region, used the classification results of GF6 as the actual distribution, compared the spatial distribution with the actual distribution, and obtained a kappa coefficient of 0.78; the predicted values of the model are highly consistent with the true values. This study combines the fine classification results of vegetation based on hyperspectral remote sensing, the construction of a coupled model, and the prediction effect of typical species, providing a reference for constructing and optimizing the vegetation prediction model of estuarine wetlands. It also allows scientific and effective decision-making for the management of ecological restoration of delta wetlands. Full article
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<p>Research area-the Yellow River estuary wetlands.</p>
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<p>Schematic diagram of the coupling models.</p>
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<p>Grid range of MIKE 21.</p>
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<p>Schematic diagram of the ecological restoration area of the Yellow River estuary delta.</p>
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<p>The processing flow of MIKE 21-PLUS coupling model in artificial ecological restoration measures.</p>
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<p>Hydrodynamic simulation of MIKE21 model. (<b>a</b>) Hydrodynamic simulation within the restoration area; (<b>b</b>) current velocity without tidal creek; and (<b>c</b>) flow velocity under tidal ditch conditions.</p>
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<p>Changes in the distribution of features in the Yellow River estuary, 2018–2022.</p>
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<p>Model realization process for mowing and replanting.</p>
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<p>Comparison of observed and modeled salinity in Laizhou Bay. (Salinity data from the environmental survey of Laizhou Bay in August 2020 by Beihai Bureau of the Ministry of Natural Resources).</p>
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<p>Comparison of simulation results based on MIKE 21-PLUS with actual results.</p>
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<p>Comparison of simulation results between natural and artificial restoration scenarios in the restoration area.</p>
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<p>Environmental drivers of the evolution of the distribution of <span class="html-italic">Spartina alterniflora</span>, <span class="html-italic">Suaeda glauca Bunge</span>, and <span class="html-italic">Reed</span> (<span class="html-italic">Phragmites australis</span>).</p>
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<p>Comparison of <span class="html-italic">F</span><sub>1</sub>-<span class="html-italic">score</span> for simulating vegetation distribution in the Yellow River estuary wetland in 2022 using MIKE21-PLUS and PLUS.</p>
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16 pages, 2376 KiB  
Article
Distinct Impact of Processing on Cross-Order Cry1I Insecticidal Activity
by Dafne Toledo, Yolanda Bel, Stefanie Menezes de Moura, Juan Luis Jurat-Fuentes, Maria Fatima Grossi de Sa, Aida Robles-Fort and Baltasar Escriche
Toxins 2025, 17(2), 67; https://doi.org/10.3390/toxins17020067 - 3 Feb 2025
Viewed by 534
Abstract
The insecticidal Cry proteins from Bacillus thuringiensis are used in biopesticides or transgenic crops for pest control. The Cry1I protein family has unique characteristics of being produced during the vegetative rather than sporulation phase, its protoxins forming dimers in solution, and exhibiting dual [...] Read more.
The insecticidal Cry proteins from Bacillus thuringiensis are used in biopesticides or transgenic crops for pest control. The Cry1I protein family has unique characteristics of being produced during the vegetative rather than sporulation phase, its protoxins forming dimers in solution, and exhibiting dual toxicity against lepidopteran and coleopteran pests. The Cry1Ia protoxin undergoes sequential proteolysis from the N- and C-terminal ends, producing intermediate forms with insecticidal activity, while in some cases, the fully processed toxin is inactive. We investigated the oligomerization and toxicity of Cry1Ia intermediate forms generated through trypsinization (T-Int) and larval gut fluid (GF-Int) treatments, as well as the fully trypsinized protein (toxin). Heterologously expressed intermediate forms assembled into oligomers and showed similar toxicity to Cry1Ia protoxin against Ostrinia nubilalis (European corn borer) larvae, while the toxin form was ~30 times less toxic. In contrast, bioassays with Leptinotarsa decemlineata (Colorado potato beetle) larvae did not show significant differences in toxicity among Cry1Ia protoxin, T-Int, GF-Int, and fully processed toxin. These results suggest that the Cry1I mode of action differs by insect order, with N-terminal cleavage affecting toxicity against lepidopteran but not coleopteran larvae. This knowledge is essential for designing pest control strategies using Cry1I insecticidal proteins. Full article
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<p>Schematic representation of the Cry1Ia38 protoxin, trypsin intermediate (T-Int), gut fluid intermediate (GF-Int), and the fully processed (Toxin) domain features and cleavage sites. Green, blue, and yellow segments represent domains I, II, and III, respectively. The grey squares represent the five conserved amino acid blocks. Cleavage sites are indicated by arrows. The Ct end in the Toxin form depends on the processing agent (trypsin or gut fluid). Modified from Khorramnejad et al. [<a href="#B14-toxins-17-00067" class="html-bibr">14</a>].</p>
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<p>Production in <span class="html-italic">E. coli</span> cultures and purification of the Cry1Ia T-Int (<b>A</b>) and GF-Int (<b>B</b>) intermediary protein forms. (<b>A</b>) Lane 1: bacterial culture; Lane 2: supernatant from first culture centrifugation; Lane 3: sample after sonication; Lane 4: final supernatant; Lane 5: Blue Star molecular weight marker (Nippon Genetics Europe GmbH, Düren, Germany). Lanes 6–10 are samples from affinity chromatography purification, Lanes 6–8: eluted fractions 2, 3, and 4, respectively; Lane 9: flow-through; Lane 10: wash buffer flow-through. (<b>B</b>) Lane 1: Blue Star marker; Lanes 2 and 3: two independent bacterial cultures; Lane 4: pooled supernatant from centrifugation of both cultures; Lane 5: column flow-through; Lane 6: column wash; Lanes 7–10: eluted fractions 3, 4, 5, and 6, respectively. The numbers on the left indicate the molecular weight (in kDa) of the molecular weight marker bands. Double and triple arrows indicate the T-Int and GF-Int protein bands, respectively.</p>
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<p>Size exclusion chromatography analysis of Cry1Ia protein forms. (<b>A</b>) Protoxin. Lanes in the gel image are I: protein before SEC analysis; M: Blue Star marker (Nippon Genetics Europe GmbH, Düren, Germany); P1: chromatogram peak 1; B5 and B6: fractions between peaks 1 and 2; P2: peak 2; B8 and B9: fractions after peak 2. (<b>B</b>) T-Int sample. Lanes in the gel image are M: Blue Star marker; I: protein before SEC analysis; P2 and S2: chromatogram peak 2 and shoulder 2, respectively. (<b>C</b>) GF-Int samples. Lanes are M: Blue Star Marker; I: protein before SEC analysis; P1: peak 1; P1-P2: fractions in between peaks 1 and 2; P2: peak 2. (<b>D</b>) “Toxin” sample. Lanes in the gel image are I: protein before SEC analysis; M: Blue Star marker; P1: peak 1; P2: the two fractions of peak 2; P3: three fractions of peak 3; P4: peak 4; P5: peak 5. Numbers on the left of the gel images indicate the size of the molecular weight marker bands in kDa. Triangles and squares represent the dimeric and the monomeric form, respectively, of each protein.</p>
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<p>Processing of Cry1Ia protoxin (<b>A</b>), T-Int (<b>B</b>), and GF-Int (<b>C</b>) proteins with a 1:10 ratio of bovine trypsin to protein. Samples were resolved by SDS-PAGE and stained for total protein. Lanes 0′: Cry1Ia protein before trypsin addition; Lanes M: Blue Star marker (Nippon Genetics Europe GmbH, Düren, Germany); Lanes 1.5′ to 240′: time of processing with trypsin in minutes. The numbers on the left indicate the size of molecular weight marker bands in kDa. The protein bands of protoxin, T-Int, and GF-Int are indicated as simple, double, and triple arrows, respectively. The toxin bands are highlighted with an asterisk mark.</p>
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<p>Processing of Cry1Ia protoxin (<b>A</b>), T-Int (<b>B</b>), and GF-Int (<b>C</b>) proteins with a 1:10 ratio of gut fluids from <span class="html-italic">O. nubilalis</span> larvae to protein. Samples were resolved by SDS-PAGE and stained for total protein. Proteins in gut fluids without Cry1Ia proteins are shown in lanes GJ. Lanes M: Blue Star marker (Nippon Genetics Europe GmbH, Düren, Germany); Lanes 0′: protein before larvae gut fluid addition; Lanes 1.5′ to 180′: time of protein incubation with larval gut fluids in minutes. The numbers on the left indicate the size of molecular weight marker bands in kDa. The protein bands of protoxin, T-Int, and GF-Int are indicated as simple, double, and triple arrows, respectively. The toxin bands are highlighted with an asterisk mark.</p>
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<p>Toxicity of Cry1Ia protoxin (protoxin), fully processed toxin (toxin), and T-Int and GF-Int intermediate forms against <span class="html-italic">L. decemlineata</span> larvae. A discriminatory dose of 200 µg/mL was selected as producing 50–80% mortality in preliminary bioassays. Cry3Aa at 20 µg/mL was used as a positive control for mortality, and potato leaves coated with dilution buffer (tween-20) were used as a negative control for background mortality (&lt;10%). The data shown are the means and corresponding standard errors from a minimum of two bioassays, each with 45 larvae. Different letters on top of columns represent significant differences (Kruskal–Wallis One Way ANOVA on Ranks for not normally distributed data with Dunn’s Method for multiple comparisons, <span class="html-italic">p</span> &lt; 0.05).</p>
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21 pages, 12641 KiB  
Review
Using Klebsiella sp. and Pseudomonas sp. to Study the Mechanism of Improving Maize Seedling Growth Under Saline Stress
by Xiaoyu Zhao, Xiaofang Yu, Julin Gao, Jiawei Qu, Qinggeer Borjigin, Tiantian Meng and Dongbo Li
Plants 2025, 14(3), 436; https://doi.org/10.3390/plants14030436 - 2 Feb 2025
Viewed by 398
Abstract
The increasing salinization of cultivated soil worldwide has led to a significant reduction in maize production. Using saline–alkaline-tolerant growth-promoting bacteria (PGPR) in the rhizosphere can significantly improve the saline tolerance of maize and ensure the stability of maize yields, which has become a [...] Read more.
The increasing salinization of cultivated soil worldwide has led to a significant reduction in maize production. Using saline–alkaline-tolerant growth-promoting bacteria (PGPR) in the rhizosphere can significantly improve the saline tolerance of maize and ensure the stability of maize yields, which has become a global research hotspot. This study screened salt-tolerant microorganisms Klebsiella sp. (GF2) and Pseudomonas sp. (GF7) from saline soil to clarify the mechanism in improving the saline tolerance of maize. In this study, different application treatments (GF2, GF7, and GF2 + GF7) and no application (CK) were set up to explore the potential ecological relationships between the saline tolerance of maize seedlings, soil characteristics, and microorganisms. The results showed that co-occurrence network and Zi-Pi analysis identified Klebsiella and Pseudomonas as core microbial communities in the rhizosphere soil of maize seedlings grown in saline soil. The deterministic process of microbial assembly mainly controlled the bacterial community, whereas bacteria and fungi were governed by random processes. The application of saline–alkaline-resistant PGPR under saline stress significantly promoted maize seedling growth, increased the activity of soil growth-promoting enzymes, and enhanced total nitrogen, soil organic carbon, and microbial carbon and nitrogen contents. Additionally, it reduced soil salt and alkali ion concentrations [electrical conductivity (EC) and exchangeable Na+]. Among them, GF2 + GF7 treatment had the best effect, indicating that saline–alkaline-tolerant PGPR could directly or indirectly improve the saline tolerance of maize seedlings by improving the rhizosphere soil ecological environment. EC was the determining factor to promote maize seedling growth under saline–alkaline stress (5.56%; p < 0.01). The results provided an important theoretical reference that deciphers the role of soil factors and microecology in enhancing the saline tolerance of maize. Full article
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<p>Analysis of relevant indicators of growth-promoting functions of strains GF2, GF7, and GF2 + GF7. (<b>A</b>) EPS content, (<b>B</b>) IAA content, (<b>C</b>) iron chelator content (SDP), (<b>D</b>) ACC deaminase activity, (<b>E</b>) and phosphate solubilization (PSA). Lowercase letters represent significant differences at the <span class="html-italic">p</span> &lt; 0.05 level. The error bars represent standard deviation, and the circles indicate number of repetitions (n = 3).</p>
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<p>Growth and development of maize seedlings subjected to saline-tolerant PGPR at 40 days, and analysis of related indexes. (<b>A</b>) Maize HP, (<b>B</b>) SD, (<b>C</b>) CFE, (<b>D</b>) SPAD value, (<b>E</b>) FWP, (<b>F</b>) DWP, (<b>G</b>) MC, (<b>H</b>) LAI, (<b>I</b>) WR, and (<b>J</b>) observation on maize seedling growth under saline stress at 40 days. Lowercase letters represent significant differences at the <span class="html-italic">p</span> &lt; 0.05 level. The error bars represent standard deviation, and the circles indicate number of repetitions (n = 3).</p>
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<p>Analysis of growth-promoting enzyme activity in soil with PGPR. (<b>A</b>) SUE, (<b>B</b>) SSC, (<b>C</b>) ALP, (<b>D</b>) ALPT, (<b>E</b>) soil CAT, and (<b>F</b>) SCL. Lowercase letters represent significant differences at the <span class="html-italic">p</span> &lt; 0.05 level. The error bars represent standard deviation, and the circles indicate number of repetitions (n = 3).</p>
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<p>Analysis of microbial community structure diversity under different bacterial application treatments. PCoA and Bray−Curtis similarity index were used to analyze the microbial communities (ASV abundance) of bacteria (<b>A</b>) and fungi (<b>B</b>) in the soil of each treatment. The dominant phyla of bacteria (<b>C</b>) and fungi (<b>D</b>) among different treatments were analyzed by one-way variance comparison. The relative abundance of bacteria (<b>E</b>) and fungi (<b>F</b>) in the soil of different bacterial application treatments was analyzed by one-way variance comparison. All the data had three replicate values. * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>Ecological processes shaping soil bacterial and fungal communities under different bacterial application treatments by the zero model (<b>A</b>) and NCM (<b>B</b>,<b>C</b>). The horizontal axis is the log (mean relative abundance) of species, and the vertical axis is the predicted occurrence frequency. Points represent data values. The solid line represents the fit of the neutral model, and the upper and lower dashed lines represent the 95% confidence of the model prediction. <span class="html-italic">R</span><sup>2</sup> represents the overall goodness of fit of the neutral community model.</p>
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<p>Analysis of soil microbial co-occurrence network (<b>A</b>) and Zi-Pi module (<b>B</b>) in saline soil treated with bacterial application treatments. Based on the paired Pearson correlation between ASVs (<span class="html-italic">p</span> &gt; 0.8), nodes are colored by gate level and represent an operational taxon. The size of each node is proportional to the number of connections (degrees). The thickness of each connection between two nodes (edges) is proportional to the value of the Spearman correlation coefficient. Zi and Pi represent intramodule and intermodule connections, respectively. Network hub: nodes with Zi &gt; 2.5, Pi &gt; 0.62; module hub: Zi &gt; 2.5 and Pi ≤ 0.62; connector: Zi ≤ 2.5 and Pi &gt; 0.62; peripheral nodes: Zi ≤ 2.5 and Pi ≤ 0.62.</p>
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<p>Correlation analysis of soil bacteria and fungi under bacterial application treatments with the physical and chemical properties of maize seedlings and soil (<b>A</b>) and identification of key factors promoting the saline tolerance of maize seedlings through RF and Spearman correlation analysis (<b>B</b>). The potential direct and indirect effects of soil variables and bacterial and fungal diversity on the salinity tolerance of maize seedlings were analyzed based on PLS−PM (<b>C</b>). Soil properties are grouped into the same box in the model, and the numbers adjacent to the arrows indicate the magnitude of the influence of the relationship. * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001. Red indicates a positive correlation, and the blue line indicates a negative correlation. The width of the arrow is proportional to the strength of the path coefficient. Standardized direct effects, indirect effects, and total effects (<b>D</b>).</p>
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<p>Model analysis of soil traits, soil microbial diversity, and maize seedling growth in saline soil with the addition of PGPR. The red arrow represents the upward trend and the blue arrow represents the downward trend.</p>
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13 pages, 4564 KiB  
Article
Silica-Nanocoated Membranes with Enhanced Stability and Antifouling Performance for Oil-Water Emulsion Separation
by Mengfan Zhu, Chengqian Huang and Yu Mao
Membranes 2025, 15(2), 41; https://doi.org/10.3390/membranes15020041 - 1 Feb 2025
Viewed by 331
Abstract
Despite the potential of glass fiber (GF) membranes for oil-water emulsion separations, efficient surface modification methods to enhance fouling resistance while preserving membrane performance and stability remain lacking. We report a silica nanocoating method to modify GF membranes through a vapor deposition method. [...] Read more.
Despite the potential of glass fiber (GF) membranes for oil-water emulsion separations, efficient surface modification methods to enhance fouling resistance while preserving membrane performance and stability remain lacking. We report a silica nanocoating method to modify GF membranes through a vapor deposition method. The high smoothness (<1 nm r.m.s.) and high conformality of the vapor-deposited silica nanocoatings enabled the preservation of membrane microstructure and permeability, which, combined with the enhanced surface hydrophilicity, led to an oil rejection rate exceeding 99% and more than a 40% improvement in permeate flux in oil-water emulsion separations. Furthermore, the silica nanocoatings provided the membranes with excellent wet strength and stability against organic solvents, strong acids, oxidants, boiling, and sonication. The silica-nanocoated membrane demonstrated enhanced fouling resistance, achieving flux recovery higher than 75% during repeated oil-water emulsion separations and bovine serum albumin and humic acid fouling tests. Full article
(This article belongs to the Special Issue Membrane Separation and Water Treatment: Modeling and Application)
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<p>Preparation of silica-nanocoated GF membranes. The PTMSPMA was coated on GF membranes using iCVD, followed by in-air annealing at 400 °C for 1 h.</p>
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<p>FTIR spectra and water contact angle of as-deposited PTMSPMA and the silica nanocoatings formed after annealing.</p>
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<p>SEM images of (<b>a</b>) pristine GF and (<b>b</b>) GF/Si300. The insets show an enlarged view of the fibers. (<b>c</b>) Pure water permeability of the pristine, GF/Si100, and GF/Si300 membranes. Differences were considered statistically significant when * <span class="html-italic">p</span> &lt; 0.05.</p>
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<p>(<b>a</b>) Photos of membranes with 10 μL dyed water. The yellow, red, and purple lines indicate the stained (wetting) area of the top surface, intermediate layer, and bottom surface. (<b>b</b>) The underwater oil contact angle (UWOCA) of membranes.</p>
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<p>(<b>a</b>) Photos of the separation apparatus. Microscopy images of (<b>b</b>) SDS-stabilized diesel-in-water emulsion and the filtrates of (<b>c</b>) pristine GF, (<b>d</b>) GF/Si100, and (<b>e</b>) GF/Si300 membranes. Scale bar = 30 μm.</p>
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<p>Oil removal rates and permeate flux of pristine and silica-nanocoated membranes in emulsion separation. Differences were considered statistically significant when * <span class="html-italic">p</span> &lt; 0.05.</p>
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<p>(<b>a</b>) Decrease in oil rejection rates and (<b>b</b>) increase in membrane thickness of pristine and nanocoated membranes after the water boiling test. Optical microscopy images of the cross-section of the (<b>c</b>) pristine GF, (<b>d</b>) GF/Si100, and (<b>e</b>) GF/Si300 membranes after boiling for different periods.</p>
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<p>BSA adsorption on membranes after harsh treatments, including 24-h immersion in acetone (99.5%), H<sub>2</sub>SO<sub>4</sub> (96%), H<sub>2</sub>O<sub>2</sub> (30%), and NaOH (0.1 M), 1-h sonication in water, and 12-h immersion in boiling water.</p>
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<p>The anti-oil-fouling performance of pristine and silica-nanocoated GF membranes, as measured by normalized flux during the repeated filtrations of oil-in-water emulsions (SDS: 50 mg/L, diesel: 1% <span class="html-italic">v</span>/<span class="html-italic">v</span>).</p>
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<p>Normalized flux of the pristine and nanocoated membranes versus the cumulative permeate volume in the BSA fouling test.</p>
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<p>Normalized flux of the pristine and nanocoated membranes versus the cumulative permeate volume in the HA fouling test.</p>
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21 pages, 983 KiB  
Article
Discrete Cartesian Coordinate Transformations: Using Algebraic Extension Methods
by Aruzhan Kadyrzhan, Dinara Matrassulova, Yelizaveta Vitulyova and Ibragim Suleimenov
Appl. Sci. 2025, 15(3), 1464; https://doi.org/10.3390/app15031464 - 31 Jan 2025
Viewed by 493
Abstract
It is shown that it is reasonable to use Galois fields, including those obtained by algebraic extensions, to describe the position of a point in a discrete Cartesian coordinate system in many cases. This approach is applicable to any problem in which the [...] Read more.
It is shown that it is reasonable to use Galois fields, including those obtained by algebraic extensions, to describe the position of a point in a discrete Cartesian coordinate system in many cases. This approach is applicable to any problem in which the number of elements (e.g., pixels) into which the considered fragment of the plane is dissected is finite. In particular, it is obviously applicable to the processing of the vast majority of digital images actually encountered in practice. The representation of coordinates using Galois fields of the form GF(p2) is a discrete analog of the representation of coordinates in the plane through a complex variable. It is shown that two different types of algebraic extensions can be used simultaneously to represent transformations of discrete Cartesian coordinates described through Galois fields. One corresponds to the classical scheme, which uses irreducible algebraic equations. The second type proposed in this report involves the use of a formal additional solution of some equation, which has a usual solution. The correctness of this approach is justified through the representation of the elements obtained by the algebraic expansion of the second type by matrices defined over the basic Galois field. It is shown that the proposed approach is the basis for the development of new methods of information protection, designed to control groups of UAVs in the zone of direct radio visibility. The algebraic basis of such methods is the solution of systems of equations written in terms of finite algebraic structures. Full article
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<p>Illustration of the geometric problem corresponding to the determination of the operator’s coordinates. 1: UAVs forming a group. 2: Radio signal source. The branches of hyperbolas corresponding to two pairs of UAVs located at their focuses are represented by blue and red colors.</p>
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<p>Illustration to ensure the protection of information transmitted to a group of UAVs by solving the geometric problem. 1: UAV. 2: Communication channels. 3: Radio signal source (operator).</p>
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21 pages, 16401 KiB  
Article
High-Resolution Mapping of Maize in Mountainous Terrain Using Machine Learning and Multi-Source Remote Sensing Data
by Luying Liu, Jingyi Yang, Fang Yin and Linsen He
Land 2025, 14(2), 299; https://doi.org/10.3390/land14020299 - 31 Jan 2025
Viewed by 434
Abstract
In recent years, machine learning methods have garnered significant attention in the field of crop recognition, playing a crucial role in obtaining spatial distribution information and understanding dynamic changes in planting areas. However, research in smaller plots within mountainous regions remains relatively limited. [...] Read more.
In recent years, machine learning methods have garnered significant attention in the field of crop recognition, playing a crucial role in obtaining spatial distribution information and understanding dynamic changes in planting areas. However, research in smaller plots within mountainous regions remains relatively limited. This study focuses on Shangzhou District in Shangluo City, Shaanxi Province, utilizing a dataset of high-resolution remote sensing images (GF-1, ZY1-02D, ZY-3) collected over seven months in 2021 to calculate the normalized difference vegetation index (NDVI) and construct a time series. By integrating field survey results with time series images and Google Earth for visual interpretation, the NDVI time series curve for maize was analyzed. The Random Forest (RF) classification algorithm was employed for maize recognition, and comparative analyses of classification accuracy were conducted using Support Vector Machine (SVM), Gaussian Naive Bayes (GNB), and Artificial Neural Network (ANN). The results demonstrate that the random forest algorithm achieved the highest accuracy, with an overall accuracy of 94.88% and a Kappa coefficient of 0.94, both surpassing those of the other classification methods and yielding satisfactory overall results. This study confirms the feasibility of using time series high-resolution remote sensing images for precise crop extraction in the southern mountainous regions of China, providing valuable scientific support for optimizing land resource use and enhancing agricultural productivity. Full article
(This article belongs to the Section Land – Observation and Monitoring)
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<p>Location of the study area and land-use features: (<b>a</b>) administrative divisions of China; (<b>b</b>) administrative divisions of Shaanxi Province; (<b>c</b>) main natural rivers in Shangzhou District; (<b>d</b>) elevation map and distribution of maize planting points; (<b>e</b>) land-use status map of the study area in 2023.</p>
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<p>Maize phenological period and image acquisition dates.</p>
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<p>NDVI time series curves and the arithmetic mean spectral reflectance for different land cover types: (<b>a</b>) NDVI time series for major land cover types; (<b>b</b>) average spectral reflectance in the red band for major land cover types; (<b>c</b>) average spectral reflectance in the near-infrared band for major land cover types; the solid line is used to represent periods with continuous data, while the dashed line is used to connect periods with missing data.</p>
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<p>Annual variation trends of temperature, precipitation, and evapotranspiration in the study area from 2019 to 2023: (<b>a</b>) annual variation trend of temperature; (<b>b</b>) annual variation trend of precipitation; (<b>c</b>) annual variation trend of evapotranspiration.</p>
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<p>Distribution of accuracy for each machine learning method.</p>
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<p>Classification results of various machine learning methods: e, f, g, and h represent the four typical regions of the study area; (<b>a</b>) Gaussian Naive Bayes classification results; (<b>b</b>) Artificial Neural Network classification results; (<b>c</b>) Support Vector Machine classification results; (<b>d</b>) Random Forest classification results.</p>
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<p>Typical area classification results of various machine learning methods: (<b>a</b>–<b>d</b>) represent the classification results using Gaussian Naive Bayes for four typical regions of the study area; (<b>a1</b>,<b>b1</b>,<b>c1</b>,<b>d1</b>) represent the classification results using Artificial Neural Network; (<b>a2</b>,<b>b2</b>,<b>c2</b>,<b>d2</b>) represent the classification results using Support Vector Machine; (<b>a3</b>,<b>b3</b>,<b>c3</b>,<b>d3</b>) represent the classification results using Random Forest.</p>
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<p>Distribution of main crop maize in typical areas: (<b>a</b>–<b>d</b>) are the classification results of typical regions; (<b>a1</b>,<b>b1</b>,<b>c1</b>,<b>d1</b>) are the NDVI curves for maize in these regions; (<b>a2</b>,<b>b2</b>,<b>c2</b>,<b>d2</b>) are the images of these regions; (<b>a3</b>,<b>b3</b>,<b>c3</b>,<b>d3</b>) are field photographs of these regions.</p>
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14 pages, 3059 KiB  
Article
High Sensitivity and Wide Strain Range Flexible Strain Sensor Based on CB/CNT/PDA/TPU Conductive Fiber Membrane
by Qiong Wei, Zihang Sun, Xudong Li, Zichao Chen and Yi Li
Appl. Sci. 2025, 15(3), 1461; https://doi.org/10.3390/app15031461 - 31 Jan 2025
Viewed by 432
Abstract
Flexible strain sensors have attracted significant attention due to their critical applications in wearable devices, biological detection, and artificial intelligence. However, achieving both a wide strain range and high sensitivity remains a major challenge in current research. This study aims to develop a [...] Read more.
Flexible strain sensors have attracted significant attention due to their critical applications in wearable devices, biological detection, and artificial intelligence. However, achieving both a wide strain range and high sensitivity remains a major challenge in current research. This study aims to develop a novel composite material with a synergistic conductive network to construct high-performance flexible strain sensors. Thermoplastic polyurethane (TPU) nanofiber membranes were first prepared using electrospinning technology, and their surface was modified with polydopamine (PDA) via in-situ polymerization, which significantly enhanced the fibers’ adsorption capacity for conductive materials. Subsequently, carbon nanotubes (CNTs) and carbon black (CB) were coated onto the PDA-modified TPU fibers through ultrasonic anchoring, forming a CB/CNT/PDA/TPU composite with a synergistic conductive network. The results demonstrated that the flexible strain sensor fabricated from this composite material (with a CB-to-CNT mass ratio of 7:3) achieved ultrahigh sensitivity (gauge factor, GF, up to 1063) over a wide strain range (up to 300%), along with a low detection limit (1% strain), fast response and recovery times (137 ms), and exceptional stability and durability. Further evaluations confirmed that this sensor reliably captured biological signals from various joint movements, highlighting its broad application potential in human motion monitoring, human–machine interaction, and soft robotics. Full article
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<p>Schematic diagram of the fabrication process for the CB/CNT/PDA/TPU strain sensor.</p>
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<p>SEM images of fiber membranes: (<b>a</b>–<b>a”</b>) TPU, (<b>b</b>–<b>b”</b>) PDA/TPU, (<b>c</b>–<b>c”</b>) CB/CNT/TPU, and (<b>d</b>–<b>d”</b>) CB/CNT/PDA/TPU.</p>
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<p>(<b>a</b>) TG curves and (<b>b</b>) DTG curves of CB/CNT/TPU and CB/CNT/PDA/TPU; (<b>c</b>) FTIR spectra of TPU, PDA/TPU, and CB/CNT/PDA/TPU; (<b>d</b>) XRD patterns of TPU, PDA/TPU, and CB/CNT/PDA/TPU.</p>
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<p>(<b>a</b>–<b>c</b>) Thickness and images of CB/CNT/PDA/TPU under twisting and bending. The thickness of the sensor is 0.22 mm; (<b>d</b>,<b>e</b>) Stress–strain curves, and bar charts of tensile strength and elongation at break for TPU, PDA/TPU, CB/CNT/TPU, and CB/CNT/PDA/TPU.</p>
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<p>(<b>a</b>) Relative resistance change in mixed conductive fillers with different mass ratios during tensile process; (<b>b</b>) Relative resistance change in CB/CNT 7:3; (<b>c</b>) Response and recovery curves at 1% strain; (<b>d</b>) Stepwise cyclic response of CB/CNT 7:3; (<b>e</b>) Comparison of GF and working range of the sensor prepared in this work with those in the literature [<a href="#B18-applsci-15-01461" class="html-bibr">18</a>,<a href="#B19-applsci-15-01461" class="html-bibr">19</a>,<a href="#B20-applsci-15-01461" class="html-bibr">20</a>,<a href="#B21-applsci-15-01461" class="html-bibr">21</a>,<a href="#B22-applsci-15-01461" class="html-bibr">22</a>,<a href="#B23-applsci-15-01461" class="html-bibr">23</a>,<a href="#B24-applsci-15-01461" class="html-bibr">24</a>,<a href="#B25-applsci-15-01461" class="html-bibr">25</a>,<a href="#B26-applsci-15-01461" class="html-bibr">26</a>,<a href="#B27-applsci-15-01461" class="html-bibr">27</a>].</p>
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<p>(<b>a</b>) Relative resistance changes in the CB/CNT/PDA/TPU flexible strain sensor under low strain ranges (1%, 5%, 10%, 15%, 20%); (<b>b</b>) Relative resistance changes under high strain ranges (50%, 70%, 100%, 120%, 150%); (<b>c</b>) Long-term durability of the CB/CNT/PDA/TPU flexible strain sensor under 70% strain, with an enlarged view of the local signal. The purple color box represents 700–1000 ms, and the orange color box represents 4700–5000 ms.</p>
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<p>Applications of the CCPT flexible strain sensor in monitoring various human motions: (<b>a</b>,<b>b</b>) Sensor response to finger bending and releasing; (<b>c</b>,<b>d</b>) Sensor response to different elbow bending angles; (<b>e</b>) Sensor response to continuous wrist flexion movements; (<b>f</b>) Sensor response to continuous knee bending and straightening motions.</p>
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20 pages, 28282 KiB  
Article
Nanotechnological Formulation Incorporating Pectis brevipedunculata (Asteraceae) Essential Oil: An Ecofriendly Approach for Leishmanicidal and Anti-Inflammatory Therapy
by Estela Mesquita Marques, Lucas George Santos Andrade, Luciana Magalhães Rebelo Alencar, Erick Rafael Dias Rates, Rachel Melo Ribeiro, Rafael Cardoso Carvalho, Glécilla Colombelli de Souza Nunes, Daniele Stéfanie Sara Lopes Lera-Nonose, Maria Julia Schiavon Gonçalves, Maria Valdrinez Campana Lonardoni, Melissa Pires Souza, Emmanoel Vilaça Costa and Renato Sonchini Gonçalves
Polymers 2025, 17(3), 379; https://doi.org/10.3390/polym17030379 - 30 Jan 2025
Viewed by 459
Abstract
Cutaneous leishmaniasis caused by Leishmania amazonensis is a significant public health issue. This study aimed to evaluate an ecofriendly, thermosensitive nanogel, developed using a low-energy, solvent-free method, incorporating F127 and Carbopol 974P copolymers, and enriched with Pectis brevipedunculata essential oil (EOPb) [...] Read more.
Cutaneous leishmaniasis caused by Leishmania amazonensis is a significant public health issue. This study aimed to evaluate an ecofriendly, thermosensitive nanogel, developed using a low-energy, solvent-free method, incorporating F127 and Carbopol 974P copolymers, and enriched with Pectis brevipedunculata essential oil (EOPb) for its leishmanicidal and anti-inflammatory properties. The nanogel was prepared and characterized through FTIR, DLS, SEM, and AFM to confirm the incorporation of EOPb as well as its stability and rheological properties. In vitro leishmanicidal activity was evaluated on Leishmania amazonensis promastigotes, and in vivo anti-inflammatory effects were assessed using a rat paw edema model. In vitro, nGF3 (EOPb-loaded nanogel) demonstrated significant leishmanicidal activity, with promastigote mortality rates exceeding 80% at 24 h and 90% at 48 h. In vivo, nGF1, nGF2, and nGF3 exhibited anti-inflammatory effects, with nGF2 and nGF3 reducing edema by 62.7% at 2 h post-treatment. The empty nanogel (nGF0) showed minimal anti-inflammatory activity. The ecofriendly EOPb-loaded nanogel (nGF3) demonstrated strong leishmanicidal and anti-inflammatory effects, presenting a promising candidate for cutaneous leishmaniasis treatment. Further studies are necessary to explore its clinical potential. Full article
(This article belongs to the Special Issue Functional Gel and Their Multipurpose Applications)
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<p>Experimental sequence for the OE<span class="html-italic">Pb</span> extraction procedure: Collection of <span class="html-italic">Pb</span> (<b>A</b>), air drying of the plant material (<b>B</b>), grinding and hydrodistillation under controlled conditions (<b>C</b>), followed by drying and storage of OE<span class="html-italic">Pb</span> in a sealed amber vial (<b>D</b>). The nanogel preparation methodology was performed using a low-energy, solvent-free procedure (<b>E</b>). Photograph of the nanogels nGF1–nGF3 (<b>F</b>), along with a schematic representation of the structural organization of the F127/974P polymer blend and OE<span class="html-italic">Pb</span>-loading F127 micelles (<b>G</b>). Chemical structure of the polymers used in the preparation of the nanogels and chemical composition of the major chemical constituents of EO<span class="html-italic">Pb</span> (<b>H</b>).</p>
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<p>AFM force curves. (<b>A</b>) Topographic map of the sample, displaying distinct contrasts for higher regions (brighter areas) and lower regions (darker areas). Point 1 (Pt 1) corresponds to the specific region where the force curve was acquired. (<b>B</b>) Representative force curve obtained from Point 1 in image A, illustrating the approach (blue) and retraction (red) cycles. The indices along the curve highlight the distinct stages of the probe–sample interaction, as detailed in the graphical insets: (1) the probe is distant from the sample surface, (2) the probe establishes contact with the sample surface, (3) the linear regime of the curve during indentation, (4) the maximum resistance observed during probe–sample separation, (5) the probe retracts from the sample, and (6) the approach–retraction cycle concludes.</p>
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<p>Experimental sequence for in vivo evaluation of the anti-inflammatory potential of nanogels.</p>
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<p>FTIR spectra: (<b>A</b>) Essential oil of <span class="html-italic">Pectis brevipedunculata</span> (EO<span class="html-italic">Pb</span>) and (<b>B</b>) nanogel formulation containing 1% <span class="html-italic">w</span>/<span class="html-italic">w</span> EO<span class="html-italic">Pb</span> (nGF3).</p>
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<p>SEM micrographs of nGF0 at a magnification of 1000× (<b>A</b>), 2000× (<b>B</b>,<b>C</b>), and 5000× (<b>D</b>) after the freeze-drying process.</p>
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<p>SEM micrographs of nGF3 at a magnification of 500× (<b>A</b>), 1000× (<b>B</b>), 2000× (<b>C</b>), and 5000× (<b>D</b>) after the freeze-drying process. The SEM micrographs show well-defined planar regions with the absence of pores.</p>
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<p>SEM micrographs of nGF3 at a magnification of 1000× (<b>A</b>), 2000× (<b>B</b>,<b>C</b>), and 5000× (<b>D</b>) after the freeze-dying process. The SEM micrographs show thick planes organized in layers.</p>
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<p>AFM topographic maps of the nGF0 nanogel. (<b>A</b>) Yellow arrows indicate spherical structures formed by F127 micelles, with an average height of 108.74 ± 19.41 nm (n = 19), and green arrows indicate flat regions with an average height of 5.92 ± 3.00 nm (n = 15). (<b>B</b>,<b>D</b>) Expanded 3D micrographs showing the spherical structures in detail. (<b>C</b>) Cubic micellar structures formed by aggregational processes of F127 micelles.</p>
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<p>Young’s modulus maps (<b>A</b>,<b>B</b>) and adhesion force maps (<b>C</b>,<b>D</b>) were acquired for the different domains observed in the nGF0.</p>
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<p>Topographical AFM maps of the nanogel nGF3. (<b>A</b>) Red arrows indicate flat structures formed by the presence of EO<span class="html-italic">Pb</span> on the surface of the nGF0 matrix, with an average height of 7.39 ± 0.79 nm (n = 17). (<b>B</b>) Green arrows indicate the incorporation of EO<span class="html-italic">Pb</span> into the F127 micellar structures and the pores of the nGF0 material, with average height values of 1.37 ± 0.21 nm (n = 20) and size of 40.58 ± 7.98 nm (n = 20). (<b>C</b>,<b>D</b>) 3D micrographs showing the flat nGF3 structures.</p>
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<p>Maps of Young’s modulus (<b>A</b>,<b>B</b>) and adhesion forces (<b>C</b>,<b>D</b>) acquired for the nanogel nGF3.</p>
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<p>(<b>A</b>) In vitro leishmanicidal activity of nanogel formulation (nGF3) demonstrating concentration- and time-dependent efficacy against <span class="html-italic">LLa</span> (* <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, Kruskal–Wallis with Dunn’s post hoc test). (<b>B</b>) Representative images of <span class="html-italic">Leishmania</span> cultures treated with the nanogel formulation (nGF3): (a) Untreated culture after 24 h. (b) Culture treated with the nanogel (nGF0) for 24 h. (c–h) Cultures treated with OE<span class="html-italic">Pb</span> at concentrations of 2.2, 1.1, 0.55, 0.28, 0.14, and 0.07 mg/mL, respectively, for 24 h. (i) Untreated culture after 48 h. (j) Culture treated with nGF0 for 48 h. (k–p) Cultures treated with OE<span class="html-italic">Pb</span> at concentrations of 2.2, 1.1, 0.55, 0.28, 0.14, and 0.07 mg/mL, respectively, for 48 h.</p>
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<p>Topical anti-inflammatory action of nanogels in an experimental paw edema model in mice (* <span class="html-italic">p</span> &lt; 0.05, ANOVA, Tukey’s test).</p>
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