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Search Results (8,593)

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15 pages, 941 KiB  
Article
Embedding Tree-Based Intrusion Detection System in Smart Thermostats for Enhanced IoT Security
by Abbas Javed, Muhammad Naeem Awais, Ayyaz-ul-Haq Qureshi, Muhammad Jawad, Jehangir Arshad and Hadi Larijani
Sensors 2024, 24(22), 7320; https://doi.org/10.3390/s24227320 (registering DOI) - 16 Nov 2024
Abstract
IoT devices with limited resources, and in the absence of gateways, become vulnerable to various attacks, such as denial of service (DoS) and man-in-the-middle (MITM) attacks. Intrusion detection systems (IDS) are designed to detect and respond to these threats in IoT environments. While [...] Read more.
IoT devices with limited resources, and in the absence of gateways, become vulnerable to various attacks, such as denial of service (DoS) and man-in-the-middle (MITM) attacks. Intrusion detection systems (IDS) are designed to detect and respond to these threats in IoT environments. While machine learning-based IDS have typically been deployed at the edge (gateways) or in the cloud, in the absence of gateways, the IDS must be embedded within the sensor nodes themselves. Available datasets mainly contain features extracted from network traffic at the edge (e.g., Raspberry Pi/computer) or cloud servers. We developed a unique dataset, named as Intrusion Detection in the Smart Homes (IDSH) dataset, which is based on features retrievable from microcontroller-based IoT devices. In this work, a Tree-based IDS is embedded into a smart thermostat for real-time intrusion detection. The results demonstrated that the IDS achieved an accuracy of 98.71% for binary classification with an inference time of 276 microseconds, and an accuracy of 97.51% for multi-classification with an inference time of 273 microseconds. Real-time testing showed that the smart thermostat is capable of detecting DoS and MITM attacks without relying on a gateway or cloud. Full article
(This article belongs to the Special Issue Sensor Data Privacy and Intrusion Detection for IoT Networks)
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<p>Proposed architecture of embedded IDS for smart thermostats.</p>
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<p>Dataset collection on smart thermostats.</p>
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<p>Comparison of IDS implemented with quantization and without quantization.</p>
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<p>Comparison of IDS implemented with CatBoost and XGBoost on the smart thermostat.</p>
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21 pages, 6767 KiB  
Article
Dynamics of Arbuscular Mycorrhizal Fungi in the Rhizosphere of Medicinal Plants and Their Promotion on the Performance of Astragalus mongholicus
by Wanyi Zhang, Chao He, Yuli Lin, Shenghui Qin, Duo Wang, Chunmiao Li, Min Li, Xiang Sun and Xueli He
Agronomy 2024, 14(11), 2695; https://doi.org/10.3390/agronomy14112695 (registering DOI) - 15 Nov 2024
Abstract
Arbuscular mycorrhizal fungi (AMF) act as intermediaries between the root systems of host plants and the surrounding soil, offering various benefits to medicinal plants, such as promoting growth and enhancing quality. However, the host range of AMF in medicinal plants and the characteristics [...] Read more.
Arbuscular mycorrhizal fungi (AMF) act as intermediaries between the root systems of host plants and the surrounding soil, offering various benefits to medicinal plants, such as promoting growth and enhancing quality. However, the host range of AMF in medicinal plants and the characteristics of plant–AMF networks in farmland ecosystems remain insufficiently studied. In the present study, we measured AMF colonization, species diversity, and soil properties of 31 medicinal plants at the Anguo Medicine Planting Base in Northwest China. The medicinal plant–AMF network was subsequently analyzed, and the growth-promoting effects of AMF on Astragalus mongholicus were examined. Spore density, species richness, and total colonization exhibited significant variation across different medicinal plant species. Glomus melanosporum, G. claroideum, and Septoglomus constrictum were the dominant species among 61 AMF species. Soil organic matter, phosphatase, available nitrogen, and glomalin-related soil proteins (GRSPs) were the main factors affecting the AMF composition. Structural equation models and a variation partitioning analysis suggested a highly plant species-specific pattern of AMF distribution patterns, where the host identities explained 61.4% of changes in spore density and 48.2% of AMF colonization. The soil nutrient availability and phosphatase activity also influenced AMF colonization. Our results confirmed glomalin as an important contributor to the soil carbon in farmland for cultivating medicinal plants. The medicinal plant–AMF symbiotic network exhibited highly nested patterns, a low specialized structure, high connectance, and low modularity, which suggested saturated AMF colonization and symbiosis stability provided by redundant plant–AMF associations. Despite the wide host range among medicinal plants, AMF inoculation revealed species-specific effects on the growth performance and active ingredient content levels in A. mongholicus, G. claroideum and Sep. constrictum induced the highest biomass and active ingredient content accumulation in A. mongholicus. These findings advance our understanding of AMF community dynamics in the rhizosphere of medicinal plants and offer valuable insights for optimizing medicinal plant cultivation practices. Full article
(This article belongs to the Section Horticultural and Floricultural Crops)
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<p>Arbuscular mycorrhizal fungal (AMF) colonization in roots of medicinal plants. H = AMF hyphae; V = AMF vesicular. (<b>a</b>) <span class="html-italic">Aristolochia contorta</span>, (<b>b</b>) <span class="html-italic">Atractylodes macrocephala</span>, (<b>c</b>) <span class="html-italic">Sedum sarmentosum</span>, (<b>d</b>) <span class="html-italic">Scutellaria barbata</span>, (<b>e</b>) <span class="html-italic">Mimosa pudica</span>, (<b>f</b>) <span class="html-italic">Hosta plantaginea</span>, (<b>g</b>) <span class="html-italic">Lilium davidii</span>, (<b>h</b>) <span class="html-italic">Linum perenne</span>, (<b>i</b>) <span class="html-italic">Paeonia suffruticosa</span>, (<b>j</b>) <span class="html-italic">Stemona japonica</span>, (<b>k</b>) <span class="html-italic">Rehmannia glutinosa</span>, (<b>l</b>) <span class="html-italic">Angelica dahurica</span>.</p>
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<p>Arbuscular mycorrhizal fungal (AMF) total colonization rate (%) in medicinal plant roots. Different letters above the error bars indicate significant differences.</p>
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<p>Relative abundance (%) of arbuscular mycorrhizal fungal (AMF) genus (<b>a</b>) and species (<b>b</b>) level in rhizosphere soil of medicinal plants.</p>
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<p>Differences in arbuscular mycorrhizal fungal (AMF) spore richness ((<b>a</b>), number of species) and the Simpson index (<b>b</b>) at the family level of medicinal plants. Different letters indicate significant differences.</p>
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<p>The relative contributions of plant species and soil factors were evaluated using a variation partitioning analysis on arbuscular mycorrhizal fungal (AMF) total colonization (<b>a</b>) and spore density (<b>b</b>). Values &lt; 0 are not shown.</p>
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<p>Structural equation model illustrating the causal connections between plant species, arbuscular mycorrhizal fungal (AMF) communities, and soil parameters. The final model fitted the data well, with the maximum likelihood, x<sup>2</sup> = 35.667, df = 13, <span class="html-italic">p</span> = 0.01, RMSEA = 0.138, GFI = 0.918, AIC = 81.667, and CFI = 0.905. Solid lines represent significant pathways, while dashed lines denote nonsignificant ones. The thickness of the solid lines corresponds to the strength of the causal effect, and the numbers adjacent to the arrows show the standardized path coefficients. “e” represents the residual values. TG: total extractable glomalin-related soil protein.</p>
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<p>The bipartite interaction network formed by medicinal plants (lower boxes) and arbuscular mycorrhizal fungal (AMF) spores. The colors of the different lower boxes represent different medicinal plants.</p>
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<p>The impacts of arbuscular mycorrhizal fungi (AMF) on the growth parameters of <span class="html-italic">Astragalus mongholicus</span> seedlings. Growth diagram (<b>a</b>), plant height (<b>b</b>), branch number (<b>c</b>), plant biomass (<b>d</b>), blade number (<b>e</b>), root length (<b>f</b>), Calycosin-7-glucoside (<b>g</b>), and formononetin (<b>h</b>). Different letters above the error bars indicate significant differences. CK, inoculated control; GM, <span class="html-italic">Glomus melanosporum</span>; GC, <span class="html-italic">Glmous. claroideum</span>; SC, <span class="html-italic">Septoglomus constrictum</span>.</p>
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13 pages, 2412 KiB  
Article
Preparation and Study of Poly(Vinylidene Fluoride-Co-Hexafluoropropylene)-Based Composite Solid Electrolytes
by Meihong Huang, Lingxiao Lan, Pengcheng Shen, Zhiyong Liang, Feng Wang, Yuling Zhong, Chaoqun Wu, Fanxiao Kong and Qicheng Hu
Crystals 2024, 14(11), 982; https://doi.org/10.3390/cryst14110982 - 14 Nov 2024
Viewed by 168
Abstract
Solid-state electrolytes are widely anticipated to revitalize lithium-ion batteries with high energy density and safety. However, low ionic conductivity and high interfacial resistance at room temperature pose challenges for practical applications. This study combines the rigid oxide electrolyte LLZTO with the flexible polymer [...] Read more.
Solid-state electrolytes are widely anticipated to revitalize lithium-ion batteries with high energy density and safety. However, low ionic conductivity and high interfacial resistance at room temperature pose challenges for practical applications. This study combines the rigid oxide electrolyte LLZTO with the flexible polymer electrolyte poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) to achieve effective coupling of rigidity and flexibility. The semi-interpenetrating network structure endows the PEL composite solid electrolyte with excellent lithium-ion transport capabilities, resulting in an ionic conductivity of up to 5.1 × 10−4 S cm−1 and lithium-ion transference number of 0.41. The assembled LiFePO4/PEL/Li solid-state battery demonstrates an initial discharge capacity of 132 mAh g−1 at a rate of 0.1 C. After 100 charge–discharge cycles, the capacity retention is 81%. This research provides a promising strategy for preparing composite solid electrolytes in solid-state lithium-ion batteries. Full article
(This article belongs to the Special Issue Research on Electrolytes and Energy Storage Materials)
22 pages, 2678 KiB  
Review
A Comprehensive Review of Optimizing Multi-Energy Multi-Objective Distribution Systems with Electric Vehicle Charging Stations
by Mahesh Kumar, Aneel Kumar, Amir Mahmood Soomro, Mazhar Baloch, Sohaib Tahir Chaudhary and Muzamil Ahmed Shaikh
World Electr. Veh. J. 2024, 15(11), 523; https://doi.org/10.3390/wevj15110523 - 14 Nov 2024
Viewed by 265
Abstract
Electric vehicles worldwide provide numerous key advantages in the energy sector. They are advantageous over fossil fuel vehicles in many aspects: for example, they consume no fuel, are economical, and only require charging the internal batteries, which power the motor for propulsion. Thus, [...] Read more.
Electric vehicles worldwide provide numerous key advantages in the energy sector. They are advantageous over fossil fuel vehicles in many aspects: for example, they consume no fuel, are economical, and only require charging the internal batteries, which power the motor for propulsion. Thus, due to their numerous advantages, research is necessary to improve the technological aspects that can enhance electric vehicles’ overall performance and efficiency. However, electric vehicle charging stations are the key hindrance to their adoption. Charging stations will affect grid stability and may lead to altering different parameters, e.g., power losses and voltage deviation when integrated randomly into the distribution system. The distributed generation, along with charging stations with the best location and size, can be a solution that mitigates the above concerns. Metaheuristic techniques can be used to find the optimal siting and sizing of distributed generations and electric vehicle charging stations. This review provides an exhaustive review of various methods and scientific research previously undertaken to optimize the placement and dimensions of electric vehicle charging stations and distributed generation. We summarize the previous work undertaken over the last five years on the multi-objective placement of distributed generations and electric vehicle charging stations. Key areas have focused on optimization techniques, technical parameters, IEEE networks, simulation tools, distributed generation types, and objective functions. Future development trends and current research have been extensively explored, along with potential future advancement and gaps in knowledge. Therefore, at the conclusion of this review, the optimization of electric vehicle charging stations and distributed generation presents both the practical and theoretical importance of implementing metaheuristic algorithms in real-world scenarios. In the same way, their practical integration will provide the transportation system with a robust and sustainable solution. Full article
(This article belongs to the Special Issue Fast-Charging Station for Electric Vehicles: Challenges and Issues)
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<p>Optimization techniques.</p>
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<p>Objective functions.</p>
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<p>Networks used previously.</p>
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<p>Energy sources used in the literature.</p>
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<p>Electric vehicle charging infrastructure [<a href="#B68-wevj-15-00523" class="html-bibr">68</a>].</p>
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<p>Electric vehicle levels, methods, and modes [<a href="#B67-wevj-15-00523" class="html-bibr">67</a>].</p>
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<p>Electric vehicle batteries [<a href="#B67-wevj-15-00523" class="html-bibr">67</a>].</p>
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<p>Converters in electric vehicles.</p>
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<p>Categorization of the optimization methods used for concurrent DG-EVCS-SCB allocation.</p>
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26 pages, 4934 KiB  
Article
Capacity and Coverage Dimensioning for 5G Standalone Mixed-Cell Architecture: An Impact of Using Existing 4G Infrastructure
by Naba Raj Khatiwoda, Babu Ram Dawadi and Sashidhar Ram Joshi
Future Internet 2024, 16(11), 423; https://doi.org/10.3390/fi16110423 - 14 Nov 2024
Viewed by 308
Abstract
With the increasing demand for expected data volume daily, current telecommunications infrastructure can not meet requirements without using enhanced technologies adopted by 5G and beyond networks. Due to their diverse features, 5G technologies and services will be phenomenal in the coming days. Proper [...] Read more.
With the increasing demand for expected data volume daily, current telecommunications infrastructure can not meet requirements without using enhanced technologies adopted by 5G and beyond networks. Due to their diverse features, 5G technologies and services will be phenomenal in the coming days. Proper planning procedures are to be adopted to provide cost-effective and quality telecommunication services. In this paper, we planned 5G network deployment in two frequency ranges, 3.5 GHz and 28 GHz, using a mixed cell structure. We used metaheuristic approaches such as Grey Wolf Optimization (GWO), Sparrow Search Algorithm (SSA), Whale Optimization Algorithm (WOA), Marine Predator Algorithm (MPA), Particle Swarm Optimization (PSO), and Ant Lion Optimization (ALO) for optimizing the locations of remote radio units. The comparative analysis of metaheuristic algorithms shows that the proposed network is efficient in providing an average data rate of 50 Mbps, can meet the coverage requirements of at least 98%, and meets quality-of-service requirements. We carried out the case study for an urban area and another suburban area of Kathmandu Valley, Nepal. We analyzed the outcomes of 5G greenfield deployment and 5G deployment using existing 4G infrastructure. Deploying 5G networks using existing 4G infrastructure, resources can be saved up to 33.7% and 54.2% in urban and suburban areas, respectively. Full article
(This article belongs to the Topic Advances in Wireless and Mobile Networking)
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<p>5G- mixed cell structure.</p>
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<p>Proposed 5G network optimization framework.</p>
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<p>Case I: Urban 5G greenfield.</p>
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<p>Case II: Urban 5G with existing 4G.</p>
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<p>Case I: Suburban 5G greenfield.</p>
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<p>Case II: Suburban 5G with existing 4G.</p>
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<p>Convergence urban 5G.</p>
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<p>Execution time.</p>
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<p>Coverage urban 5G.</p>
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<p>Best optimized Urban 5G.</p>
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<p>Convergence.</p>
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<p>Execution time.</p>
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<p>MPA.</p>
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<p>ALO.</p>
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<p>Coverage percentage.</p>
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<p>Best optimized location urban 5G.</p>
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<p>Coverage urban macro-RRUs.</p>
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<p>Coverage cell macro-RRUs.</p>
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<p>Mixed cell 5G greenfield.</p>
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<p>Mixed cell in the field.</p>
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<p>Mixed cell with existing 4G sites.</p>
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<p>Mixed cell in the field.</p>
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<p>Convergence suburban.</p>
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<p>Suburban Coverage.</p>
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<p>Final optimized deployment.</p>
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<p>Best-optimized RRUs in the field.</p>
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<p>Convergence suburban.</p>
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<p>Suburban Coverage.</p>
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<p>Final optimized deployment.</p>
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<p>Field implementation.</p>
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19 pages, 5737 KiB  
Article
Improving the Quality of Experience of Video Streaming Through a Buffer-Based Adaptive Bitrate Algorithm and Gated Recurrent Unit-Based Network Bandwidth Prediction
by Jeonghun Woo, Seungwoo Hong, Donghyun Kang and Donghyeok An
Appl. Sci. 2024, 14(22), 10490; https://doi.org/10.3390/app142210490 - 14 Nov 2024
Viewed by 289
Abstract
With the evolution of cellular networks and wireless-local-area-network-based communication technologies, services for smart device users have appeared. With the popularity of 4G and 5G, smart device users can now consume larger bandwidths than before. Consequently, the demand for various services, such as streaming, [...] Read more.
With the evolution of cellular networks and wireless-local-area-network-based communication technologies, services for smart device users have appeared. With the popularity of 4G and 5G, smart device users can now consume larger bandwidths than before. Consequently, the demand for various services, such as streaming, online games, and video conferences, has increased. For improved quality of experience (QoE), streaming services utilize adaptive bitrate (ABR) algorithms to handle network bandwidth variations. ABR algorithms use network bandwidth history for future network bandwidth prediction, allowing them to perform efficiently when network bandwidth fluctuations are minor. However, in environments with frequent network bandwidth changes, such as wireless networks, the QoE of video streaming often degrades because of inaccurate predictions of future network bandwidth. To address this issue, we utilize the gated recurrent unit, a time series prediction model, to predict the network bandwidth accurately. We then propose a buffer-based ABR streaming technique that selects optimized video-quality settings on the basis of the predicted bandwidth. The proposed algorithm was evaluated on a dataset provided by Zeondo by categorizing instances of user mobility into walking, bus, and train scenarios. The proposed algorithm improved the QoE by approximately 11% compared with the existing buffer-based ABR algorithm in various environments. Full article
(This article belongs to the Special Issue Multimedia Systems Studies)
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<p>Total volume of app categories in 2022.</p>
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<p>Buffer occupancy calculation.</p>
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<p>Video quality selection in the buffer-based adaptive bitrate algorithm.</p>
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<p>Bandwidth measurement results.</p>
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<p>Ratio of measurements recorded as zero among total measurements.</p>
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<p>Normalized root meant squared error (NRMSE) values with different hyperparameter settings.</p>
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<p>GRU model structure.</p>
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<p>Structure of the proposed scheme.</p>
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<p>Relationship between video rate and buffer occupancy.</p>
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<p>Root mean square error (RMSE) of network bandwidth prediction in different mobility scenarios.</p>
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<p>Comparison of the measured and predicted network bandwidths in the pedestrian scenario.</p>
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<p>Comparison of the measured and predicted network bandwidths in the bus scenario.</p>
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<p>Comparison of the measured and predicted network bandwidths in train scenario.</p>
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<p>Comparison of mean opinion scores in pedestrian scenarios.</p>
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<p>Comparison of video quality in pedestrian scenario 2.</p>
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<p>Comparison of buffer occupancies in pedestrian scenario 2.</p>
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<p>Comparison of mean opinion scores in bus scenarios.</p>
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<p>Comparison of video quality in bus scenario 7.</p>
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<p>Comparison of buffer occupancy in bus scenario 7.</p>
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<p>Comparison of the mean opinion scores in train scenarios.</p>
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<p>Comparison of video quality in train scenario 1.</p>
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<p>Comparison of buffer occupancy in train scenario 1.</p>
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17 pages, 4282 KiB  
Article
A Recognition Model Based on Multiscale Feature Fusion for Needle-Shaped Bidens L. Seeds
by Zizhao Zhang, Yiqi Huang, Ying Chen, Ze Liu, Bo Liu, Conghui Liu, Cong Huang, Wanqiang Qian, Shuo Zhang and Xi Qiao
Agronomy 2024, 14(11), 2675; https://doi.org/10.3390/agronomy14112675 - 14 Nov 2024
Viewed by 223
Abstract
To solve the problem that traditional seed recognition methods are not completely suitable for needle-shaped seeds, such as Bidens L., in agricultural production, this paper proposes a model construction idea that combines the advantages of deep residual models in extracting high-level abstract features [...] Read more.
To solve the problem that traditional seed recognition methods are not completely suitable for needle-shaped seeds, such as Bidens L., in agricultural production, this paper proposes a model construction idea that combines the advantages of deep residual models in extracting high-level abstract features with multiscale feature extraction fusion, taking into account the depth and width of the network. Based on this, a multiscale feature fusion deep residual network (MSFF-ResNet) is proposed, and image segmentation is performed before classification. The image segmentation is performed by a popular semantic segmentation method, U2Net, which accurately separates seeds from the background. The multiscale feature fusion network is a deep residual model based on a residual network of 34 layers (ResNet34), and it contains a multiscale feature fusion module and an attention mechanism. The multiscale feature fusion module is designed to extract features of different scales of needle-shaped seeds, while the attention mechanism is used to improve the ability to select features of our model so that the model can pay more attention to the key features. The results show that the average accuracy and average F1-score of the multiscale feature fusion deep residual network on the test set are 93.81% and 94.44%, respectively, and the numbers of floating-point operations per second (FLOPs) and parameters are 5.95 G and 6.15 M, respectively. Compared to other deep residual networks, the multiscale feature fusion deep residual network achieves the highest classification accuracy. Therefore, the network proposed in this paper can classify needle-shaped seeds efficiently and provide a reference for seed recognition in agriculture. Full article
(This article belongs to the Special Issue In-Field Detection and Monitoring Technology in Precision Agriculture)
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<p>Four different seeds of <span class="html-italic">Bidens</span> L. (<b>a</b>) <span class="html-italic">B. Pilosa</span>. (<b>b</b>) <span class="html-italic">B. bipinnata</span>. (<b>c</b>) <span class="html-italic">B. pilosa var. radiata</span>. (<b>d</b>) <span class="html-italic">B. biternate</span>.</p>
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<p>Comparison of segmentation results. (<b>a</b>) Original image. (<b>b</b>) Threshold segmentation result. (<b>c</b>) Semantic segmentation result.</p>
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<p><span class="html-italic">Bidens</span> L. seed image segmentation and separation processing.</p>
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<p>Dataset sample distribution.</p>
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<p>MSFF-ResNet structure.</p>
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<p>Impact of the multiscale feature fusion module on feature extraction. (<b>a</b>) Original images. (<b>b</b>) Images obtained using the Grad-CAM of the feature extraction layer without the multiscale feature fusion block. (<b>c</b>) Images obtained using the Grad-CAM of the feature extraction layer with the multiscale feature fusion block.</p>
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<p>Impact of CBAM on feature extraction. (<b>a</b>) Original images. (<b>b</b>) Images obtained using Grad-CAM of the feature extraction layer without CBAM. (<b>c</b>) Images obtained using Grad-CAM of the feature extraction layer with CBAM.</p>
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<p>Impact of CBAM on feature extraction. (<b>a</b>) Original images. (<b>b</b>) Images obtained using Grad-CAM of the feature extraction layer without CBAM. (<b>c</b>) Images obtained using Grad-CAM of the feature extraction layer with CBAM.</p>
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<p>Training loss of different models.</p>
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<p>Validation accuracy of different models.</p>
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<p>Confusion matrix of MSFF-ResNet.</p>
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<p>Results of K-fold cross-validation experiment.</p>
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22 pages, 11077 KiB  
Article
MDD-DETR: Lightweight Detection Algorithm for Printed Circuit Board Minor Defects
by Jinmin Peng, Weipeng Fan, Song Lan and Dingran Wang
Electronics 2024, 13(22), 4453; https://doi.org/10.3390/electronics13224453 - 13 Nov 2024
Viewed by 308
Abstract
PCBs (printed circuit boards) are the core components of modern electronic devices, and inspecting them for defects will have a direct impact on the performance, reliability and cost of the product. However, the performance of current detection algorithms in identifying minor PCB defects [...] Read more.
PCBs (printed circuit boards) are the core components of modern electronic devices, and inspecting them for defects will have a direct impact on the performance, reliability and cost of the product. However, the performance of current detection algorithms in identifying minor PCB defects (e.g., mouse bite and spur) still requires improvement. This paper presents the MDD-DETR algorithm for detecting minor defects in PCBs. The backbone network, MDDNet, is used to efficiently extract features while significantly reducing the number of parameters. Simultaneously, the HiLo attention mechanism captures both high- and low-frequency features, transmitting a broader range of gradient information to the neck. Additionally, the proposed SOEP neck network effectively fuses scale features, particularly those rich in small targets, while INM-IoU loss function optimization enables more effective distinction between defects and background, further improving detection accuracy. Experimental results on the PCB_DATASET show that MDD-DETR achieves a 99.3% mAP, outperforming RT-DETR by 2.0% and reducing parameters by 32.3%, thus effectively addressing the challenges of detecting minor PCB defects. Full article
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<p>RT-DETR network structure.</p>
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<p>MDD-DETR network structure.</p>
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<p>MDD block.</p>
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<p>HiLo attention framework.</p>
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<p>The IoU calculation factor.</p>
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<p>The MPDIoU calculation factor.</p>
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<p>The architecture of CSPOK. FFT and IFFT represent the fast Fourier transform and its inverse operation, respectively.</p>
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<p>CREC module.</p>
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<p>Visualization comparison: (<b>a</b>) original; (<b>b</b>) baseline; (<b>c</b>) MDD-DETR.</p>
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<p>Visualization comparison: (<b>a</b>) original; (<b>b</b>) baseline; (<b>c</b>) MDD-DETR.</p>
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<p>Comparison of heat maps for detection performance: (<b>a</b>) original; (<b>b</b>) baseline; (<b>c</b>) MDD-DETR.</p>
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28 pages, 45529 KiB  
Article
High-Quality Damaged Building Instance Segmentation Based on Improved Mask Transfiner Using Post-Earthquake UAS Imagery: A Case Study of the Luding Ms 6.8 Earthquake in China
by Kangsan Yu, Shumin Wang, Yitong Wang and Ziying Gu
Remote Sens. 2024, 16(22), 4222; https://doi.org/10.3390/rs16224222 - 13 Nov 2024
Viewed by 344
Abstract
Unmanned aerial systems (UASs) are increasingly playing a crucial role in earthquake emergency response and disaster assessment due to their ease of operation, mobility, and low cost. However, post-earthquake scenes are complex, with many forms of damaged buildings. UAS imagery has a high [...] Read more.
Unmanned aerial systems (UASs) are increasingly playing a crucial role in earthquake emergency response and disaster assessment due to their ease of operation, mobility, and low cost. However, post-earthquake scenes are complex, with many forms of damaged buildings. UAS imagery has a high spatial resolution, but the resolution is inconsistent between different flight missions. These factors make it challenging for existing methods to accurately identify individual damaged buildings in UAS images from different scenes, resulting in coarse segmentation masks that are insufficient for practical application needs. To address these issues, this paper proposed DB-Transfiner, a building damage instance segmentation method for post-earthquake UAS imagery based on the Mask Transfiner network. This method primarily employed deformable convolution in the backbone network to enhance adaptability to collapsed buildings of arbitrary shapes. Additionally, it used an enhanced bidirectional feature pyramid network (BiFPN) to integrate multi-scale features, improving the representation of targets of various sizes. Furthermore, a lightweight Transformer encoder has been used to process edge pixels, enhancing the efficiency of global feature extraction and the refinement of target edges. We conducted experiments on post-disaster UAS images collected from the 2022 Luding earthquake with a surface wave magnitude (Ms) of 6.8 in the Sichuan Province of China. The results demonstrated that the average precisions (AP) of DB-Transfiner, APbox and APseg, are 56.42% and 54.85%, respectively, outperforming all other comparative methods. Our model improved the original model by 5.00% and 4.07% in APbox and APseg, respectively. Importantly, the APseg of our model was significantly higher than the state-of-the-art instance segmentation model Mask R-CNN, with an increase of 9.07%. In addition, we conducted applicability testing, and the model achieved an average correctness rate of 84.28% for identifying images from different scenes of the same earthquake. We also applied the model to the Yangbi earthquake scene and found that the model maintained good performance, demonstrating a certain level of generalization capability. This method has high accuracy in identifying and assessing damaged buildings after earthquakes and can provide critical data support for disaster loss assessment. Full article
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<p>The study area and UAS orthophotos after the earthquake in Luding County, Sichuan Province. (<b>A</b>) study area; (<b>B</b>) UAS orthophotos: (<b>a</b>,<b>c</b>) Moxi town; (<b>b</b>,<b>d</b>,<b>g</b>) Detuo town; (<b>e</b>) Fawang village; (<b>f</b>) Wandong village.</p>
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<p>The samples of damaged buildings and labels: (<b>a</b>) Field investigation photos; (<b>b</b>) UAS images, the red fan-shaped marker representing the viewing angle of the observation location; (<b>c</b>) Labeled bounding boxes; (<b>d</b>) Labeled instance masks, the color of the polygon masks represents different instance objects.</p>
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<p>The network architecture of Mask Transfiner.</p>
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<p>The improved network architecture for DB-Transfiner. Deformable convolution is employed in the backbone. The FPN is replaced by enhanced BiFPN to fuse the multi-scale features, and, in this study, a lightweight sequence encoder is adopted for efficiency.</p>
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<p>Deformable convolution feature extraction module. Arrows indicate the type of convolution used at each stage. The first two stages use standard convolution, and the last three stages use deformable convolution. (<b>a</b>) Standard convolution; (<b>b</b>) Deformable convolution.</p>
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<p>Replacing FPN with enhanced BiFPN to improve feature fusion network.</p>
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<p>Lightweight sequence encoder to improve the efficiency of the network, using a Transformer structure with an eight-headed self-attention mechanism instead of three Transformer structures with four-headed self-attention mechanisms.</p>
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<p>Loss curve during DB-Transfiner training.</p>
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<p>Comparison of the performance of all models based on the metrics <span class="html-italic">AP</span> (%).</p>
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<p>Visualization of the prediction results of different network models. The colored bounding boxes and polygons represent the detection and segmentation results, respectively. (<b>a</b>) Annotated images; (<b>b</b>) Mask R-CNN; (<b>c</b>) Mask Transfiner; (<b>d</b>) DB-Transfiner.</p>
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<p>Visualization of instance mask results of different network models. The colored polygons represent the recognized instance objects. ① and ② represent two typical damaged buildings with the same level of destruction. (<b>a</b>) Original images; (<b>b</b>) Annotated results; (<b>c</b>) Mask R-CNN; (<b>d</b>) Mask Transfiner; (<b>e</b>) DB-Transfiner.</p>
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<p>Visualization of heatmaps: (<b>a</b>) The original images; (<b>b</b>) The heatmaps of Conv2_x layer of the DCNM; (<b>c</b>) The heatmaps of Conv5_x layer of the DCNM; (<b>d</b>) The heatmaps of <math display="inline"><semantics> <mrow> <msub> <mrow> <mi>N</mi> </mrow> <mrow> <mn>5</mn> </mrow> </msub> </mrow> </semantics></math> layer of the MEFM; (<b>e</b>) The final results. The colored borders represent the model’s predicted different instance objects.</p>
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<p>The visualization of feature maps before and after the LTGM. The colored borders represent the different instance objects.</p>
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<p>Results of damaged building classification in Fawang village (<a href="#remotesensing-16-04222-f001" class="html-fig">Figure 1</a>B(e)). Red indicates correct detections, green indicates incorrect detections, and yellow indicates missed.</p>
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<p>Results of damaged building classification in Wandong village and Detuo town (<a href="#remotesensing-16-04222-f001" class="html-fig">Figure 1</a>B(f,g)). Red indicates correct detections, green indicates incorrect detections, and yellow indicates missed.</p>
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<p>Example of UAV imagery from the Yangbi earthquake in Yunnan, China: (<b>a</b>) Huaian village; (<b>b</b>) Yangbi town.</p>
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<p>UAS imagery samples of damaged buildings from the Yangbi earthquake. (<b>a</b>) The red irregular polygons denote the damaged buildings. (<b>b</b>) The bounding boxes and polygon masks are the visualized results of our model. The colors represent different instance objects.</p>
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<p>Examples of densely built-up areas. The red boxes indicate buildings with blurred contour information caused by shadows and occlusions.</p>
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18 pages, 3875 KiB  
Article
Spatiotemporal Dynamics of Water Quality: Long-Term Assessment Using Water Quality Indices and GIS
by Dániel Balla, Emőke Kiss, Marianna Zichar and Tamás Mester
ISPRS Int. J. Geo-Inf. 2024, 13(11), 408; https://doi.org/10.3390/ijgi13110408 - 12 Nov 2024
Viewed by 374
Abstract
The severe contamination of groundwater supplies in rural areas is a global problem that requires strict environmental measures. Related to this, one of the most important challenges at present is the elimination of local sources of pollution. Therefore, this research examined the local [...] Read more.
The severe contamination of groundwater supplies in rural areas is a global problem that requires strict environmental measures. Related to this, one of the most important challenges at present is the elimination of local sources of pollution. Therefore, this research examined the local water quality changes following the construction of the sewerage network, under the framework of long-term monitoring (2011–2022) in Báránd, Hungary, using water quality indices and GIS (Geographic Information System) techniques. In order to understand the purification processes and spatial and temporal changes, three periods were determined: the pre-sewerage period (2011–2014), the transitional period (2015–2018), and the post-sewerage period (2019–2022). Forty monitoring wells were included in the study, ensuring complete coverage of the municipality. The results revealed a high level of pollution in the area in the pre-sewerage period. Based on the calculated indices, an average of 80% of the wells were ranked in categories 4–5, indicating poor water quality, while less than 8% were classified in categories 1–2, indicating good water quality. No significant purification process was detected in the transitional period. However, marked changes were observed in the post-sewerage period as a result of the elimination of local sources of pollution. In the post-sewerage period, the number of monitoring wells ranked as excellent and good increased significantly. Additionally, the number of wells assigned to category 5 decreased markedly, compared to the reference period. The significant difference between the three periods was confirmed by the Wilcoxon test as well (p < 0.05). Based on interpolated maps, it was found that, in the post-sewerage period, an increasing section of the settlement had good or excellent water quality. In addition to an assessment of long-term tendencies, the annual fluctuations in the water quality of the wells were also examined. This showed that the purification processes do not occur in a linear pattern but are influenced by various factors (e.g., precipitation). Our results highlight the importance of protecting and improving groundwater resources in municipal areas and the relevance of long-term monitoring of water adequate management policy. Full article
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<p>The study area, water sampling sites, and the process of data collection and preprocessing.</p>
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<p>WQI and Cd index values between 2011 and 2022.</p>
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<p>Number of categories according to WQI and Cd.</p>
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<p>Index values in the investigated periods.</p>
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<p>Thematic point map of the spatial distribution of WQI and Cd between 2011 and 2022.</p>
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<p>Time-series map of the spatial distribution of WQI and Cd in the investigated periods (2011–2022).</p>
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<p>Difference rank maps of the indices (WQS and Cd) between 2011 and 2022.</p>
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<p>Water quality rank changes based on WQI and Cd ranks of the monitoring wells.</p>
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<p>Water quality rank changes, based on WQI, in the monitoring wells between 2011 and 2022.</p>
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<p>Water quality rank changes, based on Cd, in the monitoring wells between 2011 and 2022.</p>
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21 pages, 4646 KiB  
Article
Analysis of Quantum-Classical Hybrid Deep Learning for 6G Image Processing with Copyright Detection
by Jongho Seol, Hye-Young Kim, Abhilash Kancharla and Jongyeop Kim
Information 2024, 15(11), 727; https://doi.org/10.3390/info15110727 - 12 Nov 2024
Viewed by 449
Abstract
This study investigates the integration of quantum computing, classical methods, and deep learning techniques for enhanced image processing in dynamic 6G networks, while also addressing essential aspects of copyright technology and detection. Our findings indicate that quantum methods excel in rapid edge detection [...] Read more.
This study investigates the integration of quantum computing, classical methods, and deep learning techniques for enhanced image processing in dynamic 6G networks, while also addressing essential aspects of copyright technology and detection. Our findings indicate that quantum methods excel in rapid edge detection and feature extraction but encounter difficulties in maintaining image quality compared to classical approaches. In contrast, classical methods preserve higher image fidelity but struggle to satisfy the real-time processing requirements of 6G applications. Deep learning techniques, particularly CNNs, demonstrate potential in complex image analysis tasks but demand substantial computational resources. To promote the ethical use of AI-generated images, we introduce copyright detection mechanisms that employ advanced algorithms to identify potential infringements in generated content. This integration improves adherence to intellectual property rights and legal standards, supporting the responsible implementation of image processing technologies. We suggest that the future of image processing in 6G networks resides in hybrid systems that effectively utilize the strengths of each approach while incorporating robust copyright detection capabilities. These insights contribute to the development of efficient, high-performance image processing systems in next-generation networks, highlighting the promise of integrated quantum-classical–classical deep learning architectures within 6G environments. Full article
(This article belongs to the Section Information Applications)
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<p>First simulation of comparative results of the quantum and classical models in our 6G network simulation.</p>
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<p>Second comparative results of the quantum and classical models in our 6G network simulation.</p>
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<p>Execution times for each simulation with averages.</p>
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<p>Average execution time comparison between quantum and classical (100 simulations).</p>
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<p>Average expected cost over time between quantum and classical models.</p>
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<p>Execution time, speedup factor, average cost, and cost ratio vs. quantum advantage.</p>
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<p>Image processing execution time, speedup factor, average cost, and cost ratio vs. quantum advantage.</p>
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<p>Sample images for 6G network simulation.</p>
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<p>Quantum image processing results in 6G network.</p>
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<p>Classical image processing results in 6G network.</p>
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<p>PSNR comparison: quantum vs. classical image processing in 6G network.</p>
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12 pages, 2052 KiB  
Article
6G Technology for Indoor Localization by Deep Learning with Attention Mechanism
by Chien-Ching Chiu, Hung-Yu Wu, Po-Hsiang Chen, Chen-En Chao and Eng Hock Lim
Appl. Sci. 2024, 14(22), 10395; https://doi.org/10.3390/app142210395 - 12 Nov 2024
Viewed by 330
Abstract
This paper explores 6G technology for indoor positioning, focusing on accuracy and reliability using convolutional neural networks (CNN) with channel state information (CSI). Indoor positioning is critical for smart applications and the Internet of Things (IoT). 6G is expected to significantly enhance positioning [...] Read more.
This paper explores 6G technology for indoor positioning, focusing on accuracy and reliability using convolutional neural networks (CNN) with channel state information (CSI). Indoor positioning is critical for smart applications and the Internet of Things (IoT). 6G is expected to significantly enhance positioning performance through the use of higher frequency bands, such as terahertz frequencies with wider bandwidth. Preliminary results show that 6G-based systems are expected to achieve centimeter-level positioning accuracy due to the integration of advanced artificial intelligence algorithms and terahertz frequencies. In addition, this paper also investigates the impact of self-attention (SA) and channel attention (CA) mechanisms on indoor positioning systems. The combination of these attention mechanisms with conventional CNNs has been proposed to further improve the accuracy and robustness of localization systems. CNN with SA demonstrates a 50% reduction in RMSE compared to CNN by capturing spatial dependencies more effectively. Full article
(This article belongs to the Special Issue 5G and Beyond: Technologies and Communications)
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<p>Blocks of the indoor positioning system, with an offline phase for model training on preprocessed data and an online phase for real-time location estimation.</p>
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<p>CNN architectures with multiple 3 × 3 convolutional layers, pooling layers, softmax layer (attention mechanism), and fully connected layers for localization.</p>
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<p>SA module with 1 <math display="inline"><semantics> <mrow> <mo>×</mo> </mrow> </semantics></math> 1 convolutional layers for feature extraction and attention weight calculation.</p>
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<p>CA module with several convolutional layers to extract channel-specific features and attention weights for enhanced feature representation.</p>
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<p>Floor plan of simulation environment: (<b>a</b>) two bookcases, three tables, and three transmitting antennas. (<b>b</b>) Layout of the 289 (17 <math display="inline"><semantics> <mrow> <mo>×</mo> </mrow> </semantics></math> 17) receiving antennas within a 10 <math display="inline"><semantics> <mrow> <mo>×</mo> </mrow> </semantics></math> 10 m environment. Each red dot represents a receiving antenna.</p>
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<p>RMSE versus epoch for all the LOS waves (three Txs scenario).</p>
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20 pages, 3450 KiB  
Article
Rheology of Cellulosic Microfiber Suspensions Under Oscillatory and Rotational Shear for Biocomposite Applications
by Helena Cristina Vasconcelos, Henrique Carrêlo, Telmo Eleutério, Maria Gabriela Meirelles, Reşit Özmenteş and Roberto Amorim
Compounds 2024, 4(4), 688-707; https://doi.org/10.3390/compounds4040042 - 12 Nov 2024
Viewed by 241
Abstract
This study investigates the rheological behavior of cellulose microfiber suspensions derived from kahili ginger stems (Hedychium gardnerianum), an invasive species, in two adhesive matrices: a commercial water-based adhesive (Coplaseal®) and a casein-based adhesive made from non-food-grade milk, referred to [...] Read more.
This study investigates the rheological behavior of cellulose microfiber suspensions derived from kahili ginger stems (Hedychium gardnerianum), an invasive species, in two adhesive matrices: a commercial water-based adhesive (Coplaseal®) and a casein-based adhesive made from non-food-grade milk, referred to as K and S samples, respectively. Rheological analyses were performed using oscillatory and rotational shear tests conducted at 25 °C, 50 °C, and 75 °C to assess the materials’ viscoelastic properties more comprehensively. Oscillatory tests across a frequency range of 1–100 rad/s assessed the storage modulus (G′) and loss modulus (G″), while rotational shear tests evaluated apparent viscosity and shear stress across shear rates from 0.1 to 1000 s−1. Fiber-free samples consistently showed lower moduli than fiber-containing samples at all frequencies. The incorporation of fibers increased the dynamic moduli in both K and S samples, with a quasi-plateau observed at lower frequencies, suggesting solid-like behavior. This trend was consistent in all tested temperatures. As frequencies increased, the fiber network was disrupted, transitioning the samples to fluid-like behavior, with a marked increase in G′ and G″. This transition was more pronounced in K samples, especially above 10 rad/s at 25 °C and 50 °C, but less evident at 75 °C. This shift from solid-like to fluid-like behavior reflects the transition from percolation effects at low frequencies to matrix-dominated responses at high frequencies. In contrast, S samples displayed a wider frequency range for the quasi-plateau, with less pronounced moduli changes at higher frequencies. At 75 °C, the moduli of fiber-containing and fiber-free S samples nearly converged at higher frequencies, indicating similar effects of the fiber and matrix components. Both fiber-reinforced and non-reinforced suspensions exhibited pseudoplastic (shear-thinning) behavior. Fiber-containing samples exhibited higher initial viscosity, with K samples displaying greater differences between fiber-reinforced and non-reinforced systems compared to S samples, where the gap was narrower. Interestingly, S samples exhibited overall higher viscosity than K samples, implying a reduced influence of fibers on the viscosity in the S matrix. This preliminary study highlights the complex interactions between cellulosic fiber networks, adhesive matrices, and rheological conditions. The findings provide a foundation for optimizing the development of sustainable biocomposites, particularly in applications requiring precise tuning of rheological properties. Full article
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<p>SEM micrograph of mechanically extracted fiber from the stems of the Kahili ginger plant (<span class="html-italic">Hedychium gardnerianum</span>).</p>
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<p>(<b>a</b>) Long <span class="html-italic">Hedychium gardnerianum</span> fibers (&gt;10 cm); (<b>b</b>) short <span class="html-italic">Hedychium gardnerianum</span> fibers (between 0.2 and 5 mm).</p>
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<p>Oscillatory tests for k0 and k10 at different temperatures: (<b>a</b>) 25 °C, (<b>b</b>) 50 °C, and (<b>c</b>) 75 °C. Each graph, with logarithmic scales on both axes, displays storage modulus (G′) and loss modulus (G″) as functions of angular frequency (ω) in rad/s.</p>
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<p>Comparison of the storage modulus (G′) and loss modulus (G″) of Kappa samples across different temperatures (25, 50, and 75 °C). Panel (<b>a</b>) shows data for Kappa samples with fibers (k10), while panel (<b>b</b>) displays data for Kappa samples without fibers (k0). Note: Error bars are not plotted here for simplicity.</p>
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<p>Oscillatory tests for s0 and s10 at different temperatures: (<b>a</b>) 25 °C, (<b>b</b>) 50 °C, and (<b>c</b>) 75 °C. Each graph, with logarithmic scales on both axes, displays the storage modulus (G′) and the loss modulus (G″) as functions of angular frequency (ω) in rad/s.</p>
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<p>Comparison of the storage modulus (G′) and loss modulus (G″) of Super samples across different temperatures (25, 50, and 75 °C). Panel (<b>a</b>) shows data for Super samples with fibers (s10), while the panel (<b>b</b>) displays data for Super samples without fibers (s0). Note: Error bars are not plotted here for simplicity.</p>
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<p>Continuous tests of K samples, without fibers and with 10% fibers, at different temperatures (25 °C, 50 °C, and 75 °C): Apparent viscosity (<b>a</b>); shear stress (<b>b</b>).</p>
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<p>End of continuous test for k25,10. Expulsion of the sample from the gap is observed.</p>
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<p>Post-rheological test observations of K samples at different temperatures: (<b>a</b>) Sample at 25 °C showing a liquid state without significant thickening; (<b>b</b>) sample at 50 °C with visible thick layer formation beginning to occur; (<b>c</b>) sample at 75 °C showing substantial film formation along edge zones; (<b>d</b>) another view of 75 °C sample, highlighting more pronounced thick layers at edges; (<b>e</b>) sample tested at 75 °C showing reduced volume after testing due to thick layer formation.</p>
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<p>Continuous tests S samples, without fibers and with 10% fibers at different temperatures (25, 50, and 75 °C): (<b>a</b>) viscosity; (<b>b</b>) shear stress.</p>
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<p>(<b>a</b>) Fiber-free S samples at 25 °C after rotational test with gap leak; (<b>b</b>) fiber-free S samples at 75 °C following oscillatory test; (<b>c</b>) fiber-free S samples at 50 °C after rotational testing.</p>
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<p>S sample with fibers after an oscillatory test at 50 °C.</p>
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36 pages, 17228 KiB  
Article
Anti-Obesity Effects of Adzuki Bean Saponins in Improving Lipid Metabolism Through Reducing Oxidative Stress and Alleviating Mitochondrial Abnormality by Activating the PI3K/Akt/GSK3β/β-Catenin Signaling Pathway
by Jinhai Luo, Jincan Luo, Yingzi Wu, Yu Fu, Zhonghao Fang, Bincheng Han, Bin Du, Zifeng Yang and Baojun Xu
Antioxidants 2024, 13(11), 1380; https://doi.org/10.3390/antiox13111380 - 11 Nov 2024
Viewed by 695
Abstract
Obesity is a chronic and complex disease defined by the excessive deposition of fat and is highly associated with oxidative stress. Adzuki bean saponins (ABS) showed anti-obesity activity in our previous in vivo study; however, the active saponins of adzuki beans and potential [...] Read more.
Obesity is a chronic and complex disease defined by the excessive deposition of fat and is highly associated with oxidative stress. Adzuki bean saponins (ABS) showed anti-obesity activity in our previous in vivo study; however, the active saponins of adzuki beans and potential mechanisms are still unclear. This research aims to elucidate the anti-obesity effects of ABS in improving lipid metabolism and oxidative stress, exploring the effective ingredients and potential molecular mechanisms through UHPLC-QE-MS analysis, network pharmacology, bioinformatics, and in vitro experiments both in the 3T3-L1 cell line and HepG2 cell line. The results indicate that ABS can improve intracellular lipid accumulation, adipogenesis, oxidative stress, and mitochondrial damage caused by lipid accumulation including ROS generation, abnormal mitochondrial membrane potential, and ATP disorder. Fifteen saponin components were identified with the UHPLC-QE-MS analysis. The network pharmacology and bioinformatics analyses indicated that the PI3K/Akt signaling pathway is associated with the bioactive effect of ABS. Through Western blotting and immunofluorescence analysis, the anti-obesity effect of ABS is achieved through regulation of the PI3K/Akt/GSK3β/β-catenin signaling pathway and activation of downstream transcription factor c-Myc in the lipid accumulation cell model, and regulation of β-catenin signaling and inhibition of downstream transcription factor C/EBPα in the adipocyte cell model. These results illustrate the biological activity of ABS in improving fat metabolism and oxidative stress by restoring mitochondrial function through β-catenin signaling, the PI3K/Akt/GSK3β/β-catenin signaling pathway, laying the foundation for its further development. Full article
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<p>The network pharmacology analysis of ABS. (<b>A</b>) DEGs screening and presentation as Volcano plots. (<b>B</b>) The Venn diagram between the drug targets, obesity targets, and lipid metabolism. (<b>C</b>) The PPI network of the interacting targets. (<b>D</b>) The drug–compounds–targets–disease network. (<b>E</b>) The dot plot of KEGG pathway enrichment analysis. (<b>F</b>) The dot plot of KEGG signaling pathway enrichment analysis. (<b>G</b>) The dot plot of GO enrichment analysis. BP, biological process; CC, cellular component; MF, molecular function.</p>
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<p>Selection of characteristic genes of intersection genes. (<b>A</b>) Ten-time cross-verification of tuning parameters in the LASSO model. Each curve refers to one gene. Solid vertical lines indicate the standard error (SE) of the partial likelihood deviation and dotted vertical line marks the optimal lambda value. (<b>B</b>) In the LASSO coefficient profile, adjustment of feature selection in the minimum absolute shrinkage and selection operator model. (<b>C</b>) The SVM-RFE algorithm was used for selecting features. (<b>D</b>) Examining the correlation between the count of trees in a random forest and the error rate. The red dotted line indicates the Training Error, which is how the model behaves on the training set. The black implementation indicates the Validation Error, which is how the model behaves on the validation set. The green dotted line indicates the Test Error, which is how the model behaves on the test set (<b>E</b>) Ranking of genes based on their relative importance. (<b>F</b>) A Venn diagram depicting genes common to the LASSO, random forest, and SVM-RFE methods.</p>
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<p>Immune infiltration study of character genes. (<b>A</b>) demonstrates a robust correlation among 11 genes, where red denotes a positive correlation, blue indicates a negative correlation, and “*” signifies a statistically significant correlation. Red color represents positive correlation, blue color represents negative correlation, and “*” indicates statistically correlation (<b>B</b>) illustrates variations in immune cell infiltration across different samples. (<b>C</b>) shows the disparity in immune cell infiltration between groups with and without obesity, which was statistically assessed, with “ns” indicating that the difference was statistically insignificant. (<b>D</b>) indicates the difference in immune cell infiltration between the groups with and without obesity.</p>
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<p>Diagnostic potential of characteristic genes in obesity. (<b>A</b>–<b>K</b>) ROC curves estimating the diagnostic performance of characteristic genes. (<b>A</b>) ABCC1, (<b>B</b>) ACACB, (<b>C</b>) F13A1, (<b>E</b>) FGF1, (<b>F</b>) FGF2, (<b>G</b>) HSD11B1, (<b>H</b>) PDE3A, (<b>I</b>) PTPN22, (<b>J</b>) MMP9 have better diagnostic potential for obesity.</p>
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<p>The anti-obesity effect of ABS on HepG2 cells. (<b>A</b>) The effect of ABS on the cell viability of HepG2 cells. *** <span class="html-italic">p</span> &lt; 0.001. (<b>B</b>) Effect of ABS on the triglyceride (TG) content in HepG2 cells. (<b>C</b>) Oil Red O (ORO) staining of free fatty acid (FFA)-induced HepG2 cellular model with or without treatment of adzuki bean saponins (ABS) at different doses. Figures are captured through scanning system of VS200 slide scanner system (Olypums, Tokyo, Japan) with a 40× objective. Scale bar, 50 μm. All results are presented as mean ± S.D and the experiments were repeated as triplicates. Bars with different letters (a–d) significantly (<span class="html-italic">p</span> &lt; 0.05) differ according to ANOVA and Tukey’s multiple range test. FFA, free fatty acid; ABS, adzuki bean saponins.</p>
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<p>ABS alleviates FFA-mediated lipid aggregation. (<b>A</b>) Fluorescent images of HepG2 cells co-stained with Hochest 33342 (blue) and Nile Red (green and red) taken by ZEISS Elyra 7 with Lattice structured illumination microscopy (SIM)<sup>2</sup> super-resolution fluorescent microscope equipped with a 63× oil-immersion objective Red Arrow: Fluorescent aggregation with strong green and red signal. Scale bars, 5 μm. (<b>B</b>) Semi-quantitative analysis based on original images taken by SIM<sup>2</sup> of fluorescence signal and lipid droplet by Image J. All results are presented as mean ± S.D and the experiments were repeated as triplicates. Bars with different letters (a–d) significantly (<span class="html-italic">p</span> &lt; 0.05) differ according to ANOVA and Tukey’s multiple range test. FFA, free fatty acid; ABS, adzuki bean saponins.</p>
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<p>Quantitative phase imaging (QPI) of 24-hour observation (captured every 0.5 hours) of FFA-induced model with or without treatment of ABS performed using a Livecyte2 microscope with a 10× objective. Control is the HepG2 cell treated with the MEM medium; model is the HepG2 cell treated with the MEM medium containing the free fatty acid; ABS 0.2 mg/mL is the HepG2 cell treated with the MEM medium containing the free fatty acid and 0.2 mg/mL dosage of the adzuki bean saponins. Blue Arrow: Time-lapse change of single cell with fluorescence re-distribution.</p>
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<p>ABS alleviates FFA-facilitated intracellular ROS accumulation. (<b>A</b>) Fluorescent images of HepG2 cells co-stained with Hochest 33342 (blue) and Carboxy-H2DCFDA (green) taken by ZEISS Elyra 7 equipped with Lattice structured illumination microscopy (SIM)<sup>2</sup> super-resolution fluorescent microscope applying a 10× objective. Scale bars, 50 μm. Red Arrow: Fluorescent aggregation with strong green signal (<b>B</b>) Semi-quantitative analysis based on original images taken by SIM<sup>2</sup> of fluorescence signal of ROS intensity by Image J. (<b>C</b>) Normalized relative fluorescent unit (RFU) representing ROS accumulation (Carboxy-H<sub>2</sub>DCFDA) in bulk culture in 96-well plates, which is divided by the cell number. All results are presented as mean ± S.D and the experiments were repeated as triplicates. Bars with different letters (a–b) significantly (<span class="html-italic">p</span> &lt; 0.05) differ according to ANOVA and Tukey’s multiple range test. FFA, free fatty acid; ABS, adzuki bean saponins.</p>
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<p>ABS alleviates FFA-facilitated mitochondrial membrane potential loss and ATP level abnormality. (<b>A</b>) Fluorescent images of HepG2 cells co-stained with Hochest 33,342 (blue) and DiBaC<sub>4</sub> (green) taken by ZEISS Elyra 7 equipped with Lattice structured illumination microscopy (SIM)<sup>2</sup> super-resolution fluorescent microscope with a 63× oil-immersion objective. Scale bars, 5 μm. Red Arrow: Fluorescent aggregation with strong green and blue signal. (<b>B</b>) Fluorescent images of HepG2 cells co-stained with Hochest 33,342 (blue) and JC-1 (green or red) taken by ZEISS Elyra 7 equipped with Lattice structured illumination microscopy (SIM)<sup>2</sup> super-resolution fluorescent microscope with a 63× oil-immersion objective. Scale bars, 5 μm. (<b>C</b>) Normalized relative fluorescent unit (RFU) representing membrane potential change (DiBaC<sub>4</sub>) in bulk culture in 96-well plates, which is divided by the cell number. (<b>D</b>) Semi-quantitative analysis based on original images taken by SIM<sup>2</sup> of fluorescence signal of JC-1 intensity by Image J. (<b>E</b>) Normalized relative luminescent unit (RLU) representing ATP level in bulk culture in 96-well plates, which is divided by the cell number. All results are presented as mean ± S.D, and the experiments were repeated as triplicates. Bars with different letters (a–c) significantly (<span class="html-italic">p</span> &lt; 0.05) differ according to ANOVA and Tukey’s multiple range test. FFA, free fatty acid; ABS, adzuki bean saponins.</p>
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<p>The regulation effect of ABS on the PI3K/Akt/GSK3β/β-catenin signaling pathway. (<b>A</b>) The PI3K/Akt/GSK3β signaling pathway-related proteins and phosphorylated proteins. (<b>B</b>) The protein level of p-PI3K/PI3K. (<b>C</b>) The protein level of p-Akt/Akt. (<b>D</b>) The protein level of p-GSK3β/GSK3β. (<b>E</b>) The proteins of β-catenin, its downstream transcriptional factor c-Myc, and GAPDH. (<b>F</b>) The protein level of β-catenin/GAPDH. (<b>G</b>) The protein level of c-Myc/GAPDH. All results are presented as mean ± S.D and the experiments were repeated as triplicates. Bars with different letters (a–c) significantly (<span class="html-italic">p</span> &lt; 0.05) differ according to ANOVA and Tukey’s multiple range test. FFA, free fatty acid; ABS, adzuki bean saponins.</p>
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<p>The regulation effect of ABS on nuclear translocation of β-catenin. (<b>A</b>) Fluorescent images of HepG2 cells containing immuno-fluorescent signal of β-catenin (green) and counter-stained with DAPI (blue), taken by ZEISS Elyra 7 equipped with Lattice structured illumination microscopy SIM<sup>2</sup> super-resolution fluorescent microscope with a 63× oil-immersion objective. Scale bars, 5 μm. (<b>B</b>) Semi-quantitative analysis based on original images taken by SIM<sup>2</sup> of relative green fluorescence signal (β-catenin), normalized by dividing to blue fluorescence signal (DAPI) by Image J. (<b>C</b>) The nuclear proteins of β-catenin and Lamin B1. (<b>D</b>) The relative protein levels of β-catenin/Lamin B1. All results are presented as mean ± S.D and the experiments were repeated as triplicates. Bars with different letters (a,b) significantly (<span class="html-italic">p</span> &lt; 0.05) differ according to ANOVA and Tukey’s multiple range test. FFA, free fatty acid; ABS, adzuki bean saponins.</p>
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<p>(<b>A</b>) The effect of ABS on the cell viability of 3T3-L1 cells. (<b>B</b>) The effect of EGCG on the cell viability of 3T3-L1 cells. **** <span class="html-italic">p</span> &lt; 0.0001. All results are presented as mean ± S.D and the experiments were repeated as triplicates. EGCG, epigallocatechin gallate.</p>
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<p>ABS alleviates MDI-induced adipogenesis. (<b>A</b>) Fluorescent images of 3T3-L1 cells co-stained with Hochest 33,342 (blue) and Nile Red (green and red) taken by ZEISS Elyra 7 with Lattice structured illumination microscopy (SIM)<sup>2</sup> super-resolution fluorescent microscope equipped with a 63× oil-immersion objective. Scale bars, 5 μm. (<b>B</b>) Semi-quantitative analysis based on original images taken by SIM<sup>2</sup> of fluorescence signal and lipid droplet by Image J. (<b>C</b>) ORO staining of MDI-induced 3T3-L1 cellular model with or without treatment of ABS or EGCG. All results are presented as mean ± S.D and the experiments were repeated as triplicates. Bars with different letters (a–e) significantly (<span class="html-italic">p</span> &lt; 0.05) differ according to ANOVA and Tukey’s multiple range test. Scale bar, 50 μm. MDI, adipogenesis differentiation medium; EGCG, epigallocatechin gallate; ABS, adzuki bean saponins.</p>
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<p>The regulation effect of ABS on protein level of β-catenin. (<b>A</b>) Fluorescent images of 3T3-L1 cells containing immuno-fluorescent signal of β-catenin (green) and counter-stained with DAPI (blue) taken by ZEISS Elyra 7 equipped with Lattice structured illumination microscopy (SIM)<sup>2</sup> super-resolution fluorescent microscope with a 63× oil-immersion objective. Scale bars, 5 μm. MDI, adipogenesis differentiation medium; ABS, adzuki bean saponins. (<b>B</b>) Semi-quantitative analysis based on original images taken by SIM<sup>2</sup> of relative green fluorescence signal (β-catenin), normalized by dividing to blue fluorescence signal (DAPI) by Image J. Bars with different letters (a–c) significantly (<span class="html-italic">p</span> &lt; 0.05) differ according to ANOVA and Tukey’s multiple range test.</p>
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<p>The regulation effect of ABS on protein level of transcriptional factor C/EBPα and PPARγ. (<b>A</b>) Fluorescent images of 3T3-L1 cells containing immuno-fluorescent signal of CEBP/α (green) and counter-stained with DAPI (blue) taken by ZEISS Elyra 7 equipped with Lattice structured illumination microscopy (SIM)<sup>2</sup> super-resolution fluorescent microscope with a 63× oil-immersion objective. Scale bars, 5 μm. (<b>B</b>) Semi-quantitative analysis based on original images taken by SIM<sup>2</sup> of relative green fluorescence signal (CEBP/α), normalized by dividing to blue fluorescence signal (DAPI) by Image J. (<b>C</b>) Fluorescent images of 3T3-L1 cells containing immuno-fluorescent signal of PPARγ (green) and counter-stained with DAPI (blue) taken by ZEISS Elyra 7 equipped with Lattice structured illumination microscopy (SIM)<sup>2</sup> super-resolution fluorescent microscope with a 63× oil-immersion objective. Scale bars, 10 μm. (<b>D</b>) Semi-quantitative analysis based on original images taken by SIM<sup>2</sup> of relative green fluorescence signal (PPARγ), normalized by dividing to blue fluorescence signal (DAPI) by Image J. All results are presented as mean ± S.D and the experiments were repeated as triplicates. Bars with different letters (a–c) significantly (<span class="html-italic">p</span> &lt; 0.05) differ according to ANOVA and Tukey’s multiple range test. MDI, adipogenesis differentiation medium; ABS, adzuki bean saponins.</p>
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13 pages, 2322 KiB  
Article
Artificial Neural Network Modeling to Predict Electrical Conductivity and Moisture Content of Milk During Non-Thermal Pasteurization: New Application of Artificial Intelligence (AI) in Food Processing
by Ali Wali M. Alsaedi, Asaad R. Al-Hilphy, Azhar J. Al-Mousawi and Mohsen Gavahian
Processes 2024, 12(11), 2507; https://doi.org/10.3390/pr12112507 - 11 Nov 2024
Viewed by 503
Abstract
This study proposed applying artificial intelligence (AI) to predict the actual electrical conductivity (EC) of raw and pasteurized milk using moderate electric field (MEF) based on the electric field strength (EFS) and mass flow rate (MFR) along with modeling moisture content (MC) based [...] Read more.
This study proposed applying artificial intelligence (AI) to predict the actual electrical conductivity (EC) of raw and pasteurized milk using moderate electric field (MEF) based on the electric field strength (EFS) and mass flow rate (MFR) along with modeling moisture content (MC) based on the EC. To this end, an artificial neural network (ANN) was implemented for conventionally (CP) and non-thermally (NP) pasteurized milk. The findings indicated no significant difference (p > 0.05) between the experimental and predicted data for EC and MC. The MFR and EFS affected the actual EC. The raw milk samples had an EC of 0.468812–0.46913 S/m and MC of 87.3218–87.35941%, while these values in NP pasteurized milk were 0.457441–0.638224 S/m and 87.33986–87.40851%. With correlation coefficients (R) of 0.736478106–0.951840323 and mean square errors (MSE) of 0.005539–0.0064, the ANN accurately predicted the raw and pasteurized milk MC based on the EC using the sixth-order polynomial model and the EC based on the EFS and MFR using a quadratic model. The EC of pasteurized milk by NP was significantly (p < 0.05) lower than that of CP and raw dairy by 15.44% and 11.30%, respectively. The results show that the EFS and MFR might be used for the online assessment of milk’s physical attributes (e.g., EC), followed by using the assessed parameter to determine other properties (e.g., MC) by developing AI approaches based on optimized models. These observations showcase the innovative use of ANN-based AI to predict milk’s EC and MC accurately. Integrating such AI platforms into non-thermal food processing could eventually develop more sustainable food production and enhance food security and quality through process innovation and sustainable manufacturing, contributing to the industrial revolution and sustainable development goals. Full article
(This article belongs to the Special Issue Non-thermal Technologies in Food Science, 2nd Edition)
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Figure 1

Figure 1
<p>The feedforward neural network with multilayers: (<b>a</b>) prediction of electrical conductivity, (<b>b</b>) prediction of moisture content of pasteurized milk, (<b>c</b>) prediction of moisture content of raw milk. EFS: electric field strength, MFR: mass flow rate, EC: electric conductivity.</p>
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<p>ANN performance: (<b>a</b>) mean square error (MSE) and (<b>b</b>) correlation coefficients (R) for training, validation, test, and all while the target and the output are the experimental and predicted EC, respectively).</p>
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<p>The effect of EFS and MFR on EC predicted by ANNs: (<b>a</b>) 3D plot, (<b>b</b>) contour plot.</p>
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<p>Visual representation of ANN performance; (<b>a</b>): mean square error (MSE) of pasteurized milk; (<b>b</b>): correlation coefficients (R) of pasteurized milk; (<b>c</b>): MES of raw milk; (<b>d</b>): R of raw milk. The target is the experimental MC, and the output is the predicted MC.</p>
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<p>The predicted moisture content (MC) vs. electrical conductivity (EC) curves of pasteurized (<b>a</b>) and raw milk (<b>b</b>).</p>
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