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Applied Sciences
Volume 14
Issue 23
10.3390/app142311121
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Ex Vivo Fusion Confocal Microscopy of Liver Biopsies: Diagnostic Pattern Identification and Correlation with Conventional Microscopy

by
Sandra Lopez-Prades
1,2,3,
Carla Fuster-Anglada
1,2,3,4,5,
Rosana Millán
1,
Joana Ferrer-Fàbrega
2,4,5,6,
Octavi Bassegoda
2,3,5,7,
Loreto Boix
3,4,5,
Miriam Cuatrecasas
1,2,3,5 and
Alba Díaz
1,2,3,4,5,*
1
Pathology Department, Hospital Clinic Barcelona. C/Villarroel 170, 08036 Barcelona, Spain
2
Faculty of Medicine, University of Barcelona, C/Casanova 143, 08036 Barcelona, Spain
3
Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), C/Roselló 149, 08036 Barcelona, Spain
4
Barcelona Clínic Liver Cancer (BCLC) Group, IDIBAPS, C/Roselló 149, 08036 Barcelona, Spain
5
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBERehd), Av. Monforte de Lemos, 3-5, 28029 Madrid, Spain
6
Hepatobiliopancreatic Surgery and Liver and Pancreatic Transplantation Unit, Department of Surgery, Hospital Clinic Barcelona, C/Villarroel 170, 08036 Barcelona, Spain
7
Liver ICU, Liver Unit, Hospital Clínic Barcelona, C/Villarroel 170, 08036 Barcelona, Spain
*
Author to whom correspondence should be addressed.
Appl. Sci. 2024, 14(23), 11121; https://doi.org/10.3390/app142311121 (registering DOI)
Submission received: 26 October 2024 / Revised: 23 November 2024 / Accepted: 26 November 2024 / Published: 28 November 2024
Versions Notes

Abstract

Ex vivo Fusion Confocal Microscopy (eFuCM) is a promising new technique for real-time histological diagnosis, requiring minimal tissue preparation and avoiding tissue waste. This study aimed to evaluate the feasibility of eFuCM in identifying key liver biopsy lesions and patterns, and to assess the impact of eFuCM reading experience on diagnostic accuracy. Twenty-three fresh liver biopsies were analyzed using eFuCM to produce H&E-like digital images, which were reviewed by two pathologists and compared with a conventional H&E diagnosis. The liver architecture was clearly visible on the eFuCM images. Pathologist 1, with no prior eFuCM experience, achieved a substantial agreement with the H&E diagnosis (κ = 0.65), while Pathologist 2, with eFuCM experience, reached almost perfect agreement (κ = 0.88). However, lower agreement levels were found in the evaluation of inflammation. Importantly, tissue preparation for eFuCM did not compromise subsequent conventional histological processing. These findings suggest that eFuCM has great potential as a time- and material-saving tool in liver pathology, though its diagnostic accuracy improves with pathologist experience, indicating that there is a learning curve related to its use.
Keywords: ex vivo fusion confocal microscopy; liver pathology; minimal sample preparation; time-efficient diagnosis ex vivo fusion confocal microscopy; liver pathology; minimal sample preparation; time-efficient diagnosis

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MDPI and ACS Style

Lopez-Prades, S.; Fuster-Anglada, C.; Millán, R.; Ferrer-Fàbrega, J.; Bassegoda, O.; Boix, L.; Cuatrecasas, M.; Díaz, A. Ex Vivo Fusion Confocal Microscopy of Liver Biopsies: Diagnostic Pattern Identification and Correlation with Conventional Microscopy. Appl. Sci. 2024, 14, 11121. https://doi.org/10.3390/app142311121

AMA Style

Lopez-Prades S, Fuster-Anglada C, Millán R, Ferrer-Fàbrega J, Bassegoda O, Boix L, Cuatrecasas M, Díaz A. Ex Vivo Fusion Confocal Microscopy of Liver Biopsies: Diagnostic Pattern Identification and Correlation with Conventional Microscopy. Applied Sciences. 2024; 14(23):11121. https://doi.org/10.3390/app142311121

Chicago/Turabian Style

Lopez-Prades, Sandra, Carla Fuster-Anglada, Rosana Millán, Joana Ferrer-Fàbrega, Octavi Bassegoda, Loreto Boix, Miriam Cuatrecasas, and Alba Díaz. 2024. "Ex Vivo Fusion Confocal Microscopy of Liver Biopsies: Diagnostic Pattern Identification and Correlation with Conventional Microscopy" Applied Sciences 14, no. 23: 11121. https://doi.org/10.3390/app142311121

APA Style

Lopez-Prades, S., Fuster-Anglada, C., Millán, R., Ferrer-Fàbrega, J., Bassegoda, O., Boix, L., Cuatrecasas, M., & Díaz, A. (2024). Ex Vivo Fusion Confocal Microscopy of Liver Biopsies: Diagnostic Pattern Identification and Correlation with Conventional Microscopy. Applied Sciences, 14(23), 11121. https://doi.org/10.3390/app142311121

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