Diarylheptanoids from <i>Alnus japonica</i> Inhibit Papain-Like Protease of Severe Acute Respiratory Syndrome Coronavirus

Regular Articles

Diarylheptanoids from Alnus japonica Inhibit Papain-Like Protease of Severe Acute Respiratory Syndrome Coronavirus

School of Biological Science and Biotechnology, Chonnam National University">Ji-Young Park, Hyung Jae Jeong, Jang Hoon Kim, Young Min Kim, Su-Jin Park, Doman Kim, Ki Hun Park, Woo Song Lee, Young Bae Ryu

Author information

Ji-Young Park
Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)
School of Biological Science and Biotechnology, Chonnam National University
Hyung Jae Jeong
Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)
Jang Hoon Kim
Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)
Young Min Kim
Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)
Su-Jin Park
Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)
Doman Kim
School of Biological Science and Biotechnology, Chonnam National University
Ki Hun Park
Division of Applied Life Science (BK 21 Program, IALS), Graduate School of Gyeongsang National University
Woo Song Lee
Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)
Young Bae Ryu
Infection Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)

Keywords: diarylheptanoid, Alnus japonica, severe acute respiratory syndrome, papain-like protease, cysteine protease

JOURNAL FREE ACCESS
Supplementary material

2012 Volume 35 Issue 11 Pages 2036-2042

DOI https://doi.org/10.1248/bpb.b12-00623

Browse “Advance online publication” version

Details

Abstract

The papain-like protease (PL^pro), which controls replication of the severe acute respiratory syndrome coronavirus (SARS-CoV), has been identified as a potential drug target for the treatment of SARS. An intensive hunt for effective anti-SARS drugs has been undertaken by screening for natural product inhibitors that target SARS-CoV PL^pro. In this study, diarylheptanoids 1–9 were isolated from Alnus japonica, and the inhibitory activities of these compounds against PL^pro were determined. Of the isolated diarylheptanoids, hirsutenone (2) showed the most potent PL^pro inhibitory activity, with an inhibitory concentration (IC₅₀) value of 4.1 µM. Structure–activity analysis showed that catechol and α,β-unsaturated carbonyl moiety in the molecule were the key requirement for SARS-CoV cysteine protease inhibition.

Corresponding author

Register with J-STAGE for free!