Charles Martinet
- Mario
- (voice)
- …
Samantha Kelly
- Princess Peach
- (voice)
- …
Deanna Mustard
- Princess Daisy
- (voice)
Kazumi Totaka
- Yoshi
- (voice)
- …
Kenny James
- Bowser
- (voice)
Caety Sagoian
- Bowser Jr.
- (voice)
Sanae Uchida
- Boo
- (voice)
- (as Sanae Susaki)
Takashi Nagasako
- Donkey Kong
- (voice)
Katsumi Suzuki
- Diddy Kong
- (voice)
Atsushi Masaki
- Kamek
- (voice)
- (as Atsushi Masaka)
Nate Bihldorff
- Shy Guy
- (voice)
Tadd Morgan
- Lakitu
- (voice)
Tomo Adachi
- Mii
- (voice)
Yûko Kaida
- Mii
- (voice)
- (as Yuko Kaida)
Ayumi Nagao
- Mii
- (voice)
Hitomi Hirose
- Mii
- (voice)
- (as Hito Hirose)
Fumihiro Okabayashi
- Mii
- (voice)
- (as Humihiro Okabayasgi)
Takuya Satô
- Mii
- (voice)