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Invitrogen™ GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit (with competent cells)
Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.
Supplier: Invitrogen™ A21177
Alert:
For research use only. Not for use in diagnostic procedures.
Includes:
- CRISPR CD4 Nuclease Vector, linearized
- 10X Oligonucleotide Annealing Buffer
- DNase/RNase-free Water
- 5X Ligation Buffer
- T4 DNA Ligase
- U6 Forward Sequencing Primer
- Control Double-stranded Oligonucleotide
- OneShot™ TOP10 Chemically Competent Cells
Description
Simple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with a CD4 reporter. Competent cells included for added convenience and highest cloning efficiencies.GeneArt CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads CD4 magnetic beads. Transfection efficiency can also be tracked using anti-CD4 fluorescent antibodies. The linearized GeneArt CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. A version with competent cells is also available (Cat. No. A21177).
The GeneArt™ CRISPR Nuclease Vector system with competent cells offers
- Easy-to-design genome engineering system
- Affordable, ready-to-use cloning vectors
- Enrichment for hard to transfect or difficult to modify cell lines
Certifications
CoA
Compliance
CoA
Recommended Storage
Store in freezer (-5 to -30°C)
Specifications
Restriction Enzyme/MCS | |
Primer-probe | |
Kit | |
CRISPR-Cas9 | |
Crispr Nuclease Vector With CD4 Enrichment Kit | |
CD4 fusion | |
CD4 | |
Gene Expression, Cloning | |
3' overhangs |
• CRISPR CD4 Nuclease Vector, linearized • 10X Oligonucleotide Annealing Buffer • DNase/RNase-free Water • 5X Ligation Buffer • T4 DNA Ligase • U6 Forward Sequencing Primer • Control, double stranded oligonucleotide Store the above at -5 to -30°C. • OneShot™ TOP10 Chemically Competent Cells Store at -80°C. |
|
Liquid | |
Fast | |
GeneArt™ | |
U6, CMV | |
10 reactions | |
10 Reactions | |
10 Reactions | |
Oligos (DNA) |
For Research Use Only. Not for use in diagnostic procedures.