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Invitrogen™ GeneArt™ CRISPR Nuclease Vector with CD4 Enrichment Kit (with competent cells) DFS Item

Simple, ready-to-use, all-in-one expression vector system for CRISPR-based genome editing.

Supplier:  Invitrogen™ A21177

Catalog No. A21177


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Alert:

For research use only. Not for use in diagnostic procedures.


Includes:
  • CRISPR CD4 Nuclease Vector, linearized
  • 10X Oligonucleotide Annealing Buffer
  • DNase/RNase-free Water
  • 5X Ligation Buffer
  • T4 DNA Ligase
  • U6 Forward Sequencing Primer
  • Control Double-stranded Oligonucleotide
  • OneShot™ TOP10 Chemically Competent Cells

Description

Description

Simple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with a CD4 reporter. Competent cells included for added convenience and highest cloning efficiencies.

GeneArt CRISPR Nuclease Vector with CD4 Enrichment Kit is a vector system for expression of the functional components needed for CRISPR-Cas9 genome editing in mammalian cells with a CD4 reporter. The CD4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of Cas9 & CRISPR expressing cells using Dynabeads CD4 magnetic beads. Transfection efficiency can also be tracked using anti-CD4 fluorescent antibodies. The linearized GeneArt CRISPR nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crRNA representing a desired target into an expression cassette that allows sequence-specific targeting of the Cas9 nuclease. A version with competent cells is also available (Cat. No. A21177).


The GeneArt™ CRISPR Nuclease Vector system with competent cells offers
  • Easy-to-design genome engineering system
  • Affordable, ready-to-use cloning vectors
  • Enrichment for hard to transfect or difficult to modify cell lines
For research use only. Not for use in diagnostic procedures.

Certifications

CoA

Compliance

CoA

Recommended Storage

Store in freezer (-5 to -30°C)

Specifications

Specifications

Restriction Enzyme/MCS
Primer-probe
Kit
CRISPR-Cas9
Crispr Nuclease Vector With CD4 Enrichment Kit
CD4 fusion
CD4
Gene Expression, Cloning
3' overhangs
• CRISPR CD4 Nuclease Vector, linearized
• 10X Oligonucleotide Annealing Buffer
• DNase/RNase-free Water
• 5X Ligation Buffer
• T4 DNA Ligase
• U6 Forward Sequencing Primer
• Control, double stranded oligonucleotide
Store the above at -5 to -30°C.

• OneShot™ TOP10 Chemically Competent Cells
Store at -80°C.
Liquid
Fast
GeneArt™
U6, CMV
10 reactions
10 Reactions
10 Reactions
Oligos (DNA)
Videos
SDS
Documents

Documents

Promotions

Promotions

For Research Use Only. Not for use in diagnostic procedures.