In this episode, we explore the cutting-edge techniques revolutionizing fluorescence microscopy. Traditional methods are limited to detecting three to four targets due to the spectral overlap of fluorophores. But now, scientists are breaking these barriers with fluorescence lifetime multiplexing. This innovative approach uses differences in fluorescence lifetimes to distinguish between similar dyes, allowing for more complex imaging.Hear how researchers combined lifetime multiplexing with spectral detection to analyze multiple targets simultaneously. Discover the exciting advancements made by characterizing 18 synthetic fluorophores and developing unique self-labeling protein tags. These breakthroughs enable the imaging of up to eight targets in four spectral channels, providing unprecedented detail in cellular studies.Watch the full presentation here: https://microscopyfocus.com/live-cell-fluorescence-lifetime-multiplexing-using-organic-fluorophores/Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/