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7h2b

X-ray diffraction
1.6Å resolution

Function and Biology Details

Reactions catalysed:
[a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleosidein mRNA + S-adenosyl-L-homocysteine., a 5'-end (5'-triphosphoguanosine)-ribonucleoside in mRNA + S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleosidein mRNA + S-adenosyl-L-homocysteine.]
[a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA +S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside) in mRNA + S-adenosyl-L-homocysteine + H(+)., a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside in mRNA +S-adenosyl-L-methionine = a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleoside) in mRNA + S-adenosyl-L-homocysteine + H(+).]
[RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate., RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate.]
[Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaacan be either Arg or Lys and Yaa can be either Ser or Ala., Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaacan be either Arg or Lys and Yaa can be either Ser or Ala.]
[a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate+ phosphate + H(+)., a ribonucleoside 5'-triphosphate + H2O = a ribonucleoside 5'-diphosphate+ phosphate + H(+).]
[ATP + H2O = ADP + phosphate + H(+)., ATP + H2O = ADP + phosphate + H(+).]
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174292 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine protease subunit NS2B Chain: A
Molecule details ›
Chain: A
Length: 46 amino acids
Theoretical weight: 5.07 KDa
Source organism: Zika virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q32ZE1 (Residues: 1414-1457; Coverage: 1%)
Sequence domains: Flavivirus non-structural protein NS2B
Serine protease NS3 Chain: B
Molecule details ›
Chain: B
Length: 168 amino acids
Theoretical weight: 18.12 KDa
Source organism: Zika virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q32ZE1 (Residues: 1509-1675; Coverage: 5%)
Sequence domains: Peptidase S7, Flavivirus NS3 serine protease

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P4322
Unit cell:
a: 42.3Å b: 42.3Å c: 216.631Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.228 0.253
Expression system: Escherichia coli