Researchers must instead eliminate rRNA, most often using probes that target rRNAs for isolation and capture. While poly-A+ selection methods have not changed much in recent years, rRNA...

Aug 22, 2019 · In this study we describe the use of biotinylated oligos and streptavidin-coated paramagnetic beads for the efficient and specific depletion of trypanosomal rRNA. Our approach reduces the...

RiboMinus rRNA depletion technology is designed to enrich the whole spectrum of RNA transcripts by selectively depleting ribosomal RNA molecules (rRNA), regardless of their polyadenylation status or the presence of a 5'-cap structure.

Apr 6, 2022 · Current methods of high-throughput RNA sequencing of prokaryotes, including transcriptome analysis or ribosomal profiling, need deep sequencing to achieve sufficient numbers of effective reads...

Apr 21, 2020 · Here, we report the development of a "do-it-yourself" kit for rapid, cost-effective, and robust depletion of rRNA from total RNA. We present an algorithm for designing biotinylated oligonucleotides that will hybridize to the rRNAs from a target set of species.

Here, we report the development of a “do-it-yourself” kit for rapid, cost-effective, and robust depletion of rRNA from total RNA. We present an algorithm for designing biotinylated oligonucleotides that will hybridize to the rRNAs from a target set of species.

Here, we describe a quick, cost-effective method for depleting rRNAs using custom-designed oligos, using chickens as an example species for probe design.

Here, we describe Ribo-Pop (ribosomal RNA depletion for popular use), a simple workflow and antisense oligo design strategy that we demonstrate works over a wide input range and can be easily adapted to any organism with a sequenced genome.

The NEBNext ® rRNA Depletion Kit (Bacteria) employs the NEBNext RNase H-based RNA depletion workflow to deplete rRNA (5S, 16S, and 23S) from gram-positive and gram-negative organisms. View species tested to date.

The most common method of rRNA depletion is poly-A selection, which relies on the use of oligo (dT) primers attached to a solid support (e.g. magnetic beads) to isolate protein-coding polyadenylated RNA transcripts.

3 days ago · To maintain translational fidelity, cells degrade decoding-incompetent ribosomes through the 18S nonfunctional rRNA decay (NRD) pathway. Coria et al. demonstrate that the concerted actions of GCN2, RNF10, and RIOK3 lead to a block in translation initiation, ubiquitination of faulty ribosomes, and, ultimately, the degradation of nonfunctional 18S rRNA.

Dec 27, 2019 · We have developed a new RNase H-based ribosomal RNA depletion method for bacterial transcriptomics that is compatible with barcoded or fragmented RNA libraries.

As rRNAs are the most abundant RNA species in most samples, RNA depletion often refers to rRNA depletion, but other species may also be targeted if they obscure a biologically significant readout, as in the case of the depletion of globin mRNA from blood samples.

May 16, 2024 · To address these challenges, we developed r RNA R emoval by R NaseH (rRRR), a method to efficiently deplete rRNAs from a wide range of human, mouse, and rat RNA inputs and of varying qualities at a cost 10- to 20-fold cheaper than other approaches.

One for All. Universal rRNA Depletion for Any Organism. Highlights • Universal Depletion: Novel probefree technology depletes rRNA - from any organism. • Simplest Library Prep: Simultaneous ligation of both adapters reduces hands-on processing. • Automation Friendly: Streamlined protocol for increased scalability. Catalog Numbers: R3000 ...

Here, we present an optimized rRNA depletion protocol using RNase H and DNA oligonucleotides complementary to human rRNA transcripts. This protocol includes guidelines for DNA oligo preparation, RNA:DNA hybridization, RNase H cleavage and RNA cleanup, and benchmarking of rRNA depletion.

Jun 2, 2014 · In this study, we examined rRNA-depleted libraries from total RNA of fresh-frozen (FF) and FFPE samples sequenced by mRNA-Seq, Ribo-Zero-Seq and DSN-Seq and compared these results across methods and with conventional DNA microarrays.

To address these challenges, we developed r RNA R emoval by R NaseH (rRRR), a method to efficiently deplete rRNAs from a wide range of human, mouse, and rat RNA inputs and of varying qualities at a cost 10- to 20-fold cheaper than other approaches.

In this study we describe the use of biotinylated oligos and streptavidin-coated paramagnetic beads for the efficient and specific depletion of trypanosomal rRNA. Our approach reduces the levels of the most abundant rRNA transcripts to less than 5% with minimal off-target effects.

One such method involves RNase H-mediated depletion, where specific hybridization of RNase H to rRNA guides enzymatic degradation, resulting in rRNA removal. Additionally, the use of capture probes designed to specifically target rRNA sequences provides an …

Apr 21, 2020 · Here, we report an easily implemented, scalable, and broadly applicable do-it-yourself (DIY) rRNA depletion kit. Our kit relies on the physical depletion of rRNA from a complex RNA mixture using biotinylated oligonucleotides specific to 5S, 16S, and 23S rRNA.

2 days ago · (G) Northern blot displaying SL1 RNA and 5S rRNA abundance in control and ATTF-6 depletion strains. The relative abundance of SL1 RNA was normalized to that of 5S rRNA in each biological replicate. (H) Bar plots showing total levels of Type I and Type II piRNAs in control and ATTF-6 deletion samples. Data are presented as mean ± SD from two ...

1 day ago · Similarly, the knockdown of DDX21 in Huh7 cells resulted in the accumulation of 47/45S pre-rRNA and 30S pre-rRNA but reductions in the 21S and 18S-E pre-rRNA signals (Supplemental Fig. S6B). These ...

1 day ago · In panel B. nc886 levels normalized first to 18S rRNA and then to the average of all T samples (–ΔΔCt in the x-axis). C Northern and Western blot of indicated genes. nc886, vtRNA1-1, and 5S rRNA were probed sequentially on the same blot. 5S rRNA and β-actin: loading controls. All samples were harvested at 48 h after transfection of ...