%0 Journal Article %1 Ueha_1991_651 %A Uehara, A. %A Yasukohchi, M. %A Ogata, S. %A Imanaga, I. %D 1991 %J Pflugers Arch %K AMP-Dependent Acid Acid, Acids, Adenosine Algorithms, Alternative Amino Aniline Animal, Animals, Arachidonic Asparagine, Aspartic Bilayers, Binding Binding, Biological Biological, Biotransformation, Cadmium, Calcium Calcium, Carrier Cations, Cattle, Cell Cells, Channel Channels, Chimeric Chloride, Cloning, Cobalt, Competitive, Compounds, Conductivity, Conserved Cricetulus, Cultured, Cyclic Cysteine, Cytoplasm, Dogs, Dose-Response Drug, Electric Electrophysiology, Enzyme Expression, Extracellular Fibroblasts, Fura-2, Gating, Gene Hamsters, Heart Heart, Humans, Hypoxia, In Inhibitors, Intercellular Ion Junctions, Kidney Kinases, Kinetics, Knockout, Lanthanu, Ligands, Line, Lipid Lithium, Lung, Magnesium, Manganese, Membrane Membrane, Membranes, Mice, Models, Molecular Molecular, Motifs, Permeability, Potentials, Protein Proteins, Radioisotopes, Relationship, Sequence, Sites, Space, Splicing, Substitution, Transport, Triphosphate, Tubules, Ventricles, Vitro, %N 6 %P 651--653 %T Activation by intracellular calcium of a potassium channel in cardiac sarcoplasmic reticulum. %V 417 %X The effects of low (pCa 7.5 to 3) concentrations of intracellular calcium ion on a single potassium channel in the sarcoplasmic reticulum of canine heart ventricular muscle were investigated using a planar lipid bilayer technique. The low concentrations were obtained by mixing EGTA and calcium chloride. By varying the pCa of the cytoplasmic face between 3 to 7.5, two novel effects were observed. First, an increase in the intracellular Ca$^2+$ concentration produced an increase in the unit current amplitude of open states; the voltage-current relationship was ohmic at these concentrations. Second, an increase in the Ca$^2+$ concentration increased the open probability. Both these effects of Ca$^2+$ were dose-dependent, and were consistently observed in all channels tested. Thus, the SR potassium channel observed appears to belong to the class of Ca$^2+$-activated potassium channels.

@article{Ueha_1991_651, abstract = {The effects of low (pCa 7.5 to 3) concentrations of intracellular calcium ion on a single potassium channel in the sarcoplasmic reticulum of canine heart ventricular muscle were investigated using a planar lipid bilayer technique. The low concentrations were obtained by mixing EGTA and calcium chloride. By varying the pCa of the cytoplasmic face between 3 to 7.5, two novel effects were observed. First, an increase in the intracellular {C}a$^{2+}$ concentration produced an increase in the unit current amplitude of open states; the voltage-current relationship was ohmic at these concentrations. Second, an increase in the {C}a$^{2+}$ concentration increased the open probability. Both these effects of {C}a$^{2+}$ were dose-dependent, and were consistently observed in all channels tested. Thus, the SR potassium channel observed appears to belong to the class of {C}a$^{2+}$-activated potassium channels.}, added-at = {2009-06-03T11:20:58.000+0200}, author = {Uehara, A. and Yasukohchi, M. and Ogata, S. and Imanaga, I.}, biburl = {https://www.bibsonomy.org/bibtex/24f09975660750c3bfbb6f4f62937c2e6/hake}, description = {The whole bibliography file I use.}, interhash = {a0191a355a0a4a511a65f12dd5cb3cb6}, intrahash = {4f09975660750c3bfbb6f4f62937c2e6}, journal = {Pflugers Arch}, keywords = {AMP-Dependent Acid Acid, Acids, Adenosine Algorithms, Alternative Amino Aniline Animal, Animals, Arachidonic Asparagine, Aspartic Bilayers, Binding Binding, Biological Biological, Biotransformation, Cadmium, Calcium Calcium, Carrier Cations, Cattle, Cell Cells, Channel Channels, Chimeric Chloride, Cloning, Cobalt, Competitive, Compounds, Conductivity, Conserved Cricetulus, Cultured, Cyclic Cysteine, Cytoplasm, Dogs, Dose-Response Drug, Electric Electrophysiology, Enzyme Expression, Extracellular Fibroblasts, Fura-2, Gating, Gene Hamsters, Heart Heart, Humans, Hypoxia, In Inhibitors, Intercellular Ion Junctions, Kidney Kinases, Kinetics, Knockout, Lanthanu, Ligands, Line, Lipid Lithium, Lung, Magnesium, Manganese, Membrane Membrane, Membranes, Mice, Models, Molecular Molecular, Motifs, Permeability, Potentials, Protein Proteins, Radioisotopes, Relationship, Sequence, Sites, Space, Splicing, Substitution, Transport, Triphosphate, Tubules, Ventricles, Vitro,}, month = Feb, number = 6, pages = {651--653}, pmid = {2057327}, timestamp = {2009-06-03T11:21:35.000+0200}, title = {Activation by intracellular calcium of a potassium channel in cardiac sarcoplasmic reticulum.}, volume = 417, year = 1991 }