In the present study, extracts from the bark and wood of Juniperus excelsa ssp. polycarpos were obtained with acetone solvent. Chemical composition were analyzed and compared by gas chromatography-mass spectrometry (GC-MS). The results showed that the major components identified in the bark acetone extract as trimethylsilyl (TMS) derivatives were β-d-glucofuranose, 1,2,3,5,6-pentakis-O-(TMS) (19.97\%), followed by pimaric acid TMS (18.89\%), d-mannopyranose,1,2,3,4,6-pentakis-O-(TMS) (13.90\%), d-fructose,1,3,4,5,6-pentakis-O-(TMS) (12.37\%). The major components identified in the wood acetone extract as trimethylsilyl (TMS) derivatives were pimaric acid TMS (24.56\%), followed by α-d-glucopyranoside,1,3,4,6-tetrakis-O-(TMS)-β-d-fructofuranosyl 2,3,4,6-tetrakis-O-(TMS) (21.39\%), β-d-galactopyranose,1,2,3,4,6-pentakis-O-(TMS) (12.10\%), d-glucose,2,3,4,5,6-pentakis-O-(TMS) (9.97\%), trifluoromethyl-bis-(TMS) methyl ketone (9.32\%). One of the more important components identified both in the bark and wood extracts was pimaric acid TMS. Cedrol as the essential oil was found in the acetone wood extract (0.72\%).