Hydrodynamic focusing is used in automated cell-counting instruments to: Prevent coincidence passage through the detection aperture Increase the visibility of the cells passing through the aperture Allow for maximal light absorbance through the cells passing through the aperture Ensure adequate uptake of fluorescent staining by the cells

Q: Density gradient centrifugation is an inexpensive cell separation technique. Mention 2 limitations…

A: Density gradient centrifugation is a common technique for isolating and purifying biomolecules and…

Q: What are the main instrumentation components difference between Uv-visible and Fluorescence…

A: Spectroscopy :-  Spectroscopy is basically an association of electromagnetic radiation with atoms ,…

Q: Given: The used ocular objective while taking this image, has magnifying power of 6x The used…

A: Given : Stage micrometer used has graduation = 0.01mm Ten graduation on ocular micrometer coincides…

Q: This is integral to obtaining the experimental data that lead to the development of this model…

A: Introduction ODE MODEL:- Ordinary differential equations or ODE models are used to model biological…

Q: What factors must be addressed to properly acquire quantitative fluorescence microscopy images? What…

A: Quantitative fluorescence microscopy is a type of microscopy used in biological studies in which the…

Q: An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and…

A: Introduction: Acid-fast staining is a type of differential staining technique that was first…

Q: Which of the following is not required for a UV-vis absorption spectrophotometer? Group of answer…

A: UV-Vis Spectroscopy is also known as Spectrophotometry and is a quantitative technique to determine…

Q: Both light and electron microscopy are commonly used to visualize cells, cell structures, and the…

A: The cell is the basic structural and functional unit of the body. It is very important to determine…

Q: Draw and label 3 cell types at low and high power. State the magnification for each one

A: Microscopes enable us to magnify specimens. In simple terms , magnification is an increase in size…

Q: During studying a thick heavily stained specimen under the microscope, it is better to light…

A: Microscopy is the technique of visualisation of microscopic specimens by using a microscope after…

Q: What is the best wavelength to study light scattering with bacteria?     260 nm     260-280 nm…

A: 260-280 nm The best wavelength to study light scattering with bacteria depends on the specific…

Q: What stain would you use to differentiate between bean and ant cells? Explain and include your…

A: Staining is a procedure that involves the application of colored chemicals called dyes to specimens.…

Q: Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture…

A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…

Q: e fluorescent label in this image is he cell shapes are imaged with The microscopy used for…

A: In the image given some kind of cells are present which are glowing.  Some cellular processes are…

Q: Staining of cells is often performed to enhance images acquired by brightfield microscopy.…

A: Microorganisms or microbes are small living organisms that can be observed with the aid of…

Q: You may want to use this resource for this problem. If you do, submit the output along with your…

A: The confocal microscope is an advanced imaging technique that allows for high-resolution and…

Q: staining

A: Staining-  It is used to enhance contrast in samples, generally at the microscopic level. In other…

Q: Explicitly discuss the importance of staining in cytology

A: Stains are dyes which impart different colours to different cellular structure and chemicals in the…

Q: Explain the principle of the isoelectric focusing electrophoresis method and what it is used for.

A: Proteins are defined as the biological molecule that are produced by the building blocks amino…

Q: Briefly describe how magnetic bead microrheometry is used to measure the mechanical properties of…

A: A magnetic bead microrheometry  has been designed which allows the generation of forces up to 4.5…

Q: What is the main advantage of fluorescence polarization? Sensitivity of fluorescent probes Low cost…

A: FP is an analytical technique which provides information on orientation and mobility of…

Q: Stains are used because O they bind evenly to all str they reduce contrast and make it easier to see…

A: Staining is a procedure in microscopy where a specimen to be viewed under the microscope is…

Q: The significance of using immersion oll when using 100X objective lens is: It will increase the…

A: Answer.  Microscopy is a technique for making very small things visible to the unaided eye. An…

Q: Given: The used ocular objective while taking this image, has magnifying power of 6x The used…

A: Magnification on a microscope denotes the amount or degree of visual enlargement of an observed…

Q: Your first task in the lab is to learn how to culture these cells and begin expanding the culture…

A: Answer:  Yes, the cell culture media need to be changed more often than the other. The open culture…

Q: Describe a technique that will allow you to separate cells from media

A: ANSWER) There are various techniques which are used to separate the cells from the medium. Some of…

Q: In light microscopy, when magnification is increased, the lens focuses closer to the sample.  This…

A: Microscope helps to observe the organisms or objects that cannot be seen by our naked eye because…

Q: The cell is usually placed after the monochromator, except in the case of using a multichannel…

A: A multi-channel analyzer is an instrument used in laboratory and field applications so to analyze an…

Q: Put the following steps for running a gel in order. first Remove comb and put gel int v Choose ]…

A: Agarose gel electrophoresis is the technique that involves the separation of substances by providing…

Q: In this problem, you will design a filter system for a flow cytometer. The four fluorescent dyes are…

A: The order in which the filters are arranged with respect to the unfiltered signal is determined by…

Q: You wish to centrifuge and pellet yeast cells using a centrifuge. The protocol says that you need to…

A: Centrifugation is the process by which particles in a solution are separated based on the density…

Q: Cellular stains are commonly used in bacteriology. Which of the following is NOT a reason to stain a…

A: Introduction :- Cell staining is a method that can be applied to improve microscopic views of cells…

Q: Compare and contrast the operation of Optical microscopy and TEM in terms of Abberations

A: Microscopes are widely employed to produce a magnified view of the materials. Magnification refers…

Q: Explain the difference between a transmission electronmicrograph (TEM) and a scanning electron…

A: A microscope is a laboratory instrument that is used to examine objects that are too small for the…

Q: Discuss the steps from dissection of tissue to producing a section on a slide ready for staining, as…

A: Human tissue has long been recognized for its extraordinary value in medical research. These cells…

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique to identify bacterial stain gram-positive or gram-negative. It can be…

Q: Which one of these techniques does not distinguish between live and dead cells? Group of answer…

A: Hi, Thanks For Your Question.  Answer : Correct Option Fluorescence Staining.

Q: Which of the following is an advantage of confocal microscopy over conventional fluorescence…

A: One significant advantage of confocal microscopy compared to conventional fluorescence microscopy is…