5 Egypt. J. Agronematol., Vol. 17, No.1, PP. 51-64 (2018) Description and Identification of Four Species of Plant-parasitic Nematodes Associated with Forage Legumes * * Mahfouz M. M. Abd-Elgawad ; Mohamed F.M. Eissa ; Abd-Elmoneim Y. ** El-Gindi ; Grover C. Smart *** **** ; and Ahmed El-bahrawy . * Plant Pathology Department, National Research Centre. ** Department of Agricultural Zoology and Nematology, Faculty of Agriculture, University of Cairo, Giza, Egypt. *** Department of Entomology and Nematology, IFAS, University of Florida, USA. **** Institute for Sustainable Plant Protection, National Council of Research, Bari, Italy. Abstract Four species of plant parasitic nematodes were present in soil samples planted with forage legumes at Alachua County, Florida, USA. The detected species Belonolaimus longicaudatus, Criconemella ornate, Hoplolaimus galeatus, and Paratrichodorus minor were described in the present study. They belong to orders Rhabditida (Belonolaimus longicaudatus, Criconemella ornate, and Hoplolaimus galeatus) and Triplonchida (Paratrichodorus minor) and to taxonomical families Dolichodoridae (Belonolaimus longicaudatus), Hoplolaimidae (Hoplolaimus galeatus) Criconematidae (Criconemella ornate), and Trichodoridae (Paratrichodorus minor). The identification of the present specimens was based on the classical taxonomy, following morphological and morphometrical characters in the species specific identification keys. Keywords: Belonolaimus longicaudatus, Criconemella ornate, Hoplolaimus galeatus, Paratrichodorus minor, morphology, species description. Introduction As is widely and rightly held nematodes (round worms) are diversified multicellular animals comprising free living to plant, animal and even humanparasitic species. They represent a group of the most numerous metazoans in soil and aquatic sediments. From an environmental point of view, nematodes are part of nearly all ecosystems in their roles as bacterivores, herbivores, parasites of animals and plants, and consumers of dissolved as well as particulate organic matter (Mekete et al., 2012). Among them, plant-parasitic nematodes (PPNs) form prominent and distinguished group. These latter are mostly hidden enemy of the growers as the nematodes are generally subterranean in habitats and farmers are 52 Mahfouz M. M. Abd-Elgawad, et al.,…. unaware of losses caused by them. A great loss to crops has been reported in quantitative, qualitative and monetary terms. Considering their impact on crops, Abd-Elgawad and Askary (2015) reported an average worldwide crop loss of 12.6% which equaled $215.77 billion due to these nematodes for only the top 20 life-sustaining crops based on the 2010-2013 production figures and prices. Moreover, 14.45% or $142.47 billion was an average annual yield loss in the subsequent group of food or export crops. These figures are staggering, and the authentic figure, when more crops are counted, certainly exceeds such estimations. Nevertheless, it is noteworthy that PPNs are among the least studied, with close to only 26,000 (estimated < 3%) species described to date (Hugot et al., 2001; Mekete et al., 2012). Accuracy of identification is, therefore, fundamental to our understanding and communication of the ecological role of any organism. It goes without saying that PPN identification has its strength in agricultural applications because of its economic participation in nematode management implications. Consequently, PPN species delimitation methods in the context of agricultural and health-related applications are more refined at the species and below species level than methods employed in nematode biodiversity studies. Yet, both groups of studies help in better understanding of nematology and its interaction with relevant disciplines. In fact, specimen identification are imperative not only for choosing adequate management control strategies for PPNs but also for avoiding spreading of such exotic nematodes in quarantine materials. Yet, PPNs are one of the hardest groups to be identified due to their microscopic sizes as well as the difficulties in observing key diagnostic characters/features under conventional light microscope (Carneiro et al., 2017). On the other hand, legumes represent some of the best quality forages for livestock since they are palatable, help maintain proper functioning of the ruminant digestive processes and stimulate high production of both meat and milk. Since they possess an excellent attribute of biological nitrogen fixation in association with rhizobia, which helps in sustaining soil fertility, legumes are used as green manure by ploughing them into the soil. Considering their impact on crops, PPNs associated with common forage legumes were studied for morphometric identification in this work. Materials and Methods Soil of three experimental areas with established stands of fifteen adapted clover cultivars were sampled for PPNs. The experimental areas, properties of the University of Florida, were located in Alachua County near Gainesville, Florida, USA at altitude of 30 degrees north and a longitude of 82 degrees west. The three experiments, set up initially to test forage cultivars, had been established in a randomized block design with clover cultivars and controls as treatments. The sampled clover cultivars comprised Trifolium repens (white clover) cvs 'FL-XP1', Egypt. J. Agronematol., Vol. 17, No. 1, (2018) Description and Identification of Four Species of Plant-parasitic Nematodes Associated …… 53 'Regal', 'FL-XP2', 'LA-SI', and 'Tillman'; T. pretense (red clover) cv. 'Kenstar'; T. vesiculosum (arrowleaf clover) cvs 'Amclo', 'REPS-5' and 'Yuchi'; T. incarnatum (crimson clover) 'Dixie'; T. subterraneum (subterranean clover) cvs 'Mt. Barker' and 'Woogenellup'; T. alexandrinum (Egyptian clover) cv. 'Bigbee'; Medicago sativa (alfalfa) cv. 'FL-77'; and Melilotus alba var annua (sweet clover) cv. 'Hubam'. Phytonematodes associated with the forage legumes of these experiments were reported to the generic level (Abd-Elgawad et al., 2017). About five 2.5-cm diameter cores were taken randomly within the root zone (upper 15-20 cm) from each plot and then mixed thoroughly to form one sample representing the plot. Each sample was placed in a plastic bag, stored in an ice chest, and transported to the nematology laboratory. Samples not processed immediately were stored at 10 ± 1 0 3 C until processed. Nematodes were extracted from 100 cm soil from each sample using a centrifugal-flotation technique (Caveness and Jensen, 1955), placed in vials, and stored in a refrigerator at 4C for no more than three days until identified to genera and counted. Thereafter, some of the nematodes extracted from the study (Abd-Elgawad et al., 2017) were taken and processed for identification to the species level as originally described by Goodey (1963). Three percent formalin was used for making temporary mounts of nematodes. One percent water agar was ® used (for en face mounts) and Zut slide-ringing compound was used to seal the slides. Drawings were made with the camera lucida technique equipped to the microscope. All measurements except ratios are expressed in μm as means ± standard deviation. Key references of Rau (1963), Luc and Raski (1981), Jairajpuri and Baqri (1973), and Decraemer (1980) were consulted to identify species of Belonolaimus, Criconernella, Hoplolaimus, and Paratrichodorus. A magnification of 1000 x was used to identify all nematodes. Throughout these identifications, we adopted the systematic scheme of De Ley and Blaxter (2002) for the higher classification which has been updated where appropriate by Decraemer and Hunt (2013) to reflect new taxa proposals. Results and Discussions Species of four genera of plant-parasitic nematodes associated with all the above-mentioned clover cultivars were identified as Belonolaimus longicaudatus, Criconemella ornate, Hoplolaimus galeatus, and Paratrichodorus minor (Tables 1-4; Figs. 1-4). Measurements and drawings of nematode species are presented in Tables 1 through 4 and Figures 1 through 4; respectively. Golden (1971) classified the genera and higher categories of the order Tylenchida in which all of these nematodes are placed except Paratrichodorus minor which is in the order Dorylamida and published by Siddiqi (1973). Nevertheless, family Trichodoridae is no longer part of Dorylaimida and Tylenchida doesn't exist (now it is Rhabditida). In fact, recent molecular phylogenetic analyses recognize 12 clades within the Nematoda, with plant-parasitic taxa located in the basic clade I (Trichodoridae) and clade II (Longidoridae) and in the more advanced clade 12 with the Egypt. J. Agronematol., Vol. 17, No. 1, (2018) 54 Mahfouz M. M. Abd-Elgawad, et al.,…. Tylenchomorpha (Holterman et al., 2006; Decraemer and Hunt, 2013). Accordingly, the species identified herein belong to orders Rhabditida (Belonolaimus longicaudatus, Criconemella ornate, and Hoplolaimus galeatus) and Triplonchida (Paratrichodorus minor) and to taxonomical families Dolichodoridae (Belonolaimus longicaudatus), Hoplolaimidae (Hoplolaimus galeatus) Criconematidae (Criconemella ornate), and Trichodoridae (Paratrichodorus minor). The morphometric identification carried out herein neither negates the need for molecular tools for its documentation nor declines the presence of other PPN species associated with such forages (Abd-Elgawad et al., 2018). In fact, PPNs have been receiving sufficient attention regarding their taxonomy and evolution and therefore they are currently undergoing continuous modifications in their classification, phylogeny and taxonomy (e.g. Sun et al., 2014; Guesmi-Mzoughi et al., 2016). Belonolaimus longicaudatus (Table1; Fig.1) was identified using the dichotomous key of Rau (1963). He stated that this species is different from B. euthychilus, B. gracilis, and B. maritimus to which it is related in ratio of stylet length to tail length. Sixty to 100% of B. longicaudatus populations have stylets shorter than tails while the opposite is true with the other species. B. longicaudatus differs from B. nortoni in that B. longicaudatus has a relatively longer stylet and lips of vulva are not protruding. Table (1): Measurements and ratios of females and males of Belonolaimus longicaudatus associated with forage legumes. Body regions and organs Body length Dimensions (μm) and ratios Females (n= 10) Males (n= 10) Minimum Maximum Average±S.D. Minimum Maximum Average±S.D. 2085 2871 2395 ± 121 1987 2193 2031 ± 136 Body diameter 36 44 41 38 43 41 Tail length 120 172 150 ± 14.8 110 169 141 ±16 Anal body diameter 32 38 34 29 34 31 Stylet length 118 129 123 ± 11.3 112 119 115 ± 11 Esophagus length 268 290 276 314 335 325 Distance from head to vulva 1028 1520 1278 -- - - - - - 39 47 43 Spicule length A ratio 62 67 - 61 65 - B ratio 7.8 8.3 - 6.6 7.3 - C ratio 14 19 - 13 15 - Ć ratio 3.8 4.5 - 3.8 3.9 - V% 49% 55% — - - - Egypt. J. Agronematol., Vol. 17, No. 1, (2018) Description and Identification of Four Species of Plant-parasitic Nematodes Associated …… 55 The image cannot be displayed. Your computer may not hav e enough memory to open the image, or the image may hav e been corrupted. Restart your computer, and then open the file again. If the red x still appears, y ou may hav e to delete the image and then insert it again. Fig. (1): Belonolaimus longicaudatus (Rau, 1958). (A) Anterior body region, (B) Head region, (C) Vulval region, (D) Female tail, E - Male tail. Criconemella ornate (Table 2; Fig. 2) is related to C. curvatum but differs from the latter by the absence of labial plates and by the pointed outline of the anterior vulvar flap (Raski, 1952). Additional important features of C. ornate is the presence of the sub-lateral lobes and the relatively few annules, i.e., 87 to 92 annules (Raski, 1958). Egypt. J. Agronematol., Vol. 17, No. 1, (2018) 56 Mahfouz M. M. Abd-Elgawad, et al.,…. Table (2): Measurements and ratios of females of Criconemella ornate associated with forage legumes. Dimensions (μm) and ratios(n= 13) Body regions and organs Body length Body diameter Tail length Anal body diameter Stylet length Esophagus length Distance from head to vulva Spicule length A ratio B ratio C ratio Ć ratio V% Minimum Maximum Average ± S.D. 340 28 14 13 44 85 310 9 3 16 1.1 86% 460 46 27 24 61 127 395 -16 4 27 1.1 96% 404 ± 34 37 21 ± 5.4 19 52 ± 4.5 103 366 -----91% Jairajpuri and Baqri (1973) presented a key for Hoplolaimus spp. They differentiated between H. Tylenchiformis, having three annules in the lip region and female tails usually bluntly rounded, and H. galeatus (Table 3; Fig. 3), having four or more annules and female tails rounded. Also, they used the following characteristics of H. galeatus to differentiate it from other Hoplolaimus spp.: four incisures on the body, excretory pore located below the hemizonid, one phasmid in anterior part of the body and one phasmid in the posterior part of it, and spicules 40 - 50 μm long. Table (3): Measurements and ratios of females and males of Hoplolaimus galeatus associated with forage legumes. Dimensions (μm) and ratios Females (n= 10) Body regions and organs Minimum Maximum Body length Body diameter Tail length Anal body diameter Stylet length Esophagus length Distance from head to vulva Spicule length A ratio B ratio C ratio Ć ratio V% 1283 40 15 31 48 178 736 -25.8 6.7 38.4 0.5 52% Egypt. J. Agronematol., Vol. 17, No. 1, (2018) 1643 55 37 39 60 220 853 -37.8 8.4 97.6 1.0 58% Average ± S.D. 1464 ± 119 47 26 ± 8 35 5.2 ± 4.6 200 807 ------- Males (n= 4) Minimum Maximum 983 35 20 25 42 165 -51 28.1 6 37.3 0.8 -- 1195 42 32 33 50 190 -59 28.5 6.3 49.2 1.0 -- Average ± S.D. 1072 ± 99 39 26 ± 5 28 45 ± 3.6 180 -55 ------ Description and Identification of Four Species of Plant-parasitic Nematodes Associated …… 57 Fig. (2): Criconemella ornate (Raaki, 1958, Luc & Raski, 1981). (A) Adult female, (B) Head en face view, (C) Female tail, (D) Anterior body region. Egypt. J. Agronematol., Vol. 17, No. 1, (2018) 58 Mahfouz M. M. Abd-Elgawad, et al.,…. Fig. (3): Hoplolabnus galeatus (Cobb, 1913, Thorne, 1935). (A) Vulval region, (B) Female tail, (C) Anterior body region, (D) Male, head region, (E) Male, tail region. Egypt. J. Agronematol., Vol. 17, No. 1, (2018) 59 Description and Identification of Four Species of Plant-parasitic Nematodes Associated …… After Siddiqi's division (1973) of the genus Trichodorus into Paratrichodorus and Trichodorus, there had been considerable confusion in the literature because some authors apparently had not recognized Paratrichodorus spp. as a valid genus, whereas others had (Perry and Rhoades, 1982). Now, both genera are well established (e.g., Decraemer and Hunt, 2013). In our specimens (Table 4; Fig. 4) the two characters used for separating the genus, i.e. male with bursa and female with weak and indistinct sclerotization between the vulva and vagina were recognizable and it keys to P. christiei. Yet, our specimens agreed with the synonomy of P. christiei and P. minor since the lengths of the onchiostyle and spicules of our specimens overlap those of both species (Loof, 1975; Decraemer, 1980). Table (4): Measurements and ratios of females and males of Paratrichodorus minor associated with forage legumes. Dimensions (μm) and ratios Females (n= 10) Body regions and organs Average± Minimum Maximum S.D. Males (n= 4) Minimum Maximum Average ± S.D. Body length 462 735 610 ±117 589 692 633 ± 4 Body diameter 30 49 35 34 38 37 Tail length -- -- -- 19 23 20 ± 2 Anal body diameter -- -- -- 9 12 10 Stylet length 30 48 41 ± 8 32 40 36 ± 4 Esophagus length 96 122 106 85 90 87 Distance from head to vulva 240 370 310 -- -- -- Spicule length -- -- -- 58 66 61 A ratio 15 15.4 15.2 17.3 18.2 -- B ratio 4.8 6 5.4 6.9 7.7 -- C ratio -- -- -- 30 31 -- Ć ratio -- -- -- 1.9 2.1 -- 50% 52% 51% -- -- = V% Egypt. J. Agronematol., Vol. 17, No. 1, (2018) 60 Mahfouz M. M. Abd-Elgawad, et al.,…. Fig. (4) Paratrichodorus minor (Allen,1957, Siddiqi, 1973). (A) Adult female, (B) Female, anterior body region, (C) Male tail, (D) Vulval region, E- Female tail. Egypt. J. Agronematol., Vol. 17, No. 1, (2018) Description and Identification of Four Species of Plant-parasitic Nematodes Associated …… 61 Acknowledgement This study was supported in part by the NRC In-house project entitled 'Pesticide alternatives against soilborne pathogens attacking legume cultivation in Egypt'. References Abd-Elgawad M.M.M., Askary T.H. (2015). Impact of phytonematodes on agriculture economy. In: Biocontrol Agents of Phytonematodes. Askary, T.H., and Martinelli, P.R.P. (eds), pp. 3-49. CAB International, Wallingford, UK. Abd-Elgawad, M.M.M., Eissa, M.F.M., El-Gindi A.Y., and Smart, G.C. (2017) Plant-parasitic nematodes associated with forage legumes planted in fallow and into living grass sod. Egyptian Journal of Agronematology 16(1): 39-49. Abd-Elgawad, M.M.M., Eissa, M.F.M., El-Gindi A.Y., Smart, G.C. and Elbahrawy, A. (2018). Identification of five phytonematode species associated with forage legumes. Egyptian Journal of Agronematology 17(1): In press. 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Abd-Elgawad, et al.,….‬‬ ‫‪64‬‬ ‫وﺻﻒ وﺗﻌﺮﻳﻒ أرﺑﻌﺔ أﻧﻮاع ﻣﻦ اﻟﻨﻴﻤﺎﺗﻮدا اﻟﻤﺘﻄﻔﻠﺔ ﻧﺒﺎﺗﻴﺎ اﻟﻤﺮﺗﺒﻄﺔ ﺑﺎﻟﺒﻘﻮﻟﻴﺎت اﻟﻌﻠﻔﻴﺔ‬ ‫ﻣﺤﻔﻮظ ﻣﺤﻤﺪ ﻣﺼﻄﻔﻰ ﻋﺒﺪ اﻟﺠﻮاد*‪ ،‬ﻣﺤﻤﺪ ﻓﻬﻤﻲ ﻣﺤﻤﺪ ﻋﻴﺴﻲ*‪ ،‬ﻋﺒﺪ اﻟﻤﻨﻌﻢ ﻳﺎﺳﻴﻦ اﻟﺠﻨﺪي**‪،‬‬ ‫ﺟﺮوﻓﺮ ﺳﻤﺎرت***‪ ،‬وأﺣﻤﺪ اﻟﺒﺤﺮاوي‬ ‫*‬ ‫****‬ ‫ﻗﺴﻢ أﻣﺮاض اﻟﻨﺒﺎت‪ ،‬اﻟﻤﺮﻛﺰ اﻟﻘﻮﻣﻲ ﻟﻠﺒﺤﻮث‪ ،‬اﻟﺪﻗﻲ ‪ ،١٢٦٢٢‬اﻟﻘﺎﻫﺮة‪ ،‬ﻣﺼﺮ‪.‬‬ ‫** ﻗﺴﻢ اﻟﺤﻴﻮان واﻟﻨﻴﻤﺎﺗﻮﻟﻮﺟﻴﺎ اﻟﺰراﻋﻴﺔ‪ ،‬ﻛﻠﻴﺔ اﻟﺰراﻋﺔ‪ ،‬ﺟﺎﻣﻌﺔ اﻟﻘﺎﻫﺮة‪ ،‬اﻟﻘﺎﻫﺮة‪ ،‬ﻣﺼﺮ‪.‬‬ ‫*** ﻗﺴﻢ اﻟﺤﺸﺮات واﻟﻨﻴﻤﺎﺗﻮﻟﻮﺟﻲ‪ ،‬ﻣﻌﻬﺪ ﻋﻠﻮم اﻟﻐﺬاء واﻟﺰراﻋﺔ‪ ،‬ﺟﺎﻣﻌﺔ ﻓﻠﻮرﻳﺪا‪ ،‬اﻟﻮﻻﻳﺎت اﻟﻤﺘﺤﺪة اﻷﻣﺮﻳﻜﻴﺔ‪.‬‬ ‫**** ﻣﻌﻬﺪ اﻟﻮﻗﺎﻳﺔ اﻟﻤﺴﺘﺪاﻣﺔ ﻟﻠﻨﺒﺎت‪ ،‬اﻟﻤﺠﻠﺲ اﻟﻘﻮﻣﻲ ﻟﻠﺒﺤﻮث‪ ،‬ﻣﺪﻳﻨﺔ ﺑﺎري‪ ،‬إﻳﻄﺎﻟﻴﺎ‪.‬‬ ‫اﻟﻤﻠﺨﺺ اﻟﻌﺮﺑﻲ‬ ‫ﺗﻢ وﺻﻒ وﺗﻌﺮﻳﻒ أرﺑﻌﺔ أﻧﻮاع ﻣﻦ اﻟﻨﻴﻤﺎﺗﻮدا اﻟﻤﺘﻄﻔﻠﺔ ﻧﺒﺎﺗﻴﺎ اﻟﻤﺮﺗﺒﻄﺔ ﺑﺎﻟﺒﻘﻮﻟﻴﺎت اﻟﻌﻠﻔﻴﺔ ﻓﻲ ﻣﻘﺎﻃﻌﺔ‬ ‫اﻻﺷﻮا ﻓﻲ وﻻﻳﺔ ﻓﻠﻮرﻳﺪا ﺑﺎﻟﻮﻻﻳﺎت اﻟﻤﺘﺤﺪة اﻷﻣﺮﻳﻜﻴﺔ‪ .‬اﻷﻧﻮاع اﻟﻤﻜﺘﺸﻔﺔ ﻓﻲ ﻫﺬﻩ اﻟﺪراﺳﺔ ﻫﻲ‬ ‫ﺑﻴﻠﻮﻧﻮﻻﻳﻤﻮس ﻟﻮﻧﺠﻴﻜﻮداﺗﻮس‪ ،‬ﻛﺮﻳﻜﻮﻧﻴﻤﻴﻼ أورﻧﺎت‪ ،‬ﻫﻮﺑﻠﻮﻻﻳﻤﻮس ﺟﺎﻟﻴﺎﺗﻮس‪ ،‬وﺑﺎراﺗﺮﻳﻜﻮدوروس‬ ‫ﻣﻴﻨﻮر‪ ،‬وﻫﻲ ﺗﻨﺘﻤﻲ إﻟﻲ اﻟﺮﺗﺐ رﺑﺪﻳﺘﻴﺪا )ﺑﻴﻠﻮﻧﻮﻻﻳﻤﻮس ﻟﻮﻧﺠﻴﻜﻮداﺗﻮس‪ ،‬ﻛﺮﻳﻜﻮﻧﻴﻤﻴﻼ اورﻧﺎت‪،‬‬ ‫وﻫﻮﺑﻠﻮﻟﻴﻤﻮس ﺟﺎﻟﻴﺎﺗﻮس( وﺗﺮﻳﺒﻠﻮﻧﺸﻴﺪا )ﺑﺎراﺗﺮاﻳﻜﻮدوروس ﻣﻴﻨﻮر( واﻟﻌﺎﺋﻼت اﻟﺘﺼﻨﻴﻔﻴﺔ‬ ‫دوﻟﻴﻜﻮدورﻳﺪاي )ﺑﻴﻠﻮﻧﻮﻻﻳﻤﻮس ﻟﻮﻧﺠﻴﻜﻮداﺗﻮس(‪ ،‬ﻫﻮﺑﻮﻟﻮﻻﻳﻤﻴﺪاي )ﻫﻮﺑﻠﻮﻻﻳﻤﻮس ﺟﺎﻟﻴﺘﻮس(‬ ‫ﻛﺮﻳﻜﻮﻧﻤﺎﺗﻴﺪا )ﻛﺮﻳﻜﻮﻧﻴﻤﻴﻼ اورﻧﺎت(‪ ،‬وﺗﺮﻳﻜﻮدورﻳﺪاي )ﺑﺎراﺗﺮﻳﻜﻮدوروس ﻣﻴﻨﻮر(‪ .‬اﺳﺘﻨﺪ اﻟﺘﻌﺮﻳﻒ‬ ‫اﻟﺤﺎﻟﻲ ﻟﻬﺬﻩ اﻷﻧﻮاع إﻟﻰ اﻟﺘﺼﻨﻴﻒ اﻟﻜﻼﺳﻴﻜﻲ ﺑﺎﺳﺘﺨﺪام ﻣﻔﺎﺗﻴﺢ ﺗﺤﺪﻳﺪ اﻷﻧﻮاع اﻟﻤﺤﺪدة ﺑﻨﺎء ﻋﻠﻰ‬ ‫اﻟﺼﻔﺎت واﻟﻘﻴﺎﺳﺎت اﻟﻤﻮرﻓﻮﻟﻮﺟﻴﺔ )اﻟﺨﺎﺻﺔ ﺑﺎﻟﺸﻜﻞ اﻟﻈﺎﻫﺮي(‪.‬‬ ‫اﻟﻜﻠﻤﺎت اﻟﺪاﻟﺔ‪ :‬ﺑﻴﻠﻮﻧﻮﻻﻳﻤﻮس ﻟﻮﻧﺠﻴﻜﻮداﺗﻮس‪ -‬ﻛﺮﻳﻜﻮﻧﻴﻤﻴﻼ اورﻧﺎت‪ -‬ﻫﻮﺑﻠﻮﻻﻳﻤﻮس ﺟﺎﻟﻴﺎﺗﻮس‪-‬‬ ‫ﺑﺎراﺗﺮﻳﻜﻮدوروس ﻣﻴﻨﻮر‪ -‬اﻟﺸﻜﻞ اﻟﻈﺎﻫﺮي ‪ -‬وﺻﻒ اﻷﻧﻮاع‪.‬‬ ‫)‪Egypt. J. Agronematol., Vol. 17, No. 1, (2018‬‬