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2003, Molecular and Biochemical Parasitology
Molecular & cellular proteomics : MCP
Proteomics analysis of the excretory/secretory component of the blood-feeding stage of the hookworm, Ancylostoma caninum2009 •
International Journal for Parasitology
Identification of a DAF-7 ortholog from the hookworm Ancylostoma caninum2005 •
Journal of nematology
Identification of a secreted fatty acid and retinol-binding protein (Hp-FAR-1) from Heligmosomoides polygyrus2009 •
Hp-FAR-1 is a major, secreted antigen of the parasitic nematode Heligmosomoides polygyrus, a laboratory mouse model frequently used to study the cellular mechanisms of chronic helminth infections. The DNA encoding Hp-FAR-1 was recovered by screening a fourth larval (L₄) H. polygyrus cDNA expression library using antibodies raised against L₄ stage excretory/secretory (E/S) proteins. Predictions of secondary structure based on the Hp-FAR-1 amino acid sequence indicated that an alpha-helix predominates in Hp-FAR-1, possibly with some coiled-coil conformation, with no beta-structure. Fluorescence-based ligand binding analysis confirmed that the recombinant Hp-FAR-1 (rHp-FAR-1) binds the fluorescent fatty acid analog 11-((5-[dimethylaminoaphthalene-1-sulfonyl)amino)undecanoic acid (DAUDA), and by competition oleic acid. RT-PCR amplification of the hp-far-1 gene indicated that the gene is transcribed in all parasitic stages of the organism's life cycle. The presence of a secreted FAR ...
Molecular and Biochemical Parasitology
Ancylostoma secreted protein 2: cloning and characterization of a second member of a family of nematode secreted proteins from Ancylostoma caninum1999 •
International Journal for Parasitology
Molecular cloning and DNA binding characterization of DAF-16 orthologs from Ancylostoma hookworms2009 •
Parasites & Vectors
Interaction of hookworm 14-3-3 with the forkhead transcription factor DAF-16 requires intact Akt phosphorylation sites2009 •
The Biochemical journal
Diversity in the structures and ligand binding sites of nematode fatty acid and retinol binding proteins revealed by Na-FAR-1 from Necator americanus2015 •
Fatty acid and retinol binding proteins (FARs) comprise a family of unusual α-helix rich lipid binding proteins found exclusively in nematodes. They are secreted into host tissues by parasites of plants, animals and humans. The structure of a FAR protein from the free-living nematode Caenorhabditis elegans is available, but this protein (Ce-FAR-7) is from a subfamily of FARs that does not appear to be important at the host-parasite interface. We have therefore examined Na-FAR-1 from the blood-feeding intestinal parasite of humans, Necator americanus . The three dimensional structure of Na-FAR-1 in its ligand-free and ligand-bound forms, determined by nuclear magnetic resonance spectroscopy (NMR) and X-ray crystallography, respectively, reveals an a-helical fold similar to Ce-FAR-7, but Na-FAR-1 possesses a larger and more complex internal ligand binding cavity and an additional C-terminal a-helix. Titration of apo -Na-FAR-1 with oleic acid, analysed by NMR chemical shift perturbatio...
Journal of Biological Chemistry
Fatty Acid and Retinoid-binding Proteins Have Distinct Binding Pockets for the Two Types of Cargo2009 •
2015 •
BackgroundHookworms infect nearly 700 million people, causing anemia and developmental stunting in heavy infections. Little is known about the genomic structure or gene regulation in hookworms, although recent publication of draft genome assemblies has allowed the first investigations of these topics to be undertaken. The transcription factor DAF-16 is a mediator of multiple developmental pathways in the free living nematode Caenorhabditis elegans, and is involved in the recovery from the developmentally arrested L3 in hookworms. Identification of downstream targets of DAF-16 will provide a better understanding of the molecular mechanism of hookworm infection.MethodsGenomic Fragment 2.23 containing a DAF-16 binding element (DBE) was used to identify overlapping complementary expressed sequence tags (ESTs). These sequences were used to search a draft assembly of the Ancylostoma caninum genome, and identified two neighboring genes, snr-3 and lpp-1, in a tail-to-tail orientation. Expre...
2004 •
Ag-NPA-1 (AgFABP), a 15 kDa lipid binding protein (LBP) from Ascaridia galli, is a member of the nematode polyprotein allergen/antigen (NPA) family. Spectroscopic analysis shows that Ag-NPA-1 is a highly ordered, α-helical protein and that ligand binding slightly increases the ordered secondary structure content. The conserved, single Trp residue (Trp17) and three Tyr residues determine the fluorescence properties of Ag-NPA-1. Analysis of the efficiency of the energy transfer between these chromophores shows a high degree of Tyr-Trp dipole-dipole coupling. Binding of fatty acids and retinol was accompanied by enhancement of the Trp emission, which allowed calculation of the affinity constants of the binary complexes. The distance between the single Trp of Ag-NPA-1 and the fluorescent fatty acid analogue 11-[(5-dimethylaminonaphthalene-1- sulfonyl)amino]undecanoic acid (DAUDA) from the protein binding site is 1.41 nm as estimated by fluorescence resonance energy transfer. A chemical modification of the Cys residues of Ag-NPA-1 (Cys66 and Cys122) with the thiol reactive probes 5-({[(2-iodoacetyl)amino]ethyl}amino) naphthalene-1-sulfonic acid (IAEDANS) and N,N′-dimethyl-N-(iodoacetyl)-N′-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine (IANBD), followed by MALDI-TOF analysis showed that only Cys66 was labeled. The observed similar affinities for fatty acids of the modified and native Ag-NPA-1 suggest that Cys66 is not a part of the protein binding pocket but is located close to it. Ag-NPA-1 is one of the most abundant proteins in A. galli and it is distributed extracellularly mainly as shown by immunohistology and immunogold electron microscopy. This suggests that Ag-NPA-1 plays an important role in the transport of fatty acids and retinoids.
Σεραφείμ Μάξιμος: Αναμνήσεις
Σεραφείμ Μάξιμος: ένας διεθνικός επαναστάτης2021 •
Religion in Communist Lands
The Orthodox Church and the state in the Ethiopian revolution, 1974–841986 •
RSA Conference, Chicago. The Bellini of Renaissance Venice II, ed. by D. Maze
“Pater, patronus, benefactor et constructor”. Agostino Barbarigo and Giovanni Bellini in Murano2024 •
Lo specchio di Eva. Per una riflessione sulla violenza di genere.
LA VIOLENZA SESSUALE. DA REATO CONTRO LA MORALITÀ E IL BUON COSTUME A DELITTO CONTRO LA PERSONA. CONSIDERAZIONI GIUSFILOSOFICHE2024 •
2020 •
Journal of the Economic and Social History of the Orient |
Female Labor, Merchant Capital, and Resilient Manufacturing: Rethinking Ottoman Armenian Communities through Labor and Business2018 •
Clinical journal of surgery
Lumbar Hernia through an Iliac Crest Bone Graft Defect2018 •
2011 •
The Journal of Neuroscience
Mimicking Age-Associated Gadd45γ Dysregulation Results in Memory Impairments in Young Adult Mice2019 •
Revista de Historiografía
Instrumentos de difusión científica de la arqueología alicantina entre finales del siglo XIX y principios del XX (1886-1910): Las revistas El Archivo, El Ibero y Museo-Exposición2024 •
Applied and Environmental Microbiology
Importance of laccase in vegetative growth of pleurotus Florida1997 •
Revista de Saúde Pública
Influência das avós na prática do aleitamento materno2005 •
Journal of management info
The effect of perceived supervisory support on organizational commitment through employee engagement in banks in Sindh Pakistan2024 •
Applied Microbiology and Biotechnology
Elicitors’ influenced differential ginsenoside production and exudation into medium with concurrent Rg3/Rh2 panaxadiol induction in Panax quinquefolius cell suspensions2016 •
European Biophysics Journal
Colored noise in the fluctuations of an extended DNA molecule detected by optical trapping2011 •