polish journal of food and nutrition sciences
Pol. J. Food Nutr. Sci.
2008, Vol. 58, No. 1, pp. 113-117
www.pan.olsztyn.pl/journal/
e-mail: joan@pan.olsztyn.pl
POSSIBILITY OF PARTIAL REPLACEMENT OF FAT BY INULINE IN COOKIES IN ORDER TO
DECREASE THEIR CALORIC VALUE
Anna Żbikowska1, Jarosława Rutkowska2
1
Faculty of Food Technology, 2Faculty of Human Nutrition and Consumer Sciences, Warsaw University of Life Sciences
Key words: inuline, cookies, sensory properties, texture, trans fatty acids
Effects of inuline used as partial fat replacement in cookies containing three different fats were studied. During experiment sensory and instrumental attributes of reference cookies and low fat formulation in which 50% of fat was replaced with inuline were compared. Shortenings used for
preparation of cookies differed greatly in saturated fatty acids (SFA) (29.9–57.5%), trans fatty acids (TFA) (0.9–23.1%) and solid fat content (SFC)
at 25°C (13.6–31.6%). Replacement of 50% fat with inuline in the formulations enabled obtaining samples with higher instrumental values of texture
and resulted in cookies which were harder and less crispy. Also the intensity of sweet taste was sharply reduced by decreasing the fat content of cookie
recipes. On the other hand, replacement of 50% of fat with inuline had a little impact on the intensity of buttery taste of the cookies. The lowest intensity
of buttery taste was scored in cookies containing shortening with the lowest content of TFA. Concerning the overall sensory quality, the results showed
that the replacement of 50% of fat with inuline resulted only in a moderate decrease in the acceptability of the overall sensory quality. The averaged
scores of overall sensory quality were generally high and ranged from 7.1 to 9.1. In conclusion, it has been demonstrated that inuline was suitable for
inclusion in cookies, as a fat replacer and as a functional ingredient.
INTRODUCTION
Many cookies and other sweet baked goods contain high
amounts of sugar and fat. From the point of view of nutritive
properties, excessive consumption of bakery products is not
recommended for it is linked with the intake of SFA and TFA
[Balas, 2001; Erp-Baart et al., 1998; Daniewski, 1998] which
are known to provoke an increase of LDL/HDL-cholesterol
ratio, with a consequent increase in the risk of cardiovascular
diseases [Mensik et al., 2003; Juttelstad, 2004].
The consumption of sweet bakery products in Poland
amounted nearly 8 kg/person/year (2002-2006 years) and
evidence suggests that this will remain stable, because these
products are important dietary components of mid-morning
and afternoon snacks and also their sweetness and marked
palatability are elements which promote their consumption
[Gouveia et al.2007].
Inuline is a storage polymer composed of β-2,1-linked
fructosyl moieties mostly with a terminal glucosyl residue.
It occurs in many plants such as in banana, wheat, onions
and chicory [Van Loo et al., 1995]. Inuline content ranges
from less than 1% in banana to more than 15% in chicory roots with the number of fructosyl residues (degree of
polymerisation, DP) extending from 3 to 250, depending
on the plant species and the plants’ life cycle [Kip et al.,
2005].
For about 20 years, inuline has also been available as
an ingredient for application in the food industry, where it
is used in a large variety of food products, both for their
technological and nutritional benefits. Its nutritional benefits
arise from the fact that inuline is a dietary fibre [Flamm at
al., 2001] and consequently has a low caloric value and that it
resists gastric acid and digestion in the human small intestine
[Roberfroid, 1999]. Consumption of inulins also leads to an
increase in the number of Bifidobacterium and Lactobacillus
species in the human fecal colon microbiota [Gibson et al.,
1995]. Based on a combinations of these properties inuline
finds applications in, for instance, dairy products, beverages,
spreads, ice cream, confectionery, bread and other bakery
products.
The aim of this study was to examine the effect of partial
replacement of some fats with inuline in cookies on their sensory and instrumental quality indices.
MATERIALS AND METHODS
MATERIALS
Experimental cookies were prepared using a recipe containing high amount of fat (55% with respect to flour). It
is a typical formula of short-dough biscuits mostly used in
manufacturing cookies in Poland. Three different shortenings
were used for the preparation of reference cookies: Akobake
K, Akobake P (Karshamns, Sveden) and Cargill shortening
(Cargill, the Netherlands). All are based on fractions of palm
oil. In low fat formulation 50% of fat was replaced with inuline.
Author’s address for correspondence: Jarosława Rutkowska. Department of Food Analysis, Quality Assessment, Warsaw University of Life Sciences,
ul. Nowoursynowska 159 C, 02-776 Warszawa, Poland; tel./fax: (48 22) 59 37 071; e-mail: jaroslawa_rutkowska@sggw.pl
© Copyright by Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
114
Ingredients for preparing cookies
The reference cookie formulation contained the following
ingredients: 240 g of wheat flour, 133 g of fat, 90 g of powdered sugar, 76 g of egg yolks, 10 g of dried milk. In low fat
formulation 50% fat was replaced with 17 g inuline (Hortinex). This corresponds to Hortinex recommendations according to which 1 g of inuline replaces 4 g of fat.
Preparation of cookies
All ingredients were blended in a Philips mixer for 6 min.
Inuline was added directly to the blended fat and mixed with
other ingredients. Thereafter, the mixed dough was rolled out
and cut in square pieces 55 mm in length and 4 mm in thickness. The cookies were placed into baking cups and baked
at 190°C for 12 min in a Sveba Dahlin Fristad Sveden oven.
The prepared cookies were subjected to instrumental and sensory analysis 24 h after baking.
METHODS
Analysis of fats
Gas chromatography (GC) was used to determine FA
composition of the investigated fats according to the Polish
Standard [PN-EN ISO 5508:2000]. Methyl esters were prepared according the Polish Standard [PN-ISO 5509:2000].
The composition of FA was expressed as the peak area
percentage of total FA. Instrument: HP 6890 GC System
with autosampler; SGE Capillary BPX 70, column: 60 m x
0.25 mm ID; oven: temperature program from 160 to 210°C,
rate: 2.5°C/min; carrier gas: helium, air: 300 mL/min; injector: Split-Splitless 240°C; detector: FID 250°C; software: HP
Chemstation v. 3.11. Method validation was carried out with
a reference sample CRM-163 EEC-EAEC (Brussels, 1993).
SFC of fats was determined by means of pulsed nuclear magnetic resonance spectroscopy using NMS 120 Minispec NMR
Analyzer Bruker instrument at 25°C. Melting point (MP) was
estimated with the capillary method following the Polish Standard [PN-EN ISO 6321:2004]. According to the standards,
analyses of fats were conducted in two replications.
Sensory evaluation of cookies
Sensory analysis of cookies was carried out using the scaling method by a sensory panel according to Polish Standard
[PN ISO 4121:1998]. The sensory panel of ten members,
habitual consumers of bakery products (students and faculty
staff members: 24–46 years of age), made each evaluation in
duplicate. They scored all sensory attributes on a 100 mm
unstructured scale and converted the scores after collection
into 0-10 conventional units scale. They were asked to taste
each cookie and rated intensity of buttery aroma, sweet taste
and buttery taste of each sample. The scales were anchored
at each extreme with “not at all ….” and “extremely ….”. The
subjects rated acceptability of appearance and attributes of
cookies texture: hardness, crispness and greasiness and overall quality. The scales were anchored at extreme “low…” and
“high…”. Only with respect to the greasiness the scales were
anchored at extreme “not at all …” and “extremely …”. measured using a linear scale. Samples of cookies were coded and
given to each assessor in individual random order.
A. Żbikowska & J. Rutkowska
Instrumental analysis of cookies
Texture measurements were carried out using Texturometr
Model TA-XT2 (Stable Micro Systems, UK) by a bending-snapping test according to Baltsavias et al. [1999]. Procedure: broken shear force of each cake was determined as an
indication of hardness and crispness. A 10 kg load cell was
used and crosshead speed set at 1 mm/min, temperature 23°C.
Volume of cookies (in cm3) was determined using Jakubczyk
& Haber method [1983].
Data were analysed with the use of Statgraphics plus 4.1.
Duncan’ s test was used to assess the differences between
means at p<0.05.
RESULTS AND DISCUSSION
Fats
The results of GC analysis of fats are presented in Table 1.
Shortenings differed in the content of SFA which ranged from
29.9 to 57.5%. Generally, the levels of SFA in all used fats were
high, which is typical of bakery fats because SFA are resistant
to oxidation and on the whole have excellent technological
properties [Krygier & Żbikowska, 2002]. Shortenings differed greatly in TFA content which are regarded as especially
undesirable in the diet [Pedresen, 2001]. However, TFA have
an excellent effect on the structure of bakery products. Many
studies revealed that bakery and confectionery fats are the
crucial sources of TFA [Balas, 2001; Daniewski et al., 1998;
Erp-Baart et al., 1998; Parcerisa et al., 1999].
From the three shortenings studied Cargill fat was characterised by both the lowest content of TFA (0.9%) and the
highest content of SFA (57.5%). As expected, the studied fats
contained low amount of polyunsaturated FA (PUFA) (5.9 to
0.5%) (Table 1). Unsaturated FA are undesirable in bakery fats
because they are not resistant to environmental stress during
the baking process (high temperature). At high temperature
unsaturated FA, especially α-linolenic acid (C 18:3) are the
main source of undesirable oxidation products.
There were no significant differences in MP between shortenings. In contrast, fats differed in SFC content (13.6-31.6%),
(Table 1).
Sensory and instrumental evaluation of cookies
Flavour, texture and appearance are the main quality attributes of cookies. Fat is a very important ingredient of cookies
because it contributes to the texture and pleasing mouthfeel
and additionally positively impacts flavour intensity and perception [Zoulias et al., 2002]. Sensory intensity of aroma,
TABLE 1. Characteristics of the examined fats.
Parameter
Brand of fat
Akobake K
Akobake P
Cargill
SFA (%)
29.9±0.89
49.1±4.23
57.5±4.10
TFA (%)
23.1±0.90
3.2±0.10
0.9±0.21
2.3±0.30
5.9±0.71
0.5±0.20
PUFA (%)
Sum of cis isomers (%)
53.0±3.89
52.3±3.85
56.4±4.12
SFC at 25°C (%)
13.6±0.85
18.2±0.92
31.6±0.91
SMP (°C)
34.6±0.92
35.8±0.98
35.5±0.93
115
Partial replacement of fat by inuline in cookies
TABLE 2. Results of sensory evaluation of cookies (scale 0-10).
Intensity
Sample
Buttery Sweet Buttery
aroma taste
taste
TABLE 3. Results of sensory assessment of texture attributes of cookies
(scale 0-10).
Appearance
Overall
acceptquality
ability
Attributes of texture
Samples
Hardness
Crispness
Greasiness
b
3.4a
d
Akobake K
2.7b
5.1b
4.1d
6.6bc
9.1c
Akobake K
5.3
5.7
Akobake K + inuline
b
2.1
3.6
cd
c
b
Akobake P
b
2.5
5.7
bcd
b
3.6
6.8
7.8
Akobake K + inuline
8.3
3.1
5.6b
c
3.5
5.1
a
b
8.1
Akobake P
3.9a
6.8c
3.9a
b
b
b
a
a
Akobake P + inuline
2.0
4.6
3.4
6.2
8.1
Akobake P + inuline
8.2cd
2.9a
5.5b
Cargill
1.8a
5.3bc
2.8ab
7.3d
8.9c
Cargill
4.0
c
7.5
5.5b
Cargill + inuline
1.2
3.7
2.5
6.7
7.1
Cargill + inuline
7.6c
3.3a
6.1b
NIR
0.81
0.79
0.69
0.50
NIR
0.78
0.83
0.85
a
a
a
bc
a
c
0.74
a
a
The same letters denote non-significant differences (p<0.05) between
mean values in the same column.
The same letters denote non-significant differences (p<0.05) between
mean values in the same column.
intensity of taste, acceptability of appearance and overall
ratings averaged over the six types of cookies are presented
in Table 2. The statistical comparison was made between the
control cookies containing three different fats and the three
with the lower fat content containing inuline.
The lowest intensity of buttery aroma was rated in cookies
containing shortening with 57.5% SFA and 0.9% TFA (Cargill)
for both inuline-containing and control recipes. Replacement
50% of fat with inuline had significant (p<0.05) impact on the
intensity of buttery aroma of cookies containing shortening
with 49% SFA and 3.2% TFA (Akobake P) (from 2.5 to 2.0).
In the case of Akobake K or Cargill shortenings, the effect of
fat replacement with inuline was not statistically significant
(p<0.05). Generally, the sensory panel found low intensity of
buttery aroma in all samples.
Appearance and taste are highly relevant to the consumer
preference of foods and are particularly important, in the case
of health-promoting foods, in those groups most inclined to
unhealthy diets [Neumark-Sztainer et al., 1999]. This conclusion may be extrapolated to functional foods and therefore,
in our study, an assessment has been made of sensory intensity in sweet and buttery taste in control cookies and cookies with 50% of fat replaced with inuline. The intensity of
sweet taste was sharply reduced by reducing the fat content
of cookie recipes (Table 2). It was particularly evident when
shortening with the lowest TFA content (0.9%) was utilized
(decreasing intensity of sweetness of cookies from 5.3 to 3.7
was attained). Similar results were presented by Drewnowski
et al. [1998] in cookies with 25% reduced sugar content and
confirmed that the key sensory attribute for this class of food
products is sweetness and that consumers are very sensitive to
any variations in sugar content.
Replacement of 50% of fat with inuline had a little impact on the decreasing intensity of buttery taste of cookies.
The lowest intensity of buttery taste was scored in cookies
containing shortening Cargill (with the lowest content TFA).
With respect to using shortening Akobake K, the sensory
panel did not find differences (p<0.05) in the appearance between control cookies and those containing inuline. Replacement of 50% of Cargill with inuline significantly (p<0.05)
decreased the scoring of appearance, whereas the cookies
containing inuline with shortening Akobake P attained higher
scores with respect to acceptability of appearance than the
control samples (5.1 and 6.2, respectively).
With respect to cookies containing Akobake K and Cargill,
the estimation of overall sensory quality (Table 2) confirmed
lower acceptability of cookies in the case when 50% of fat
was replaced with inuline. With respect to Akobake P no significant differences were found between control cookies and
those containing inuline. Generally, the averaged scores were
high and ranged from 7.1 to 9.1 (on the scale 0-10 cm).
Texture of cookies
The importance of cookies texture in consumer acceptance is increasingly recognized. Fat is one of the principal
ingredients that affect cookies texture. Substitution of fat with
other ingredients had a greater impact on textural attributes
of cookies than the replacement of sugar or flour [Campbell
et al., 1994]. It can be seen (Table 3) that reducing the fat content by 50% and replacing it with inuline in cookie recipes led
to higher ratings of hardness. The same results were obtained
during instrumental measurement of texture (Table 4).
In all samples replacement of fat with inuline resulted in
2-fold increase of instrumental values of texture. For example,
TABLE 4. Results of instrumental measurements of physical properties of cookies.
Physical
property
Volume (cm3)
NIR = 1.05
Texture (N)
NIR = 3.18
Sample
Akobake K
Akobake K + inuline
Akobake P
Akobake P + inuline
Cargill
Cargill + inuline
30.1
28.9
33.3
31.6
e
35.5
30.4b
15.6a
37.4d
14.0a
39.9d
12.6a
29.8c
b
a
d
c
The same letters denote non-significant differences (p<0.05) between mean values in the same line.
116
instrumental texture of cookies containing shortening Akobake K was 15.6 N and after replacement of 50% of fat with
inuline it increased to 37.4 N. In several studies it is pointed
that high values of shear force (which indicated hardness) is
an unpleasant attribute for such type of product [Zoulias et
al., 2002; Rutkowska, 2003].
Crispness can be considered as a pleasant sensory property of cookies as far as it does not become extremely great
[Zoulias et al., 2002]. Sensory panel found reference cookies
crispy (5.7–7.5 points), which was strongly reduced after replacing 50% fat with inuline (2.9–3.3 points).
Decrease in crispness was associated with higher hardness. The above results obtained are similar to those described in the literature. For example, Maache-Rezzoug et al.
[1998] investigated the effect of fat content on biscuit texture
and found that an increase in fat content in biscuits resulted
in more friable and crispy products. Reduction of fat in cookies resulted in a chewy texture and decreasing moisture content [Sanchez et al., 1995]. Greasiness is a texture attribute of
cookies perceived during mastication. For cookies containing
shortenings Akobake K or Akobake P replacement of 50% of
fat with inuline significantly increased the scoring of greasiness. In the studies on the texture of crackers Martinez et al.
[2002] found that high intensity of greasiness was not acceptable by consumers.
Instrumental estimation of the volume of cookies revealed
decreasing values as a result of replacing 50% fat with inuline. The differences in volume between two types of cookies
studied did not exceed 15%. During evaluation of the acceptability of appearance the analytical panel highly scored the
lower volume of cookies containing inuline (from 6.2 to 6.7
scores).
CONCLUSIONS
Reduction of fat in the formulations led to samples with
higher instrumental values of texture, therefore resulted in
cookies which were estimated as more hard.
On the other hand, the replacement of 50% of fat with
inuline had various effects on the sensory properties of the
samples depending on the shortening used:
• Intensity of sweet taste was significantly reduced by decreasing the fat content of cookie recipes. It was particularly
evident when shortening with the lowest TFA content (0.9%
Cargill) was utilized.
• Replacement of 50% of fat with inuline had a significant
impact on intensity of buttery aroma of cookies containing
shortening with 49% SFA and 3.2% TFA (Akobake P). Generally, the sensory panel found low intensity of buttery aroma
of all samples.
• Replacement of 50% of fat with inuline had a little impact on the intensity of buttery taste of cookies. The lowest
intensity of buttery taste was scored in cookies containing
shortening with the lowest content of TFA.
• The replacement of 50% of fat with inuline resulted only
in a moderate decrease of the acceptability of overall sensory
quality.
These data have some implications for designing acceptable lower-energy cookies. Sweetness is a desirable sensory
A. Żbikowska & J. Rutkowska
attribute in this group of food products. As a result, any manipulations that reduce the sweetness level of cookies will
result in their diminished acceptance. On the other hand, reducing the fat content in cookies will decrease the total and fat
calories, and replacing part of fat with inuline will influence
the nutritional benefits.
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Received March 2007. Revision received September and accepted
October 2007.
MOŻLIWOŚCI ZASTĄPIENIA INULINĄ CZĘŚCI TŁUSZCZU W CIASTKACH W CELU OBNIŻENIA ICH
WARTOŚCI KALORYCZNEJ
Anna Żbikowska1, Jarosława Rutkowska2
1
Katedra Technologii Żywności, 2Zakład Analizy i Oceny Jakości Żywności, Szkoła Główna Gospodarstwa Wiejskiego w Warszawie
Celem pracy było określenie możliwości zastąpienia inuliną części tłuszczu w ciastkach kruchych z dodatkiem trzech różnych tłuszczów. Porównano sensoryczne i instrumentalne wyróżniki jakości ciastek, w których 50% tłuszczu zastąpiono inuliną z próbami kontrolnymi. Szorteningi
użyte do wypieku eksperymentalnych ciast różniły się zawartością SFA (29,9–57,5), TFA (0,9–23,1%) oraz SFC w 25°C (13,6–31,6%). Zastąpienie
w recepturze 50% tłuszczu inuliną powodowało wzrost wartości siły łamania ciastek mierzonej instrumentalnie co było postrzegane w ocenie
sensorycznej jako wzrost twardości i spadek kruchości produktów (tab. 3, 4). Podobnie obniżenie tłuszczu w recepturze wyraźnie spowodowało
spadek intensywności smaku słodkiego. Z drugiej strony, zastąpienie w ciastkach połowy tłuszczu inuliną w niewielkim stopniu wpływało na sensoryczną ocenę intensywności maślanego smaku (tab. 2). Najniższą intensywność smaku maślanego stwierdzono w wyrobach zawierających
tłuszcz z najmniejszą zawartością TFA (0,9%). Również sensoryczna jakość ogólna ciastek wykazała tylko umiarkowany spadek tego wyróżnika
gdy 50% tłuszczu zastąpiono dodatkiem inuliny. Średnie wartości ogólnego wyróżnika jakości sensorycznej były wysokie i wynosiły od 7,1 do 9,1
punktów (tab. 2). Uzyskane wyniki potwierdziły informacje z piśmiennictwa o tym, że inulina może być zastosowana w ciastkach zarówno jako
dodatek obniżający ich wartość energetyczną jak i dodatek funkcjonalny.