!
"
$
! #
$ !
"
! "
Faculty of Pharmacy, Medical University of Sofia, Dunav Str. 2, 1000 Sofia, Bulgaria
* Corresponding author. I. Ionkova E-mail address: ionkova@pharmfac.acad.bg
# $
$
+ - ./
)(
+'
!"#$
%
%
)
- ./
%
$*'
!&'(&&
%
'
'+'
)
%
$*', '
!&
)
)
%
'$
&
0 1
() $ '
%
- ./
)
$*', '+'
'
'
%
6:'24
; 1
<1'+
=
2
-
)
%
%
$)
;
'
!)
$*', '+ - ./
()
3
)
5
)
4 2
5
1
%
%
%
%
%
6 7
$*'
$*', '+ - ./
+ - ./
89
)
'+'
+'
>
$*', '+ - ./
)
)
#)
$*, '+ - ./
2
)
(
#)!
+)
?
)
$*', '+'
%
!
)
" #
$ %%%
=
102
%
!
D
)
(
)
)
>
)5
%
("
)
!&'(&&
* $
)
'( * $
+
6.9
)+$
(
H&)&!
'( ( )
I#)
'
<
>
9
4
;
: : &&&
:
8
%
>
(&&$)
$
5
6) 9
5
2
!
%
4
6
( J)
)
@) 1
2
)
+)
D
>
!"#$
)
)
!)
%
&)(
%
(
#&
)
) ,
+
;
!)&
?
'
()
β'
)
#! A
%
)
)
B "
;-1
E
C
;-1
) <
)
D
=
)5
(& .,5'!5
$)+
) )
F E
4
@
"
)&
1?
1?
<
& !&
$
&&
)
2
)
E7. '5<1
;
0&
$&G 4
$&
($
&)" 1?
@)&
"
($
)
+@G 4
2
&
()
7
;7
+ - ./
+ - ./ ! )
+ - ./
&) "&
+ - ./
?
&
' $*, '
&&
$*, '
2
)
!
) <
) )
E
@
%
!& (&&
(
#
2
2
(!&
$)+
$&
$&G 4)
&)"
'
&
@& A
2
(
" #
$ %%%
%
&) (!&
?
)
%
103
Table 1: Effects of Methyl jasmonate, on the production of
Lignans in L. tauricum-Hairy roots (ATCC 15835), clone
Lt-A12
4'-DMMJ concentration in
6-MPTOX
MPTOX
medium
(mg/g dry
(mg/g dry
(microM)
weight)
weight)
0
25.92±0.31
35.06±0.03
50
26.09±0.17
35.11±0.28
100
29.19±0.11
35.42±0.19
150
31.92±0.15
36.19±0.08
200
20.30±0.33
28.12±0.21
*Data was taken after 4 weeks of culture
$*', ' - ./
# )J(
?
,3 ) ;
)
!&
) 5
&)
=
$*, '+ - ./
+ - ./
)
'
' +)
$*', '+ - ./ $ )
1
)
D
)(
2
- ./
$*', '
'
# )J(
,3
(!)J(
'
) <
%
'
!"#$
,3
- ./ #+) J
)
!& M
,3
)
7
1'
(
(
'
%
("
)E
)
)
%
$*', '
+ - ./
%
D
!& (&&
)
' & !&
)&
)&K
&&
=
)
(
#
2
%' ,+ ( * '$ -
)
&)((
>
:
5
)
@
)
5
2
.
7
1.
)
2.
%
, '+ - ./
!&
)
$*'
)(
# )J(L&) !L&) !
?
)
+ - ./
'
#+) JL&)&"
!
)
" #
3.
4.
?
)
$ %%%
4
D
<) <
2
)
'
H. F. Stahelin and A. V. Wartburg, The chemical and
biological route from podophyllotoxin glucoside to etoposide,
CancerRes. 51 5-15, (1991).
I. Ionkova. Biotechnological Approaches for the Production of
lignans. Pharmacognosy Review. 1: 57-68, (2007).
B. Konuklugil, I. Ionkova, N. Vasilev, T. Schmidt, J.
Windhövec, E. Fuss, and A. Alfermann. Lignans from Linum
species of sections Sylinum and Linum. Nat. Prod. Research.
21: 1-6 (2007).
N. Vasilev, G. Momekov, M. Zaharieva, S. Konstantinov, P.
Bremner, M. Heinrich and I. Ionkova. Cytotoxic activity of a
podophyllotoxin-like lignan from Linum tauricum Willd.
Neoplasma. 52: 425-429 (2005).
104
5.
6.
7.
Ionkova I., E. Fuss, Influence of different strains of
Agrobacterium rhizogenes on induction of hairy roots and
lignan production in Linum tauricum ssp. tauricum,
Pharmacognosy Magazine, vol. 4, 17: 14-18 (2009).
Zhao J., Lawrence C. and Verportee, R., Elicitor signal
transduction leading to production of plant secondary
metabolites, Biotechnology Advances, vol. 23, 4: 283-333,
(2005).
Murashige T. and Scoog F. A revised medium for rapid growth
and bio assays with tobacco tissue cultures. Physiol. Plant. 15:
473-497 (1962).
8.
I. Ionkova, T. Kartnig and W.Alfermann. Cycloartane saponin
production in hairy root cultures of Astragalus mongholicus
Bge., Phytochemistry. 45: 1597-1600 (1997).
9. Verpoorte, R., van der Heijden, R., ten Hoopen, H.J.G.,
Memelink, J., Metabolic engineering of plant secondary
metabolite pathways for the production of fine chemicals.
Biotechnol. Lett. 21: 467–79, (1999).
10. I. Ionkova, B. Goermova, N. Vasilev, E. Fuss, Lignan
production in cell cultures of Linum tauricum ssp. tauricum,
Phytologia Balcanica, in press, (2009).
NNNNNNNNNNNNNNN
!
" #
$ %%%
105