JJBS
Volume 6, Number 3, September .2013
ISSN 1995-6673
Pages 205 – 210
Jordan Journal of Biological Sciences
Is Gaza Sandy Shoreline Region Contaminated with Human
Gastrointestinal Parasites?
Ahmed H. Hillesˡ , Adnan I. Al Hindi², * and Yousef A. Abu Safieh³
54T
P
P14T50F
P14T5
1
P
Institute of Water and Environment, Al Azhar University ,²Department of Biology, Faculty of Science, The Islamic University of Gaza,
P.O.Box 108, Gaza,³Ministry of Environment Affairs, Palestinian National Authority,Palestine
P
47T
47T
Received: January 30, 2013; accepted: March 20, 2013
Abstract
The study was implemented to test if the sandy shoreline of Gaza city is contaminated with human gastrointestinal
parasites or not and to determine the types of intestinal parasites and the extent of contamination. A total of 104 sand
samples (52 dry sand and 52 wet sand) were analyzed during the summer season period. Samples were collected from the
study area of about 12km along the seashore region of Gaza City. Dry samples and wet sand samples were analyzed using
water-sedimentation technique and a light microscope. The results showed that the percentage of the parasitic
contamination was 40.4% of the wet sand samples and 34.6% of the dry sand samples along the shoreline region of the
Gaza City. The human gastrointestinal parasites detected were the following: Ascaris lumbricoides, S. stercoralis, E.
vermicularis, E. histolytica/dispar, G. lamblia, E. coli and Taenia. spp. The findings showed that there was no statistically
significant difference in the concentration of parasitic contamination between the dry and wet sand samples at confidence
level of p-value < 0.05. It is recommended to conduct a periodical routine sampling of sand at the swash zone because the
results of this study showed that the wet and the dry sand may pose a high level of health risk. Residents should be
informed clearly by posting signs indicating polluted areas to keep them safe.
Keywords: Sandy shoreline, Intestinal parasites, Contamination, Wastewater, Gaza city
1. Introduction
The population of Gaza City is about 552,000 (PCBS,
2011), and receives a water supply through the Coastal
Municipalities Water Utility (CMWU) water supply
system. High percentage of the wastewater that is
generated in Gaza City is currently discharged without
sufficient treatment into the sea in addition to the Wadi
Gaza’s wetland effluent channel for the raw sewage from
refugee camps adjacent to the watercourse, estimated of
about 6-8MLD (Hilles and Abu Amr, 2010).
The population of Gaza Strip continues to grow
rapidly, which increase the amounts of poorly treated or
untreated sewage being discharged into the coastal water.
With the Palestinian population growth rate of around
4.8% per annum, which would result in a doubling of the
population in 15 years, effective management and
sustainable development of Gaza resources will be a huge
challenge for the Palestinian Authority (UNEP, 2003).
In Gaza City, there is only one insufficient and
inefficient wastewater treatment plant (GWWTP) which
is considered to be the largest in Gaza Strip. Insufficient
means that the quantity of the wastewater discharged from
*
Corresponding author. e-mail: ahindi@iugaza.edu.ps.
the city and arrived to the plant exceeds its capacity,
while, inefficient means that the plant suffers from lack of
maintenance and operational problems. GWWTP
discharges about 50MLD of partially treated wastewater
directly into the sea along with 10MLD of untreated
wastewater (raw sewage) is currently discharged directly
into the sea of Gaza City (EWASH, 2009).
The only study conducted in Gaza was the assessment
of total coliform and faecal coliform in Gaza sea shore
(Elmanama, 2004). The present study is the first one to
assess the parasitic contamination of Gaza sea shore.
The lack of sufficient wastewater treatment facilities
makes wastewater which discharges into the sea the main
source of pollution of the coastal zone of Gaza Strip.
There are more than 20 individual sewage drains, ending
either on the beach or a short distance away in the surf
zone. Insufficient number of sewage treatment plants in
operation, combined with poor operating conditions of
available treatment plants, and the present disposal
practices are likely to have an adverse effect on the
quality of seawater (EQA and UNEP, 2005).
The main aim of the current study is to examine if
Gaza shoreline region is contaminated with human
gastrointestinal parasites (identify them up to the species
206
© 2013Jordan Journal of Biological Sciences. All rights reserved - Volume 6, Number 3
level ) and to determine the extent of parasitic pollution
in the seashore sand..
2. Materials and Methods
2.1. The study area:
About 12km of Gaza City shoreline was divided into
six sampling zones in order to facilitate the sampling
process as it is shown in table 1 and Fig 1.
2.2. Sampling processes
Sampling were conducted throughout the summer
season months (from June to October, 2011).During the
study 500gm of sand were collected from every sample of
the previously mentioned areas (Table 1 and Figure 1) of
2m², which were divided into five cores of 100gm taken
from each corner of the sampling site and another one
from the central part of the area within the superficial
layer of the ground at an approximate depth of 5-10cm
(Colli et al., 2010). The samples were stored in suitable
plastic bags, and directly labeled and signed with special
water-resistant pen. Dry sand samples were collected
from non flooded areas (above high tide line) in the areas
out of reach of seawater, and wet sand sample was taken
from an intermediate area between the dry sand area and
the seawater (Swash zone or Intermediate zone) where it
was very close to the seashore and the seawater usually
reach and moisten the sand in these sites, and where
bathers could be found most of the time.
Table 1. Zones of Sampling and Related Information
Zone
Symbol
Zone
Boundaries
Zone
length
A
From Wadi Gaza to Al-Zahra
City
1800m
B
C
D
Al-Zahra City to Al-Baydar
resturant
From Al-Byder restaurant to
Khalel Alwazer mosque
From Khalel Alwazer mosque to
the southern part of the Gaza
marina
2000m
1800m
Total number of samples
(24 Samples)
-First 5 samples every50m.
-Second 5 samples every 250m.
(10 samples)
-Every 500m
(12 samples)
- Sample every 300m.
Number of samples
Wet
Dry
Sand
Sand
12
12
5
5
6
6
2500m
(24 samples)
- Sample every 200m.
12
12
E
The basin of the Gaza marina
450m
(8 samples)
-Every 100m.
4
4
F
From the northern part of the
Gaza marina to the Intelligence
Building
2800m
(26 samples)
- Sample every 200
13
13
Total
Study area
12km
104 samples
52
52
Figure 1. Distribution of the detected parasites in the dry sand within the six zones
207
© 2013Jordan Journal of Biological Sciences. All rights reserved - Volume 6, Number 3
2.3. Using water-sedimentation technique,
35gm of sand was diluted (washing gently for 15 sec)
in 150 ml of distilled water, filtered through a sieve
meshes (75μm) and allowed to settle for between six and
eight hours in a suitable 250 ml measuring cylinder. Two
ml of the surface of the cylinder (floated parasites in the
upper part of the aqueous solution) were taken and
discarding the rest of the supernatant, about eight ml of
the stagnant sediment were collected, and the two
amounts were centrifuged together to concentrate the
sample by 1500 rpm for about 10 min and a concentrated
sediment was collected (Colli et al., 2010).
The sand analysis process was completed by applying
the following steps: One drop of the sediment was placed
in the center of the slide. The drop was covered with a
cover slip by holding the coverslip at an angle, touching
the edge of the drop, and gently lowering the coverslip on
top of the slide so that air bubbles are not produced. The
slide was examined with 10X objective or, when needed
for more identification, higher power objectives of the
microscope have been applied in a systematic manner
(either up and down or laterally) so that the entire
coverslip area was surveyed. When organisms or
suspicious objects were seen, switching to higher
magnification was necessary to see more detailed
morphology of the object in question. The sediment were
stored in a labeled suitable tube which is known as
opened rove (with a sharp bottom and snap cap) for
further analyses and inspection.
3. Results
The present study showed a diversity of human
gastrointestinal parasites in both dry and wet sand in Gaza
sandy shoreline region.
The results of the dry and wet sand analysis for the
entire study area (six zones = 52 samples) are shown in
Table 2. Eighteen (34.6%) were found to be contaminated
with human gastrointestinal parasites. It was found that 14
samples were contaminated by one type of parasites
(single) and 4 samples were contaminated by several
types of parasites (mixed).
For wet samples, 21 samples (40.4%) were
contaminated by human gastrointestinal parasites: 18
samples were contaminated by one type of parasites
(single) and 3 samples were contaminated by more than
one species (mixed) of human gastrointestinal parasites as
shown in Table 2.
Figure 1 shows that the dry sand samples were
contaminated
with
seven
species
of human
gastrointestinal parasites, distributed in order as follows:
39.1% Ascaris lumbricoides, 21.7% S. stercoralis, 13.1%
E. vermicularis, 8.7% E. histolytica/dispar, 8.7 % G.
lamblia, 4.3 % E. coli and 4.3 % Taenia. spp.
According to Figure 2, the results of contamination
with parasites in dry sand according to the different six
zones shows that zone (A) has the highest level of
contamination with 44.4% of polluted samples, followed
by 22.2% in zone (D), 22.2% in zone (B), 5.6% in zone
(C), and finally 5.6% in zone (E). No parasitic pollution
was detected in zone (F).
Figure 3 shows that wet sand samples were
contaminated with six species of human gastrointestinal
parasites and distributed as follows: 58.2% S. stercoralis,
25.0% A. lumbricoides and 4.2% for each of E.
histolytica/dispar, G. lamblia, E. coli and Taenia. spp.
As illustrated in
Figure 4, contamination with
parasites in wet sand depending on the different six zones
shows that zones (A) and (D) have the highest level of
contamination with 28.6% for each zone, followed by
19.0% in zone (C), 14.3% in zone (B) and finally by 9.5%
in zone (E). No parasitic pollution was found in zone (F).
The images of the human gastrointestinal parasites
which have been recorded and identified in the samples
from the six zones and all sampling sites are presented in
Figure 5.
No statistical significant difference in the
concentration of parasitic contamination between the dry
and wet samples at a confidence level of a p-value < 0.05
was found (Table 3.).
Table 3 illustrates the results of a single factor one
way-ANOVA test for the spatial variation in the parasitic
contamination within the six different zones along the
entire study area to examine whether there is a significant
statistical difference in the contamination level through
those zones and the level of significance. The results
indicate that there is a significant variation among the
zones within the confidence level of a p-value of < 0.05).
Table 3 shows a significant mean difference between
the higher polluted zone (A) and the other zones. Also,
there was a significant mean difference between
uncontaminated zone (F) compared to the other zones
using multiple comparisons (LSD) as a statistical analysis
method.
Table 2. Percentage of Contaminated Dry and Wet Sand Samples
Dry Sand
No.
Wet Sand
(%)
No.
(%)
Single
14
18
Mixed
4
3
Total contaminated samples
18
34.6
21
40.4
Uncontaminated
34
65.4
31
59.6
Total samples
52
100.0
52
100.0
Contaminated
samples
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© 2013Jordan Journal of Biological Sciences. All rights reserved - Volume 6, Number 3
Table 3. Statistical analysis done during the study
t-Test for the Means of the Dry and Wet Sand
Independent Samples Test
Levene's Test for Equality of Variances t-test for Equality of Means
Equal variances assumed
F
Sig.
t
df
Sig. (2-tailed)
1.399
0.240
0.603
102
0.548
0.603
101.903 0.548
Equal variances not assumed
2. One way –ANOVA Test for the Parasitic Pollution within the Six Zones (A, B, C, D, E, F)
Sum of Squares
df
Mean Square
Between Groups
11.133
5
2.227
F
p-value
Within Groups
43.467
254
13.011
.001
Total
54.600
259
.171
3. Multiple Comparisons (LSD) for the Parasitic Pollution Within the Six Zones
(I) ZONE
(J) ZONE
Mean Difference (IJ)
Std. Error
p-value
A
B
.2300(*)
.09848
0.020
C
-.1397
.09356
0.137
D
-.1057
.07522
0.161
E
-.2500(*)
.10681
0.020
F
-.4500(*)
.07406
0.001
A
.4500(*)
.07406
0.001
B
.6800(*)
.09736
0.001
C
.3103(*)
.09238
0.001
D
.3443(*)
.07374
0.001
E
.2000
.10578
0.060
F
28.6%
28.6%
19.0%
14.3%
9.5%
30%
20%
0.0%
10%
0%
A
Figure 2. Percentages of contamination in dry sand according to
the six zones
Figure 3. Distribution of the detected parasites in the wet sand
within the six zones
B
C
Zones
D E
F
Figure 4. Percentages of contamination in the wet sand
according to the six zones
© 2013Jordan Journal of Biological Sciences. All rights reserved - Volume 6, Number 3
Figure 5. Images of Human Gastrointestinal Parasites
4. Discussion
The present study focused on the contamination of
sandy shore of Gaza City, the detected human
gastrointestinal parasites in the sandy shore considered as
evidence of contamination. It is clear that the observed
discharge points reach the sandy beach are the main
source for the existence of the detected intestinal parasites
where moisture and suitable temperature are available.
Sandy soils represents an important source of human
infection by parasites, due to their geological
characteristics, being formed by sand particles with
diameters ranging from 0.02 to 2 mm, and with the ability
to retain water between the spaces of soil particles (Rocha
et al., 2011).
In the same regard it is very important to mention that
since wastewater treatment plants in Gaza strip are
partially active, so untreated sewage is discharged to the
Mediterranean Sea directly. Fathers reach sandy beaches
with their children where they are digging in the sand, not
knowing they will be exposed to contaminate their hands
with parasites, according to the OPAS 2002 report, it is
estimated that two billion people in the world are infected
by some form of parasites acquired through the contact
209
with soil, 800 million of the infected are children (40%)
(Da Silva et al., 2012).
Very little information exists concerning the presence
of viruses and parasites in the beach sand. In a three-year
study in Romania by Nestor et al. (1984), the incidence of
parasites was found to depend on season, during nonvacation seasons no parasites being present in seawater
and beach sand. In a study of two sand beaches in
Marseilles, France, Toxocara canis was found to be the
most common parasite, being present on average in 150 g
of sand (Signorile et al., 1992). However, in a study
carried out on “dog beaches” in Perth, Australia, a total of
266 samples showed no traces of Toxocara canis eggs or
other eggs/larvae of parasitic nematodes (Dunsmore et al.,
1984). It was emphasized in that study that the major risk
to humans was from an environment in which puppies,
not older dogs, were found. The presence of other
parasites transmitted by water (Marshall et al., 1997) that
have not been investigated in recreational sand areas may
be potentially significant.
The evidence of contaminated Gaza sandy shoreline
region has been supported by many studies. Beaches
represent the unconsolidated sediment that lies at the
junction between water (oceans, lakes and rivers) and land
and are usually composed of sand, mud or pebbles. From
a recreational viewpoint, sand beaches should be clear and
healthy. Especially in higher latitudes, a significant
percentage of time is spent on the beach itself rather than
in the water. Microorganisms are a significant component
of the polluted beach sand. Bacteria, fungi, parasites and
viruses have all been isolated from polluted beach sand. A
number of genera and species that may be encountered
through contact with sand are potential pathogens.
Accordingly, concern has been expressed that beach sand
or similar materials may act as reservoirs or vectors of
infection (Nestor et al., 1984; Roses et al., 1988; Mendes
et al., 1997) although transmission by this route has not
been demonstrated in epidemiological studies.
The prevalence of parasites in wet sand (swash zone)
may attribute to the nature of the sand which act as filter
and cumulative tool, but the prevalence of parasites in the
dry sand may be attributed to the tidal action, when
seawater cover the dry sand the parasites remain in the
dry sand.
As mentioned, wastewater effluent may transport
faecal-associated microorganisms such as parasites, which
are implicated in thousands of illnesses each year among
people who consume contaminated shellfish (Burkhardt
and Calci, 2000; Shieh et al., 2000). There is a substantial
need for better detection and identification of wastewater
influences on coastal systems to inform bathers and
fisheries management and protect public health (Randall,
2003; Savage, 2005).
The situation of random discharge points of sewage
exists since decades, only four WWTPs present in Gaza
Strip, the situation of contamination sandy beach will be
worse in case of no municipal or environmental actions
were taken.
The cycle of parasitic diseases still exist in Gaza Strip,
and could be found within infected human, some animals,
contaminated food, polluted environments and unsuitable
drinking water reservoirs. Additionally, many reported
210
© 2013Jordan Journal of Biological Sciences. All rights reserved - Volume 6, Number 3
literatures were carried out in Gaza Strip confirm the
endemicity of the human gastrointestinal parasites is in
one of the most crowded and overpopulated area in the
world. Yassin et al. (1999) reported that the prevalence of
intestinal among school children which was 27.6%.
In a study carried out by Kanoa (2006) the impact of
health education programmer's intervention on the
prevalence of intestinal parasites among school children
in Gaza city,
Beit-lahia villages and Jabalia refugee
camp "Gaza Strip" were examined for 6 months. The rule
of health education in decreasing prevalence of intestinal
parasitic infection was statistically significant (P= 0.001).
A comparison study was carried out between two regions
in Gaza strip, it was found that Prevalence of intestinal
parasites was high in Jabalia village (more than 53%) in
comparison to Rimal area 33% (Al Agha and Teodorescu,
2002). A house hold survey included 1000 individuals
from all ages was conducted in Biet-lahia, Palestine It was
found that (72.9%) of examined individuals were infected
with different types of intestinal parasites (Al-Zain and Al
Hindi, 2005).
These studies confirm the source of intestinal parasites
from decades and continue to exist from the infected hosts
to the sewers which find its way to the shoreline region.
5. Conclusion
It is concluded that the prevalence of parasites in wet
sand is higher than in adjacent dry sand, as the wet sand
behaves as a passive harbor for cumulative pollution.
6. Recommendations
It is recommended that contaminated areas should be
identified and residents should be aware of such risk
which is a result of the contamination of sandy Gaza sea
shore, and the multiple discharge point should be grouped
into one.
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