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7 Horns 7 Eyes is an American Christian progressive death metal band based out of Seattle, Washington. The band formed in 2006 and released their debut album Throes of Absolution on April 24, 2012, on Century Media Records in North America and Basick Records in Europe. On January 10, 2014, after being silent for nearly two years, Vocalist JJ Polachek announced the band is working on three potential releases for 2014 and 2015. It was described as large a conceptual work.

History

Formation and 7 Horns 7 Eyes

In 2006, friends, Kyle Wood, Brandon and Aaron Smith, Chris Weiford, and Steven Bye started a band, that they named 7 Horns 7 Eyes, which comes from Revelation 5:6 in the Bible. They recorded their self titled debut EP in 2006 and released it in 2007. After 7 Horns 7 Eyes was released, Weiford and Bye left the band and were replaced by friend Sean Alf, and Ryan Wood, Kyle's brother.

"Huntour", Convalescence EP, and Throes of Absolution

In 2009, the band was selected for Demon Hunter's first annual "Huntour" along with the resurrected Living Sacrifice, The Famine, Focused, and Advent. After the "Huntour" the band began to record their first album and released the first single of the album, "The Vindicator". After recording the whole album, Kyle Wood left to get married. Aaron Smith then contacted his friend JJ "Shiv" Polachek of Monotheist, to be the new vocalist and re-record vocals. In 2011, the band released Convalescence EP and in 2012, their debut album, Throes of Absolution was released. After the release, Sean Alf left the band, and was replaced by Zack Uidl.

Hematoxylin and eosin stain or haematoxylin and eosin stain (H&E stain or HE stain) is one of the principal stains in histology. It is the most widely used stain in medical diagnosis and is often the gold standard; for example when a pathologist looks at a biopsy of a suspected cancer, the histological section is likely to be stained with H&E and termed "H&E section", "H+E section", or "HE section". A combination of hematoxylin and eosin, it produces blues, violets, and reds.

Principle

The staining method involves application of hemalum, a complex formed from aluminium ions and hematein (an oxidation product of hematoxylin). Hemalum colors nuclei of cells (and a few other objects, such as keratohyalin granules and calcified material) blue. The nuclear staining is followed by counterstaining with an aqueous or alcoholic solution of eosin Y, which colors eosinophilic structures in various shades of red, pink and orange.

The staining of nuclei by hemalum is ordinarily due to binding of the dye-metal complex to DNA, but nuclear staining can be obtained after extraction of DNA from tissue sections. The mechanism is different from that of nuclear staining by basic (cationic) dyes such as thionine or toluidine blue. Staining by basic dyes occurs only from solutions that are less acidic than hemalum, and it is prevented by prior chemical or enzymatic extraction of nucleic acids. There is evidence to indicate that coordinate bonds, similar to those that hold aluminium and hematein together, bind the hemalum complex to DNA and to carboxy groups of proteins in the nuclear chromatin.