Les plantes sont constamment exposees aux stress environnementaux qui perturbent leur fonctionnem... more Les plantes sont constamment exposees aux stress environnementaux qui perturbent leur fonctionnement. Parmi les stress abiotiques, le deficit hydrique limite les rendements plus severement que tout autre facteur environnemental. Le deficit hydrique est principalement cause par deux types de stress abiotiques : la secheresse et la salinite. Les effets de differents niveaux de stress hydrique et de NaCl sur les parametres oxydatifs (H2O2 et MDA), l'ascorbate, les activites des enzymes antioxydants : la superoxyde dismutase (SOD) et la catalase (CAT), ainsi que les activites et les niveaux d'accumulation relatifs des transcrits de genes codant pour les enzymes de cycle ascorbate-glutathion : l'ascorbate peroxidate (APX), le monodehydroascorbate reductase (MDHAR), la dehydroascorbate reductase (DHAR) et la glutathion reductase (GR), ont ete etudies dans les fruits de tomate (Solanum lycopersicum L. Cv. Micro-Tom). Les changements des contenus en H2O2 et MDA indiquent que les...
This study was conducted at the Biotechnology Department, Swaida Research Center, GCSAR, and Syri... more This study was conducted at the Biotechnology Department, Swaida Research Center, GCSAR, and Syria during 2016. Eight wheat genotypes were screened at the seedling stage using Sorbitol to mimic drought in the laboratory. The experiment was performed using Complete Randomized Design and the genotypes were evaluated by Cluster analysis based on the sum of reduction ratios for shoot and root length. The results revealed variability in the experimented genotypes' sensitivity towards water stress in shoot and root length. Cham 10 showed the highest values whereas Doma 1 recorded the lowest. These results referred to the existence of genetic variation among genotypes under study. In addition, Cluster analysis indicated classifying studied genotypes into two groups: tolerant group containing Cham 10 (the most tolerant genotype), Bohuth 11, Cham 3, Bohuth 7, and sensitive group containing Doma 1 (the most sensitive genotype), Jolan 2, Doma 4 and Bohuth 8.
Journal of Agricultural Science and Technology, 2015
Salt stress negatively impacts crops yield throughout the world. Nine varieties of potato (Solanu... more Salt stress negatively impacts crops yield throughout the world. Nine varieties of potato (Solanum tuberosum L.) were screened for salt stress tolerance by measuring in-vitro growth of the aerial plant parts, as well as roots. Salt stress was evaluated by adding 25, 50, 75, 100, 125, 150 and 200 mM of NaCl to Murashige- Skoog (MS) medium and compared to MS medium without NaCl. Plant length and stem thickness, leaf area, roots number, length, and thickness, and plant fresh and dry weights were measured. Osmotic pressure ( �medium , MPa) and electrical conductivity (EC medium , mS cm -1 ) of media ranged from �0.2 to �0.91 MPa and 5.8 to 24 mS cm -1 , respectively. Salt stress adversely affected the plant growth, and varieties differed in their responses. Progressive reduction in the studied parameters occurred as NaCl levels increased. Grouping all the varieties by cluster analysis, based on the growth parameters response to salt stress, resulted in three distinct groups: (1) salt to...
The experiment was carried in 2012/2013 season to study the effect of foliar spraying treatments ... more The experiment was carried in 2012/2013 season to study the effect of foliar spraying treatments with liquorice (Glycyrrihiza glabra) root extract (5 and 10 g/l) and GA3 (50 and 100 ppm), in comparison with distilled water, on growth of Baladi onion at three different levels of irrigation (100, 75, and 50% of field capacity). Onion bulblets were planted in pots and spraying started when plants had 3-4 leaves and repeated every two weeks until plants complete their vegetative growth. Water stress period of 20 days was applied when plants had 6-7 leaves. The results showed that the treatment with liquorice extract and gibberellic acid led to a significant increase in all indicators studied. Reducing the level of irrigation resulted in a significant decrease in plant height, leaf content of chlorophyll, and bulb weight, versus an increase in the concentration of proline, and the percentage of dry matter, and total soluble solids. However, the interaction between foliar spraying and irr...
This study was carried out during the period of 2017-2018 aiming to study genetic relationship fo... more This study was carried out during the period of 2017-2018 aiming to study genetic relationship for seven olive cultivars grown in the Coastal Area of Syria. We used 21 pairs of Simple Sequence Repeats (SSR) markers. The total markers produced 58 alleles with an average of 2.76 allele per locus, number of alleles generated SSR markers, and the number of SSR markers per primer pair ranged from 1 to (UDO99-027 -UDO99-026- DCA18), 6 alleles (for one marker DCA3). Polymorphic information content (PIC) values ranged from 0.2149 (UDO99-027-UDO99-031) to 0.7913 DCA3 with an average 0.3907. Genetic diversity (GD) ranged from 0.103 (UDO99-031) to 0.755(UDO99-008) with an average 0.488. The results of cluster analysis and dendrogram discriminated all genotypes and clustered them separately into three major groups. Group I: Dab1, group II: KHODEIRI, DOEBLI, Dab 2, SUKARY, AYRONY, groups III: FRONTOYO. The results confirmed the ability of SSR markers to be used for the determination of genetic d...
Jordan Journal of Agricultural Sciences, Jun 17, 2014
Changes in the ascorbate-glutathione cycle components were measured during tomato fruit developme... more Changes in the ascorbate-glutathione cycle components were measured during tomato fruit development. The activities of ascorbate-glutathione cycle enzymes were modified with fruit development. The activities of ascorbate peroxidase enzyme (APX) and dehydroascorbate reductase (DHAR) increased during fruit growth and ripening. However, the activities of monodehydroascorbate reductase enzyme (MDHAR) and glutathione reductase enzyme (GR) increased only during fruit ripening. Changes were also noted in the fruit total ascorbate (AsA plus DHA), ascorbate (AsA) and dehydroascorbate (DHA) contents during fruit development. These changes in the ascorbate-glutathione cycle components, enzymatic and non enzymatic, establish that this cycle plays an important role during fruit development stages.
Les plantes sont constamment exposees aux stress environnementaux qui perturbent leur fonctionnem... more Les plantes sont constamment exposees aux stress environnementaux qui perturbent leur fonctionnement. Parmi les stress abiotiques, le deficit hydrique limite les rendements plus severement que tout autre facteur environnemental. Le deficit hydrique est principalement cause par deux types de stress abiotiques : la secheresse et la salinite. Les effets de differents niveaux de stress hydrique et de NaCl sur les parametres oxydatifs (H2O2 et MDA), l'ascorbate, les activites des enzymes antioxydants : la superoxyde dismutase (SOD) et la catalase (CAT), ainsi que les activites et les niveaux d'accumulation relatifs des transcrits de genes codant pour les enzymes de cycle ascorbate-glutathion : l'ascorbate peroxidate (APX), le monodehydroascorbate reductase (MDHAR), la dehydroascorbate reductase (DHAR) et la glutathion reductase (GR), ont ete etudies dans les fruits de tomate (Solanum lycopersicum L. Cv. Micro-Tom). Les changements des contenus en H2O2 et MDA indiquent que les differents stress ont induit un stress oxydatif dans les fruits. Les concentrations d'ascorbate (AsA) et de dehydroascorbate (DHA), ont generalement change avec le stress. En outre, les activites de la SOD de la CAT et les activites et les niveaux d'accumulation relatifs des transcrits de la DHAR, la MDHAR, l'APX et la GR sont modifies par le stade de developpement du fruit et l'intensite et la duree de stress. Ces resultats suggerent que la reponse des systemes antioxydants des fruits de tomate au stress oxydatif, est differente selon le stade du developpement du fruit. L'utilisation de mercure pour provoquer un deficit hydrique rapide met en evidence l'existence d'un signal provenant des feuilles et controlant la regulation des enzymes du cycle ascorbate-glatathion. Les resultats obtenus sur des fruits detaches suggerent que l'H2O2 et NO sont fortement impliques dans cette signalisation
In this study, the differential behaviour of antioxidant systems between water stress and salt st... more In this study, the differential behaviour of antioxidant systems between water stress and salt stress, considering two stages of development of the fruit was investigated. The activities of superoxide dismutase (SOD) and catalase (CAT), as well as the activities and the relative transcript levels of the enzymes of ascorbate-glutathione cycle: ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR), the levels of ascorbate pool and hydrogen peroxide (H 2 O 2) and malonyldialdehyde (MDA) contents were studied in fruits of tomato plants (Solanum lycopersicum L. cv. 'Micro-Tom') subjected to water and salt stresses. Water deficit was induced by holding off the irrigation for 3 and 6 days. To apply salt stress, plants were treated with 100 mM of NaCl for 3 and 6 days. H 2 O 2 content was generally increased with both water and salt stresses, however, MDA content was increased only with salt stress. Chang...
Physiology and molecular biology of plants : an international journal of functional plant biology, 2013
The effects of different levels of water stress on oxidative parameters (H2O2 and MDA), the total... more The effects of different levels of water stress on oxidative parameters (H2O2 and MDA), the total pool sizes of ascorbate, the activities of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), as well as the activities and relative transcript levels of the enzymes of ascorbate-glutathione cycle ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR) and glutathione reductase (GR) were studied in the fruit of tomato (Solanum lycopersicum L. cv. Micro-Tom). Plants were subjected to three levels of water stress (S50, S25 and S0) and fruits at different development stages were harvested after 3, 6 and 10 days of stress. Changes in H2O2 and MDA contents indicated that water stress induced oxidative stress in fruits. The concentrations of ascorbate (AsA) and dehydroascorbate (DHA) generally modified with water stress treatments. Moreover, changes in SOD and CAT activities and DHAR, MDHAR, APX and GR activities and relative trans...
Physiology and molecular biology of plants : an international journal of functional plant biology, 2012
The study was carried out to determine the responses of two parsley (Petroselinum crispum L.) hyb... more The study was carried out to determine the responses of two parsley (Petroselinum crispum L.) hybrids; plain and curly- leafed to water stress. Besides the control, three irrigation treatments (10, 30 and 50 % of control water amount) were applied. Plant yield (leaf area, stem length and diameter, number of leaves) and quality (fresh and dry weights and concentrations of chlorophyll, carotenes, anthocyanins and vitamin C) were measured. The responses of plant yield and quality varied according to the hybrid and water treatment. The high quality was observed in curly- leafed parsley if compared to plain- leafed parsley, which had higher yield (stem length and diameter, leaf area). Moreover, water stress improved quality but decreased yield parameters in both hybrids.
Here, we describe microplate assays for determining the specific activities of four enzymes that ... more Here, we describe microplate assays for determining the specific activities of four enzymes that constitute the ascorbate–glutathione cycle: APX, MDHAR, DHAR, and GR. In plants, these enzymes play a major role in detoxifying reactive oxygen species produced in cells under environmental stress. This work presents the development of plate reader assays to allow rapid analysis of the ascorbate–glutathione cycle activity using tomato fruits subjected to salt stress as a model. With this method, it is possible to analyze easily in one day the activities of the four enzymes for 30 experimental samples, all in triplicate and with blanks.
Les plantes sont constamment exposees aux stress environnementaux qui perturbent leur fonctionnem... more Les plantes sont constamment exposees aux stress environnementaux qui perturbent leur fonctionnement. Parmi les stress abiotiques, le deficit hydrique limite les rendements plus severement que tout autre facteur environnemental. Le deficit hydrique est principalement cause par deux types de stress abiotiques : la secheresse et la salinite. Les effets de differents niveaux de stress hydrique et de NaCl sur les parametres oxydatifs (H2O2 et MDA), l'ascorbate, les activites des enzymes antioxydants : la superoxyde dismutase (SOD) et la catalase (CAT), ainsi que les activites et les niveaux d'accumulation relatifs des transcrits de genes codant pour les enzymes de cycle ascorbate-glutathion : l'ascorbate peroxidate (APX), le monodehydroascorbate reductase (MDHAR), la dehydroascorbate reductase (DHAR) et la glutathion reductase (GR), ont ete etudies dans les fruits de tomate (Solanum lycopersicum L. Cv. Micro-Tom). Les changements des contenus en H2O2 et MDA indiquent que les...
This study was conducted at the Biotechnology Department, Swaida Research Center, GCSAR, and Syri... more This study was conducted at the Biotechnology Department, Swaida Research Center, GCSAR, and Syria during 2016. Eight wheat genotypes were screened at the seedling stage using Sorbitol to mimic drought in the laboratory. The experiment was performed using Complete Randomized Design and the genotypes were evaluated by Cluster analysis based on the sum of reduction ratios for shoot and root length. The results revealed variability in the experimented genotypes' sensitivity towards water stress in shoot and root length. Cham 10 showed the highest values whereas Doma 1 recorded the lowest. These results referred to the existence of genetic variation among genotypes under study. In addition, Cluster analysis indicated classifying studied genotypes into two groups: tolerant group containing Cham 10 (the most tolerant genotype), Bohuth 11, Cham 3, Bohuth 7, and sensitive group containing Doma 1 (the most sensitive genotype), Jolan 2, Doma 4 and Bohuth 8.
Journal of Agricultural Science and Technology, 2015
Salt stress negatively impacts crops yield throughout the world. Nine varieties of potato (Solanu... more Salt stress negatively impacts crops yield throughout the world. Nine varieties of potato (Solanum tuberosum L.) were screened for salt stress tolerance by measuring in-vitro growth of the aerial plant parts, as well as roots. Salt stress was evaluated by adding 25, 50, 75, 100, 125, 150 and 200 mM of NaCl to Murashige- Skoog (MS) medium and compared to MS medium without NaCl. Plant length and stem thickness, leaf area, roots number, length, and thickness, and plant fresh and dry weights were measured. Osmotic pressure ( �medium , MPa) and electrical conductivity (EC medium , mS cm -1 ) of media ranged from �0.2 to �0.91 MPa and 5.8 to 24 mS cm -1 , respectively. Salt stress adversely affected the plant growth, and varieties differed in their responses. Progressive reduction in the studied parameters occurred as NaCl levels increased. Grouping all the varieties by cluster analysis, based on the growth parameters response to salt stress, resulted in three distinct groups: (1) salt to...
The experiment was carried in 2012/2013 season to study the effect of foliar spraying treatments ... more The experiment was carried in 2012/2013 season to study the effect of foliar spraying treatments with liquorice (Glycyrrihiza glabra) root extract (5 and 10 g/l) and GA3 (50 and 100 ppm), in comparison with distilled water, on growth of Baladi onion at three different levels of irrigation (100, 75, and 50% of field capacity). Onion bulblets were planted in pots and spraying started when plants had 3-4 leaves and repeated every two weeks until plants complete their vegetative growth. Water stress period of 20 days was applied when plants had 6-7 leaves. The results showed that the treatment with liquorice extract and gibberellic acid led to a significant increase in all indicators studied. Reducing the level of irrigation resulted in a significant decrease in plant height, leaf content of chlorophyll, and bulb weight, versus an increase in the concentration of proline, and the percentage of dry matter, and total soluble solids. However, the interaction between foliar spraying and irr...
This study was carried out during the period of 2017-2018 aiming to study genetic relationship fo... more This study was carried out during the period of 2017-2018 aiming to study genetic relationship for seven olive cultivars grown in the Coastal Area of Syria. We used 21 pairs of Simple Sequence Repeats (SSR) markers. The total markers produced 58 alleles with an average of 2.76 allele per locus, number of alleles generated SSR markers, and the number of SSR markers per primer pair ranged from 1 to (UDO99-027 -UDO99-026- DCA18), 6 alleles (for one marker DCA3). Polymorphic information content (PIC) values ranged from 0.2149 (UDO99-027-UDO99-031) to 0.7913 DCA3 with an average 0.3907. Genetic diversity (GD) ranged from 0.103 (UDO99-031) to 0.755(UDO99-008) with an average 0.488. The results of cluster analysis and dendrogram discriminated all genotypes and clustered them separately into three major groups. Group I: Dab1, group II: KHODEIRI, DOEBLI, Dab 2, SUKARY, AYRONY, groups III: FRONTOYO. The results confirmed the ability of SSR markers to be used for the determination of genetic d...
Jordan Journal of Agricultural Sciences, Jun 17, 2014
Changes in the ascorbate-glutathione cycle components were measured during tomato fruit developme... more Changes in the ascorbate-glutathione cycle components were measured during tomato fruit development. The activities of ascorbate-glutathione cycle enzymes were modified with fruit development. The activities of ascorbate peroxidase enzyme (APX) and dehydroascorbate reductase (DHAR) increased during fruit growth and ripening. However, the activities of monodehydroascorbate reductase enzyme (MDHAR) and glutathione reductase enzyme (GR) increased only during fruit ripening. Changes were also noted in the fruit total ascorbate (AsA plus DHA), ascorbate (AsA) and dehydroascorbate (DHA) contents during fruit development. These changes in the ascorbate-glutathione cycle components, enzymatic and non enzymatic, establish that this cycle plays an important role during fruit development stages.
Les plantes sont constamment exposees aux stress environnementaux qui perturbent leur fonctionnem... more Les plantes sont constamment exposees aux stress environnementaux qui perturbent leur fonctionnement. Parmi les stress abiotiques, le deficit hydrique limite les rendements plus severement que tout autre facteur environnemental. Le deficit hydrique est principalement cause par deux types de stress abiotiques : la secheresse et la salinite. Les effets de differents niveaux de stress hydrique et de NaCl sur les parametres oxydatifs (H2O2 et MDA), l'ascorbate, les activites des enzymes antioxydants : la superoxyde dismutase (SOD) et la catalase (CAT), ainsi que les activites et les niveaux d'accumulation relatifs des transcrits de genes codant pour les enzymes de cycle ascorbate-glutathion : l'ascorbate peroxidate (APX), le monodehydroascorbate reductase (MDHAR), la dehydroascorbate reductase (DHAR) et la glutathion reductase (GR), ont ete etudies dans les fruits de tomate (Solanum lycopersicum L. Cv. Micro-Tom). Les changements des contenus en H2O2 et MDA indiquent que les differents stress ont induit un stress oxydatif dans les fruits. Les concentrations d'ascorbate (AsA) et de dehydroascorbate (DHA), ont generalement change avec le stress. En outre, les activites de la SOD de la CAT et les activites et les niveaux d'accumulation relatifs des transcrits de la DHAR, la MDHAR, l'APX et la GR sont modifies par le stade de developpement du fruit et l'intensite et la duree de stress. Ces resultats suggerent que la reponse des systemes antioxydants des fruits de tomate au stress oxydatif, est differente selon le stade du developpement du fruit. L'utilisation de mercure pour provoquer un deficit hydrique rapide met en evidence l'existence d'un signal provenant des feuilles et controlant la regulation des enzymes du cycle ascorbate-glatathion. Les resultats obtenus sur des fruits detaches suggerent que l'H2O2 et NO sont fortement impliques dans cette signalisation
In this study, the differential behaviour of antioxidant systems between water stress and salt st... more In this study, the differential behaviour of antioxidant systems between water stress and salt stress, considering two stages of development of the fruit was investigated. The activities of superoxide dismutase (SOD) and catalase (CAT), as well as the activities and the relative transcript levels of the enzymes of ascorbate-glutathione cycle: ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR), the levels of ascorbate pool and hydrogen peroxide (H 2 O 2) and malonyldialdehyde (MDA) contents were studied in fruits of tomato plants (Solanum lycopersicum L. cv. 'Micro-Tom') subjected to water and salt stresses. Water deficit was induced by holding off the irrigation for 3 and 6 days. To apply salt stress, plants were treated with 100 mM of NaCl for 3 and 6 days. H 2 O 2 content was generally increased with both water and salt stresses, however, MDA content was increased only with salt stress. Chang...
Physiology and molecular biology of plants : an international journal of functional plant biology, 2013
The effects of different levels of water stress on oxidative parameters (H2O2 and MDA), the total... more The effects of different levels of water stress on oxidative parameters (H2O2 and MDA), the total pool sizes of ascorbate, the activities of antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT), as well as the activities and relative transcript levels of the enzymes of ascorbate-glutathione cycle ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR) and glutathione reductase (GR) were studied in the fruit of tomato (Solanum lycopersicum L. cv. Micro-Tom). Plants were subjected to three levels of water stress (S50, S25 and S0) and fruits at different development stages were harvested after 3, 6 and 10 days of stress. Changes in H2O2 and MDA contents indicated that water stress induced oxidative stress in fruits. The concentrations of ascorbate (AsA) and dehydroascorbate (DHA) generally modified with water stress treatments. Moreover, changes in SOD and CAT activities and DHAR, MDHAR, APX and GR activities and relative trans...
Physiology and molecular biology of plants : an international journal of functional plant biology, 2012
The study was carried out to determine the responses of two parsley (Petroselinum crispum L.) hyb... more The study was carried out to determine the responses of two parsley (Petroselinum crispum L.) hybrids; plain and curly- leafed to water stress. Besides the control, three irrigation treatments (10, 30 and 50 % of control water amount) were applied. Plant yield (leaf area, stem length and diameter, number of leaves) and quality (fresh and dry weights and concentrations of chlorophyll, carotenes, anthocyanins and vitamin C) were measured. The responses of plant yield and quality varied according to the hybrid and water treatment. The high quality was observed in curly- leafed parsley if compared to plain- leafed parsley, which had higher yield (stem length and diameter, leaf area). Moreover, water stress improved quality but decreased yield parameters in both hybrids.
Here, we describe microplate assays for determining the specific activities of four enzymes that ... more Here, we describe microplate assays for determining the specific activities of four enzymes that constitute the ascorbate–glutathione cycle: APX, MDHAR, DHAR, and GR. In plants, these enzymes play a major role in detoxifying reactive oxygen species produced in cells under environmental stress. This work presents the development of plate reader assays to allow rapid analysis of the ascorbate–glutathione cycle activity using tomato fruits subjected to salt stress as a model. With this method, it is possible to analyze easily in one day the activities of the four enzymes for 30 experimental samples, all in triplicate and with blanks.
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