Papers by Ioana Berindan Neagoe
Nutrients, 2021
Nutrition plays an important role in overall human health. Although there is no direct evidence s... more Nutrition plays an important role in overall human health. Although there is no direct evidence supporting the direct involvement of nutrition in curing disease, for some diseases, good nutrition contributes to disease prevention and our overall well-being, including energy level, optimum internal function, and strength of the immune system. Lately, other major, but more silent players are reported to participate in the body’s response to ingested nutrients, as they are involved in different physiological and pathological processes. Furthermore, the genetic profile of an individual is highly critical in regulating these processes and their interactions. In particular, miR-155, a non-coding microRNA, is reported to be highly correlated with such nutritional processes. In fact, miR-155 is involved in the orchestration of various biological processes such as cellular signaling, immune regulation, metabolism, nutritional responses, inflammation, and carcinogenesis. Thus, this review aim...
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Symmetry, 2021
Infections can be triggered by a wide range of pathogens. However, there are few strains of bacte... more Infections can be triggered by a wide range of pathogens. However, there are few strains of bacteria that cause illness, but some are quite life-threatening. Likewise, viral infections are responsible for many human diseases, usually characterized by high contagiousness. Hence, as bacterial and viral infections can both cause similar symptoms, it can be difficult to determine the exact cause of a specific infection, and this limitation is critical. However, recent scientific advances have geared us up with the proper tools required for better diagnoses. Recent discoveries have confirmed the involvement of non-coding RNAs (ncRNAs) in regulating the pathogenesis of certain bacterial or viral infections. Moreover, the presence of reactive oxygen species (ROS) is also known as a common infection trait that can be used to achieve a more complete description of such pathogen-driven conditions. Thus, this opens further research opportunities, allowing scientists to explore infection-associ...
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Summary In our study we investigated the contribution of ox aliplatin to mediated toxicity and tr... more Summary In our study we investigated the contribution of ox aliplatin to mediated toxicity and transcription profile of genes involves in cancer i n order to understand the mechanisms involved in tumor cell apoptosis and cancer disease progression. As colorectal cancer model we used the Colo320 cell line. We evaluated t he oxaliplatin induced toxicity of a single dose of different concentrations at 24, 48 a nd 72 hours, and also multiple doses at different times, in order to establish the IC 50 concentration. p53, NF B and PDGF gene expression levels were evaluated after treatment wi th a concentration close to the IC 50 using the RT-PCR technique. The purpose of the study is to observe the changes in pharmacokinetic parameters after administration of a single dose in order to establish IC 50 , using the MTT cell proliferation assay on the lin e Colo320. We compared the parameters of cell proliferation observed after adm inistration of a single dose and multiple doses, and tried to...
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Journal of Gastrointestinal and Liver Diseases, 2020
Background and Aims: Although colon cancer has a decreasing incidence trend in Europe, because of... more Background and Aims: Although colon cancer has a decreasing incidence trend in Europe, because of its still high frequency and not fully understood pathogenesis, this malignancy still remains a subject of intense research. The aim of this study was to investigate the role of microRNA-194 and microRNA-1228 in colon cancer proliferation. Methods: RNA was extracted from patients with colon cancer with or without advanced disease and microRNA expression levels were determined through qRT-PCR. Assays were performed on HCT116 cell line and included qRT-PCR, western blotting and cell counting. Results: We observed that both microRNAs 194 and 1228 were altered in patients with colon cancer compared with healthy individuals. We observed a lower expression of both microRNA-194 and microRNA-1228 in patients with advanced colon cancer. To validate their pathogenetic role we performed viability and invasion assays on HCT116 cell line transfected with mimics or inhibitors of the mentioned microRN...
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Pharmaceutics, 2020
Cancer-associated adipocytes have functional roles in tumor development through secreted adipocyt... more Cancer-associated adipocytes have functional roles in tumor development through secreted adipocyte-derived factors and exosomes and also through metabolic symbiosis, where the malignant cells take up the lactate, fatty acids and glutamine produced by the neighboring adipocytes. Recent research has demonstrated the value of adipocytes as cell-based delivery platforms for drugs (or prodrugs), nucleic acids or loaded nanoparticles for cancer therapy. This strategy takes advantage of the biocompatibility of the delivery system, its ability to locate the tumor site and also the predisposition of cancer cells to come in functional contact with the adipocytes from the tumor microenvironment for metabolic sustenance. Also, their exosomal content can be used in the context of cancer stem cell reprogramming or as a delivery vehicle for different cargos, like non-coding nucleic acids. Moreover, the process of adipocytes isolation, processing and charging is quite straightforward, with minimal ...
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Frontiers in Immunology, 2019
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Medicina, 2019
Regarding cancer as a genetic multi-factorial disease, a number of aspects need to be investigate... more Regarding cancer as a genetic multi-factorial disease, a number of aspects need to be investigated and analyzed in terms of cancer’s predisposition, development and prognosis. One of these multi-dimensional factors, which has gained increased attention in the oncological field due to its unelucidated role in risk assessment for cancer, is diet. Moreover, as studies advance, a clearer connection between diet and the molecular alteration of patients is becoming identifiable and quantifiable, thereby replacing the old general view associating specific phenotypical changes with the differential intake of nutrients. Respectively, there are two major fields concentrated on the interrelation between genome and diet: nutrigenetics and nutrigenomics. Nutrigenetics studies the effects of nutrition at the gene level, whereas nutrigenomics studies the effect of nutrients on genome and transcriptome patterns. By precisely evaluating the interaction between the genomic profile of patients and the...
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Scientific reports, Jan 6, 2017
Saponins from defatted root-extract of Securidaca longipedunculata were systematically entrapped ... more Saponins from defatted root-extract of Securidaca longipedunculata were systematically entrapped in emulsion monolayer-barrier and finally recovered in pure form through demulsification. First, their molecules were dispersed in water to engineer a monomolecular film architecture, via self-assembly. Emulsifying with ethyl-ether resulted in swollen micelles and engendered phase-inversion and phase-separation, by disrupting the thermodynamic equilibrium. As positive outcome, a Winsor II system was obtained, having saponin-rich upper phase (ethyl-ether) and impurities bound lower phase (aqueous). Saponin particles underwent transition in insoluble ethyl-ether, precipitated and recovered as solids. The entire process was bioactivity-guided and validated using pooled fractions of securidaca saponins, purified by TLC (RP-C18, FS). TEM and SEM revealed interesting morphologies and particle sizes between nanometer and micron. At the end, purity output of 90% and total recovery of 94% were ac...
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Critical Reviews™ in Oncogenesis, 2018
Although numerous bibliometric studies have examined various aspects of cancer research, the land... more Although numerous bibliometric studies have examined various aspects of cancer research, the landscape of scientific studies focusing on natural products in cancer research has not been characterized. Using the Web of Science Core Collection online database, we identify and analyze scientific articles on natural products in cancer-related research. English is the language of publication for 99% of articles. In general, annual citation count of an article increases quickly after publication, reaches a plateau in the second year, stays in this plateau for 10 yr, and then begins to fall. The five most contributing journal categories are medicinal chemistry, contributing the most at 1890 articles (24% of 8012 articles); oncology (20%, with 1572 articles); pharmacology and pharmacy (19%, with 1557); biochemistry and molecular biology (15%, with 1225); and plant sciences (11%, with 883 articles). The United States and Spain yield a larger number of articles with high average citations, and China has been increasing since 2009. Apoptosis and cytotoxicity are the two most-frequently used keywords. Effects of natural products on autophagy with a relevance to cancer are mentioned in 69 publications. Our literature analysis reveals a dynamically evolving landscape and an increasing volume of research investigations that are focused on the study of natural products in the context of cancer. Curcumin, flavonoids, and resveratrol are the most-frequently mentioned natural products. Cancer of the breast, prostate, and colon are the most-frequently mentioned cancers.
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Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, Jan 18, 2018
Phytol (PYT) is a diterpene member of the long-chain unsaturated acyclic alcohols. PYT and some o... more Phytol (PYT) is a diterpene member of the long-chain unsaturated acyclic alcohols. PYT and some of its derivatives, including phytanic acid (PA), exert a wide range of biological effects. PYT is a valuable essential oil (EO) used as a fragrance and a potential candidate for a broad range of applications in the pharmaceutical and biotechnological industry. There is ample evidence that PA may play a crucial role in the development of pathophysiological states. Focusing on PYT and some of its most relevant derivatives, here we present a systematic review of reported biological activities, along with their underlying mechanism of action. Recent investigations with PYT demonstrated anxiolytic, metabolism-modulating, cytotoxic, antioxidant, autophagy- and apoptosis-inducing, antinociceptive, anti-inflammatory, immune-modulating, and antimicrobial effects. PPARs- and NF-κB-mediated activities are also discussed as mechanisms responsible for some of the bioactivities of PYT. The overall goa...
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Romanian Journal Of Internal Medicine, 2016
In the March 2016 issue of the Lancet Haematology, the editorial office published a paper stating... more In the March 2016 issue of the Lancet Haematology, the editorial office published a paper stating the roadmap for European research in hematology, based on the European Hematology Association (EHA) consensus document that outlines the directions in hematology for the following years across the continent. The meeting entitled “Insights in hematology” is organized a support for the initiative of a roadmap for European hematologists regarding research, may it be basic research or clinical research, but this consensus should not be focused mainly on European institutions, but rather form the backbone of global research between Europe and the United States, Japan or any other country. This will allow Europeans to learn as well as to share their experience with the rest of the scientific and medical community. And the Cluj-Napoca meeting should be followed by other such meetings all across the EU.
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Mediators of Inflammation, 2016
Tumors act systemically to sustain cancer progression, affecting the physiological processes in t... more Tumors act systemically to sustain cancer progression, affecting the physiological processes in the host and triggering responses in the blood circulating cells. In this study, we explored blood transcriptional patterns of patients with two subtypes of HER2 negative breast cancers, with different prognosis and therapeutic outcome. Peripheral blood samples from seven healthy female donors and 29 women with breast cancer including 14 triple-negative breast cancers and 15 hormone-dependent breast cancers were evaluated by microarray. We also evaluated the stroma in primary tumors. Transcriptional analysis revealed distinct molecular signatures in the blood of HER2− breast cancer patients according to ER/PR status. Our data showed the implication of immune signaling in both breast cancer subtypes with an enrichment of these processes in the blood of TNBC patients. We observed a significant alteration of “chemokine signaling,” “IL-8 signaling,” and “communication between innate and adapt...
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Medicine and Pharmacy Reports, 2016
MicroRNAs or miRNAs are small non-coding RNAs that regulate gene expression. Their discovery has ... more MicroRNAs or miRNAs are small non-coding RNAs that regulate gene expression. Their discovery has brought new knowledge in biological processes of cancer. Involvement of miRNAs in cancer development includes several major pathways from cell transformation to tumor cell development, metastasis and resistance to treatment. The first part of this review discusses miRNAs function in the intrinsic and extrinsic pathways of apoptosis. Due to the fact that many miRNAs that regulate apoptosis have been shown to play a major role in tumor cell resistance to treatment, in the second part of the review we aim at discussing miRNAs potential in becoming curative molecules.
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PLOS ONE, 2015
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PLoS ONE, 2014
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European Cytokine Network, 2012
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Cancer Biomarkers, 2014
Glioblastoma multiforme (GBM) represents a very aggressive brain tumor. Angiogenesis is the forma... more Glioblastoma multiforme (GBM) represents a very aggressive brain tumor. Angiogenesis is the formation of a network of new blood vessels, from preexisting ones. It plays an important role in the formation of the tumor, as it supplies it with oxygen and nutrients. Angiogenesis and inflammation play essential roles in glioblastoma development. These processes are regulated by the balance of a few molecules, acting as pro- or antiangiogenic and pro- or anti-inflammatory factors. The purpose of our study was to evaluate the expression of 7 markers involved in angiogenesis and inflammation pathways in patients with glioblastoma. VEGF, PDGF-bb, IGF-1, TGF-β, TNF-α, IL-6 and IL-8 levels were measured using the ELISA method, in the preoperative sera of 14 patients with histopathologically confirmed glioblastoma multiforme and 32 healthy patients. Serum levels of PDGF-bb, IGF-1 and IL-8 were significantly higher in patients with GBM, compared to the control group (p-value < 0.01). A statistically significant correlation has been found between IGF-1 and IL-6 levels (rho= -0.53, p-value < 0.05) and also between TNF-α and IL-6 levels (rho=0.60, p-value < 0.05). Statistically significant associations have been found between the presence of low levels of IL-8 and the development of coagulation necrosis (p-value < 0.05), high levels of VEGF and development of ischemic necrosis (p-value < 0.01) and high levels of IL-8 and the development of endothelial hyperplasia (p-value < 0.05). We have observed no statistically significant associations between the serum levels of the markers and the survival rates.
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International Journal of Molecular Sciences, 2014
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Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2013
ABSTRACT The utility of polyethylene glycol (PEG) modified gold nanoparticles (GNPs) as novel dru... more ABSTRACT The utility of polyethylene glycol (PEG) modified gold nanoparticles (GNPs) as novel drug carriers was comprehensively described in the last decade. We report a very effective, simple, one step and rapid synthesis method for stable, highly surface-enhanced Raman scattering (SERS) active gold colloids, by reduction and stabilization with short and long chain polyethylene glycol, PEG200 and PEG8000, respectively. Depending on the mixing rate during the synthesis, the mean size of the GNPs can be controlled between 15 and 60 nm. The obtained colloids have been characterized by UV–vis spectroscopy, transmission electron microscopy (TEM), and SERS. TEM micrographs reveal spherical nanoparticles with an average diameter of 15 nm, and polygonal particles with a mean size of 60 nm for the gold colloid with the absorption maximum at 520 nm and 562 nm, respectively. The SERS activity of these new gold colloids is comparable to that of the conventional gold colloid obtained by citrate reduction, as shown by the SERS spectra of various analytes, using the 633 laser line.
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Acta Oncologica, 2009
Apoptosis is a physiological process vital for embryologic development and the maintenance of hom... more Apoptosis is a physiological process vital for embryologic development and the maintenance of homeostasis in multicellular organisms, but it is also involved in a wide range of pathological processes, including cancer. In mammalian cells, apoptosis has been divided into two major pathways: the extrinsic pathway, activated by proapoptotic receptor signals at the cellular surface, and the intrinsic pathway, which involves the disruption of mitochondrial membrane integrity. Although many of the proteins vital for apoptosis have been identified, the molecular pathways of cellular death still remain to be elucidated. This review provides references concerning the apoptotic molecules, their interactions, the mechanisms involved in apoptosis resistance, and also the modulation of apoptosis for the treatment of cancer.
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Papers by Ioana Berindan Neagoe