Papers by Arumugam Suyavaran
Current Developments in Nutrition, 2019
Objectives We want to investigate the effects a fermented soy drink (Q-CAN® Plus) on the serum le... more Objectives We want to investigate the effects a fermented soy drink (Q-CAN® Plus) on the serum levels of inflammatory cytokines in lean and obese individuals. Methods Prospective study comprised of lean (10) and obese (10) individuals without a known diagnosis. 11 clinic visits over 14 weeks (visit 1/wk −2, visit 2/wk −1, visit 3/wk 0, visit 4/wk 1, visit 5/wk 2, visit 6/wk 3, visit 7/wk 4, visit 8/wk 6, visit 9/wk 8, visit 10/wk 10, visit 11/wk 12). Visits 1, 2 and 3 occurred prior to treatment. 237 ml of soy was dispensed at visit 3 and was consumed twice daily for 4 weeks until visit 7 (weeks 1–4). Visits 8, 9, 10 and 11 were post treatment. Blood was collected during visits 1, 3, 5, 7, 8, 9, 10,11, and serum was subjected to quantification of cytokine levels using Human Magnetic Luminex® Assays - Human XL cytokine Discovery Premixed kit (CCL-2,4,5,11,20, FLT-3 ligand, CXCL-2,3,10, PDGF AB/BB, AA, IL-1RA, IL-10, PDL-1, VEGF, GMCSF and TRAIL, expressed as pg/ml). Paired t-test and...
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Current Developments in Nutrition, 2019
Objectives In this study we want to investigate the effects of Q-CAN® Plus, the nutritional ferme... more Objectives In this study we want to investigate the effects of Q-CAN® Plus, the nutritional fermented soy drink on the hematological parameters in lean and obese individuals. Methods Prospective study comprised of lean (10) and obese (10) individuals without a known diagnosis. 11 clinic visits over 14 weeks (visit 1/wk −2, visit 2/wk −1, visit 3/wk 0, visit 4/wk 1, visit 5/wk 2, visit 6/wk 3, visit 7/wk 4, visit 8/wk 6, visit 9/wk 8, visit 10/wk 10, visit 11/wk 12). Visits 1, 2 and 3 occurred prior to treatment. 237 ml of soy was dispensed at visit 3 and was consumed twice daily for 4 weeks until visit 7 (weeks 1–4). Visits 8, 9, 10 and 11 were post treatment. Blood was collected during visits 1, 3, 5, 7, 8, 9, 10,11, and various hematological parameters were quantified at Yale New haven health, hematology lab at the Department of laboratory medicine (Yale new haven health, Yale University, CT, USA). The values were statistically analyzed using one-way ANOVA and the results were con...
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Chemico-biological interactions, Jan 22, 2017
Therapeutic applications of arsenic trioxide (ATO) are limited due to their severe adverse effect... more Therapeutic applications of arsenic trioxide (ATO) are limited due to their severe adverse effects. However, nanoparticles of ATO might possess inimitable biologic effects based on their structure and size which differ from their parent molecules. Based on this conception, AsNPs were synthesized from ATO and comparatively analysed for their interaction mechanism with DNA using spectroscopic & electrochemical techniques. Finally, anti-proliferative activity was assessed against different breast cancer cells (MDA-MB-231 & MCF-7) and normal non-cancerous cells (HEK-293). The DNA interaction study revealed that AsNPs and ATO exhibit binding constant values in the order of 106 which indicates strong binding interaction. Binding of AsNPs did not disturb the structural integrity of DNA, on the other hand an opposing effect was observed with ATO through biophysical techniques. Further, in vitro study, confirms cytotoxicity of ATO and AsNPs against different cells, however at particular conc...
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Hepatology research : the official journal of the Japan Society of Hepatology, 2017
Hepatic IR (ischemia reperfusion) injury is a commonly encountered obstacle in the post-operative... more Hepatic IR (ischemia reperfusion) injury is a commonly encountered obstacle in the post-operative management of hepatic surgery. Hepatic IR occurs during 'Pringle maneuver' for reduction of blood loss or during a brief period of cold storage followed by reperfusion of liver grafts. The stress induced during hepatic IR, triggers a spectrum of cellular responses leading to the varying degrees of hepatic complications which in turn affect the post operative care. Different preconditioning methods either activate or subdue different sets of molecular signals, resulting in varied levels of protection against hepatic IR injury. Yet, there is a serious lacuna in the knowledge regarding the choice of preconditioning methods and the resulting molecular changes in order to assess the efficiency and choice of these methods correctly. This review provides an update on the various preconditioning approaches such as surgical/ischemic, antioxidant, pharmaceutical and genetic preconditionin...
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Materials science & engineering. C, Materials for biological applications, 2017
Biomedical application of selenium nanoparticles (SeNPs) demands the eco-friendly composite for s... more Biomedical application of selenium nanoparticles (SeNPs) demands the eco-friendly composite for synthesis of SeNPs. The present study reports an aqueous extract of Allium sativum (AqEAS) plug-up the current need. Modern spectroscopic, microscopic and gravimetric techniques were employed to characterize the synthesized nanoparticles. Characterization studies revealed the formation of crystalline spherical shaped SeNPs. FTIR spectrum brings out the presence of different functional groups in AqEAS, which influence the SeNPs formation and stabilization. Furthermore the different aspects of the interaction between SeNPs and CT-DNA were scrutinized by various spectroscopic and cyclic voltametric studies. The results reveals the intercalation and groove binding mode of interaction of SeNPs with stacked base pair of CT-DNA. The Stern-Volmer quenching constant (KSV) were found to be 7.02×10(6)M-(1) (ethidium bromide), 4.22×10(6) M-(1) (acridine orange) and 7.6×10(6)M-(1) (Hoechst) indicating...
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Journal of cellular physiology, Jan 19, 2017
Troxerutin (TXER) a rutin derivative is known for its anticancer effect against hepatocellular ca... more Troxerutin (TXER) a rutin derivative is known for its anticancer effect against hepatocellular carcinoma (HCC). As part of large study, recently we have shown TXER interact with genetic material and its anti-mutagenic property. In the present study we have explored its possible mode of action in HCC. Since TXER alone did not show significant anticancer effect on Huh-7 cells, in vitro biochemical assays were performed for determining anticancer efficacy of TXER + metal complex using transition metals such as Cu, Zn & Fe. The anticancer efficacy of TXER + Cu on Huh-7 cells were evaluated using MTT assay, DCFDA, JC-1 staining, comet assay, cell cycle analysis, immunocytochemistry and western blotting. Non-toxic nature of TXER was analysed on primary rat hepatocytes. The in vivo efficacy of TXER was tested in N-nitrosodiethylamine initiated and γ-benzene hexachloride & partial hepatectomy promoted rat liver cancer. Liver markers, transition metal levels, histopathological examination an...
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Journal of Photochemistry and Photobiology B: Biology, 2015
The studies on the interaction between tRNA (transfer RNA) and small molecules are an area of rem... more The studies on the interaction between tRNA (transfer RNA) and small molecules are an area of remarkable recent attention. For this notion a fundamental knowledge of the molecular features involving the interaction of small molecules with tRNA is crucial. Hence, in the present study we have investigated the interaction of TXER (troxerutin), natural bioflavonoid rutin derivative with yeast tRNA by using various spectroscopic techniques and molecular docking studies. The UV absorption and fluorescence emission studies demonstrated external binding of TXER on tRNA with low binding constant values as compared to strong binders. Circular dichroism (CD) spectroscopy study revealed that TXER did not show any significant modification on native conformation of tRNA. Furthermore in electrochemical study, the complex of TXER-tRNA did not expose any noticeable positive potential peak shift which indicated an interaction of TXER with tRNA by electrostatic or external binding mode. The docking study showed that the hydrogen and hydrophobic interactions were involved in binding of TXER-tRNA with docking score -7.0kcal/mol. These findings led us to confirm the interaction of TXER on tRNA through external binding with low binding affinity, indicating its potential bioapplication in the future.
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RSC Advances, 2015
ZnONPs were synthesized using ferulic acid as the reductant and the intricate twofold role as DNA... more ZnONPs were synthesized using ferulic acid as the reductant and the intricate twofold role as DNA binders and radio sensitizers was revealed, which can pave the way for anticancer therapy.
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Troxerutin (TXER) is a derivative of naturally occurring bioflavonoid rutin. It possesses differe... more Troxerutin (TXER) is a derivative of naturally occurring bioflavonoid rutin. It possesses different biological activities in rising clinical world. The biological activity possessed by most of the drugs mainly targets on macromolecules. Hence, in the current study we have examined the interaction mechanism of TXER with calf thymus DNA (CT-DNA) by using various spectroscopic methods, isothermal titration calorimetry (ITC) and molecular docking studies. Further, DNA cleavage study was carried out to find the DNA protection activity of TXER. UV-absorption and emission spectroscopy showed low binding constant values via groove binding. Circular dichroism study indicates that TXER does not modify native B-form of DNA, and it retains the native B-conformation. Furthermore, no effective positive potential peak shift was observed in TXER–DNA complex during electrochemical analysis by which it represents an interaction of TXER with DNA through groove binding. Molecular docking study showed t...
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Bioorganic & Medicinal Chemistry, 2015
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Pharmaceutical Biology, 2012
Black mustard [Brassica nigra (L.) Koch] of the Brassicaceae (Cruciferae) family is commonly used... more Black mustard [Brassica nigra (L.) Koch] of the Brassicaceae (Cruciferae) family is commonly used as a spice and a cheap source of antimicrobial agents for bacterial infections. The present investigation was to demonstrate the protective effect of the methanol extract of B. nigra leaves against D-galactosamine (D-GalN)-induced hepatic and nephrotoxicity in Wistar rats. Activity of the methanol extract of B. nigra at doses of 200 and 400 mg/kg b.wt. against D-GalN (500 mg/kg b.wt.) induced toxicity, with silymarin used as the standard. Histological damage, activities of serum marker enzyme, hematological changes, metabolites such as bilirubin, urea, uric acid, and creatinine levels, tissue thiobarbutric acid reactive substance, enzymic and non-enzymic antioxidants and inflammatory marker enzymes such as myeloperoxidase, cathepsin D, and acid phosphatase were assessed. The D-GalN-induced toxicity was evident from a significant increase (p < 0.001) in the serum and tissue inflammatory markers in toxic rats, when compared with the control (saline alone treated animals). The B. nigra pretreated groups (200 and 400 mg/kg b.wt.) showed significant (p < 0.001) reduction in the D-GalN-induced toxicity as obvious from biochemical parameters. Histopathological observations confirm the protective effect of B. nigra leaf extract by reduction in hepatic and renal tissue damage. Experimentals extract showed a similar effect as the standard. The crude methanol extract of B. nigra leaf lacks inherent toxicity and exhibits hepatic and nephroprotective effects against D-GalN-induced toxicity in Wistar rats.
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Journal of Food Science, 2012
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Colloids and Surfaces B: Biointerfaces, 2013
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International Journal of Biological Macromolecules, 2012
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The bio reduction of chloro auric acid (HAuCl(4)) and silver nitrate (AgNO(3)) is achieved extrac... more The bio reduction of chloro auric acid (HAuCl(4)) and silver nitrate (AgNO(3)) is achieved extracellularly by using the aqueous extract of Solanum torvum (S. torvum) fruit. The nanoparticle formation was screened by UV-visible spectroscopy through color conversion due to surface plasma resonance bands at 560 nm and 430 nm for gold and silver nanoparticles respectively. The spherical shapes with smooth surface of gold and silver nanoparticles were analyzed through scanning electron microscope and its presence was confirmed by energy dispersive X-ray spectroscopy (SEM/EDX). The functional groups in the gold and silver salts and the bio interactive functional groups present in the S. torvum extract were characterized by employing Fourier transform infra-red spectroscopy (FTIR). The biomedical properties of gold and silver nanoparticles were premeditated as free radical scavenging activity and antibacterial static agents. Gold and silver nanoparticles serve as strong hydroxyl, superoxide, nitric oxide and DPPH radical scavengers in contrast to their corresponding metal oxides. The radical quenching properties of gold and silver nanoparticles were found to correlate with in vitro DNA protective effect. The silver nanoparticles show strong zone of inhibition against Escherichia coli, Pseudomonas and Bacillus whereas, gold nanoparticles exhibit fair zone of inhibition. To our knowledge this is the first report that S. torvum extract can reduce metal acids to nano materials.
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Inflammation Research, 2014
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Journal of Food Science and Technology, 2015
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Papers by Arumugam Suyavaran