7R7H

Peptidomimetic nitrile warheads as SARS-CoV-2 3CL protease inhibitors.

(2021) RSC Med Chem 12: 1722-1730

• PubMed: 34778773 Search on PubMedSearch on PubMed Central
• DOI: https://doi.org/10.1039/d1md00247c
• Primary Citation of Related Structures:  
  7R7H


• PubMed Abstract: 
  

  Tragically, the death toll from the COVID-19 pandemic continues to rise, and with variants being observed around the globe new therapeutics, particularly direct-acting antivirals that are easily administered, are desperately needed. Studies targeting the SARS-CoV-2 3CL protease, which is critical for viral replication, with different peptidomimetics and warheads is an active area of research for development of potential drugs. To date, however, only a few publications have evaluated the nitrile warhead as a viral 3CL protease inhibitor, with only modest activity reported. This article describes our investigation of P3 4-methoxyindole peptidomimetic analogs with select P1 and P2 groups with a nitrile warhead that are potent inhibitors of SARS-CoV-2 3CL protease and demonstrate in vitro SARS-CoV-2 antiviral activity. A selectivity for SARS-CoV-2 3CL protease over human cathepsins B, S and L was also observed with the nitrile warhead, which was superior to that with the aldehyde warhead. A co-crystal structure with SARS-CoV-2 3CL protease and a reversibility study indicate that a reversible, thioimidate adduct is formed when the catalytic sulfur forms a covalent bond with the carbon of the nitrile. This effort also identified efflux as a property limiting antiviral activity of these compounds, and together with the positive attributes described these results provide insight for further drug development of novel nitrile peptidomimetics targeting SARS-CoV-2 3CL protease.

   View More

---

Organizational Affiliation: 

Li Ka Shing Applied Virology Institute, University of Alberta Edmonton Alberta T6G 2E1 Canada jnieman@ualberta.ca.