4FTS

Crystal structure of the N363T mutant of the Flock House virus capsid

PDB DOI: https://doi.org/10.2210/pdb4FTS/pdb
Entry: 4FTS supersedes: 2Q23
NAKB: 4FTS

Classification: VIRUS
Organism(s): Flock House virus, Spodoptera frugiperda
Expression System: Spodoptera frugiperda
Mutation(s): Yes

Deposited: 2012-06-28 Released: 2012-08-01
Deposition Author(s): Speir, J.A., Chen, Z., Reddy, V.S., Johnson, J.E.

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 3.20 Å
R-Value Work: 0.259

Starting Model: experimental
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wwPDB Validation 3D Report Full Report

This is version 1.2 of the entry. See complete history.

Literature

Structural study of virus assembly intermediates reveals maturation event sequence and a staging position for externalized lytic peptides

Speir, J.A., Chen, Z., Reddy, V.S., Johnson, J.E.

To be published.

Macromolecule Content

Total Structure Weight: 136.78 kDa
Atom Count: 8,001
Modelled Residue Count: 1,001
Deposited Residue Count: 1,236
Unique protein chains: 1
Unique nucleic acid chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
Capsid protein alpha	A, B, C	407	Flock House virus	Mutation(s): 1 Gene Names: alpha, coat protein alpha EC: 3.4.23.44
UniProt
Find proteins for P12870 (Flock house virus) Explore P12870 Go to UniProtKB: P12870
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P12870
Sequence AnnotationsExpand
Reference Sequence

Find similar nucleic acids by: Sequence | 3D Structure

Entity ID: 2
Molecule	Chains	Length	Organism	Image
Random cellular RNAs	D [auth R]	15	Spodoptera frugiperda
Sequence AnnotationsExpand
Reference Sequence

Small Molecules

Ligands 4 Unique
ID	Chains	Name / Formula / InChI Key	2D Diagram	3D Interactions
EPE Query on EPE Download Ideal Coordinates CCD File SDF format, chain G [auth A] SDF format, chain J [auth B] SDF format, chain M [auth C] MOL2 format, chain G [auth A] MOL2 format, chain J [auth B] MOL2 format, chain M [auth C] mmCIF format, chain G [auth A] mmCIF format, chain J [auth B] mmCIF format, chain M [auth C]	G [auth A], J [auth B], M [auth C]	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID C₈ H₁₈ N₂ O₄ S JKMHFZQWWAIEOD-UHFFFAOYSA-N		Interactions Focus chain G [auth A] Focus chain J [auth B] Focus chain M [auth C] Interactions & Density Focus chain G [auth A] Focus chain J [auth B] Focus chain M [auth C]
SO4 Query on SO4 Download Ideal Coordinates CCD File SDF format, chain L [auth C] MOL2 format, chain L [auth C] mmCIF format, chain L [auth C]	L [auth C]	SULFATE ION O₄ S QAOWNCQODCNURD-UHFFFAOYSA-L		Interactions Focus chain L [auth C] Interactions & Density Focus chain L [auth C]
CA Query on CA Download Ideal Coordinates CCD File SDF format, chain E [auth A] SDF format, chain F [auth A] SDF format, chain H [auth B] SDF format, chain K [auth C] MOL2 format, chain E [auth A] MOL2 format, chain F [auth A] MOL2 format, chain H [auth B] MOL2 format, chain K [auth C] mmCIF format, chain E [auth A] mmCIF format, chain F [auth A] mmCIF format, chain H [auth B] mmCIF format, chain K [auth C]	E [auth A], F [auth A], H [auth B], K [auth C]	CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N		Interactions Focus chain E [auth A] Focus chain F [auth A] Focus chain H [auth B] Focus chain K [auth C] Interactions & Density Focus chain E [auth A] Focus chain F [auth A] Focus chain H [auth B] Focus chain K [auth C]
CL Query on CL Download Ideal Coordinates CCD File SDF format, chain I [auth B] MOL2 format, chain I [auth B] mmCIF format, chain I [auth B]	I [auth B]	CHLORIDE ION Cl VEXZGXHMUGYJMC-UHFFFAOYSA-M		Interactions Focus chain I [auth B] Interactions & Density Focus chain I [auth B]

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 3.20 Å
R-Value Work: 0.259
Space Group: H 3

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 326.04	α = 90
b = 326.04	β = 90
c = 770.15	γ = 120

Software Package:

Software Name	Purpose
DENZO	data reduction
SCALEPACK	data scaling
CNS	refinement
PDB_EXTRACT	data extraction
GLRF	phasing

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2012-08-01

Deposition Author(s):

This entry supersedes: 2Q23

Revision History (Full details and data files)

Version 1.0: 2012-08-01
Type: Initial release
Version 1.1: 2024-04-03
Changes: Data collection, Database references, Derived calculations, Refinement description
Version 1.2: 2024-11-06
Changes: Structure summary

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