Summary: Single nucleotide polymorphisms (SNPs) are DNA sequence variations that occur when a single nucleotide (A,T,C,or G) in the genome sequence is changed, which occur approximately once every 100 to 300 bases. There are many techniques for SNP detection and genotyping, such as restriction fragment length polymorphism PCR (RFLP-PCR), SSCP, allele specific hybridization, primer extension, allele specific oligonucleotide ligation, sequencing.
Protocols
- DMS Chemical Footprinting of RNA (Laederach Lab)
Dimethyl Sulfate is used as a chemical probe by methylation of A's and C's. Primer extension with a cy'5 labeled primer and reverse transcription allow us to determine secondary structures of RNA.
Added: Thu Dec 17 2009, Reviews: 0 Write review - Single Strand Conformation Polymorphism (SSCP) Analysis using Nondenaturing PAGE (Protocol Online)
This protocol, with brief principle and procedure will be informative and useful for molecular biology researchers working on genetic polymorphism study using SSCP technique by nondenaturing polyacrylamide gel electrophoresis (PAGE)
Added: Wed Sep 23 2009, Reviews: 0 Write review -
Single Strand Conformation Polymorphism (SSCP) analysis using Nondenaturing Polyacrylamide Gel Elect
(D.S. Kale and B.R. Yadav )
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Added: Thu Sep 17 2009, Hits: 1206, Reviews: 0 Write review