Multiple amplified microRNAs monitoring in living cells based on fluorescence quenching of Mo₂B and hybridization chain reaction

Biosens Bioelectron. 2022 Feb 1:197:113815. doi: 10.1016/j.bios.2021.113815. Epub 2021 Nov 18.

Authors

Shah Zada¹, Huiting Lu², Wenhao Dai¹, Songsong Tang¹, Sikandar Khan³, Fan Yang⁴, Yuchun Qiao¹, Pengcheng Fu⁵, Haifeng Dong⁶, Xueji Zhang⁷

Affiliations

¹ Beijing Key Laboratory for Bioengineering and Sensing Technology, Research Centre for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science & Technology Beijing, 30 Xueyuan Road, Beijing, 100083, PR China.
² School of Chemistry and Biological Engineering, University of Science & Technology Beijing, 30 Xueyuan Road, Beijing, 100083, PR China.
³ Department of Biotechnology, Shaheed Benazir Bhutto University, Sheringal, KPK, Pakistan.
⁴ College of Basic Medical Sciences, Shanxi University, Taiyuan, 030001, PR China.
⁵ State Key Laboratory of Marine Resource Utilization in South China Sea Hainan University, 58 Renmin Avenue, Meilan District Haikou, Hainan Province, 570228, PR China.
⁶ Beijing Key Laboratory for Bioengineering and Sensing Technology, Research Centre for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science & Technology Beijing, 30 Xueyuan Road, Beijing, 100083, PR China; Marshall Laboratory of Biomedical Engineering Research Center for Biosensor and Nanotheranostic, School of Biomedical Engineering, Health Science Centre, Shenzhen University, Shenzhen, Guangdong, 518060, PR China. Electronic address: hfdong@ustb.edu.cn.
⁷ Beijing Key Laboratory for Bioengineering and Sensing Technology, Research Centre for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science & Technology Beijing, 30 Xueyuan Road, Beijing, 100083, PR China; Marshall Laboratory of Biomedical Engineering Research Center for Biosensor and Nanotheranostic, School of Biomedical Engineering, Health Science Centre, Shenzhen University, Shenzhen, Guangdong, 518060, PR China. Electronic address: zhangxueji@ustb.edu.cn.

PMID: 34814033
DOI: 10.1016/j.bios.2021.113815

Abstract

Imaging intracellular microRNAs (miRNAs) demonstrated an essential role in exposing their biological and pathological functions. However, the detection of sequence-speciﬁc miRNAs in living cells remains a key challenge. Herein, a facile amplified multiple intracellular miRNAs imaging platform was constructed based on Mo₂B nanosheets (NSs) fluorescence (FL) quenching and hybridization chain reaction (HCR). The Mo₂B NSs demonstrated strong interaction with the hairpin probes (HPs), ssDNA loop, and excellent multiple FL dyes quenching performance, achieving ultralow background signal. After transfection, the HPs recognized specific targets miRNAs, the corresponding HCR was triggered to produce tremendous DNA-miRNA duplex helixes, which dissociated from the surface of the Mo₂B NSs to produce strong FL for miRNAs detection. It realized to image multiple miRNAs biomarkers in different cells to discriminate cancer cells from normal cells owing to the excellent sensitivity, and the regulated expression change of miRNAs in cancer cells was also successfully monitored. The facile and versatile Mo₂B-based FL quenching platform open an avenue to profile miRNAs expression pattern in living cells, and has great applications in miRNAs based biological and biomedical research.

Keywords: Fluorescence quenching; Hybridization chain reaction; MicroRNA; Mo(2)B.

MeSH terms

Biosensing Techniques*
DNA
Fluorescent Dyes
MicroRNAs* / genetics
Nucleic Acid Hybridization

Substances

Fluorescent Dyes
MicroRNAs
DNA