Chemical composition and antimicrobial activity of the essential oil of Ruta chalepensis L. growing wild in Lebanon

Chem Biodivers. 2014 Dec;11(12):1990-7. doi: 10.1002/cbdv.201400109.

Authors

Madona Khoury¹, Didier Stien, Naïm Ouaini, Véronique Eparvier, Nelly Arnold Apostolides, Marc El Beyrouthy

Affiliation

¹ CNRS, Institut de Chimie des Substances Naturelles, UPR 2301, 1 Avenue de la Terrasse, F-91198 Gif-sur-Yvette (phone: +33-1-69823610; fax: +33-1-69823784); Department of Agricultural Sciences, Holy Spirit University of Kaslik, Kaslik, B.P. 446, Jounieh, Lebanon.

PMID: 25491342
DOI: 10.1002/cbdv.201400109

Abstract

The essential oils (EOs) isolated from the fresh aerial parts of Ruta chalepensis L. collected in North Lebanon were obtained by solvent-free microwave extraction (Milestone®), yielding 0.12% EO from both the leaves and a mixture of stems and leaves. The EOs were characterized by GC/MS analysis, and 27 components were identified, which were primarily ketones (88.0-93.2%). The main components were nonan-2-one and undecan-2-one. The antimicrobial activity of the EOs against a Gram-positive and a Gram-negative bacterium, a yeast, and a dermatophyte was evaluated using the broth-microdilution technique and expressed as minimal inhibitory concentration (MIC). The EOs revealed moderate in vitro antifungal activity against Trichophyton rubrum and Candida albicans.

Keywords: Antimicrobial activity; Essential oils; Nonan-2-one; Ruta chalepensis; Undecan-2-one.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Gram-Negative Bacteria / drug effects
Gram-Positive Bacteria / drug effects
Lebanon
Microbial Sensitivity Tests
Oils, Volatile / chemistry*
Oils, Volatile / pharmacology*
Ruta / chemistry*

Substances

Anti-Bacterial Agents
Oils, Volatile