Instantaneous three-dimensional (3D) measurements have been long desired to resolve the spatial structures of turbulent flows and flame. Previous efforts have demonstrated tomography as a promising technique to enable such measurements. To facilitate the practical application, this work investigated four practical aspects for implementing 3D tomographic under the context of volumetric combustion diagnostics. Both numerical simulations and controlled experiments were performed to study: (1) the termination criteria of the inversion algorithm; (2) the effects of regularization and the determination of the optimal regularization factor; (3) the effects of a number of views; and (4) the impact of the resolution of the projection measurements. The results obtained have illustrated the effects of these practical aspects on the accuracy and spatial resolution of volumetric tomography. Furthermore, all these aspects are related to the complexity and implementing cost (both hardware cost and computational cost). Therefore, the results obtained in this work are expected to be valuable for the design and implementation of practical 3D diagnostics.