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Bioactivity against Bursaphelenchus xylophilus: Nematotoxics from essential oils, essential oils fractions and decoction waters

Phytochemistry. 2013 Oct:94:220-8. doi: 10.1016/j.phytochem.2013.06.005. Epub 2013 Jul 2.

Abstract

The Portuguese pine forest has become dangerously threatened by pine wilt disease (PWD), caused by the pinewood nematode (PWN), Bursaphelenchus xylophilus. Synthetic chemicals are the most common pesticides used against phytoparasitic nematodes but its use has negative ecological impacts. Phytochemicals may prove to be environmentally friendly alternatives. Essential oils (EOs) and decoction waters, isolated from 84 plant samples, were tested against B. xylophilus, in direct contact assays. Some successful EOs were fractionated and the fractions containing hydrocarbons or oxygen-containing molecules tested separately. Twenty EOs showed corrected mortalities ⩾96% at 2 μL/mL. These were further tested at lower concentrations. Ruta graveolens, Satureja montana and Thymbra capitata EOs showed lethal concentrations (LC100)<0.4μL/mL. Oxygen-containing molecules fractions showing corrected mortality ⩾96% did not always show LC100 values similar to the corresponding EOs, suggesting additive and/or synergistic relationships among fractions. Nine decoction waters (remaining hydrodistillation waters) revealed 100% mortality at a minimum concentration of 12.5μL/mL. R. graveolens, S. montana and T. capitata EOs are potential environmentally friendly alternatives for B. xylophilus control given their high nematotoxic properties. Nematotoxic activity of an EO should be taken in its entirety, as its different components may contribute, in distinct ways, to the overall EO activity.

Keywords: 2-Undecanone; Bursaphelenchus xylophilus; Carvacrol; Decoction waters; Essential oils; Hydrocarbon molecules; Nematotoxic; Oxygen-containing molecules; Pinus pinaster; γ-Terpinene.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chemical Fractionation
  • Cluster Analysis
  • Dose-Response Relationship, Drug
  • Host-Parasite Interactions / drug effects
  • Lamiaceae / chemistry
  • Nematoda / drug effects*
  • Nematoda / physiology
  • Oils, Volatile / classification
  • Oils, Volatile / isolation & purification
  • Oils, Volatile / pharmacology*
  • Pinus / parasitology
  • Plant Diseases / parasitology
  • Plant Extracts / chemistry
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology
  • Plant Oils / classification
  • Plant Oils / isolation & purification
  • Plant Oils / pharmacology*
  • Plants / chemistry*
  • Plants / classification
  • Ruta / chemistry
  • Satureja / chemistry
  • Species Specificity
  • Water / chemistry

Substances

  • Oils, Volatile
  • Plant Extracts
  • Plant Oils
  • Water