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A flash-lamp based device for fluorescence detection and identification of individual pollen grains

Rev Sci Instrum. 2013 Mar;84(3):033302. doi: 10.1063/1.4793792.

Abstract

We present a novel optical aerosol particle detector based on Xe flash lamp excitation and spectrally resolved fluorescence acquisition. We demonstrate its performances on three natural pollens acquiring in real-time scattering intensity at two wavelengths, sub-microsecond time-resolved scattering traces of the particles' passage in the focus, and UV-excited fluorescence spectra. We show that the device gives access to a rather specific detection of the bioaerosol particles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aerosols
  • Cost-Benefit Analysis
  • Electronics
  • Environmental Monitoring / methods
  • Equipment Design
  • Fluorescence
  • Lasers
  • Light
  • Normal Distribution
  • Pollen / chemistry*
  • Principal Component Analysis
  • Scattering, Radiation
  • Spectrometry, Fluorescence / methods*
  • Surface Properties
  • Ultraviolet Rays

Substances

  • Aerosols