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Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases

J Infect Chemother. 2009 Apr;15(2):62-9. doi: 10.1007/s10156-009-0669-9. Epub 2009 Apr 25.

Abstract

Loop-mediated isothermal amplification (LAMP) is an established nucleic acid amplification method offering rapid, accurate, and cost-effective diagnosis of infectious diseases. This technology has been developed into commercially available detection kits for a variety of pathogens including bacteria and viruses. The current focus on LAMP methodology is as a diagnostic system to be employed in resource-limited laboratories in developing countries, where many fatal tropical diseases are endemic. The combination of LAMP and novel microfluidic technologies such as Lab-on-a-chip may facilitate the realization of genetic point-of-care testing systems to be used by both developed and developing countries in the near future. This review will describe the historical, current, and future developments of such technologies.

Publication types

  • Review

MeSH terms

  • Animals
  • Bacteria / genetics
  • Bacteria / isolation & purification
  • Communicable Diseases / diagnosis*
  • Eukaryota / genetics
  • Eukaryota / isolation & purification
  • Humans
  • Nucleic Acid Amplification Techniques* / economics
  • Nucleic Acid Amplification Techniques* / methods
  • Point-of-Care Systems
  • Time Factors
  • Viruses / genetics
  • Viruses / isolation & purification