Investigation of the effect of salt additives in Protein L affinity chromatography for the purification of tandem single-chain variable fragment bispecific antibodies

SW Chen, D Tan, YS Yang, W Zhang - MAbs, 2020 - Taylor & Francis
Tandem single-chain variable fragment (scFv) bispecific antibodies (bsAb) are one of the
most promising bsAb formats reported thus far. Yet, because of their increased aggregation …

Membrane chromatography for protein purifications from ligand design to functionalization

Z Liu, SR Wickramasinghe, X Qian - Separation Science and …, 2017 - Taylor & Francis
Capto TM L is resin developed by GE Healthcare for purifications of a wide range of antibody
fragments, such as Fabs, single-chain variable fragments (scFv) and domain antibodies (…

Understanding the biosynthesis of human IgM SAM-6 through a combinatorial expression of mutant subunits that affect product assembly and secretion

H Hasegawa, S Wang, E Kast, HT Chou, M Kaur… - Plos one, 2024 - journals.plos.org
… Although the LC isotype of SAM-6 IgM was λ, we inadvertently found that SAM-6 IgM can
be purified using Capto TM L resin (Cytiva) or HiTrap TM protein L resin (Cytiva) that are …

Purificaà § ã oe caracterizaà § ã o do fragmento Fab anti-digoxina obtido pela té cnica de phage display.

AÃ Inocencio - teses.usp.br
A digoxina é um dos medicamentos indicados para o tratamento de falência cardÃaca.
Possui janela terapêutica estreita, sendo responsável por casos de intoxicação. O único …

[PDF][PDF] Specific enzymatic digestion and fragment isolation for the critical quality attribute assessment of IgG1

MA Rappo - 2017 - digitalcollection.zhaw.ch
Recent ICH quality guideline updates introduced the enhanced drug development which is
based on Quality by Design approach. For this a Quality Risk Management has to be …