Antibody engineering: comparison of bacterial, yeast, insect and mammalian expression systems

R Verma, E Boleti, AJT George - Journal of immunological methods, 1998 - Elsevier
Engineered antibody molecules, and their fragments, are being increasingly exploited as
scientific and clinical tools. However, one factor that can limit the applicability of this technology …

Miniantibodies

JV Schaefer, P Lindner, A Plückthun - Antibody engineering, 2010 - Springer
Recombinant antibodies have become a standard component of research, diagnostics, and
therapy. In the development of recombinant antibodies – irrespective of the final format – a …

Engineering antibody molecules

R Verma, E Boleti - Diagnostic and Therapeutic Antibodies, 2000 - Springer
Advances in PCR techniques and the increase of the antibody V region sequences in the
database have boosted developments in the field of antibody engineering. The V region genes …

Immunoglobulin and T Cell Receptor Genes: IMGT® and the Birth and Rise of Immunoinformatics

MP Lefranc - Frontiers in immunology, 2014 - frontiersin.org
IMGT ® , the international ImMunoGeneTics information system ® 1 , (CNRS and Université
Montpellier 2) is the global reference in immunogenetics and immunoinformatics. By its …

[HTML][HTML] Immunoglobulins: 25 years of immunoinformatics and IMGT-ONTOLOGY

MP Lefranc - Biomolecules, 2014 - mdpi.com
IMGT ® , the international ImMunoGeneTics information system ® (CNRS and Montpellier
University) is the global reference in immunogenetics and immunoinformatics. By its creation …

Research advances in genetic engineering of plantibody

J Zeng, Y Fu, X Yu, X Jiang, H Huang - Chinese science bulletin, 1999 - Springer
The combination of engineering antibody and plant biotechnology creates the plantibody. It
has been reported that the engineering antibodies expressed in plant, no matter whether …

Human antibody neutralizes severe Fever with thrombocytopenia syndrome virus, an emerging hemorrhagic Fever virus

X Guo, L Zhang, W Zhang, Y Chi, X Zeng… - Clinical and vaccine …, 2013 - Am Soc Microbiol
Severe fever with thrombocytopenia syndrome virus (SFTSV), a newly discovered member of
the Bunyaviridae family, is the causative agent of an emerging hemorrhagic fever, SFTS, in …

Analysis of monoclonal antibody sequence and post-translational modifications by time-controlled proteolysis and tandem mass spectrometry

L Zhang, AM English, DL Bai, SA Ugrin… - Molecular & Cellular …, 2016 - ASBMB
Methodology for sequence analysis of ∼150 kDa monoclonal antibodies (mAb), including
location of post-translational modifications and disulfide bonds, is described. Limited …

Correlation between in vitro stability and in vivo performance of anti-GCN4 intrabodies as cytoplasmic inhibitors

A Wörn, AA Der Maur, D Escher, A Honegger… - Journal of Biological …, 2000 - ASBMB
A cellular assay system for measuring the activity of cytoplasmically expressed anti-GCN4
scFv fragments directed against the Gcn4p dimerization domain was established in the …

Cloning, expression, and characterization of recombinant Fab antibodies against dioxin

N Lee, CK Holtzapple, LH Stanker - Journal of Agricultural and …, 1998 - ACS Publications
Using two hybridoma cell lines (DD1 and DD3) secreting anti-dioxin monoclonal antibodies
as a source for messenger RNA and cDNA, light and heavy chain gene fragments of Fab …