Europe PMC
Nothing Special   »   [go: up one dir, main page]

Europe PMC requires Javascript to function effectively.

Either your web browser doesn't support Javascript or it is currently turned off. In the latter case, please turn on Javascript support in your web browser and reload this page.

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Cytosolic intermediates for cell wall biosynthesis and degradation control inducible beta-lactam resistance in gram-negative bacteria.

Author information

Affiliations

  • 1. Centre d'Ingénierie des Protéines, Université de Liège, Belgium.
      Authors
    • Jacobs C 1
    (1 author)

Cell, 01 Mar 1997, 88(6):823-832
https://doi.org/10.1016/s0092-8674(00)81928-5 PMID: 9118225 

Abstract 

beta-lactam induction of chromosomal beta-lactamase in gram-negative bacteria requires the transcriptional regulator AmpR and the transport of murein breakdown products (muropeptides) into the cytoplasm. In vitro transcription shows that purified AmpR acts as an activator for ampC beta-lactamase synthesis. The murein precursor, UDP-MurNAc-pentapeptide, decreases AmpR-mediated transcriptional activation in vitro, but has no effect on an AmpR(G102E) mutant that mediates constitutive activation of ampC in vivo. Addition of the muropeptide, anhMurNAc-tripeptide, which accumulates in beta-lactamase-overproducing mutants, counteracts the negative effect of UDP-MurNAc-pentapeptide, restoring the innate ability of AmpR to induce ampC expression in vitro. Cytosolic intermediates of murein biosynthesis and degradation thus act antagonistically to control beta-lactamase expression, thereby operating as a cell-wall sensing device.

Full text links 

Read article at publisher's site: https://doi.org/10.1016/s0092-8674(00)81928-5

Read article for free, from open access legal sources, via Unpaywall: http://www.cell.com/article/S0092867400819285/pdfUnpaywall

References 

Articles referenced by this article (35)

Show 10 more references (10 of 35)

Citations & impact 

Impact metrics

202
Citations
Jump to Citations

Citations of article over time

Alternative metrics

Altmetric item for https://www.altmetric.com/details/413523
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/413523

Article citations

Go to all (202) article citations

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.