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Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections.

Author information

Affiliations

  • 1. Institute of Virology, University of Bonn Medical Centre, Bonn, Germany.
      Authors
    • Corman VM 1
    (1 author)

ORCIDs linked to this article

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Euro Surveillance : Bulletin Europeen sur les Maladies Transmissibles = European Communicable Disease Bulletin, 06 Dec 2012, 17(49):20334
https://doi.org/10.2807/ese.17.49.20334-en PMID: 23231891 

Abstract 

We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent patient serum.

Full text links 

Read article at publisher's site: https://doi.org/10.2807/ese.17.49.20334-en

Read article for free, from open access legal sources, via Unpaywall: https://www.eurosurveillance.org/deliver/fulltext/eurosurveillance/17/49/art20334-en.pdf?itemId=%2Fcontent%2F10.2807%2Fese.17.49.20334-en&mimeType=pdf&containerItemId=content/eurosurveillanceUnpaywall

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