Europe PMC
Nothing Special   »   [go: up one dir, main page]

Europe PMC requires Javascript to function effectively.

Either your web browser doesn't support Javascript or it is currently turned off. In the latter case, please turn on Javascript support in your web browser and reload this page.

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Successful serial recloning in the mouse over multiple generations.

Author information

Affiliations

  • 1. RIKEN Center for Developmental Biology, Kobe 650-0047, Japan.
      Authors
    • Wakayama S 1
    (1 author)

ORCIDs linked to this article

Cell Stem Cell, 01 Mar 2013, 12(3):293-297
https://doi.org/10.1016/j.stem.2013.01.005 PMID: 23472871 

Abstract 

Previous studies of serial cloning in animals showed a decrease in efficiency over repeated iterations and a failure in all species after a few generations. This limitation led to the suggestion that repeated recloning might be inherently impossible because of the accumulation of lethal genetic or epigenetic abnormalities. However, we have now succeeded in carrying out repeated recloning in the mouse through a somatic cell nuclear transfer method that includes a histone deacetylase inhibitor. The cloning efficiency did not decrease over 25 generations, and, to date, we have obtained more than 500 viable offspring from a single original donor mouse. The reprogramming efficiency also did not increase over repeated rounds of nuclear transfer, and we did not see the accumulation of reprogramming errors or clone-specific abnormalities. Therefore, our results show that repeated iterative recloning is possible and suggest that, with adequately efficient techniques, it may be possible to reclone animals indefinitely.

Full text links 

Read article at publisher's site: https://doi.org/10.1016/j.stem.2013.01.005

Read article for free, from open access legal sources, via Unpaywall: http://www.cell.com/article/S1934590913000088/pdfUnpaywall

References 

Articles referenced by this article (26)

Show 10 more references (10 of 26)

Citations & impact 

Impact metrics

47
Citations
Jump to Citations
1
Data citation
Jump to Data

Citations of article over time

Alternative metrics

Altmetric item for https://www.altmetric.com/details/1287117
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/1287117

Smart citations by scite.ai
Smart citations by scite.ai include citation statements extracted from the full text of the citing article. The number of the statements may be higher than the number of citations provided by EuropePMC if one paper cites another multiple times or lower if scite has not yet processed some of the citing articles.
Explore citation contexts and check if this article has been supported or disputed.
https://scite.ai/reports/10.1016/j.stem.2013.01.005

Supporting
Mentioning
Contrasting
0
36
0

Article citations

Go to all (47) article citations

Other citations

Data 

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.