Mechanism of transcriptional activation by Sp1: evidence for coactivators.

Abstract

In reconstituted reactions, Sp1 stimulates transcription at TATA-containing promoters in the presence of semipurified TFIID fractions from either human or Drosophila cells, but is unable to do so when these fractions are replaced by purified, cloned Drosophila or yeast TFIID. Our findings with Sp1 and CTF suggest that partially purified TFIID fractions from human and Drosophila cells contain coactivators that are dispensable for basal transcription but are required as molecular adaptors between trans-activators and the general transcription initiation machinery. Experiments using cloned TFIID proteins suggest that these coactivators function through the amino-terminal portion of TFIID and that coactivator-TFIID interactions are species specific. At promoters lacking a TATA box, an additional activity distinct from coactivators is required for Sp1 activation of transcription.

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References

Articles referenced by this article (46)

Functional cooperativity between transcription factors UBF1 and SL1 mediates human ribosomal RNA synthesis.
Bell SP, Learned RM, Jantzen HM, Tjian R
Science, (4870):1192-1197 1988
MED: 3413483
Molecular mechanisms governing species-specific transcription of ribosomal RNA.
Bell SP, Pikaard CS, Reeder RH, Tjian R
Cell, (3):489-497 1989
MED: 2805069
Assembly of alternative multiprotein complexes directs rRNA promoter selectivity.
Bell SP, Jantzen HM, Tjian R
Genes Dev, (6):943-954 1990
MED: 2384215
Purification and biochemical characterization of the promoter-specific transcription factor, Sp1.
Briggs MR, Kadonaga JT, Bell SP, Tjian R
Science, (4772):47-52 1986
MED: 3529394
Function of a yeast TATA element-binding protein in a mammalian transcription system.
Buratowski S, Hahn S, Sharp PA, Guarente L
Nature, (6177):37-42 1988
MED: 3290687
Five intermediate complexes in transcription initiation by RNA polymerase II.
Buratowski S, Hahn S, Guarente L, Sharp PA
Cell, (4):549-561 1989
MED: 2917366
Cloning of the gene encoding the yeast protein BTF1Y, which can substitute for the human TATA box-binding factor
Cavallini
1989
A yeast activity can substitute for the HeLa cell TATA box factor.
Cavallini B, Huet J, Plassat JL, Sentenac A, Egly JM, Chambon P
Nature, (6177):77-80 1988
MED: 3290688
Analysis of Sp1 in vivo reveals multiple transcriptional domains, including a novel glutamine-rich activation motif.
Courey AJ, Tjian R
Cell, (5):887-898 1988
MED: 3142690
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
Dignam JD, Lebovitz RM, Roeder RG
Nucleic Acids Res, (5):1475-1489 1983
MED: 6828386

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TATA-box-binding protein(UniProt - P20227)

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References

Functional cooperativity between transcription factors UBF1 and SL1 mediates human ribosomal RNA synthesis.

Molecular mechanisms governing species-specific transcription of ribosomal RNA.

Assembly of alternative multiprotein complexes directs rRNA promoter selectivity.

Purification and biochemical characterization of the promoter-specific transcription factor, Sp1.

Function of a yeast TATA element-binding protein in a mammalian transcription system.

Five intermediate complexes in transcription initiation by RNA polymerase II.

Cloning of the gene encoding the yeast protein BTF1Y, which can substitute for the human TATA box-binding factor

A yeast activity can substitute for the HeLa cell TATA box factor.

Analysis of Sp1 in vivo reveals multiple transcriptional domains, including a novel glutamine-rich activation motif.

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Citations & impact

Impact metrics

Citations of article over time

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Article citations

Decoding high mobility group A2 protein expression regulation and implications in human cancers.

Molecular Cloning and Functional Characterization of the 5' Regulatory Region of the SLC11A1 Gene from Yaks.

Transcription and chromatin regulation by TAF4b during cellular quiescence of developing prospermatogonia.

Regulation of the RNA polymerase II pre-initiation complex by its associated coactivators.

Stress Triggers Expression of Bovine Herpesvirus 1 Infected Cell Protein 4 (bICP4) RNA during Early Stages of Reactivation from Latency in Pharyngeal Tonsil.

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Mechanism of transcriptional activation by Sp1: evidence for coactivators.

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Cloning of the gene encoding the yeast protein BTF1Y, which can substitute for the human TATA box-binding factor

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