High-resolution map and imprinting analysis of the Gtl2-Dnchc1 domain on mouse chromosome 12.

Abstract

The imprinted Dlk1-Dio3 region on mouse chromosome 12 contains six imprinted genes and a number of maternally expressed snoRNAs and miRNAs. Here we present a high-resolution sequence analysis of the 1.1-Mb segment telomeric to Gtl2 in mouse and a homology comparison to the human. Ppp2r5c and Dnchc1 at the telomeric end of the analyzed sequence are biallelically expressed, suggesting that the imprinted domain does not extend beyond the paternally expressed Dio3 gene. RT-PCR experiments support the predicted presence of a maternally expressed intergenic transcript(s) encompassing Gtl2, Rian, and Mirg. These maternally expressed genes, and also the intergenic transcript(s), show pronounced expression in the adult mouse brain, whereas the paternally transcribed Dio3 and the nonimprinted Ppp2r5c and Dnchc1 are expressed in different tissues. Hence, tissue-specific coregulation of maternally expressed genes might be an important feature of this domain.

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References

Articles referenced by this article (43)

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DNA-directed RNA polymerase subunit(UniProt - A0A0R4J0V5)
Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit(UniProt - A0A1Y7VIR0)
Protein phosphatase 2, regulatory subunit B', gamma(UniProt - A0A1Y7VJC8)
EGF-like domain-containing protein(UniProt - E9PVN3)
EGF-like domain-containing protein(UniProt - B4YB48)

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Mouse Genome Informatics (MGI)

http://www.informatics.jax.org/reference/16309881

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Medical Research Council (1)

The regulation of imprinted genes by non-coding RNAs
Professor Anne Ferguson-Smith, University of Cambridge
Grant ID: G0400156
15 publications

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References

The callipyge locus: evidence for the trans interaction of reciprocally imprinted genes.

Parental origin-specific developmental defects in mice with uniparental disomy for chromosome 12.

Roles for genomic imprinting and the zygotic genome in placental development.

Search for imprinted regions on chromosome 14: comparison of maternal and paternal UPD cases with cases of chromosome 14 deletion.

Parental origin effects in human trisomy for chromosome 14q: implications for genomic imprinting.

A large imprinted microRNA gene cluster at the mouse Dlk1-Gtl2 domain.

Imprinted microRNA genes transcribed antisense to a reciprocally imprinted retrotransposon-like gene.

Genomic imprinting contributes to thyroid hormone metabolism in the mouse embryo.

The gene locus encoding iodothyronine deiodinase type 3 (Dio3) is imprinted in the fetus and expresses antisense transcripts.

The callipyge mutation enhances the expression of coregulated imprinted genes in cis without affecting their imprinting status.

Citations & impact

Impact metrics

Citations of article over time

Article citations

Identification of candidate causal variants and target genes at 41 breast cancer risk loci through differential allelic expression analysis.

LncRNAs in the Dlk1-Dio3 Domain Are Essential for Mid-Embryonic Heart Development.

Epigenetic control and genomic imprinting dynamics of the Dlk1-Dio3 domain.

Meg8-DMR as the Secondary Regulatory Region Regulates the Expression of MicroRNAs While It Does Not Affect Embryonic Development in Mice.

Alterations in the hepatocyte epigenetic landscape in steatosis.

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Mouse Genome Informatics (MGI)

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Medical Research Council (1)

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Search life-sciences literature (45,094,167 articles, preprints and more)

High-resolution map and imprinting analysis of the Gtl2-Dnchc1 domain on mouse chromosome 12.

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