Europe PMC
Nothing Special   »   [go: up one dir, main page]

Europe PMC requires Javascript to function effectively.

Either your web browser doesn't support Javascript or it is currently turned off. In the latter case, please turn on Javascript support in your web browser and reload this page.

This website requires cookies, and the limited processing of your personal data in order to function. By using the site you are agreeing to this as outlined in our privacy notice and cookie policy.

Pseudomonas aeruginosa mutations in lasI and rhlI quorum sensing systems result in milder chronic lung infection.

Author information

Affiliations

  • 1. Department of Clinical Microbiology, 9301 Rigshospitalet, Juliane Maries Vej 22, DK-2100, Copenhagen Ø, Denmark1.
      Authors
    • Wu H 1
    • Song Z 1, 2
    • Høiby N 1
    (3 authors)
  • 2. Department of Biological Sciences, Florida International University, University Park, Miami, FL 33199, USA4.
      Authors
    • Song Z 1, 2
    • Mathee K 2
    (2 authors)
  • 3. Institute of Microbiology, Technical University of Denmark, DK-2800, Lyngby, Denmark2.
      Authors
    • Givskov M 3
    (1 author)
  • 4. Hygiene Institute, University of Tübingen,D-72074, Tübingen, Germany3.
      Authors
    • Doring G 4
    • Worlitzsch D 4
    (2 authors)
  • 5. Bartholin Institute, Kommunehospitalet, Copenhagen, Denmark5.
      Authors
    • Rygaard J 5
    (1 author)

ORCIDs linked to this article

Microbiology (Reading, England), 01 May 2001, 147(Pt 5):1105-1113
https://doi.org/10.1099/00221287-147-5-1105 PMID: 11320114 

Abstract 

To understand the importance of quorum sensing in chronic Pseudomonas aeruginosa lung infection, the in vivo pathogenic effects of the wild-type P. aeruginosa PAO1 and its double mutant, PAO1 lasI rhlI, in which the signal-generating parts of the quorum sensing systems are defective were compared. The rat model of P. aeruginosa lung infection was used in the present study. The rats were killed on days 3, 7, 14 and 28 after infection with the P. aeruginosa strains. The results showed that during the early stages of infection, the PAO1 double mutant induced a stronger serum antibody response, higher production of pulmonary interferon gamma, and more powerful blood polymorphonuclear leukocyte (PMN) chemiluminescence compared to its wild-type counterpart. On days 14 and 28 post-infection, significantly milder lung pathology, a reduction in the number of mast cells present in the lung foci, a reduced number of lung bacteria, and minor serum IgG and IgG1 responses but increased lung interferon gamma production were detected in the group infected with the PAO1 double mutant when compared with the PAO1-infected group. Delayed immune responses were observed in the PAO1-infected group and they might be associated with the production of virulence factors that are controlled by the quorum sensing systems. The conclusion of this study is that functional lasI and rhlI genes of P. aeruginosa PAO1 play a significant role during lung infection.

Full text links 

Read article at publisher's site: https://doi.org/10.1099/00221287-147-5-1105

Read article for free, from open access legal sources, via Unpaywall: http://mic.microbiologyresearch.org/deliver/fulltext/micro/147/5/1471105a.pdf?itemId=/content/journal/micro/10.1099/00221287-147-5-1105&mimeType=pdf&isFastTrackArticle=Unpaywall

Free to read at mic.sgmjournals.org
http://mic.sgmjournals.org/cgi/content/abstract/147/5/1105

Subscription required at mic.sgmjournals.org
http://mic.sgmjournals.org/cgi/content/full/147/5/1105

Citations & impact 

Impact metrics

93
Citations
Jump to Citations

Citations of article over time

Alternative metrics

Altmetric item for https://www.altmetric.com/details/120473255
Altmetric
Discover the attention surrounding your research
https://www.altmetric.com/details/120473255

Article citations

Go to all (93) article citations

Similar Articles 

To arrive at the top five similar articles we use a word-weighted algorithm to compare words from the Title and Abstract of each citation.