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Genomewide analysis of mRNA processing in yeast using splicing-specific microarrays.

Author information

Affiliations

  • 1. Department of Molecular, Cell, and Developmental Biology, Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California, Santa Cruz, CA 95064, USA.
      Authors
    • Clark TA 1
    (1 author)

ORCIDs linked to this article

Science, 01 May 2002, 296(5569):907-910
https://doi.org/10.1126/science.1069415 PMID: 11988574 

Abstract 

Introns interrupt almost every eukaryotic protein-coding gene, yet how the splicing apparatus interprets the genome during messenger RNA (mRNA) synthesis is poorly understood. We designed microarrays to distinguish spliced from unspliced RNA for each intron-containing yeast gene and measured genomewide effects on splicing caused by loss of 18 different mRNA processing factors. After accommodating changes in transcription and decay by using gene-specific indexes, functional relationships between mRNA processing factors can be identified through their common effects on spliced and unspliced RNA. Groups of genes with different dependencies on mRNA processing factors are also apparent. Quantitative polymerase chain reactions confirm the array-based finding that Prp17p and Prp18p are not dispensable for removal of introns with short branchpoint-to-3' splice site distances.

Full text links 

Read article at publisher's site: https://doi.org/10.1126/science.1069415

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Funding 

Funders who supported this work.

NCI NIH HHS (1)

NIGMS NIH HHS (1)