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Metabonomics Techniques and Applications to Pharmaceutical Research & Development

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Abstract

In this review, the background to the approach known as metabonomics is provided, giving a brief historical perspective and summarizing the analytical and statistical techniques used. Some of the major applications of metabonomics relevant to pharmaceutical Research & Development are then reviewed including the study of various influences on metabolism, such as diet, lifestyle, and other environmental factors. The applications of metabonomics in drug safety studies are explained with special reference to the aims and achievements of the Consortium for Metabonomic Toxicology. Next, the role that metabonomics might have in disease diagnosis and therapy monitoring is provided with some examples, and the concept of pharmacometabonomics as a way of predicting an individual's response to treatment is highlighted. Some discussion is given on the strengths and weaknesses, opportunities of, and threats to metabonomics.

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Abbreviations

CE:

capillary electrophoresis

CLOUDS:

classification of unknowns by density superposition

COMET:

Consortium for Metabonomic Toxicology

COSY:

correlation spectroscopy

CPMG:

Carr-Purcell-Meiboom-Gill (an NMR pulse sequence)

CSF:

cerebrospinal fluid

DA:

discriminant analysis

FT:

Fourier transform

GC:

gas chromatography

HPLC:

high-performance liquid chromatography

IR:

infrared

LC:

liquid chromatography

LIMS:

laboratory information management system

MAS:

magic angle spinning

MS:

mass spectrometry

NMR:

nuclear magnetic resonance

OSC:

orthogonal signal correction

PCA:

principal component analysis

PLS:

partial least squares

QC:

quality control

SHY:

statistical heterospectroscopy

SMRS:

Standard Metabolic Reporting Structures

STOCSY:

statistical total correlation spectroscopy

TOCSY:

total correlation spectroscopy

TOF:

time of flight

TSP:

trimethylsilylpropionic acid sodium salt

UPLC:

ultra-performance liquid chromatography

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  1. Biological Chemistry, Biomedical Sciences Division, Imperial College London, Sir Alexander Fleming Building, South Kensington, London, SW7 2AZ, UK

    John C. Lindon, Elaine Holmes & Jeremy K. Nicholson

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  1. John C. Lindon
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Lindon, J.C., Holmes, E. & Nicholson, J.K. Metabonomics Techniques and Applications to Pharmaceutical Research & Development. Pharm Res 23, 1075–1088 (2006). https://doi.org/10.1007/s11095-006-0025-z

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